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The map label for this gene is rlmN

Identifier: 158313018

GI number: 158313018

Start: 1388549

End: 1389706

Strand: Direct

Name: rlmN

Synonym: Franean1_1166

Alternate gene names: 158313018

Gene position: 1388549-1389706 (Clockwise)

Preceding gene: 158313017

Following gene: 158313019

Centisome position: 15.46

GC content: 74.18

Gene sequence:

>1158_bases
ATGACAGCCACGACCGCCGAATCCGGAAACCTCCTGCCACTGGTGTCAGGGCGGTCCAGGCCGCCGCGCCACCTCGCGGA
CCTCTCCCGGGACGAGCGGCGGGCCGTGGCCACGTCACTGGGCCTGCCGGCGTTCCGTGCCGACCAGCTGGCCCGCCACT
ACTTCACCCACCACCTGCGTGCCGACGACGCAGACCTGATGACGGACCTGCCCGCCTCGATCCGCCCCGCGCTCGTCGAG
GCGATGCTGCCGAGGCTGCTCACGCCGGCCACCGCCCTCGACTGCGACGGTGGCCAGACCCGCAAGACCGTCTGGCGTAC
CGTCGACGGCGCCAAGATCGAGTCGGTGCTGATGCGCTACCCGCAGCGGGCGACGGTCTGTGTCTCGAGCCAGGCGGGCT
GCGGCATGGGATGCCCGTTCTGCGCGACGGGCCAGGGCGGGCTGACCCGCAACCTCTCGACCGCCGAGATTGTCGAACAG
GTGGTGGACGCGGCTCGAACGATGGCGGCCCGAACGACGGCCGAGGGTGGCCTGCCCGGTGGTCCCGGCAGGCTGTCGAA
CGTGGTGTTCATGGGCATGGGGGAGCCGCTGGCGAACTACGCCGCCCTCCTCGCCGCCCTGCACCGCCTCATCGACCCGG
CGCCCGACGGGCTGGGCCTCTCCGCCCGCGGGCTCACGGTCAGCACGGTGGGCCTGGTCCCGGCGATCCGTCGCCTCGCC
GGTGAGGGGCTGCCGGTGACCCTCGCCGTGTCCCTGCACGCCCCGGATGACGAGCTGCGCGACGAGCTCGTCCCGATCAA
CACCCGATGGCCGGTGGCCGAGGTGCTCGCCGCCGCGTGGGAGTACGCCCGGGTGACAGGGCGGCGCGTGAGCATCGAGT
ACGCCCTGATCGACGGCGTCAACGACTCGCCCGAGCGCGCGGACGCGCTGGCCGCCCTGCTGGTCGGCCAGCTCGCGCAC
GTGAACCTCATCCCGCTCAACCCGACGGGCGGCTCGTCCTGGCAGGCGAGCGCCCCGCGCGGCCAGCGTGTCTTCGTGGA
GCGCCTGCGGGCCCGCGGGGTGGCGGCCACCGTCCGGGACACCCGGGGCCGGGAGATCGCCGCGGCCTGCGGGCAACTCG
CCGCGGAGCCGCCCGTGCGCTCGAGGGCGGGTCGGTAG

Upstream 100 bases:

>100_bases
GTGTACCGCGCCCGTTGCCTGGCTGCTCATCACCGCGTTCCTCGGCTGACCGATCACCGATTCGTCGCGTGCCCGGGGCT
CCTGGCAGACTGGACTGGCC

Downstream 100 bases:

>100_bases
TCGTGGCCACCGGGAACTCCGCCGGTCGTCGGCGTAGCCACGGCCGGGCCGGGGCCGGCTCCGCGCGGTTGGGGGCCGCC
CGGCAGGGGCTGGCCCGGTC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTATTAESGNLLPLVSGRSRPPRHLADLSRDERRAVATSLGLPAFRADQLARHYFTHHLRADDADLMTDLPASIRPALVE
AMLPRLLTPATALDCDGGQTRKTVWRTVDGAKIESVLMRYPQRATVCVSSQAGCGMGCPFCATGQGGLTRNLSTAEIVEQ
VVDAARTMAARTTAEGGLPGGPGRLSNVVFMGMGEPLANYAALLAALHRLIDPAPDGLGLSARGLTVSTVGLVPAIRRLA
GEGLPVTLAVSLHAPDDELRDELVPINTRWPVAEVLAAAWEYARVTGRRVSIEYALIDGVNDSPERADALAALLVGQLAH
VNLIPLNPTGGSSWQASAPRGQRVFVERLRARGVAATVRDTRGREIAAACGQLAAEPPVRSRAGR

Sequences:

>Translated_385_residues
MTATTAESGNLLPLVSGRSRPPRHLADLSRDERRAVATSLGLPAFRADQLARHYFTHHLRADDADLMTDLPASIRPALVE
AMLPRLLTPATALDCDGGQTRKTVWRTVDGAKIESVLMRYPQRATVCVSSQAGCGMGCPFCATGQGGLTRNLSTAEIVEQ
VVDAARTMAARTTAEGGLPGGPGRLSNVVFMGMGEPLANYAALLAALHRLIDPAPDGLGLSARGLTVSTVGLVPAIRRLA
GEGLPVTLAVSLHAPDDELRDELVPINTRWPVAEVLAAAWEYARVTGRRVSIEYALIDGVNDSPERADALAALLVGQLAH
VNLIPLNPTGGSSWQASAPRGQRVFVERLRARGVAATVRDTRGREIAAACGQLAAEPPVRSRAGR
>Mature_384_residues
TATTAESGNLLPLVSGRSRPPRHLADLSRDERRAVATSLGLPAFRADQLARHYFTHHLRADDADLMTDLPASIRPALVEA
MLPRLLTPATALDCDGGQTRKTVWRTVDGAKIESVLMRYPQRATVCVSSQAGCGMGCPFCATGQGGLTRNLSTAEIVEQV
VDAARTMAARTTAEGGLPGGPGRLSNVVFMGMGEPLANYAALLAALHRLIDPAPDGLGLSARGLTVSTVGLVPAIRRLAG
EGLPVTLAVSLHAPDDELRDELVPINTRWPVAEVLAAAWEYARVTGRRVSIEYALIDGVNDSPERADALAALLVGQLAHV
NLIPLNPTGGSSWQASAPRGQRVFVERLRARGVAATVRDTRGREIAAACGQLAAEPPVRSRAGR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=363, Percent_Identity=39.3939393939394, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_FRASN (A8L6D8)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505526.1
- ProteinModelPortal:   A8L6D8
- GeneID:   5669579
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1166
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14459
- BioCyc:   FSP1855:FRANEAN1_1166-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40692; Mature: 40561

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATTAESGNLLPLVSGRSRPPRHLADLSRDERRAVATSLGLPAFRADQLARHYFTHHLR
CCCCCCCCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
ADDADLMTDLPASIRPALVEAMLPRLLTPATALDCDGGQTRKTVWRTVDGAKIESVLMRY
CCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCHHHHHHHHHC
PQRATVCVSSQAGCGMGCPFCATGQGGLTRNLSTAEIVEQVVDAARTMAARTTAEGGLPG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GPGRLSNVVFMGMGEPLANYAALLAALHRLIDPAPDGLGLSARGLTVSTVGLVPAIRRLA
CCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHC
GEGLPVTLAVSLHAPDDELRDELVPINTRWPVAEVLAAAWEYARVTGRRVSIEYALIDGV
CCCCCEEEEEEEECCCHHHHHCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECC
NDSPERADALAALLVGQLAHVNLIPLNPTGGSSWQASAPRGQRVFVERLRARGVAATVRD
CCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHH
TRGREIAAACGQLAAEPPVRSRAGR
CCCCHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
TATTAESGNLLPLVSGRSRPPRHLADLSRDERRAVATSLGLPAFRADQLARHYFTHHLR
CCCCCCCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
ADDADLMTDLPASIRPALVEAMLPRLLTPATALDCDGGQTRKTVWRTVDGAKIESVLMRY
CCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCHHHHHHHHHC
PQRATVCVSSQAGCGMGCPFCATGQGGLTRNLSTAEIVEQVVDAARTMAARTTAEGGLPG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GPGRLSNVVFMGMGEPLANYAALLAALHRLIDPAPDGLGLSARGLTVSTVGLVPAIRRLA
CCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHC
GEGLPVTLAVSLHAPDDELRDELVPINTRWPVAEVLAAAWEYARVTGRRVSIEYALIDGV
CCCCCEEEEEEEECCCHHHHHCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECC
NDSPERADALAALLVGQLAHVNLIPLNPTGGSSWQASAPRGQRVFVERLRARGVAATVRD
CCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHH
TRGREIAAACGQLAAEPPVRSRAGR
CCCCHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA