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The map label for this gene is truB [H]

Identifier: 158313038

GI number: 158313038

Start: 1410712

End: 1411653

Strand: Direct

Name: truB [H]

Synonym: Franean1_1186

Alternate gene names: 158313038

Gene position: 1410712-1411653 (Clockwise)

Preceding gene: 158313037

Following gene: 158313039

Centisome position: 15.71

GC content: 77.28

Gene sequence:

>942_bases
GTGCCTTCTTCTGAACGTCCCACGGCGGCCGGACGTCCCACGGCGGCCGGACGTCCCACGGCGGCCGAGCGTCCCGCGGC
CGTCGACGGCCTCGTCGTCGTGGACAAGCCCGCCGGCTGGACGTCGCACGACGTCGTCGCGCGGTCCCGGCGCCTGCTGG
GCACCCGCCGCATCGGCCACGCCGGCACCCTGGACCCGATGGCGACCGGTGTGCTCGTGCTCGGCGTCGGCCGAGGAACC
CGCCTGCTCGGCCGACTGGCGCTGACCGCCAAGGTGTACGAGGCCACGATCCGGCTCGGCCGCACCACCACGACGGACGA
CGCCGAGGGCGACCCGGTGGCGGACCGCCCGGTCGATGTCGGCGCCGGCCCGCTCGCAGCGGCGATGGCGGAGCTGACCG
GGGAGATCGACCAGGTCCCGTCCAGTGTCAGCGCGGTGAAGGTCGCCGGGGTGCGGGCCTACGACCGGGTCCGGGCCGGC
GAGGAGGTGACGCTCGCGCCGCGGCGGGTCACCGTCGGCCGGTTCGAGCTGCTCGCCCGGCGCGGCGAGACCGACCTGGA
CGTCGTCGTCGCCTGCTCGAGCGGGACGTACATCCGCGCGCTCGCCCGGGATCTGGGGGCCGCGCTGGGCTGCGGCGCCC
ACCTCACCGCGCTGCGGCGCACCGAGGTCGGGCCGTTCGGGCTGGCCGAGGCGGTGACCCTCGACGCGCTCGCCGAGGCC
GCGAACGCGGCCGTCGTGCCGCTGGGGGCAGCGGTGGCGGCGACCTTCCCGCGCCGCGACGTGGACGGGGAGCAGGCCCG
GGACGTCGCCTACGGGCGGCGGCTGCCCATGCTCGGCAGCCCGGGGCCGTACGGCGTGTTCGGGCCGGACGGAGCGCTCC
TCGCGCTGTTCGAGGAACGGGACGGCGTGGCCCGCCCGATCGTCGTCTTCGCGCCCGCGTAG

Upstream 100 bases:

>100_bases
TCGCGGCCGGCATCTCGTCGTCGGCGCCGCTGGAGACCGTGATGCGTGTCTTCCGCGAGGAGCTCACCCGCGCTCCCCGC
CTCGCCGGGTGATCCCTCCC

Downstream 100 bases:

>100_bases
CACCCCGCCTGCGCTGTGTCACCCGCACTCACCTGGTGTTCCGTCGGTGTGCGGACCCGGTGCGTCCACCCGGCGTGCGC
ATCAGGCGCGGGTCCGTCGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MPSSERPTAAGRPTAAGRPTAAERPAAVDGLVVVDKPAGWTSHDVVARSRRLLGTRRIGHAGTLDPMATGVLVLGVGRGT
RLLGRLALTAKVYEATIRLGRTTTTDDAEGDPVADRPVDVGAGPLAAAMAELTGEIDQVPSSVSAVKVAGVRAYDRVRAG
EEVTLAPRRVTVGRFELLARRGETDLDVVVACSSGTYIRALARDLGAALGCGAHLTALRRTEVGPFGLAEAVTLDALAEA
ANAAVVPLGAAVAATFPRRDVDGEQARDVAYGRRLPMLGSPGPYGVFGPDGALLALFEERDGVARPIVVFAPA

Sequences:

>Translated_313_residues
MPSSERPTAAGRPTAAGRPTAAERPAAVDGLVVVDKPAGWTSHDVVARSRRLLGTRRIGHAGTLDPMATGVLVLGVGRGT
RLLGRLALTAKVYEATIRLGRTTTTDDAEGDPVADRPVDVGAGPLAAAMAELTGEIDQVPSSVSAVKVAGVRAYDRVRAG
EEVTLAPRRVTVGRFELLARRGETDLDVVVACSSGTYIRALARDLGAALGCGAHLTALRRTEVGPFGLAEAVTLDALAEA
ANAAVVPLGAAVAATFPRRDVDGEQARDVAYGRRLPMLGSPGPYGVFGPDGALLALFEERDGVARPIVVFAPA
>Mature_312_residues
PSSERPTAAGRPTAAGRPTAAERPAAVDGLVVVDKPAGWTSHDVVARSRRLLGTRRIGHAGTLDPMATGVLVLGVGRGTR
LLGRLALTAKVYEATIRLGRTTTTDDAEGDPVADRPVDVGAGPLAAAMAELTGEIDQVPSSVSAVKVAGVRAYDRVRAGE
EVTLAPRRVTVGRFELLARRGETDLDVVVACSSGTYIRALARDLGAALGCGAHLTALRRTEVGPFGLAEAVTLDALAEAA
NAAVVPLGAAVAATFPRRDVDGEQARDVAYGRRLPMLGSPGPYGVFGPDGALLALFEERDGVARPIVVFAPA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=271, Percent_Identity=31.7343173431734, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI4503337, Length=196, Percent_Identity=32.6530612244898, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI215599015, Length=196, Percent_Identity=32.6530612244898, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI2367200, Length=218, Percent_Identity=41.2844036697248, Blast_Score=168, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17553978, Length=196, Percent_Identity=30.6122448979592, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=218, Percent_Identity=32.1100917431193, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=144, Percent_Identity=32.6388888888889, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=30.6122448979592, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=30.6122448979592, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=30.6122448979592, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=30.6122448979592, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=30.6122448979592, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=30.6122448979592, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780 [H]

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 32249; Mature: 32118

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSERPTAAGRPTAAGRPTAAERPAAVDGLVVVDKPAGWTSHDVVARSRRLLGTRRIGH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
AGTLDPMATGVLVLGVGRGTRLLGRLALTAKVYEATIRLGRTTTTDDAEGDPVADRPVDV
CCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GAGPLAAAMAELTGEIDQVPSSVSAVKVAGVRAYDRVRAGEEVTLAPRRVTVGRFELLAR
CCCHHHHHHHHHHHHHHHCCCHHHHEEECCHHHHHHHCCCCEEEECCCEEEHHHHHHHHH
RGETDLDVVVACSSGTYIRALARDLGAALGCGAHLTALRRTEVGPFGLAEAVTLDALAEA
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ANAAVVPLGAAVAATFPRRDVDGEQARDVAYGRRLPMLGSPGPYGVFGPDGALLALFEER
HCCEEEECCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECC
DGVARPIVVFAPA
CCCCCCEEEEECC
>Mature Secondary Structure 
PSSERPTAAGRPTAAGRPTAAERPAAVDGLVVVDKPAGWTSHDVVARSRRLLGTRRIGH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
AGTLDPMATGVLVLGVGRGTRLLGRLALTAKVYEATIRLGRTTTTDDAEGDPVADRPVDV
CCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GAGPLAAAMAELTGEIDQVPSSVSAVKVAGVRAYDRVRAGEEVTLAPRRVTVGRFELLAR
CCCHHHHHHHHHHHHHHHCCCHHHHEEECCHHHHHHHCCCCEEEECCCEEEHHHHHHHHH
RGETDLDVVVACSSGTYIRALARDLGAALGCGAHLTALRRTEVGPFGLAEAVTLDALAEA
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ANAAVVPLGAAVAATFPRRDVDGEQARDVAYGRRLPMLGSPGPYGVFGPDGALLALFEER
HCCEEEECCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECC
DGVARPIVVFAPA
CCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA