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The map label for this gene is gatA [H]

Identifier: 158312939

GI number: 158312939

Start: 1294441

End: 1295997

Strand: Direct

Name: gatA [H]

Synonym: Franean1_1085

Alternate gene names: 158312939

Gene position: 1294441-1295997 (Clockwise)

Preceding gene: 158312938

Following gene: 158312940

Centisome position: 14.41

GC content: 74.89

Gene sequence:

>1557_bases
ATGACCGACGTTTCCAGCACCTCAACGCCTGCCGGCGCGGCCGCCGCCGGCGCGGGCGCCCCCCGCCGCACGGACGACGT
CGTCCGGCTGACCGCCGCCGAGACGGCGGCCGCGATCGCCGCGGGCGAGCTGTCCGCGCAGGACGCGACCCTCGCCGCGC
TCGACCGCATCGCCAAGGTCGACGAGGCCGTCCACGCCTTCCTGCATGTTGACGCCGAGGGCGCGCTCGCCGCCGCCCGC
GCGATCGACGCCCGCCGCGCCGCCGGGGAGAAGCTCGGCCCGCTCGCCGGCGTCCCGCTCGCGCTCAAGGACGTGCTCAC
CGCGCGGGGCATGCCGACGACCTGCGGCAGCCGCATCCTGGAGGGCTGGCGGCCGCCGTACGACGCGACCGTGGTCTCCC
GGTTGCGCGCCGCCGACGTGGTCATCCTCGGCAAGGTCAACATGGACGAGTTCGCGATGGGCTCGTCCACCGAGAACTCG
GCGTACGGGCCGACCCGCAACCCGTGGGATCTCACCCGCATCCCCGGCGGCAGCAGCGGTGGCAGCGCGGCGGCGGTCGC
TGCCTTCGAAGCGCCGCTGTCCATCGGCACCGACACCGGCGGGTCCATCCGCCAGCCGGCAGCGGTGTGCGGGCTGGTGG
GGGTCAAGCCGACCTACGGCGGGGTGAGCCGCTACGGCCTGGTCGCGTTCTCCAGCTCGCTCGACCAGGCCGGGCCGCTG
GCCCGCACCGTCGGCGACGCCGCCCTGCTGCACGCCGCGATCGCCGGGCACGACCCGCTGGACAGCACCAGCGTCGACCT
GCCGGTGCCGCCGGTGGTCGAGGCCGCCGCGCGCCGCGACATCCGCGGCCTGCGGGTGGGCGTGGTGCGTGAGCTCTCCG
GTGAGGGCTACGACCCGGGTGTGCAGGCGGCGTTCGACGCGGCGGTCCAGGAGCTGGCCGGTCTCGGCGCCGAGGTCGTG
GAGGTGAGCTGCCCGCACTTCACCTACGCGCTGGCGGCGTACTACCTGATCGCGCCGAGCGAGTGCTCGTCCAACCTGGC
CCGGTTCGACGCGATGCGGTTCGGGCTGCGCGCCGGGGACGACGGCCGGGCCGGTGCCGAGGAGGTCATGAGCCACACCC
GCGACGTCGGCTTCGGTGCCGAGGTCAAGCGGCGGGTCATGCTCGGCACGTACGCGCTGTCCTCGGGCTACTACGACGCC
TACTACGGGCAGGCGCAGAAGGTCCGGACGCTGATCAGCCGCGACTTCGCCGCCGCCTACGAGAAGGCCGACGTGCTGGT
CTCGCCGACCACGCCGACGCCGGCCTTCCCGCTCGGCGACAAGGTGGACGACCCGGTGTCGATGTACCTGTCGGACCTGT
GCACGATCCCGTCCAACCTGGCGGGCGGGCCGGCGATGAGCCTGCCGGCCGGCCTGGTGGGCGGGCTGCCCGCCGGCATC
CAGATCATGGCCCCGGCGTTCGCGGACGACCGGCTCTACCTGGTCGGCGGGGCGCTCGAGGCCGCCCTGGAGGCGCGCCG
CGGACATCCGCTGCTGGACGAGGCACCCGCGCTCTAG

Upstream 100 bases:

>100_bases
CGGTCGCCGACGTCCTCGCCGGTGCGCCCGCCGCCGAGGAGGGCCGGTTCCGGGTGCCGCGCATTCTCGATCCGGACGAG
TGAGGGGCACCAGGCCGCAC

Downstream 100 bases:

>100_bases
CGCCGGCACTCGGCACTCGGCACCCGGAACACCGGGAAGCAGGGGAAGCCGGGAAGCCGGGGAAGGTCGGGAAAGTAAGG
ACGAGGAGGGACATGGTCGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTDVSSTSTPAGAAAAGAGAPRRTDDVVRLTAAETAAAIAAGELSAQDATLAALDRIAKVDEAVHAFLHVDAEGALAAAR
AIDARRAAGEKLGPLAGVPLALKDVLTARGMPTTCGSRILEGWRPPYDATVVSRLRAADVVILGKVNMDEFAMGSSTENS
AYGPTRNPWDLTRIPGGSSGGSAAAVAAFEAPLSIGTDTGGSIRQPAAVCGLVGVKPTYGGVSRYGLVAFSSSLDQAGPL
ARTVGDAALLHAAIAGHDPLDSTSVDLPVPPVVEAAARRDIRGLRVGVVRELSGEGYDPGVQAAFDAAVQELAGLGAEVV
EVSCPHFTYALAAYYLIAPSECSSNLARFDAMRFGLRAGDDGRAGAEEVMSHTRDVGFGAEVKRRVMLGTYALSSGYYDA
YYGQAQKVRTLISRDFAAAYEKADVLVSPTTPTPAFPLGDKVDDPVSMYLSDLCTIPSNLAGGPAMSLPAGLVGGLPAGI
QIMAPAFADDRLYLVGGALEAALEARRGHPLLDEAPAL

Sequences:

>Translated_518_residues
MTDVSSTSTPAGAAAAGAGAPRRTDDVVRLTAAETAAAIAAGELSAQDATLAALDRIAKVDEAVHAFLHVDAEGALAAAR
AIDARRAAGEKLGPLAGVPLALKDVLTARGMPTTCGSRILEGWRPPYDATVVSRLRAADVVILGKVNMDEFAMGSSTENS
AYGPTRNPWDLTRIPGGSSGGSAAAVAAFEAPLSIGTDTGGSIRQPAAVCGLVGVKPTYGGVSRYGLVAFSSSLDQAGPL
ARTVGDAALLHAAIAGHDPLDSTSVDLPVPPVVEAAARRDIRGLRVGVVRELSGEGYDPGVQAAFDAAVQELAGLGAEVV
EVSCPHFTYALAAYYLIAPSECSSNLARFDAMRFGLRAGDDGRAGAEEVMSHTRDVGFGAEVKRRVMLGTYALSSGYYDA
YYGQAQKVRTLISRDFAAAYEKADVLVSPTTPTPAFPLGDKVDDPVSMYLSDLCTIPSNLAGGPAMSLPAGLVGGLPAGI
QIMAPAFADDRLYLVGGALEAALEARRGHPLLDEAPAL
>Mature_517_residues
TDVSSTSTPAGAAAAGAGAPRRTDDVVRLTAAETAAAIAAGELSAQDATLAALDRIAKVDEAVHAFLHVDAEGALAAARA
IDARRAAGEKLGPLAGVPLALKDVLTARGMPTTCGSRILEGWRPPYDATVVSRLRAADVVILGKVNMDEFAMGSSTENSA
YGPTRNPWDLTRIPGGSSGGSAAAVAAFEAPLSIGTDTGGSIRQPAAVCGLVGVKPTYGGVSRYGLVAFSSSLDQAGPLA
RTVGDAALLHAAIAGHDPLDSTSVDLPVPPVVEAAARRDIRGLRVGVVRELSGEGYDPGVQAAFDAAVQELAGLGAEVVE
VSCPHFTYALAAYYLIAPSECSSNLARFDAMRFGLRAGDDGRAGAEEVMSHTRDVGFGAEVKRRVMLGTYALSSGYYDAY
YGQAQKVRTLISRDFAAAYEKADVLVSPTTPTPAFPLGDKVDDPVSMYLSDLCTIPSNLAGGPAMSLPAGLVGGLPAGIQ
IMAPAFADDRLYLVGGALEAALEARRGHPLLDEAPAL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=37.7690802348337, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=24.9496981891348, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI166795287, Length=236, Percent_Identity=33.0508474576271, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=418, Percent_Identity=36.3636363636364, Blast_Score=238, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=24.9003984063745, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=295, Percent_Identity=28.8135593220339, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=250, Percent_Identity=32.8, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=300, Percent_Identity=28.3333333333333, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=309, Percent_Identity=28.8025889967638, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=309, Percent_Identity=28.8025889967638, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=317, Percent_Identity=26.4984227129338, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=459, Percent_Identity=32.8976034858388, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=404, Percent_Identity=31.6831683168317, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=241, Percent_Identity=31.1203319502075, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648113, Length=493, Percent_Identity=37.3225152129817, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24652985, Length=517, Percent_Identity=27.4661508704062, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922090, Length=517, Percent_Identity=27.4661508704062, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652981, Length=517, Percent_Identity=27.4661508704062, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652983, Length=517, Percent_Identity=27.4661508704062, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=25.7425742574257, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI45550774, Length=207, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648435, Length=207, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648437, Length=207, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356731, Length=227, Percent_Identity=33.4801762114537, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648441, Length=137, Percent_Identity=38.6861313868613, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=137, Percent_Identity=38.6861313868613, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=25.8893280632411, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53060; Mature: 52929

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVSSTSTPAGAAAAGAGAPRRTDDVVRLTAAETAAAIAAGELSAQDATLAALDRIAKV
CCCCCCCCCCCCCHHCCCCCCCCCCHHEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEAVHAFLHVDAEGALAAARAIDARRAAGEKLGPLAGVPLALKDVLTARGMPTTCGSRIL
HHHHHHHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
EGWRPPYDATVVSRLRAADVVILGKVNMDEFAMGSSTENSAYGPTRNPWDLTRIPGGSSG
HCCCCCCCHHHHHHHHHCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSAAAVAAFEAPLSIGTDTGGSIRQPAAVCGLVGVKPTYGGVSRYGLVAFSSSLDQAGPL
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCH
ARTVGDAALLHAAIAGHDPLDSTSVDLPVPPVVEAAARRDIRGLRVGVVRELSGEGYDPG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VQAAFDAAVQELAGLGAEVVEVSCPHFTYALAAYYLIAPSECSSNLARFDAMRFGLRAGD
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DGRAGAEEVMSHTRDVGFGAEVKRRVMLGTYALSSGYYDAYYGQAQKVRTLISRDFAAAY
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHH
EKADVLVSPTTPTPAFPLGDKVDDPVSMYLSDLCTIPSNLAGGPAMSLPAGLVGGLPAGI
HCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCE
QIMAPAFADDRLYLVGGALEAALEARRGHPLLDEAPAL
EEEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TDVSSTSTPAGAAAAGAGAPRRTDDVVRLTAAETAAAIAAGELSAQDATLAALDRIAKV
CCCCCCCCCCCCHHCCCCCCCCCCHHEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEAVHAFLHVDAEGALAAARAIDARRAAGEKLGPLAGVPLALKDVLTARGMPTTCGSRIL
HHHHHHHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
EGWRPPYDATVVSRLRAADVVILGKVNMDEFAMGSSTENSAYGPTRNPWDLTRIPGGSSG
HCCCCCCCHHHHHHHHHCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSAAAVAAFEAPLSIGTDTGGSIRQPAAVCGLVGVKPTYGGVSRYGLVAFSSSLDQAGPL
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCH
ARTVGDAALLHAAIAGHDPLDSTSVDLPVPPVVEAAARRDIRGLRVGVVRELSGEGYDPG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VQAAFDAAVQELAGLGAEVVEVSCPHFTYALAAYYLIAPSECSSNLARFDAMRFGLRAGD
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DGRAGAEEVMSHTRDVGFGAEVKRRVMLGTYALSSGYYDAYYGQAQKVRTLISRDFAAAY
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHH
EKADVLVSPTTPTPAFPLGDKVDDPVSMYLSDLCTIPSNLAGGPAMSLPAGLVGGLPAGI
HCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCE
QIMAPAFADDRLYLVGGALEAALEARRGHPLLDEAPAL
EEEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA