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The map label for this gene is rutD [H]

Identifier: 158313735

GI number: 158313735

Start: 2280108

End: 2280923

Strand: Direct

Name: rutD [H]

Synonym: Franean1_1900

Alternate gene names: 158313735

Gene position: 2280108-2280923 (Clockwise)

Preceding gene: 158313734

Following gene: 158313743

Centisome position: 25.39

GC content: 74.88

Gene sequence:

>816_bases
GTGTCGACCATCGATGTCCGGGGCGTGTCGATCGCTTATGGAGATACCGGTTCCCCAGCAGGTCGGCCGGATGCGCCGAC
GATTGTGTTCGGGCACGGCCTGCTGTTCGGCGGCTGGGTGTTCCGCGCGCAGGTCGCGGCGCTGCGCGAGCACTACCGCT
GCGTCACCCTTGACTGGCGCGGCCAGGGTGACACACCGCCGACCCCCGGCGGGTACGACATGGACACCCTCACCGCCGAC
GCGGTCGCCGTGATCCAGCGGCTCGGCCTCGCGCCCGTGCACTGGGTGGGACTGTCCATGGGCGGCTTCGTCGGCCTGCG
GATCGCGGCCCGCCACGGCGAGCTGCTGCGGTCGCTGACGCTGCTCGACTCGAGCGCCGATGCCGAGGAGCCGACCGAGG
CCGGTGCCTACCGGCGCCTGGCCCTGGTGGCTCGGCTGGTCGGCGTCCGGCCGGTCCTCGGCCGGGTCCGTCCGACCATG
TTCGGCCCCGCCTTCCTCGCGGACCCGGACGGCCGGCCCGTCGTCGCGGAGTGGGCCGGCCGGCTCGACCGGGTTGACCG
CTCCGCCCTGCGCAAGGCGGTGCTCGGGGTGGCCGACCGCCCGCCCGTCGACCACGAGATCCCCGGCATCGCGGTGCCCA
CGCTCGTCGTCGTCGGCGCCGACGACGTGGCCACCCCGCCCGAACGGGCCGAGCGGATGGCGGCCCGTATCCCCGGCGCC
CGGCTCGAGGTTGTCGCCGACTGCGGCCACACCAGCCCGCTGGAGCAGCCCGACGTCATCACCGGCCTGCTGCGCGGGTT
CGTCGCCGGGGCGTGA

Upstream 100 bases:

>100_bases
TAACTGAATGTTTTCCGGATGTCGTGGAGAATATCGGGGCGGGTAGTCGAGGTGCCCGCTTCCTGGCATGGGATAAGATC
TCAAGAAGGAGCGGAGCGCT

Downstream 100 bases:

>100_bases
TCCCACCGTTCCAGGGCGCCGCCGGCCCGTGACGCCGGTGGCGGCCCGTGACGCCGGTGGCGGCCCGGACGGCCGCCGCC
TCCCGAGACGCCGTTACCGG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSTIDVRGVSIAYGDTGSPAGRPDAPTIVFGHGLLFGGWVFRAQVAALREHYRCVTLDWRGQGDTPPTPGGYDMDTLTAD
AVAVIQRLGLAPVHWVGLSMGGFVGLRIAARHGELLRSLTLLDSSADAEEPTEAGAYRRLALVARLVGVRPVLGRVRPTM
FGPAFLADPDGRPVVAEWAGRLDRVDRSALRKAVLGVADRPPVDHEIPGIAVPTLVVVGADDVATPPERAERMAARIPGA
RLEVVADCGHTSPLEQPDVITGLLRGFVAGA

Sequences:

>Translated_271_residues
MSTIDVRGVSIAYGDTGSPAGRPDAPTIVFGHGLLFGGWVFRAQVAALREHYRCVTLDWRGQGDTPPTPGGYDMDTLTAD
AVAVIQRLGLAPVHWVGLSMGGFVGLRIAARHGELLRSLTLLDSSADAEEPTEAGAYRRLALVARLVGVRPVLGRVRPTM
FGPAFLADPDGRPVVAEWAGRLDRVDRSALRKAVLGVADRPPVDHEIPGIAVPTLVVVGADDVATPPERAERMAARIPGA
RLEVVADCGHTSPLEQPDVITGLLRGFVAGA
>Mature_270_residues
STIDVRGVSIAYGDTGSPAGRPDAPTIVFGHGLLFGGWVFRAQVAALREHYRCVTLDWRGQGDTPPTPGGYDMDTLTADA
VAVIQRLGLAPVHWVGLSMGGFVGLRIAARHGELLRSLTLLDSSADAEEPTEAGAYRRLALVARLVGVRPVLGRVRPTMF
GPAFLADPDGRPVVAEWAGRLDRVDRSALRKAVLGVADRPPVDHEIPGIAVPTLVVVGADDVATPPERAERMAARIPGAR
LEVVADCGHTSPLEQPDVITGLLRGFVAGA

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=264, Percent_Identity=25.3787878787879, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI23200008, Length=264, Percent_Identity=25.3787878787879, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 28608; Mature: 28477

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIDVRGVSIAYGDTGSPAGRPDAPTIVFGHGLLFGGWVFRAQVAALREHYRCVTLDWR
CCCEEECEEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEEC
GQGDTPPTPGGYDMDTLTADAVAVIQRLGLAPVHWVGLSMGGFVGLRIAARHGELLRSLT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHH
LLDSSADAEEPTEAGAYRRLALVARLVGVRPVLGRVRPTMFGPAFLADPDGRPVVAEWAG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCEEHHHHHH
RLDRVDRSALRKAVLGVADRPPVDHEIPGIAVPTLVVVGADDVATPPERAERMAARIPGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECEEEEECCCCCCCCHHHHHHHHHHCCCC
RLEVVADCGHTSPLEQPDVITGLLRGFVAGA
EEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STIDVRGVSIAYGDTGSPAGRPDAPTIVFGHGLLFGGWVFRAQVAALREHYRCVTLDWR
CCEEECEEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEEC
GQGDTPPTPGGYDMDTLTADAVAVIQRLGLAPVHWVGLSMGGFVGLRIAARHGELLRSLT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHH
LLDSSADAEEPTEAGAYRRLALVARLVGVRPVLGRVRPTMFGPAFLADPDGRPVVAEWAG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCEEHHHHHH
RLDRVDRSALRKAVLGVADRPPVDHEIPGIAVPTLVVVGADDVATPPERAERMAARIPGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECEEEEECCCCCCCCHHHHHHHHHHCCCC
RLEVVADCGHTSPLEQPDVITGLLRGFVAGA
EEEEEEECCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA