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The map label for this gene is hypF [H]

Identifier: 158314311

GI number: 158314311

Start: 2959567

End: 2961774

Strand: Direct

Name: hypF [H]

Synonym: Franean1_2481

Alternate gene names: 158314311

Gene position: 2959567-2961774 (Clockwise)

Preceding gene: 158314310

Following gene: 158314325

Centisome position: 32.95

GC content: 75.23

Gene sequence:

>2208_bases
GTGGACGTCTCCGGCGGTCCCGCGCCGCCCGGAGCCGGGCCGGCGGCGCCGGGGTTCCGGATCGCCCCCAGCGACCCGGC
CCGCGGCGGGGCCGGGCCGCGGACGCGGGTCTGCGCGGACAGTGCCACCTGTGCGGACTGCCTGGCCGAGCTCGCCGACC
CGGCCGACCGGCGCTACCGCTACCCGTTCATCAACTGCACCAACTGCGGCCCCCGCTTCACGATCATCCGGGACGTCCCC
TACGACCGGCCGGCCACGACGATGGCCGGATTCCGGATGTGCGCGCGGTGCCGGGCGGAGTACGACGACCCGGCCGACCG
CCGCTTCCACGCCCAGCCGGTGTGCTGTCCGGACTGCGGCCCCACCCTCAGCTTCCGGCCCACCGCCGCCGCGTCGCCGA
CGTCCGACGAGCCGCTGGAGCGAGCAGCGGGGCTGCTGCGGGGCGGCGGGATTCTCGCGGTGAAGGGGCTGGGCGGGTAC
CACCTGGCCGTGCTCGCCGCGGACGGGTCCGCGGTGGCGACCCTGCGCCGGCGCAAGCACCGGGACGCCAAGCCGTTCGC
CGTCATGGTGCGCGACCTGGCGACCGTCGAGGAGATGTGCTGCCTCGACCCCACCGCGCGCCTCCTGCTCACCGGGCGGC
GCCGGCCGATCGTCCTGGTCGAGCGCTGCCGGCCCGCTAGGCGGGTCGCCGAGGCGGTGGCGCCGGGCACCGGGACGCTC
GGCGTGATGCTGCCGTACACGCCGCTGCACCACCTTCTGCTCGGCCTGCTGCCCGGCCCGATCGTGCTGACCAGCGGCAA
CATCTCCAACGAGCCGATCGTGTACCACGACGAGGAGGCCCTCGCCGCGCTCAGCCCGGTCGCGGACGGTTTCCTCGCCC
ACGACCGGGCCATCCACACCAGGCTCGACGACTCGGTCGCCCGGTCGGTGCGCGGCCGGGAGACGCTCGTGCGCCGCTCC
CGCGGCTACGCCCCGGAGCCGCTGCCGCTGCCCTGGGACGTCCAGCGGCCGGTGCTGGCCTGCGGGGCCGAGCTGAAGAA
CACCGTGTGCCTGGCGGCGGGGCGGCGGGCGTACCTCTCCGGCCACATCGGGGACCTGGAGAACCACGAGACGCTGCGGT
CGTTCGTCAGCGGGATCGACCACCTGCGCCGGCTGTTCGACATCTCGCCCGAGCTGGTAGCGCACGACCTGCACCCGGAG
TATCTGTCCACGAAGTGGGCGCTGGAGCAGGACCTGCCGGTCGTCGGCGTGCAGCATCACCACGCGCACATTGCCTCCTG
CCTGGCGGACAACGGCCATCCCGGGCCGGTGCTCGGTGTGGCCTTCGACGGCCTCGGCTTCGGTCCGGACGGCACCCTGT
GGGGCGGGGAGTTCCTGTGGGCCGACCTGGCCGGCTTCACCCGGCTCGCACATCTCACGCCCGTCCCGATGCCCGGGGGC
GTCGCCGCGATCCGGGCACCATGGCGGATGGCCGCCGCCCACCTGCTCGCCGCCGGCGTCGCCGACCCGGACCGCCTCGC
GGTCGCCCGCCGCAACGCCGACCGCTGGGACGCGGTCGCCGCCCTGGCCAGGTCCGGTGCCGCCCCGTCCCCGGTGGGTG
CCCCGCTGACCAGCAGCGTCGGACGGCTGTTCGACGCCGTCGCGGCTCTCGTCGGCGTCCGGGACACGATCACCTACGAA
GGGCAGGCCGCGATCGAGCTGGAGCGGATCGCCGCCCCCGGCGACTACGGCCGCTACCCGGCGAGCCCCCCGCCGTCGGG
TGCCGGGCTGCTGCTGCCGGGCGCCGACCTGATCCGCGGCGTCGTGGACGACCTGCGCGAACGCGTCGACCCCACGGTGA
TCGCCGCCCGGTTCCACAGCACGCTGGCCGGGCTCACCGTCGACATCTGTGCCGCCCTGCGTGCGGGGCTCCCGGGGCGC
CAGGCGGGAGCGGTGGCGCTCTCCGGCGGCGTCTTCGGCAACCTGCGGCTGCTCGGCGAGATCATGGACGGGCTGGTAGA
CCGGGGCTTCACCGTCCTGACCCACTCCCGGGTGCCCTGCAACGACGGCGGGATCAGCCTCGGTCAGGCGGTGGTCGCGA
ACGCCCGCAGCCAGCCGTACCAGTCGTCGAGCCCGGTGCCGGCGATCGCCGACACCGGCAGCACCGGGGCGGACGGGTTG
ACCAGCCGCACGGACTCGGCGAACAGGTCACCGTCGAAGGCGAGGTAG

Upstream 100 bases:

>100_bases
TGAGGGCGTCCACATAGCGCTGGACGGCGCAGGCCCGGAGGTCGCCGCGTTCCTGACCGCGCTGCGCGAGGAGGCGCCGC
CGCTCGCCCAGATCGACAGC

Downstream 100 bases:

>100_bases
GGCAGCAGGTCGACCTTGGTGAGCAGAACGAGATCGGCGACCCGGTACATGTGCGGGTACTTGCTGGGCTTGTCCTCACC
CTCGGTGACCGAGGCCAGCA

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MDVSGGPAPPGAGPAAPGFRIAPSDPARGGAGPRTRVCADSATCADCLAELADPADRRYRYPFINCTNCGPRFTIIRDVP
YDRPATTMAGFRMCARCRAEYDDPADRRFHAQPVCCPDCGPTLSFRPTAAASPTSDEPLERAAGLLRGGGILAVKGLGGY
HLAVLAADGSAVATLRRRKHRDAKPFAVMVRDLATVEEMCCLDPTARLLLTGRRRPIVLVERCRPARRVAEAVAPGTGTL
GVMLPYTPLHHLLLGLLPGPIVLTSGNISNEPIVYHDEEALAALSPVADGFLAHDRAIHTRLDDSVARSVRGRETLVRRS
RGYAPEPLPLPWDVQRPVLACGAELKNTVCLAAGRRAYLSGHIGDLENHETLRSFVSGIDHLRRLFDISPELVAHDLHPE
YLSTKWALEQDLPVVGVQHHHAHIASCLADNGHPGPVLGVAFDGLGFGPDGTLWGGEFLWADLAGFTRLAHLTPVPMPGG
VAAIRAPWRMAAAHLLAAGVADPDRLAVARRNADRWDAVAALARSGAAPSPVGAPLTSSVGRLFDAVAALVGVRDTITYE
GQAAIELERIAAPGDYGRYPASPPPSGAGLLLPGADLIRGVVDDLRERVDPTVIAARFHSTLAGLTVDICAALRAGLPGR
QAGAVALSGGVFGNLRLLGEIMDGLVDRGFTVLTHSRVPCNDGGISLGQAVVANARSQPYQSSSPVPAIADTGSTGADGL
TSRTDSANRSPSKAR

Sequences:

>Translated_735_residues
MDVSGGPAPPGAGPAAPGFRIAPSDPARGGAGPRTRVCADSATCADCLAELADPADRRYRYPFINCTNCGPRFTIIRDVP
YDRPATTMAGFRMCARCRAEYDDPADRRFHAQPVCCPDCGPTLSFRPTAAASPTSDEPLERAAGLLRGGGILAVKGLGGY
HLAVLAADGSAVATLRRRKHRDAKPFAVMVRDLATVEEMCCLDPTARLLLTGRRRPIVLVERCRPARRVAEAVAPGTGTL
GVMLPYTPLHHLLLGLLPGPIVLTSGNISNEPIVYHDEEALAALSPVADGFLAHDRAIHTRLDDSVARSVRGRETLVRRS
RGYAPEPLPLPWDVQRPVLACGAELKNTVCLAAGRRAYLSGHIGDLENHETLRSFVSGIDHLRRLFDISPELVAHDLHPE
YLSTKWALEQDLPVVGVQHHHAHIASCLADNGHPGPVLGVAFDGLGFGPDGTLWGGEFLWADLAGFTRLAHLTPVPMPGG
VAAIRAPWRMAAAHLLAAGVADPDRLAVARRNADRWDAVAALARSGAAPSPVGAPLTSSVGRLFDAVAALVGVRDTITYE
GQAAIELERIAAPGDYGRYPASPPPSGAGLLLPGADLIRGVVDDLRERVDPTVIAARFHSTLAGLTVDICAALRAGLPGR
QAGAVALSGGVFGNLRLLGEIMDGLVDRGFTVLTHSRVPCNDGGISLGQAVVANARSQPYQSSSPVPAIADTGSTGADGL
TSRTDSANRSPSKAR
>Mature_735_residues
MDVSGGPAPPGAGPAAPGFRIAPSDPARGGAGPRTRVCADSATCADCLAELADPADRRYRYPFINCTNCGPRFTIIRDVP
YDRPATTMAGFRMCARCRAEYDDPADRRFHAQPVCCPDCGPTLSFRPTAAASPTSDEPLERAAGLLRGGGILAVKGLGGY
HLAVLAADGSAVATLRRRKHRDAKPFAVMVRDLATVEEMCCLDPTARLLLTGRRRPIVLVERCRPARRVAEAVAPGTGTL
GVMLPYTPLHHLLLGLLPGPIVLTSGNISNEPIVYHDEEALAALSPVADGFLAHDRAIHTRLDDSVARSVRGRETLVRRS
RGYAPEPLPLPWDVQRPVLACGAELKNTVCLAAGRRAYLSGHIGDLENHETLRSFVSGIDHLRRLFDISPELVAHDLHPE
YLSTKWALEQDLPVVGVQHHHAHIASCLADNGHPGPVLGVAFDGLGFGPDGTLWGGEFLWADLAGFTRLAHLTPVPMPGG
VAAIRAPWRMAAAHLLAAGVADPDRLAVARRNADRWDAVAALARSGAAPSPVGAPLTSSVGRLFDAVAALVGVRDTITYE
GQAAIELERIAAPGDYGRYPASPPPSGAGLLLPGADLIRGVVDDLRERVDPTVIAARFHSTLAGLTVDICAALRAGLPGR
QAGAVALSGGVFGNLRLLGEIMDGLVDRGFTVLTHSRVPCNDGGISLGQAVVANARSQPYQSSSPVPAIADTGSTGADGL
TSRTDSANRSPSKAR

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=673, Percent_Identity=42.9420505200594, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 77321; Mature: 77321

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSGGPAPPGAGPAAPGFRIAPSDPARGGAGPRTRVCADSATCADCLAELADPADRRYR
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCHHCCCC
YPFINCTNCGPRFTIIRDVPYDRPATTMAGFRMCARCRAEYDDPADRRFHAQPVCCPDCG
CCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PTLSFRPTAAASPTSDEPLERAAGLLRGGGILAVKGLGGYHLAVLAADGSAVATLRRRKH
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCHHHHHHHHHHC
RDAKPFAVMVRDLATVEEMCCLDPTARLLLTGRRRPIVLVERCRPARRVAEAVAPGTGTL
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEHHCCCHHHHHHHHHCCCCCCE
GVMLPYTPLHHLLLGLLPGPIVLTSGNISNEPIVYHDEEALAALSPVADGFLAHDRAIHT
EEEECCCHHHHHHHHHCCCCEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
RLDDSVARSVRGRETLVRRSRGYAPEPLPLPWDVQRPVLACGAELKNTVCLAAGRRAYLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCHHHHCCEEECCCCHHEEC
GHIGDLENHETLRSFVSGIDHLRRLFDISPELVAHDLHPEYLSTKWALEQDLPVVGVQHH
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCEEECCHH
HAHIASCLADNGHPGPVLGVAFDGLGFGPDGTLWGGEFLWADLAGFTRLAHLTPVPMPGG
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
VAAIRAPWRMAAAHLLAAGVADPDRLAVARRNADRWDAVAALARSGAAPSPVGAPLTSSV
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
GRLFDAVAALVGVRDTITYEGQAAIELERIAAPGDYGRYPASPPPSGAGLLLPGADLIRG
HHHHHHHHHHHCCHHHEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHH
VVDDLRERVDPTVIAARFHSTLAGLTVDICAALRAGLPGRQAGAVALSGGVFGNLRLLGE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHH
IMDGLVDRGFTVLTHSRVPCNDGGISLGQAVVANARSQPYQSSSPVPAIADTGSTGADGL
HHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCC
TSRTDSANRSPSKAR
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MDVSGGPAPPGAGPAAPGFRIAPSDPARGGAGPRTRVCADSATCADCLAELADPADRRYR
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCHHCCCC
YPFINCTNCGPRFTIIRDVPYDRPATTMAGFRMCARCRAEYDDPADRRFHAQPVCCPDCG
CCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PTLSFRPTAAASPTSDEPLERAAGLLRGGGILAVKGLGGYHLAVLAADGSAVATLRRRKH
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCHHHHHHHHHHC
RDAKPFAVMVRDLATVEEMCCLDPTARLLLTGRRRPIVLVERCRPARRVAEAVAPGTGTL
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEHHCCCHHHHHHHHHCCCCCCE
GVMLPYTPLHHLLLGLLPGPIVLTSGNISNEPIVYHDEEALAALSPVADGFLAHDRAIHT
EEEECCCHHHHHHHHHCCCCEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
RLDDSVARSVRGRETLVRRSRGYAPEPLPLPWDVQRPVLACGAELKNTVCLAAGRRAYLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCHHHHCCEEECCCCHHEEC
GHIGDLENHETLRSFVSGIDHLRRLFDISPELVAHDLHPEYLSTKWALEQDLPVVGVQHH
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCEEECCHH
HAHIASCLADNGHPGPVLGVAFDGLGFGPDGTLWGGEFLWADLAGFTRLAHLTPVPMPGG
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
VAAIRAPWRMAAAHLLAAGVADPDRLAVARRNADRWDAVAALARSGAAPSPVGAPLTSSV
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
GRLFDAVAALVGVRDTITYEGQAAIELERIAAPGDYGRYPASPPPSGAGLLLPGADLIRG
HHHHHHHHHHHCCHHHEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHH
VVDDLRERVDPTVIAARFHSTLAGLTVDICAALRAGLPGRQAGAVALSGGVFGNLRLLGE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHH
IMDGLVDRGFTVLTHSRVPCNDGGISLGQAVVANARSQPYQSSSPVPAIADTGSTGADGL
HHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCC
TSRTDSANRSPSKAR
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]