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The map label for this gene is trmD

Identifier: 158313003

GI number: 158313003

Start: 1373104

End: 1373955

Strand: Direct

Name: trmD

Synonym: Franean1_1151

Alternate gene names: 158313003

Gene position: 1373104-1373955 (Clockwise)

Preceding gene: 158313002

Following gene: 158313004

Centisome position: 15.29

GC content: 72.18

Gene sequence:

>852_bases
ATGCGCATCGACGTCGTGACGATCTTCCCGGACTATCTGCGCCCGTTGGAGCTGGCCCTGGTGGGCCGCGCCGCGTCCCG
GGACCTCGTCGACATCCAGACCCACGACCTGCGCGCGTGGACGCACGACGTCCACCGCACCGTCGATGATTCCCCGTACG
GCGGGGGGCCGGGCATGGTCATGCGCCCGGAGCCGTGGGACGCGGCGCTGCGCGACATCGTGGCCCGTGATCCGTCGCGT
CGTCCCCGAGTCGTGGTGCCCACCCCGGCCGGTGTTCCGTTCACCCAGAGCTACGCCGCCGAGCTGGCGGAACAGGACTG
GCTGATCTTCTGCTGCGGCCGCTACGAGGGCATCGACGGCCGGGTGGTGGACGCCTGGGCCGACGACGAGATCTCGATCG
GCGACTACGTGCTCGCCGGCGGCGAGGTCGCCACACTCGTCATGGTCGAGGCGGTGACGCGGCTGCTTCCCGGCGTCGTC
GGCAACGCGGAGTCGATCATCGACGACTCCTTCGCCCACGGGCTGCTCGAGGGGCCGGTCTACACCAGGCCCCCGGTGTG
GGAGGGCCGCGCGGTGCCCGAGATCCTCCGCTCCGGCGACCACGCGGCGATCGCCCGGTGGCGGCGGGAGGAGGCGCTGC
GCCGGACCTGCCGAAGGCGCCCGGAGCTGCTCGACCGGATCGAGCTGTCGGACGCCGACCGCGCGGTGGTGGCGAGCACC
ATCGTGGCCGCCCATGGCGGCGGGGACGACCCCCCTGTAACGGGTGTCGAGGACGCGGTGACGATTGCCGAGAGAGGCAC
GATCGGTGACGGGCCGGCTCCGTCGGGTTGTGCGTCGAGCCACTGTTCCTGA

Upstream 100 bases:

>100_bases
CGTCACCGCCCCACCTCGCCACCGCCCCCGCTCCGCCACCGCCCCGTTTCGAGGCGCCGCCCACCGGGTGCGGCACACCT
CCCTCTGACGAAGGCAGCGG

Downstream 100 bases:

>100_bases
GCTTCCTGAGTGCTCCGTCGCCTGACTTCTTCGCCGTCCTCTGCCATCGCCGAGCCGGGCGGCCGACGTCGAGCGGCCCG
GTCGAGCCGCCGACCAGCCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRIDVVTIFPDYLRPLELALVGRAASRDLVDIQTHDLRAWTHDVHRTVDDSPYGGGPGMVMRPEPWDAALRDIVARDPSR
RPRVVVPTPAGVPFTQSYAAELAEQDWLIFCCGRYEGIDGRVVDAWADDEISIGDYVLAGGEVATLVMVEAVTRLLPGVV
GNAESIIDDSFAHGLLEGPVYTRPPVWEGRAVPEILRSGDHAAIARWRREEALRRTCRRRPELLDRIELSDADRAVVAST
IVAAHGGGDDPPVTGVEDAVTIAERGTIGDGPAPSGCASSHCS

Sequences:

>Translated_283_residues
MRIDVVTIFPDYLRPLELALVGRAASRDLVDIQTHDLRAWTHDVHRTVDDSPYGGGPGMVMRPEPWDAALRDIVARDPSR
RPRVVVPTPAGVPFTQSYAAELAEQDWLIFCCGRYEGIDGRVVDAWADDEISIGDYVLAGGEVATLVMVEAVTRLLPGVV
GNAESIIDDSFAHGLLEGPVYTRPPVWEGRAVPEILRSGDHAAIARWRREEALRRTCRRRPELLDRIELSDADRAVVAST
IVAAHGGGDDPPVTGVEDAVTIAERGTIGDGPAPSGCASSHCS
>Mature_283_residues
MRIDVVTIFPDYLRPLELALVGRAASRDLVDIQTHDLRAWTHDVHRTVDDSPYGGGPGMVMRPEPWDAALRDIVARDPSR
RPRVVVPTPAGVPFTQSYAAELAEQDWLIFCCGRYEGIDGRVVDAWADDEISIGDYVLAGGEVATLVMVEAVTRLLPGVV
GNAESIIDDSFAHGLLEGPVYTRPPVWEGRAVPEILRSGDHAAIARWRREEALRRTCRRRPELLDRIELSDADRAVVAST
IVAAHGGGDDPPVTGVEDAVTIAERGTIGDGPAPSGCASSHCS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=242, Percent_Identity=45.0413223140496, Blast_Score=204, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_FRASN (A8L5A5)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505511.1
- ProteinModelPortal:   A8L5A5
- SMR:   A8L5A5
- GeneID:   5669564
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1151
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   FSP1855:FRANEAN1_1151-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 30643; Mature: 30643

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVVTIFPDYLRPLELALVGRAASRDLVDIQTHDLRAWTHDVHRTVDDSPYGGGPGMV
CEEEEEEECHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCE
MRPEPWDAALRDIVARDPSRRPRVVVPTPAGVPFTQSYAAELAEQDWLIFCCGRYEGIDG
ECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCC
RVVDAWADDEISIGDYVLAGGEVATLVMVEAVTRLLPGVVGNAESIIDDSFAHGLLEGPV
EEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
YTRPPVWEGRAVPEILRSGDHAAIARWRREEALRRTCRRRPELLDRIELSDADRAVVAST
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHH
IVAAHGGGDDPPVTGVEDAVTIAERGTIGDGPAPSGCASSHCS
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIDVVTIFPDYLRPLELALVGRAASRDLVDIQTHDLRAWTHDVHRTVDDSPYGGGPGMV
CEEEEEEECHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCE
MRPEPWDAALRDIVARDPSRRPRVVVPTPAGVPFTQSYAAELAEQDWLIFCCGRYEGIDG
ECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCC
RVVDAWADDEISIGDYVLAGGEVATLVMVEAVTRLLPGVVGNAESIIDDSFAHGLLEGPV
EEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
YTRPPVWEGRAVPEILRSGDHAAIARWRREEALRRTCRRRPELLDRIELSDADRAVVAST
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHH
IVAAHGGGDDPPVTGVEDAVTIAERGTIGDGPAPSGCASSHCS
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA