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The map label for this gene is nasB [H]

Identifier: 158313330

GI number: 158313330

Start: 1792049

End: 1794589

Strand: Direct

Name: nasB [H]

Synonym: Franean1_1492

Alternate gene names: 158313330

Gene position: 1792049-1794589 (Clockwise)

Preceding gene: 158313328

Following gene: 158313331

Centisome position: 19.95

GC content: 70.21

Gene sequence:

>2541_bases
ATGCCCGGAACCCCCCGGCAGCACATCGTCGTCGTCGGCAACGGCATGGTCGGCCAGCGGTTCGTGGAGACACTGCGGCA
ACGTGACACCGGGGGCCGGTTTTCGATCACCGTGGTGGGCGAGGAGCCGCGCCGTGCGTACGATCGCGTCGCGCTCTCGT
CCTACTTCGACGGCGTGAGTGCCGACGAACTGGACCTCGTGGCCGCGGGCATCTACGACAGCCCGCATGCGACGTTGCAT
CTGGACGAGTCCGTCACGGCGATCGACCGGGCCGCCTCGACCGTCACCACCAGCCGCGGTCGCACCCTCGGCTACGACGT
CCTCGTGCTGGCCACCGGCTCGTACCCGTTCGTTCCTCCTGTTCCGGGCAGGGACCTCGAAGGCTGCTTCGTCTACCGGA
CCCTCGACGATCTGGACGCGATCCGCGCCGCTGCCACTGCGGCGGCCGAACACACGCGTGGCCGCCGGGCCGGGCTGGTT
GTCGGCGGTGGGCTGCTCGGGCTCGAGGCGGCTCGGGCGCTGCGCCTGCTGGGCATGAGTCCGCACGTGGTGGAGCTGGC
GCCGCGGCTGATGCCGCTCCAGGTGGACGAGCCCGGCGGCGCGGTGCTCCGCCGACTGATCGAGCGCCTCGGCGTCACCG
TCCACACGGCGACGTCCGTGGCCGCCGTCGAGCCCAGCGGCGGACGGCTGCTCGCCACCCTGGCCAACGGCACCGAGCTC
GACCTGGACGTCGTCGTCTTCTCCGCCGGCATCCGTCCCCGCGACCAGTTGGCGCGTGAGTGTGGCCTTGCTGTCGGGCC
GCGCGGCGGCGTCGCGGTGGACGCACGCTGCCGCACGACCGACGAGTCCATCTATGCCATCGGGGAGTGCGCCGTCGTCG
ACGGTCATGTGTACGGCCTGGTCGCCCCGGGCTACGACATGGCCTCCGTGGTCGCCGAGCAGCTGCTCGGCCGGGACGCC
ACGTTCACCGGCGCGGACCTGAGCACGAAGCTCAAGCTGCTCGGTGTCGACGTCGCCAGCTTCGGTGACGCCCTCGCGGC
CACGGCGGGTGCGCTGGAGGTCCGTGTCGAGAACGCGGTGGAGAACACCTACGCCAAGATCGTCGTCTCCGACGACGCCA
GCACGCTGCTCGGCGGCATCCTCGTGGGTGACGCGAGCCGCTATGCGGCACTGCGCCCGCTGGTCGGGCGGCGGCTCCCG
GCGGACCCCGTCGCGATGATCGCCCCGACCGGCGGAGTCGACCTCGACGCGGGGGCCCTGCCTCGCGAGGCGCAGATCTG
CTCCTGCAACGACGTCTCCAAGGGCGCGATCCTGGACTCGATCGTCGAGCACGGTCTCACCGACGTCGCGGGGATCAAGG
CGTGTACGCGGGCCGGCACGACCTGCGGGTCCTGCGTGGCCCTGCTCAAGCCACTGCTGGCCGAGGCCGGCGTCGAGGTC
GGCACGGGGCTGTGTGAGCACTTCGACGTCACCCGGGCCGAGCTCTTCGAGATCGCCCGCAGTCAACGGATCGGCACCTT
CACCGAGCTCGTTCAGCGGCACGGACGTGGCCGCGGCTGCGACACGTGCAAGCCCGCCGTGGCCTCGATCCTGGCCAGCC
TGGGGCACGGCTATGTCCTGGACGGAGAACAGGCCGCACTGCAGGACACCAACGACCATTTCCTCGCGAACCTCCAGAAG
AACGGCACCTACTCGGTGGTGCCCCGCATCCCGGGCGGGGAGATCACGCCGGAGAAGCTGATCGTCATCGGCGAGGTGGC
CCGCGACTTCGGCCTCTACGTGAAGATCACCGGCGGGCAGCGCGTCGACCTGTTCGGGGCGCGGGTCGAGCAGCTGCCGC
TGATCTGGAGGCGTCTGGTCGACGCCGGGATGGAGTCCGGCCACGCCTACGGGAAGGCGATGCGGACCGTGAAGAGCTGC
GTCGGCTCGACGTGGTGCCGCTACGGTGTCCAGGACTCGGTCGGCCTCGCGATAGACCTCGAACTGCGCTACCGCGGCAT
CCGTGGCCCCCACAAGATCAAGGCTGGTGTCAGCGGCTGCGCCCGCGAATGTGCCGAGGCGCAGAGCAAGGACATAGGCG
TGATCGCCACCGATACCGGCTGGAACCTCTACGTCGGCGGCAACGGGGGAGCACGCCCGCGACACGCCGATCTGTTCCTC
ACCTCGGTCGACACCGAGACTCTCGTCAGGACCATCGACCGATTCATCATGTTCTACGCACGCACGGCCGACCGCCTGCA
GCGGACGTCCACCTGGGTCGAGTCGCTGTCCGCGGACGGTCAGGACGGACTGGCGTACCTGCGCGGCGTGCTCGTCGACG
ACAGCCTCGGCATCTGCGCCGAACTGGACGCCGCCATGGAATCGCACGTCGCGCACTACTCTGACGAGTGGGCCGACACC
CTCGCCGACCCCGAGCGGCTCAGGCGGTTCGTCAGTTTCGTGAACGCACCGGAGGTGCCGGACCCGTCCATCCAGTGGGC
GCCCGAGCGCGGCCAGATCAAACCGCTGATCGCCGGCCCGACCCTGGAGGTGGTGTCGTGA

Upstream 100 bases:

>100_bases
TCGGCGAAGTAACCGGGCCGACCGTCAGGGATAACTCGCGGTGGTCAGTGTGGCACTGACCAATGTGGCACCGACCCCAG
ATCGGAGAGGTGACTGCGTC

Downstream 100 bases:

>100_bases
GGACCGTGGAAGCCGCGAAGGCCGCGAAGGCCGCGGAGGCCGTGGAGGCCGTGGAGGCCGACGGGTGGGACAGCGTCTGC
GGCTCCCGCGACCTCCCCGT

Product: nitrite reductase (NAD(P)H), large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 846; Mature: 845

Protein sequence:

>846_residues
MPGTPRQHIVVVGNGMVGQRFVETLRQRDTGGRFSITVVGEEPRRAYDRVALSSYFDGVSADELDLVAAGIYDSPHATLH
LDESVTAIDRAASTVTTSRGRTLGYDVLVLATGSYPFVPPVPGRDLEGCFVYRTLDDLDAIRAAATAAAEHTRGRRAGLV
VGGGLLGLEAARALRLLGMSPHVVELAPRLMPLQVDEPGGAVLRRLIERLGVTVHTATSVAAVEPSGGRLLATLANGTEL
DLDVVVFSAGIRPRDQLARECGLAVGPRGGVAVDARCRTTDESIYAIGECAVVDGHVYGLVAPGYDMASVVAEQLLGRDA
TFTGADLSTKLKLLGVDVASFGDALAATAGALEVRVENAVENTYAKIVVSDDASTLLGGILVGDASRYAALRPLVGRRLP
ADPVAMIAPTGGVDLDAGALPREAQICSCNDVSKGAILDSIVEHGLTDVAGIKACTRAGTTCGSCVALLKPLLAEAGVEV
GTGLCEHFDVTRAELFEIARSQRIGTFTELVQRHGRGRGCDTCKPAVASILASLGHGYVLDGEQAALQDTNDHFLANLQK
NGTYSVVPRIPGGEITPEKLIVIGEVARDFGLYVKITGGQRVDLFGARVEQLPLIWRRLVDAGMESGHAYGKAMRTVKSC
VGSTWCRYGVQDSVGLAIDLELRYRGIRGPHKIKAGVSGCARECAEAQSKDIGVIATDTGWNLYVGGNGGARPRHADLFL
TSVDTETLVRTIDRFIMFYARTADRLQRTSTWVESLSADGQDGLAYLRGVLVDDSLGICAELDAAMESHVAHYSDEWADT
LADPERLRRFVSFVNAPEVPDPSIQWAPERGQIKPLIAGPTLEVVS

Sequences:

>Translated_846_residues
MPGTPRQHIVVVGNGMVGQRFVETLRQRDTGGRFSITVVGEEPRRAYDRVALSSYFDGVSADELDLVAAGIYDSPHATLH
LDESVTAIDRAASTVTTSRGRTLGYDVLVLATGSYPFVPPVPGRDLEGCFVYRTLDDLDAIRAAATAAAEHTRGRRAGLV
VGGGLLGLEAARALRLLGMSPHVVELAPRLMPLQVDEPGGAVLRRLIERLGVTVHTATSVAAVEPSGGRLLATLANGTEL
DLDVVVFSAGIRPRDQLARECGLAVGPRGGVAVDARCRTTDESIYAIGECAVVDGHVYGLVAPGYDMASVVAEQLLGRDA
TFTGADLSTKLKLLGVDVASFGDALAATAGALEVRVENAVENTYAKIVVSDDASTLLGGILVGDASRYAALRPLVGRRLP
ADPVAMIAPTGGVDLDAGALPREAQICSCNDVSKGAILDSIVEHGLTDVAGIKACTRAGTTCGSCVALLKPLLAEAGVEV
GTGLCEHFDVTRAELFEIARSQRIGTFTELVQRHGRGRGCDTCKPAVASILASLGHGYVLDGEQAALQDTNDHFLANLQK
NGTYSVVPRIPGGEITPEKLIVIGEVARDFGLYVKITGGQRVDLFGARVEQLPLIWRRLVDAGMESGHAYGKAMRTVKSC
VGSTWCRYGVQDSVGLAIDLELRYRGIRGPHKIKAGVSGCARECAEAQSKDIGVIATDTGWNLYVGGNGGARPRHADLFL
TSVDTETLVRTIDRFIMFYARTADRLQRTSTWVESLSADGQDGLAYLRGVLVDDSLGICAELDAAMESHVAHYSDEWADT
LADPERLRRFVSFVNAPEVPDPSIQWAPERGQIKPLIAGPTLEVVS
>Mature_845_residues
PGTPRQHIVVVGNGMVGQRFVETLRQRDTGGRFSITVVGEEPRRAYDRVALSSYFDGVSADELDLVAAGIYDSPHATLHL
DESVTAIDRAASTVTTSRGRTLGYDVLVLATGSYPFVPPVPGRDLEGCFVYRTLDDLDAIRAAATAAAEHTRGRRAGLVV
GGGLLGLEAARALRLLGMSPHVVELAPRLMPLQVDEPGGAVLRRLIERLGVTVHTATSVAAVEPSGGRLLATLANGTELD
LDVVVFSAGIRPRDQLARECGLAVGPRGGVAVDARCRTTDESIYAIGECAVVDGHVYGLVAPGYDMASVVAEQLLGRDAT
FTGADLSTKLKLLGVDVASFGDALAATAGALEVRVENAVENTYAKIVVSDDASTLLGGILVGDASRYAALRPLVGRRLPA
DPVAMIAPTGGVDLDAGALPREAQICSCNDVSKGAILDSIVEHGLTDVAGIKACTRAGTTCGSCVALLKPLLAEAGVEVG
TGLCEHFDVTRAELFEIARSQRIGTFTELVQRHGRGRGCDTCKPAVASILASLGHGYVLDGEQAALQDTNDHFLANLQKN
GTYSVVPRIPGGEITPEKLIVIGEVARDFGLYVKITGGQRVDLFGARVEQLPLIWRRLVDAGMESGHAYGKAMRTVKSCV
GSTWCRYGVQDSVGLAIDLELRYRGIRGPHKIKAGVSGCARECAEAQSKDIGVIATDTGWNLYVGGNGGARPRHADLFLT
SVDTETLVRTIDRFIMFYARTADRLQRTSTWVESLSADGQDGLAYLRGVLVDDSLGICAELDAAMESHVAHYSDEWADTL
ADPERLRRFVSFVNAPEVPDPSIQWAPERGQIKPLIAGPTLEVVS

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=324, Percent_Identity=25.6172839506173, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI226437568, Length=324, Percent_Identity=25.6172839506173, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI21389617, Length=324, Percent_Identity=25.6172839506173, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1789765, Length=839, Percent_Identity=51.0131108462455, Blast_Score=828, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=331, Percent_Identity=31.7220543806647, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI1788892, Length=287, Percent_Identity=27.1777003484321, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=283, Percent_Identity=28.2685512367491, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281359715, Length=320, Percent_Identity=26.5625, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281359713, Length=320, Percent_Identity=26.5625, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24639250, Length=320, Percent_Identity=26.5625, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI18543267, Length=320, Percent_Identity=26.5625, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24639252, Length=320, Percent_Identity=26.5625, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24639257, Length=320, Percent_Identity=26.5625, Blast_Score=80, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585130, Length=331, Percent_Identity=22.3564954682779, Blast_Score=76, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 89662; Mature: 89531

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00365 NIR_SIR ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTPRQHIVVVGNGMVGQRFVETLRQRDTGGRFSITVVGEEPRRAYDRVALSSYFDGVS
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCC
ADELDLVAAGIYDSPHATLHLDESVTAIDRAASTVTTSRGRTLGYDVLVLATGSYPFVPP
CCCHHEEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEECEEEEEEEECCCCCCCC
VPGRDLEGCFVYRTLDDLDAIRAAATAAAEHTRGRRAGLVVGGGLLGLEAARALRLLGMS
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCC
PHVVELAPRLMPLQVDEPGGAVLRRLIERLGVTVHTATSVAAVEPSGGRLLATLANGTEL
CHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCEEEECCEEEEECCCCCEEEEEECCCCCC
DLDVVVFSAGIRPRDQLARECGLAVGPRGGVAVDARCRTTDESIYAIGECAVVDGHVYGL
EEEEEEEECCCCCHHHHHHHCCCEECCCCCEEEECEECCCCCCEEEEEEEEEECCEEEEE
VAPGYDMASVVAEQLLGRDATFTGADLSTKLKLLGVDVASFGDALAATAGALEVRVENAV
EECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHCHHHHHCCCCEEEEEHHHH
ENTYAKIVVSDDASTLLGGILVGDASRYAALRPLVGRRLPADPVAMIAPTGGVDLDAGAL
CCCEEEEEECCCHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
PREAQICSCNDVSKGAILDSIVEHGLTDVAGIKACTRAGTTCGSCVALLKPLLAEAGVEV
CCCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
GTGLCEHFDVTRAELFEIARSQRIGTFTELVQRHGRGRGCDTCKPAVASILASLGHGYVL
HCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEE
DGEQAALQDTNDHFLANLQKNGTYSVVPRIPGGEITPEKLIVIGEVARDFGLYVKITGGQ
CCCHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHEEEEEEHHHCCCEEEEECCCC
RVDLFGARVEQLPLIWRRLVDAGMESGHAYGKAMRTVKSCVGSTWCRYGVQDSVGLAIDL
EEEHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ELRYRGIRGPHKIKAGVSGCARECAEAQSKDIGVIATDTGWNLYVGGNGGARPRHADLFL
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEE
TSVDTETLVRTIDRFIMFYARTADRLQRTSTWVESLSADGQDGLAYLRGVLVDDSLGICA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHH
ELDAAMESHVAHYSDEWADTLADPERLRRFVSFVNAPEVPDPSIQWAPERGQIKPLIAGP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEEECC
TLEVVS
CEEECC
>Mature Secondary Structure 
PGTPRQHIVVVGNGMVGQRFVETLRQRDTGGRFSITVVGEEPRRAYDRVALSSYFDGVS
CCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCC
ADELDLVAAGIYDSPHATLHLDESVTAIDRAASTVTTSRGRTLGYDVLVLATGSYPFVPP
CCCHHEEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEECEEEEEEEECCCCCCCC
VPGRDLEGCFVYRTLDDLDAIRAAATAAAEHTRGRRAGLVVGGGLLGLEAARALRLLGMS
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCC
PHVVELAPRLMPLQVDEPGGAVLRRLIERLGVTVHTATSVAAVEPSGGRLLATLANGTEL
CHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCEEEECCEEEEECCCCCEEEEEECCCCCC
DLDVVVFSAGIRPRDQLARECGLAVGPRGGVAVDARCRTTDESIYAIGECAVVDGHVYGL
EEEEEEEECCCCCHHHHHHHCCCEECCCCCEEEECEECCCCCCEEEEEEEEEECCEEEEE
VAPGYDMASVVAEQLLGRDATFTGADLSTKLKLLGVDVASFGDALAATAGALEVRVENAV
EECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHCHHHHHCCCCEEEEEHHHH
ENTYAKIVVSDDASTLLGGILVGDASRYAALRPLVGRRLPADPVAMIAPTGGVDLDAGAL
CCCEEEEEECCCHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
PREAQICSCNDVSKGAILDSIVEHGLTDVAGIKACTRAGTTCGSCVALLKPLLAEAGVEV
CCCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
GTGLCEHFDVTRAELFEIARSQRIGTFTELVQRHGRGRGCDTCKPAVASILASLGHGYVL
HCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEE
DGEQAALQDTNDHFLANLQKNGTYSVVPRIPGGEITPEKLIVIGEVARDFGLYVKITGGQ
CCCHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHEEEEEEHHHCCCEEEEECCCC
RVDLFGARVEQLPLIWRRLVDAGMESGHAYGKAMRTVKSCVGSTWCRYGVQDSVGLAIDL
EEEHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ELRYRGIRGPHKIKAGVSGCARECAEAQSKDIGVIATDTGWNLYVGGNGGARPRHADLFL
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEE
TSVDTETLVRTIDRFIMFYARTADRLQRTSTWVESLSADGQDGLAYLRGVLVDDSLGICA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHH
ELDAAMESHVAHYSDEWADTLADPERLRRFVSFVNAPEVPDPSIQWAPERGQIKPLIAGP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEEEECC
TLEVVS
CEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]