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The map label for this gene is pfkA1 [H]

Identifier: 158313668

GI number: 158313668

Start: 2198057

End: 2199082

Strand: Direct

Name: pfkA1 [H]

Synonym: Franean1_1832

Alternate gene names: 158313668

Gene position: 2198057-2199082 (Clockwise)

Preceding gene: 158313667

Following gene: 158313669

Centisome position: 24.47

GC content: 71.54

Gene sequence:

>1026_bases
GTGAAGATCGGTGTGCTGACCGGCGGTGGCGACTGTCCTGGTCTGAACGCGGTGATCCGTGCCGTCGTACGCAAGGGTGT
CGGCGTCTACGGGCACAGCTTCGTCGGGTTCCGGGACGGCTGGCGCGGACCACTGGAGAACGACACCGTCCCGTTGGACG
TGACGGCCGTGCGCGGCATCCTGCCCCGCGGCGGGACCATCCTCGGTTCCAGCCGGACGAATCCGTTCGCCTCGGCGGAC
GGTCCGTCCCGCATCCGGGAGACCCTTGACCGCACGGGCGTGGACGCCATCGTCGCCGTCGGCGGCGAGGACACCCTCGG
CGCCGCTGCGCGCCTGCACGCCGAGGGCATCCCGGTCGTCGGGGTGCCGAAGACGATCGACAACGATCTCTCCGCGACCG
ACTACACGTTCGGCTTCGACACCGCGGTGAACATCGCGATGGAGGCCATCGACCGCCTGCACACGACGGCGGAGAGCCAC
CACCGCGTCCTCGTCGTCGAGGTGATGGGCCGCCACGCCGGGTGGATCGCCCTGCACAGCGGGATGGCGGCTGGCGCGAA
CGTCATCCTCATCCCCGAGCGGCCCTTCGACATCGACAAGGTCTGCGCCTTCGTCGAGCAGCGCTTCGCCACCCGCTACG
CGCCGATCATCGTGGTGGCCGAGGGCGCCCGGCCGATCGCCGGCACCGCCATGGCCAGGGAGGGCGAGCTGGACGCCTTC
GGTCACGTGCGTCTGCAGGGCATCTCCGGCCTGCTCGAGACCGAGATCCGCGGCCGGACGGGCCGGGAGACCCGGGCGAC
GGTGCTCGGCCACGTCCAGCGCGGCGGGACGCCGACCGCCTTCGACCGCTGGCTGGCCACCCGGTTCGGGCTGCACGCCG
TCGACGCCGTGCACGAGGGCGACTTCGGGACGATGGTCGCCCTGCACGGCACGCGGATCGACCGGGTTCCGCTCAGCGAG
GCGACCGGCGAGCTCAAGACCGTCCCCGAGCACCTCTACGGCGAGGCGGAGGTCTTCTTCGGCTGA

Upstream 100 bases:

>100_bases
TGTTTACGGTGAGGGGCTGGTCGGGGTCTCCGGGGAGCTAGGCTGCCGCCGGCAGATGTGCCGCAGATCGGGGGCGACTC
GAGCAGAGAGGCCCTGGGGC

Downstream 100 bases:

>100_bases
CGCGCCGGTGGATGGGGGGAGCGCGACCGGCCGGCGCAAACCGCAGGAGATGTCGGCGCCGTAGCCGTACCCTGGTGACT
CGTGAGCAACGACCTGGACA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKIGVLTGGGDCPGLNAVIRAVVRKGVGVYGHSFVGFRDGWRGPLENDTVPLDVTAVRGILPRGGTILGSSRTNPFASAD
GPSRIRETLDRTGVDAIVAVGGEDTLGAAARLHAEGIPVVGVPKTIDNDLSATDYTFGFDTAVNIAMEAIDRLHTTAESH
HRVLVVEVMGRHAGWIALHSGMAAGANVILIPERPFDIDKVCAFVEQRFATRYAPIIVVAEGARPIAGTAMAREGELDAF
GHVRLQGISGLLETEIRGRTGRETRATVLGHVQRGGTPTAFDRWLATRFGLHAVDAVHEGDFGTMVALHGTRIDRVPLSE
ATGELKTVPEHLYGEAEVFFG

Sequences:

>Translated_341_residues
MKIGVLTGGGDCPGLNAVIRAVVRKGVGVYGHSFVGFRDGWRGPLENDTVPLDVTAVRGILPRGGTILGSSRTNPFASAD
GPSRIRETLDRTGVDAIVAVGGEDTLGAAARLHAEGIPVVGVPKTIDNDLSATDYTFGFDTAVNIAMEAIDRLHTTAESH
HRVLVVEVMGRHAGWIALHSGMAAGANVILIPERPFDIDKVCAFVEQRFATRYAPIIVVAEGARPIAGTAMAREGELDAF
GHVRLQGISGLLETEIRGRTGRETRATVLGHVQRGGTPTAFDRWLATRFGLHAVDAVHEGDFGTMVALHGTRIDRVPLSE
ATGELKTVPEHLYGEAEVFFG
>Mature_341_residues
MKIGVLTGGGDCPGLNAVIRAVVRKGVGVYGHSFVGFRDGWRGPLENDTVPLDVTAVRGILPRGGTILGSSRTNPFASAD
GPSRIRETLDRTGVDAIVAVGGEDTLGAAARLHAEGIPVVGVPKTIDNDLSATDYTFGFDTAVNIAMEAIDRLHTTAESH
HRVLVVEVMGRHAGWIALHSGMAAGANVILIPERPFDIDKVCAFVEQRFATRYAPIIVVAEGARPIAGTAMAREGELDAF
GHVRLQGISGLLETEIRGRTGRETRATVLGHVQRGGTPTAFDRWLATRFGLHAVDAVHEGDFGTMVALHGTRIDRVPLSE
ATGELKTVPEHLYGEAEVFFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=362, Percent_Identity=35.0828729281768, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI48762920, Length=363, Percent_Identity=35.2617079889807, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI266453768, Length=363, Percent_Identity=35.5371900826446, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI266453748, Length=363, Percent_Identity=35.5371900826446, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI4505749, Length=363, Percent_Identity=35.5371900826446, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI266453619, Length=363, Percent_Identity=35.5371900826446, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1790350, Length=326, Percent_Identity=38.3435582822086, Blast_Score=211, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI25147584, Length=362, Percent_Identity=30.6629834254144, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558788, Length=370, Percent_Identity=30, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI72003601, Length=204, Percent_Identity=34.8039215686275, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321679, Length=362, Percent_Identity=36.1878453038674, Blast_Score=179, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6323861, Length=367, Percent_Identity=34.8773841961853, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24652337, Length=362, Percent_Identity=33.4254143646409, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI17647809, Length=362, Percent_Identity=33.4254143646409, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI28573326, Length=362, Percent_Identity=32.8729281767956, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 36207; Mature: 36207

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVLTGGGDCPGLNAVIRAVVRKGVGVYGHSFVGFRDGWRGPLENDTVPLDVTAVRGI
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCEEEEHHHHHHC
LPRGGTILGSSRTNPFASADGPSRIRETLDRTGVDAIVAVGGEDTLGAAARLHAEGIPVV
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEE
GVPKTIDNDLSATDYTFGFDTAVNIAMEAIDRLHTTAESHHRVLVVEVMGRHAGWIALHS
ECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEC
GMAAGANVILIPERPFDIDKVCAFVEQRFATRYAPIIVVAEGARPIAGTAMAREGELDAF
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCC
GHVRLQGISGLLETEIRGRTGRETRATVLGHVQRGGTPTAFDRWLATRFGLHAVDAVHEG
CCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHCCC
DFGTMVALHGTRIDRVPLSEATGELKTVPEHLYGEAEVFFG
CCCEEEEEECCEECCCCCHHHCCCHHHCHHHHCCCEEEECC
>Mature Secondary Structure
MKIGVLTGGGDCPGLNAVIRAVVRKGVGVYGHSFVGFRDGWRGPLENDTVPLDVTAVRGI
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCEEEEHHHHHHC
LPRGGTILGSSRTNPFASADGPSRIRETLDRTGVDAIVAVGGEDTLGAAARLHAEGIPVV
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEE
GVPKTIDNDLSATDYTFGFDTAVNIAMEAIDRLHTTAESHHRVLVVEVMGRHAGWIALHS
ECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEC
GMAAGANVILIPERPFDIDKVCAFVEQRFATRYAPIIVVAEGARPIAGTAMAREGELDAF
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCC
GHVRLQGISGLLETEIRGRTGRETRATVLGHVQRGGTPTAFDRWLATRFGLHAVDAVHEG
CCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHCCC
DFGTMVALHGTRIDRVPLSEATGELKTVPEHLYGEAEVFFG
CCCEEEEEECCEECCCCCHHHCCCHHHCHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]