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The map label for this gene is regA [H]

Identifier: 158313716

GI number: 158313716

Start: 2259119

End: 2260126

Strand: Direct

Name: regA [H]

Synonym: Franean1_1880

Alternate gene names: 158313716

Gene position: 2259119-2260126 (Clockwise)

Preceding gene: 158313715

Following gene: 158313718

Centisome position: 25.15

GC content: 71.13

Gene sequence:

>1008_bases
ATGGCGAAGCGAGTCACCATCGGTGACGTCGCCGCGCACGCCGGCGTCTCCACGGCGACGGTCTCCCGCGTGCTCAACGG
GGGCGCCGTCACCGAGGCGACCGCGACCCGGGTGCGGGAGGCGGTCACCGCGCTGGAGTACAGCCCCAACGCGCTCACCA
GAAGTATCTTCGCCGGTCGCTCCAGCACGATCGGTGTTGTCATCCGTGACCTGAGCAGCCCCTTCTACCTCGACCTGATC
CGTGGCATCGACGACGTGGCCGCCGCCAACGACAGCCTCGTCATGCTCGCCAACACGTTCCGCCAGGCCGACCATGAAAT
CGCGCTCGTCAGGATGATGGACGAGCACCGGGTGCGCGGCCTGATCGTGACCGCCGGCGAGCAGACCGACAGCCGCACCC
GCCGCATGGCCGAGACCGGCACGCCGTGCGTGATCGTCGCGCGTACCGTGCGGGACCCTCCGCCCGACCTGCATGCCGTC
AGCCTCGACAACGTCGGGGCCGGTCGTCTGATGGCCAGGCATCTGGTCGGGTGCGGGCGGTCGTCCGTCGGGGTCGTCAC
CTCCGGGAGCCGCCCGTCCCAGCTCGAGCGCACGGCGGGGCTGCGGAAGGCGCTCATCGAGCTCGGACGTCCGCTGCCCG
CGGACGCGGTGGCCGTCGCCGAGACGGCGGAAGAGGCCGACCATGCTGTCGGCACGCTCCTCGCGCGCGCACATGCTCGC
GGAACGCCGTTGGACGCGGTCGCCTGCGCCACGGGCCGCCTGACCGTCGCCGCGTACTCGGCGCTCACGGCTCGCGGCCT
CGCCATTCCCGACGACATCGCGTTCGTGACGATGGACGACTTTCCCTGGGCCACCGCGCTCGGGATCACCGTGATCACCC
AGCCCTCGTACGAGATGGGCCGGCGGGCAGCCGAGCTCGTCGTCGAACGCCCCGGTGAAGCGGTCAGCATCGTCTTCGAG
CCGATGCTGGTCGCCCGCGGCTCCGCCGGCGAGAGAAACAGTCCGTGA

Upstream 100 bases:

>100_bases
GACCGGGACGTCCGCCGGCAGGGCACCCGGCCGGAGCGTCGGCCGACCAGACGCCCTGACCGGGCACCGCCGGAACCGTT
CGGAGGGACGCGCAGCGACC

Downstream 100 bases:

>100_bases
CCTGAGCGGCAGCGGTCAGTGTTCCGGGACGCCTCGGTCAGGGCCGCCGGGCGGGCTCGTCACGAGCCGGTGGGGTCCAG
GCGGCCCGCGCCGTCGTGCG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAKRVTIGDVAAHAGVSTATVSRVLNGGAVTEATATRVREAVTALEYSPNALTRSIFAGRSSTIGVVIRDLSSPFYLDLI
RGIDDVAAANDSLVMLANTFRQADHEIALVRMMDEHRVRGLIVTAGEQTDSRTRRMAETGTPCVIVARTVRDPPPDLHAV
SLDNVGAGRLMARHLVGCGRSSVGVVTSGSRPSQLERTAGLRKALIELGRPLPADAVAVAETAEEADHAVGTLLARAHAR
GTPLDAVACATGRLTVAAYSALTARGLAIPDDIAFVTMDDFPWATALGITVITQPSYEMGRRAAELVVERPGEAVSIVFE
PMLVARGSAGERNSP

Sequences:

>Translated_335_residues
MAKRVTIGDVAAHAGVSTATVSRVLNGGAVTEATATRVREAVTALEYSPNALTRSIFAGRSSTIGVVIRDLSSPFYLDLI
RGIDDVAAANDSLVMLANTFRQADHEIALVRMMDEHRVRGLIVTAGEQTDSRTRRMAETGTPCVIVARTVRDPPPDLHAV
SLDNVGAGRLMARHLVGCGRSSVGVVTSGSRPSQLERTAGLRKALIELGRPLPADAVAVAETAEEADHAVGTLLARAHAR
GTPLDAVACATGRLTVAAYSALTARGLAIPDDIAFVTMDDFPWATALGITVITQPSYEMGRRAAELVVERPGEAVSIVFE
PMLVARGSAGERNSP
>Mature_334_residues
AKRVTIGDVAAHAGVSTATVSRVLNGGAVTEATATRVREAVTALEYSPNALTRSIFAGRSSTIGVVIRDLSSPFYLDLIR
GIDDVAAANDSLVMLANTFRQADHEIALVRMMDEHRVRGLIVTAGEQTDSRTRRMAETGTPCVIVARTVRDPPPDLHAVS
LDNVGAGRLMARHLVGCGRSSVGVVTSGSRPSQLERTAGLRKALIELGRPLPADAVAVAETAEEADHAVGTLLARAHARG
TPLDAVACATGRLTVAAYSALTARGLAIPDDIAFVTMDDFPWATALGITVITQPSYEMGRRAAELVVERPGEAVSIVFEP
MLVARGSAGERNSP

Specific function: Involved in the regulation of amylase production [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=30.8823529411765, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=28.8288288288288, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=28.6144578313253, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=29.7619047619048, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787906, Length=320, Percent_Identity=29.6875, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=27.7777777777778, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=27.8996865203762, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789068, Length=302, Percent_Identity=26.4900662251656, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=22.3642172523962, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=24.1379310344828, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35194; Mature: 35062

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRVTIGDVAAHAGVSTATVSRVLNGGAVTEATATRVREAVTALEYSPNALTRSIFAGR
CCCEEEHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
SSTIGVVIRDLSSPFYLDLIRGIDDVAAANDSLVMLANTFRQADHEIALVRMMDEHRVRG
CCEEEEEEECCCCCHHHHHHHCCHHHHCCCCCEEEEEHHHHHCCCCEEEEEEHHHCCCCE
LIVTAGEQTDSRTRRMAETGTPCVIVARTVRDPPPDLHAVSLDNVGAGRLMARHLVGCGR
EEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
SSVGVVTSGSRPSQLERTAGLRKALIELGRPLPADAVAVAETAEEADHAVGTLLARAHAR
CCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GTPLDAVACATGRLTVAAYSALTARGLAIPDDIAFVTMDDFPWATALGITVITQPSYEMG
CCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHCEEEEECCCHHHH
RRAAELVVERPGEAVSIVFEPMLVARGSAGERNSP
HHHHHHHHHCCCCEEEEEEHHHHEECCCCCCCCCC
>Mature Secondary Structure 
AKRVTIGDVAAHAGVSTATVSRVLNGGAVTEATATRVREAVTALEYSPNALTRSIFAGR
CCEEEHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
SSTIGVVIRDLSSPFYLDLIRGIDDVAAANDSLVMLANTFRQADHEIALVRMMDEHRVRG
CCEEEEEEECCCCCHHHHHHHCCHHHHCCCCCEEEEEHHHHHCCCCEEEEEEHHHCCCCE
LIVTAGEQTDSRTRRMAETGTPCVIVARTVRDPPPDLHAVSLDNVGAGRLMARHLVGCGR
EEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
SSVGVVTSGSRPSQLERTAGLRKALIELGRPLPADAVAVAETAEEADHAVGTLLARAHAR
CCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GTPLDAVACATGRLTVAAYSALTARGLAIPDDIAFVTMDDFPWATALGITVITQPSYEMG
CCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHCEEEEECCCHHHH
RRAAELVVERPGEAVSIVFEPMLVARGSAGERNSP
HHHHHHHHHCCCCEEEEEEHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]