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The map label for this gene is lacZ [H]

Identifier: 158314966

GI number: 158314966

Start: 3721799

End: 3724198

Strand: Direct

Name: lacZ [H]

Synonym: Franean1_3159

Alternate gene names: 158314966

Gene position: 3721799-3724198 (Clockwise)

Preceding gene: 158314957

Following gene: 158314967

Centisome position: 41.44

GC content: 66.0

Gene sequence:

>2400_bases
GTGTGGGATGAGGTCAGGCTCCCTCATGACGCGATGGTGGCTCGTGAACGTAACCGTTCCGACGCCGCGGCGGGCCAGCG
CGGCTTCTACCCGGCTGGGGCGTACCAGTACAGGAAGTCGTTCTTCGTGCCGGAGGAGTACCGGAACCGGCGCGTCACCT
TCGAGTTCGAGGGTGTCTACCGGAGCGCCAGGGTTTTTCTCAATGGTGGCCTCGCCGGACAGCATGCCTACGGCTACTCC
CACTTCTACGTCCGCGCCGACCATTTCCTGAAGTACAACGAGGACAACGAGATCCTGGTGGAGGCACACAGCGCCGATGA
CACCCGCTGGTACTCGGGTGGTGGCCTGTACCGCAACACGAAGCTCATCGTCGGCGATCTCGTGCACATCGGCCTGGACG
GGGTGAAGGTCACGACGCCGGCCATCGACGCGGACCTTGCCCTAGTCGCGGTGGCGACGCAGGTGCACAACGAGTCCTCG
GTCACCCGGACCGTCGAGGTGACCACCGAGATCGTGGGCGCCGACGGTGTCGTCGTGGTCCGTGACGTCGCCCCGCTCAC
CCTGTTCACGGACGATCCGGTCACCGTACGCCAGCGGCTGCCGGTGCCGCGGCCGCAGCTGTGGGGAGTGGAGCACCCGC
ACCTCTACACCTGCCGGACCAGAGTGACGGCGGACGGAGAGCTCCTGGATGAGGAGGCCACCCGTTTCGGTGTCCGGTCG
CTCACCGTCGATCCGCACTGGGGTCTGCGCATCAATGGTGGGGTGGTGAACCTCCGCGGCGCGTGCATCCACCACGACAA
CGGCGTGATCGGTGCCGCCACGATAGACCGGGCCGAACAGCGCCGGGTCGAGATCCTGAAACAGGCCGGTTTCAACGCCA
TCCGTAGTTCCCACAATCCGATCAGCAAGGCCCTCCTCGACGCCTGCGACCGGCTCGGCATGCTCGTAATCGACGAGTTG
TTCGACGCATGGACCCGGTCGAAGGTCGCGCAGGACTACGCCCTCGACTTCCCCCTCTGGTGGAAGTCGGACGTGCAGGC
GATGGTCGACAAGGACTTCAACCACCCCTGCGTGATCCTCTACTCGATCGGGAACGAGATCCCGGAGACAGGCACCGCTG
CCGGCGCGGCGATCAGCCGCCAGCTCGCCGAGAGGATCCGAGCCATCGACGACACCCGTTTCGTCACGAACGGCGTCAAC
GGCCTTCTCGCCGGCGGCCCCGAGCTGCTCGCGTCGTTCTCCAGCGGTGCTCGGGAGAAAAGCAGCGAGGCGGGCGAGGC
GGTGGACGTCAACGCGTTCATGAACAGGTTCCGCGAGTTTATGCCGATCCTCATGGCCTCCGAAATGGTCGGTTCGAAGA
CCGCGGAGTCGATGGCCTGCCTGGACGTCGCCGGCTACAACTACCTGGAGTCACGGTACGAGCTGGACCGAACACTGTTC
CCGAACCGGGTGATCGTGGGGACCGAGACCTACCCGTCGGAGATCGACAGGAACTGGCGGCTCGTCCAGGACAACAGCCA
CGTCATCGGTGACTTCACCTGGACCGGCTGGGACTATCTCGGCGAACCAGGAATCGGGCGGATCGAGTACCAAGGCGACG
AGGAAAACGCCAGCACCTCCCCATCCCACGGCAGTTATCCGTGGCTGACCGCGTGGTGCGGCGACATCGACATCACCGGC
CACCGCCGACCGGCCTCCTACTACCGCGAGATCGTGTTCGGCCTACGCAGCGAGCCCTACATAGCCGTGCACCGCCCCGA
CCGCTACGGCCAGCCAGTTACGGTGGCGATGTGGTGGTCGTGGAGTGACGCGATCTCCAGCTGGTCCTGGGACGGCCACG
AGACCAGACCGGTGCGGGTGGAGGTTTACTCGGCCGCCGACGAGGTCGAACTTTTGGTCAACGGCCGGCTGATCGGTACC
GTCCCGGCGGGGGAGAAGAACCGGTTTAAGGCCGAGTTCGACACCGTTTACGAACCCGGCGAAATCGTCGCTGTCGCCTA
CACCGCTGGCCGCGAGACCGGACGCACCCTGCTGCGCTCGGCGACCGGCGAGGTCCGCCTCGCCGTCGCCGCCGACCGCA
CCGACATCGTCGCCGACGACACCGACCTTGCCTACATCGCCATCACCCTCGTCGACGAGGCCGGCAACCTCTACAACACC
GCCGACCGCACGGTCGCCGTCGAGGTGGCGGGACCCGGCGTGCTGCAGGGCTTCGGTAGCGCGGACCCGAAAACGGAAGA
GAACTTCTTCGATACCACCCGCGCCACCTTCGACGGCCGGGCACTCGCCGTCATCCGCCCCACCGCCCCCGGCACGATCA
CCGTGACCCTCACCGCGCAGGGATGCGAGCCCTCCACTATCCGCATCGAAGCCGAACTTACGGCACGCTCAGGCGAATGA

Upstream 100 bases:

>100_bases
AGCCGGGAGAGCTCGGTTGATCCGTTTATCGTCCAACGATGGCTGGTTCTTTCGCCAGAGGGCTGACCCTTTCGCGGAGC
TGTTAGGCAGGGCTGACGCC

Downstream 100 bases:

>100_bases
CGGCCCTCCGTGGCCCAAGATCAGCGAACGGAGTCCGGCGCCGCTCCAGCTGACTCATCAGTATGGAGTCGCAGATGACA
TCCGACAGCGCCCGAAGTCC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 799; Mature: 799

Protein sequence:

>799_residues
MWDEVRLPHDAMVARERNRSDAAAGQRGFYPAGAYQYRKSFFVPEEYRNRRVTFEFEGVYRSARVFLNGGLAGQHAYGYS
HFYVRADHFLKYNEDNEILVEAHSADDTRWYSGGGLYRNTKLIVGDLVHIGLDGVKVTTPAIDADLALVAVATQVHNESS
VTRTVEVTTEIVGADGVVVVRDVAPLTLFTDDPVTVRQRLPVPRPQLWGVEHPHLYTCRTRVTADGELLDEEATRFGVRS
LTVDPHWGLRINGGVVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSSHNPISKALLDACDRLGMLVIDEL
FDAWTRSKVAQDYALDFPLWWKSDVQAMVDKDFNHPCVILYSIGNEIPETGTAAGAAISRQLAERIRAIDDTRFVTNGVN
GLLAGGPELLASFSSGAREKSSEAGEAVDVNAFMNRFREFMPILMASEMVGSKTAESMACLDVAGYNYLESRYELDRTLF
PNRVIVGTETYPSEIDRNWRLVQDNSHVIGDFTWTGWDYLGEPGIGRIEYQGDEENASTSPSHGSYPWLTAWCGDIDITG
HRRPASYYREIVFGLRSEPYIAVHRPDRYGQPVTVAMWWSWSDAISSWSWDGHETRPVRVEVYSAADEVELLVNGRLIGT
VPAGEKNRFKAEFDTVYEPGEIVAVAYTAGRETGRTLLRSATGEVRLAVAADRTDIVADDTDLAYIAITLVDEAGNLYNT
ADRTVAVEVAGPGVLQGFGSADPKTEENFFDTTRATFDGRALAVIRPTAPGTITVTLTAQGCEPSTIRIEAELTARSGE

Sequences:

>Translated_799_residues
MWDEVRLPHDAMVARERNRSDAAAGQRGFYPAGAYQYRKSFFVPEEYRNRRVTFEFEGVYRSARVFLNGGLAGQHAYGYS
HFYVRADHFLKYNEDNEILVEAHSADDTRWYSGGGLYRNTKLIVGDLVHIGLDGVKVTTPAIDADLALVAVATQVHNESS
VTRTVEVTTEIVGADGVVVVRDVAPLTLFTDDPVTVRQRLPVPRPQLWGVEHPHLYTCRTRVTADGELLDEEATRFGVRS
LTVDPHWGLRINGGVVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSSHNPISKALLDACDRLGMLVIDEL
FDAWTRSKVAQDYALDFPLWWKSDVQAMVDKDFNHPCVILYSIGNEIPETGTAAGAAISRQLAERIRAIDDTRFVTNGVN
GLLAGGPELLASFSSGAREKSSEAGEAVDVNAFMNRFREFMPILMASEMVGSKTAESMACLDVAGYNYLESRYELDRTLF
PNRVIVGTETYPSEIDRNWRLVQDNSHVIGDFTWTGWDYLGEPGIGRIEYQGDEENASTSPSHGSYPWLTAWCGDIDITG
HRRPASYYREIVFGLRSEPYIAVHRPDRYGQPVTVAMWWSWSDAISSWSWDGHETRPVRVEVYSAADEVELLVNGRLIGT
VPAGEKNRFKAEFDTVYEPGEIVAVAYTAGRETGRTLLRSATGEVRLAVAADRTDIVADDTDLAYIAITLVDEAGNLYNT
ADRTVAVEVAGPGVLQGFGSADPKTEENFFDTTRATFDGRALAVIRPTAPGTITVTLTAQGCEPSTIRIEAELTARSGE
>Mature_799_residues
MWDEVRLPHDAMVARERNRSDAAAGQRGFYPAGAYQYRKSFFVPEEYRNRRVTFEFEGVYRSARVFLNGGLAGQHAYGYS
HFYVRADHFLKYNEDNEILVEAHSADDTRWYSGGGLYRNTKLIVGDLVHIGLDGVKVTTPAIDADLALVAVATQVHNESS
VTRTVEVTTEIVGADGVVVVRDVAPLTLFTDDPVTVRQRLPVPRPQLWGVEHPHLYTCRTRVTADGELLDEEATRFGVRS
LTVDPHWGLRINGGVVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSSHNPISKALLDACDRLGMLVIDEL
FDAWTRSKVAQDYALDFPLWWKSDVQAMVDKDFNHPCVILYSIGNEIPETGTAAGAAISRQLAERIRAIDDTRFVTNGVN
GLLAGGPELLASFSSGAREKSSEAGEAVDVNAFMNRFREFMPILMASEMVGSKTAESMACLDVAGYNYLESRYELDRTLF
PNRVIVGTETYPSEIDRNWRLVQDNSHVIGDFTWTGWDYLGEPGIGRIEYQGDEENASTSPSHGSYPWLTAWCGDIDITG
HRRPASYYREIVFGLRSEPYIAVHRPDRYGQPVTVAMWWSWSDAISSWSWDGHETRPVRVEVYSAADEVELLVNGRLIGT
VPAGEKNRFKAEFDTVYEPGEIVAVAYTAGRETGRTLLRSATGEVRLAVAADRTDIVADDTDLAYIAITLVDEAGNLYNT
ADRTVAVEVAGPGVLQGFGSADPKTEENFFDTTRATFDGRALAVIRPTAPGTITVTLTAQGCEPSTIRIEAELTARSGE

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI48994920, Length=387, Percent_Identity=26.8733850129199, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786539, Length=204, Percent_Identity=32.843137254902, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17510825, Length=385, Percent_Identity=25.1948051948052, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62471735, Length=402, Percent_Identity=25.1243781094527, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24655438, Length=402, Percent_Identity=25.1243781094527, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922582, Length=402, Percent_Identity=25.1243781094527, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24651745, Length=543, Percent_Identity=22.2836095764273, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 88397; Mature: 88397

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDEVRLPHDAMVARERNRSDAAAGQRGFYPAGAYQYRKSFFVPEEYRNRRVTFEFEGVY
CCCCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHCCCCHHHCCCEEEEEECCCE
RSARVFLNGGLAGQHAYGYSHFYVRADHFLKYNEDNEILVEAHSADDTRWYSGGGLYRNT
EEEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEECCCCEEECC
KLIVGDLVHIGLDGVKVTTPAIDADLALVAVATQVHNESSVTRTVEVTTEIVGADGVVVV
EEEEEEEEEECCCCEEEECCCCCCCCEEEEHHHHHCCCCCCEEEEEEEEEEECCCCEEEE
RDVAPLTLFTDDPVTVRQRLPVPRPQLWGVEHPHLYTCRTRVTADGELLDEEATRFGVRS
ECCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCEE
LTVDPHWGLRINGGVVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSSHNP
EEECCCCCEEECCCEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCH
ISKALLDACDRLGMLVIDELFDAWTRSKVAQDYALDFPLWWKSDVQAMVDKDFNHPCVIL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEE
YSIGNEIPETGTAAGAAISRQLAERIRAIDDTRFVTNGVNGLLAGGPELLASFSSGAREK
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEECCCHHHHHHHHCCCCCC
SSEAGEAVDVNAFMNRFREFMPILMASEMVGSKTAESMACLDVAGYNYLESRYELDRTLF
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHCC
PNRVIVGTETYPSEIDRNWRLVQDNSHVIGDFTWTGWDYLGEPGIGRIEYQGDEENASTS
CCEEEEECCCCHHHHCCCCEEEECCCEEEEEEEECCCHHCCCCCCCEEEECCCCCCCCCC
PSHGSYPWLTAWCGDIDITGHRRPASYYREIVFGLRSEPYIAVHRPDRYGQPVTVAMWWS
CCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEC
WSDAISSWSWDGHETRPVRVEVYSAADEVELLVNGRLIGTVPAGEKNRFKAEFDTVYEPG
CHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEECCEEEEECCCCCCCCEEEHHCCCCCCC
EIVAVAYTAGRETGRTLLRSATGEVRLAVAADRTDIVADDTDLAYIAITLVDEAGNLYNT
CEEEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCEEECCCCEEEEEEEEEECCCCCCCC
ADRTVAVEVAGPGVLQGFGSADPKTEENFFDTTRATFDGRALAVIRPTAPGTITVTLTAQ
CCCEEEEEECCCHHCCCCCCCCCCCCCCHHHCHHCCCCCEEEEEEECCCCCEEEEEEEEC
GCEPSTIRIEAELTARSGE
CCCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MWDEVRLPHDAMVARERNRSDAAAGQRGFYPAGAYQYRKSFFVPEEYRNRRVTFEFEGVY
CCCCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHCCCCHHHCCCEEEEEECCCE
RSARVFLNGGLAGQHAYGYSHFYVRADHFLKYNEDNEILVEAHSADDTRWYSGGGLYRNT
EEEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEECCCCEEECC
KLIVGDLVHIGLDGVKVTTPAIDADLALVAVATQVHNESSVTRTVEVTTEIVGADGVVVV
EEEEEEEEEECCCCEEEECCCCCCCCEEEEHHHHHCCCCCCEEEEEEEEEEECCCCEEEE
RDVAPLTLFTDDPVTVRQRLPVPRPQLWGVEHPHLYTCRTRVTADGELLDEEATRFGVRS
ECCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCEE
LTVDPHWGLRINGGVVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSSHNP
EEECCCCCEEECCCEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCH
ISKALLDACDRLGMLVIDELFDAWTRSKVAQDYALDFPLWWKSDVQAMVDKDFNHPCVIL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEE
YSIGNEIPETGTAAGAAISRQLAERIRAIDDTRFVTNGVNGLLAGGPELLASFSSGAREK
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEECCCHHHHHHHHCCCCCC
SSEAGEAVDVNAFMNRFREFMPILMASEMVGSKTAESMACLDVAGYNYLESRYELDRTLF
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHCC
PNRVIVGTETYPSEIDRNWRLVQDNSHVIGDFTWTGWDYLGEPGIGRIEYQGDEENASTS
CCEEEEECCCCHHHHCCCCEEEECCCEEEEEEEECCCHHCCCCCCCEEEECCCCCCCCCC
PSHGSYPWLTAWCGDIDITGHRRPASYYREIVFGLRSEPYIAVHRPDRYGQPVTVAMWWS
CCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEC
WSDAISSWSWDGHETRPVRVEVYSAADEVELLVNGRLIGTVPAGEKNRFKAEFDTVYEPG
CHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEECCEEEEECCCCCCCCEEEHHCCCCCCC
EIVAVAYTAGRETGRTLLRSATGEVRLAVAADRTDIVADDTDLAYIAITLVDEAGNLYNT
CEEEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCEEECCCCEEEEEEEEEECCCCCCCC
ADRTVAVEVAGPGVLQGFGSADPKTEENFFDTTRATFDGRALAVIRPTAPGTITVTLTAQ
CCCEEEEEECCCHHCCCCCCCCCCCCCCHHHCHHCCCCCEEEEEEECCCCCEEEEEEEEC
GCEPSTIRIEAELTARSGE
CCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA