Click here to switch to the map view.

The map label for this gene is caiA [H]

Identifier: 158314530

GI number: 158314530

Start: 3201750

End: 3202907

Strand: Direct

Name: caiA [H]

Synonym: Franean1_2706

Alternate gene names: 158314530

Gene position: 3201750-3202907 (Clockwise)

Preceding gene: 158314524

Following gene: 158314531

Centisome position: 35.65

GC content: 69.43

Gene sequence:

>1158_bases
ATGTTGCATATAACTGCCAGCAGCGAACGGGATCCTCTTCGTGACGATGCGTGCGCATACGCGAAGGAGTTCGAGGCGTG
GCTGACCTCCCGGCCACCCACGCTCGACGCCGCTGCTCGCCCCATCGCCTCGTACGAGAAGCGGGTTGCCGTGACGTCCG
AACTGATGCGCGTCCTCTTCGACGAGGGGTGGGCCCGGTACGGGTGGCCCGAGGAGGCCGGTGGTTTCGGCGGTGACATC
CTGCACCGGGCCGCGATGTGGGAGGCTCTCGCACGTCACGGCCTGCCCGCGATGGCTCTGTTCGAGCACCTGGAGATCCT
GGCGCCGACTCTTGTGGCCATGGGCCGCCCGGACTTCATAGCACAGGTGCTGCCGCGCTTTCTCCGCGGCGAGCAGCGTT
GGTCGCAGGGGTTCTCCGAGCCCGATGCGGGTTCCGATCTCGCCAGCGCCCGCACCCGCGCCGTCGCGGCTGACGGCGTC
CACGTCATCACCGGCCGGAAGATCTGGACGAGCTGGGCGTCCTACGCCACCTGGTGTCTGGTCCTCGCCCGGACCGGGAC
CCTGGAGTCTCGCCACCGCGGCCTGACCGCCTTCGCCGTCGACCTGCGCGCCCCCGGGGTCGAGGTCAGGCCGATCGAGC
AGGCGAACGGCACCGACGAGCTGGCCGAGGTCACCTTCGACGAGGTCCCCGTCGGGCCTGAACGGATCGTGGGCGAGCTG
AACGGCGGCTGGCGGGTCGCGATGCACATCCTCGCCCACGAGCGTGGGACGTTCGCGTGGTTCCGGCACAGTTTTCTGTA
CCGCCAGATGTTCGACAACATCGCGCACGGCTCGCCGGCCGGCGACGCCCTTCTCGGGCACGCTCTGCTCGACCTGGCCG
CGGTCCGGGCCAGCAGCCACGCGGCGTTGCGGGCCCACGCTGCCGGCACCACGCTCGGCCCCAGGGCCGCGTTCGTGAAG
CTGCTGCTGGCAGCCAGCGAGCAGGCGGTCAACGACTGGGTGCTCGCGAGTGATCCGGACCTCGCCGTCGGGATACAGGA
CGACCACGCGGCGGTGTGCCGACAGGACTACCTGTTCTCCCGCATCGTCACCGTCTACGGAGGCTCCCAGCAGATGCAGC
TCGACACCATCGCCAAGCAGATCCTGCGTCTGCCGTGA

Upstream 100 bases:

>100_bases
AGCGGGCTACTCGGGTCCTCGCGCTCGACCACGTCACTCCCTGAGGGTTCCACGAACTTCGACGTTGCTTCGAGGTTCGC
ATGTTCCTATATTGACGTCA

Downstream 100 bases:

>100_bases
CGCCGGTTGCGGAGCAGGACGACCGGGAGTTCCTCGATGCGGCACGCCGCGCGCTGTGCGCCTCCGGCAGCGCCGACCCG
CTCGCTTCGCTGGGGTGGTG

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA reductase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLHITASSERDPLRDDACAYAKEFEAWLTSRPPTLDAAARPIASYEKRVAVTSELMRVLFDEGWARYGWPEEAGGFGGDI
LHRAAMWEALARHGLPAMALFEHLEILAPTLVAMGRPDFIAQVLPRFLRGEQRWSQGFSEPDAGSDLASARTRAVAADGV
HVITGRKIWTSWASYATWCLVLARTGTLESRHRGLTAFAVDLRAPGVEVRPIEQANGTDELAEVTFDEVPVGPERIVGEL
NGGWRVAMHILAHERGTFAWFRHSFLYRQMFDNIAHGSPAGDALLGHALLDLAAVRASSHAALRAHAAGTTLGPRAAFVK
LLLAASEQAVNDWVLASDPDLAVGIQDDHAAVCRQDYLFSRIVTVYGGSQQMQLDTIAKQILRLP

Sequences:

>Translated_385_residues
MLHITASSERDPLRDDACAYAKEFEAWLTSRPPTLDAAARPIASYEKRVAVTSELMRVLFDEGWARYGWPEEAGGFGGDI
LHRAAMWEALARHGLPAMALFEHLEILAPTLVAMGRPDFIAQVLPRFLRGEQRWSQGFSEPDAGSDLASARTRAVAADGV
HVITGRKIWTSWASYATWCLVLARTGTLESRHRGLTAFAVDLRAPGVEVRPIEQANGTDELAEVTFDEVPVGPERIVGEL
NGGWRVAMHILAHERGTFAWFRHSFLYRQMFDNIAHGSPAGDALLGHALLDLAAVRASSHAALRAHAAGTTLGPRAAFVK
LLLAASEQAVNDWVLASDPDLAVGIQDDHAAVCRQDYLFSRIVTVYGGSQQMQLDTIAKQILRLP
>Mature_385_residues
MLHITASSERDPLRDDACAYAKEFEAWLTSRPPTLDAAARPIASYEKRVAVTSELMRVLFDEGWARYGWPEEAGGFGGDI
LHRAAMWEALARHGLPAMALFEHLEILAPTLVAMGRPDFIAQVLPRFLRGEQRWSQGFSEPDAGSDLASARTRAVAADGV
HVITGRKIWTSWASYATWCLVLARTGTLESRHRGLTAFAVDLRAPGVEVRPIEQANGTDELAEVTFDEVPVGPERIVGEL
NGGWRVAMHILAHERGTFAWFRHSFLYRQMFDNIAHGSPAGDALLGHALLDLAAVRASSHAALRAHAAGTTLGPRAAFVK
LLLAASEQAVNDWVLASDPDLAVGIQDDHAAVCRQDYLFSRIVTVYGGSQQMQLDTIAKQILRLP

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma- butyrobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=355, Percent_Identity=25.6338028169014, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI21361497, Length=193, Percent_Identity=30.5699481865285, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4557235, Length=195, Percent_Identity=31.7948717948718, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI76496475, Length=195, Percent_Identity=31.7948717948718, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI187960098, Length=253, Percent_Identity=23.3201581027668, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI4557231, Length=253, Percent_Identity=23.3201581027668, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI4501857, Length=364, Percent_Identity=22.8021978021978, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786223, Length=344, Percent_Identity=25.8720930232558, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081958, Length=346, Percent_Identity=26.0115606936416, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17508101, Length=336, Percent_Identity=26.4880952380952, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17570075, Length=360, Percent_Identity=24.7222222222222, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17538396, Length=202, Percent_Identity=28.2178217821782, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17534899, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71985184, Length=219, Percent_Identity=30.5936073059361, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71985192, Length=219, Percent_Identity=30.5936073059361, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17569725, Length=221, Percent_Identity=26.2443438914027, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17506239, Length=369, Percent_Identity=25.2032520325203, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17505929, Length=205, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24666513, Length=349, Percent_Identity=29.2263610315186, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281363737, Length=196, Percent_Identity=31.1224489795918, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21355753, Length=192, Percent_Identity=31.25, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42147; Mature: 42147

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHITASSERDPLRDDACAYAKEFEAWLTSRPPTLDAAARPIASYEKRVAVTSELMRVLF
CEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DEGWARYGWPEEAGGFGGDILHRAAMWEALARHGLPAMALFEHLEILAPTLVAMGRPDFI
HCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
AQVLPRFLRGEQRWSQGFSEPDAGSDLASARTRAVAADGVHVITGRKIWTSWASYATWCL
HHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
VLARTGTLESRHRGLTAFAVDLRAPGVEVRPIEQANGTDELAEVTFDEVPVGPERIVGEL
HHHHCCCHHHHHCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHC
NGGWRVAMHILAHERGTFAWFRHSFLYRQMFDNIAHGSPAGDALLGHALLDLAAVRASSH
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
AALRAHAAGTTLGPRAAFVKLLLAASEQAVNDWVLASDPDLAVGIQDDHAAVCRQDYLFS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHH
RIVTVYGGSQQMQLDTIAKQILRLP
HHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHITASSERDPLRDDACAYAKEFEAWLTSRPPTLDAAARPIASYEKRVAVTSELMRVLF
CEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DEGWARYGWPEEAGGFGGDILHRAAMWEALARHGLPAMALFEHLEILAPTLVAMGRPDFI
HCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
AQVLPRFLRGEQRWSQGFSEPDAGSDLASARTRAVAADGVHVITGRKIWTSWASYATWCL
HHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
VLARTGTLESRHRGLTAFAVDLRAPGVEVRPIEQANGTDELAEVTFDEVPVGPERIVGEL
HHHHCCCHHHHHCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHC
NGGWRVAMHILAHERGTFAWFRHSFLYRQMFDNIAHGSPAGDALLGHALLDLAAVRASSH
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
AALRAHAAGTTLGPRAAFVKLLLAASEQAVNDWVLASDPDLAVGIQDDHAAVCRQDYLFS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHH
RIVTVYGGSQQMQLDTIAKQILRLP
HHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA