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The map label for this gene is gatB [H]

Identifier: 158312941

GI number: 158312941

Start: 1296335

End: 1297852

Strand: Direct

Name: gatB [H]

Synonym: Franean1_1087

Alternate gene names: 158312941

Gene position: 1296335-1297852 (Clockwise)

Preceding gene: 158312940

Following gene: 158312942

Centisome position: 14.43

GC content: 72.79

Gene sequence:

>1518_bases
GTGAGCACATCGGCTCCCGTCATCGCCACCCCGTCCTACGAGGACGCCACCGCCCGGTACGAGTCGGTGATCGGGCTGGA
GACCCACGTCGAGCTCGGCACCGCGTCCAAGATGTTCTGCGGGTGCGCGACCGCCTTCGGTGCGGAGCCGAACACCCAGG
TGTGCCCGGTCTGCCTGGGCCTGCCCGGTGCGCTGCCCGTGGTCAACGAGGCCGCGGTGCGTTTCGCGACGCTGATCGGG
CTGGCGCTGAACTGTTCGATCGCGTCCTGGTGCCGGTTCGCCCGGAAGAACTACTTCTACCCGGACATGCCGAAGAACTT
CCAGATCAGCCAGTACGACGAGCCACTGTGCTCGAACGGCTGGCTCGACGTCGAGGTCGAGGGCGAGATCCACCGGGTCG
GGATCACCCGGGTTCACCTCGAGGAGGACACCGGCAAGTCCCTGCACGTCGGCGGGGCGACCGGCCGCATCCACGGGGCG
ACCCACTCGCTGGTCGACTACAACCGCGCCGGCATCCCGCTGGTCGAGATCGTCACCGAGCCCGACATCCGCTCGCCGGA
GGTCGCCCGCGCCTACGTCGACGAGCTGCGTGAGCTGTGCCGCGCGCTGGCCGTCTCGGACGTCCGGATGGACCAGGGCT
CGCTGCGCTGCGACGCGAACGTCTCGCTGCGGCTGCGCCCCGCGCCCGGTGAGCCCGACGCGCCGTTCGGCACCCGGTCG
GAGACGAAGAACCTGAACTCGCTGCGCTCGGTCGAGCGGGCGGTGCGGTTCGAGGTCGTCCGGCAGGCCGCGCTGCTGGA
CGCGGGCGACCGGGTCGTGCAGGAGACCCGGCACTTCGACGAGGCCTCCGGGCGGACGTCGTCGGGCCGGTCGAAGGAGG
AGGCGACCGACTACCGGTACTTCCCCGAGCCGGACCTGACGCCCCTCGCGCTCACCCACGAGTACATCGAGGCCGTGCGC
GCGGTGCTGCCGGAGCTGCCGGCCCAGCGGCGCGTCCGGCTCATCGCCGAGCACGGGTACACGGTGCGCGACATCCAGGA
GATGCGCAACGCCGGGGTGCTCGACCTGGTCGAGGCGACGGTGGCCGCCGGGGCCAGCCCGCCCGCGGCCCGCAAGTGGT
GGCTCAACGAGCTCGCCCGGCGCGCGGCGGACGCCGGGGTCGAGCCGCGAGAGCTGGCGATCGGCCCGGCCGACGTGGCG
CGGATCTGCGAGCTGGTCGCGGCCGGGCAGCTCACCGACGCGCTCGGACGCAAGGTCATCGACGGGGTGCTCGCCGGTGA
CGGTTCAGCCGACGAGGTCATCGCCGCCCGTGACCTGGCTGTGGTCTCCGACGACTCGGCGCTGCGCGACGCGGTCGACA
CGGCGATCGCCGGCGCGCCCGACATCGCCGAGAAGGTGCGGGGCGGCAAGGTGAACGCCGTCGGCCCGCTGGTCGGCGCG
GTCATGAAGGCGATGCGCGGCCAGGCCGACGCCGCGGCCGTCCGCCGCCTGCTGCTGGAGCGCCTCGAGGCCTCCTGA

Upstream 100 bases:

>100_bases
CGCTGAGTTACGTCCACGTCCGTGACACCCGCTCCGCTCCGCGCCCGCCCGCCGCGGGTGAGGAAGACGGCGTCGACGGC
ATCGAAGAGGAGTCATCCCG

Downstream 100 bases:

>100_bases
CGCATCCGGGACGGCGACGTGGGTCACATCACTGGTCCCGTTCCAGCCCCGGGCCCGGGGCGGCCGGTGTGCGACGATCG
TCAGTGATGCAATCGACCAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSTSAPVIATPSYEDATARYESVIGLETHVELGTASKMFCGCATAFGAEPNTQVCPVCLGLPGALPVVNEAAVRFATLIG
LALNCSIASWCRFARKNYFYPDMPKNFQISQYDEPLCSNGWLDVEVEGEIHRVGITRVHLEEDTGKSLHVGGATGRIHGA
THSLVDYNRAGIPLVEIVTEPDIRSPEVARAYVDELRELCRALAVSDVRMDQGSLRCDANVSLRLRPAPGEPDAPFGTRS
ETKNLNSLRSVERAVRFEVVRQAALLDAGDRVVQETRHFDEASGRTSSGRSKEEATDYRYFPEPDLTPLALTHEYIEAVR
AVLPELPAQRRVRLIAEHGYTVRDIQEMRNAGVLDLVEATVAAGASPPAARKWWLNELARRAADAGVEPRELAIGPADVA
RICELVAAGQLTDALGRKVIDGVLAGDGSADEVIAARDLAVVSDDSALRDAVDTAIAGAPDIAEKVRGGKVNAVGPLVGA
VMKAMRGQADAAAVRRLLLERLEAS

Sequences:

>Translated_505_residues
MSTSAPVIATPSYEDATARYESVIGLETHVELGTASKMFCGCATAFGAEPNTQVCPVCLGLPGALPVVNEAAVRFATLIG
LALNCSIASWCRFARKNYFYPDMPKNFQISQYDEPLCSNGWLDVEVEGEIHRVGITRVHLEEDTGKSLHVGGATGRIHGA
THSLVDYNRAGIPLVEIVTEPDIRSPEVARAYVDELRELCRALAVSDVRMDQGSLRCDANVSLRLRPAPGEPDAPFGTRS
ETKNLNSLRSVERAVRFEVVRQAALLDAGDRVVQETRHFDEASGRTSSGRSKEEATDYRYFPEPDLTPLALTHEYIEAVR
AVLPELPAQRRVRLIAEHGYTVRDIQEMRNAGVLDLVEATVAAGASPPAARKWWLNELARRAADAGVEPRELAIGPADVA
RICELVAAGQLTDALGRKVIDGVLAGDGSADEVIAARDLAVVSDDSALRDAVDTAIAGAPDIAEKVRGGKVNAVGPLVGA
VMKAMRGQADAAAVRRLLLERLEAS
>Mature_504_residues
STSAPVIATPSYEDATARYESVIGLETHVELGTASKMFCGCATAFGAEPNTQVCPVCLGLPGALPVVNEAAVRFATLIGL
ALNCSIASWCRFARKNYFYPDMPKNFQISQYDEPLCSNGWLDVEVEGEIHRVGITRVHLEEDTGKSLHVGGATGRIHGAT
HSLVDYNRAGIPLVEIVTEPDIRSPEVARAYVDELRELCRALAVSDVRMDQGSLRCDANVSLRLRPAPGEPDAPFGTRSE
TKNLNSLRSVERAVRFEVVRQAALLDAGDRVVQETRHFDEASGRTSSGRSKEEATDYRYFPEPDLTPLALTHEYIEAVRA
VLPELPAQRRVRLIAEHGYTVRDIQEMRNAGVLDLVEATVAAGASPPAARKWWLNELARRAADAGVEPRELAIGPADVAR
ICELVAAGQLTDALGRKVIDGVLAGDGSADEVIAARDLAVVSDDSALRDAVDTAIAGAPDIAEKVRGGKVNAVGPLVGAV
MKAMRGQADAAAVRRLLLERLEAS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=507, Percent_Identity=32.1499013806706, Blast_Score=265, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI115534094, Length=325, Percent_Identity=38.7692307692308, Blast_Score=199, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=525, Percent_Identity=26.8571428571429, Blast_Score=183, Evalue=7e-47,
Organism=Drosophila melanogaster, GI45550808, Length=505, Percent_Identity=30.6930693069307, Blast_Score=231, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54276; Mature: 54145

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00097 CARBAMOYLTRANSFERASE ; PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSAPVIATPSYEDATARYESVIGLETHVELGTASKMFCGCATAFGAEPNTQVCPVCLG
CCCCCCEEECCCCCHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHCCCCCCCCCCHHEEC
LPGALPVVNEAAVRFATLIGLALNCSIASWCRFARKNYFYPDMPKNFQISQYDEPLCSNG
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
WLDVEVEGEIHRVGITRVHLEEDTGKSLHVGGATGRIHGATHSLVDYNRAGIPLVEIVTE
CEEEEECCCEEEEEEEEEEEECCCCCEEEECCCCCEECCCHHHHHHCCCCCCCEEEEECC
PDIRSPEVARAYVDELRELCRALAVSDVRMDQGSLRCDANVSLRLRPAPGEPDAPFGTRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCEEEEECCCCCCCCCCCCCH
ETKNLNSLRSVERAVRFEVVRQAALLDAGDRVVQETRHFDEASGRTSSGRSKEEATDYRY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
FPEPDLTPLALTHEYIEAVRAVLPELPAQRRVRLIAEHGYTVRDIQEMRNAGVLDLVEAT
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
VAAGASPPAARKWWLNELARRAADAGVEPRELAIGPADVARICELVAAGQLTDALGRKVI
HHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHH
DGVLAGDGSADEVIAARDLAVVSDDSALRDAVDTAIAGAPDIAEKVRGGKVNAVGPLVGA
HHHHCCCCCCHHHHHHHCEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
VMKAMRGQADAAAVRRLLLERLEAS
HHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSAPVIATPSYEDATARYESVIGLETHVELGTASKMFCGCATAFGAEPNTQVCPVCLG
CCCCCEEECCCCCHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHCCCCCCCCCCHHEEC
LPGALPVVNEAAVRFATLIGLALNCSIASWCRFARKNYFYPDMPKNFQISQYDEPLCSNG
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
WLDVEVEGEIHRVGITRVHLEEDTGKSLHVGGATGRIHGATHSLVDYNRAGIPLVEIVTE
CEEEEECCCEEEEEEEEEEEECCCCCEEEECCCCCEECCCHHHHHHCCCCCCCEEEEECC
PDIRSPEVARAYVDELRELCRALAVSDVRMDQGSLRCDANVSLRLRPAPGEPDAPFGTRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCEEEEECCCCCCCCCCCCCH
ETKNLNSLRSVERAVRFEVVRQAALLDAGDRVVQETRHFDEASGRTSSGRSKEEATDYRY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
FPEPDLTPLALTHEYIEAVRAVLPELPAQRRVRLIAEHGYTVRDIQEMRNAGVLDLVEAT
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
VAAGASPPAARKWWLNELARRAADAGVEPRELAIGPADVARICELVAAGQLTDALGRKVI
HHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHH
DGVLAGDGSADEVIAARDLAVVSDDSALRDAVDTAIAGAPDIAEKVRGGKVNAVGPLVGA
HHHHCCCCCCHHHHHHHCEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
VMKAMRGQADAAAVRRLLLERLEAS
HHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA