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The map label for this gene is prfA

Identifier: 158312864

GI number: 158312864

Start: 1192412

End: 1193482

Strand: Direct

Name: prfA

Synonym: Franean1_1010

Alternate gene names: 158312864

Gene position: 1192412-1193482 (Clockwise)

Preceding gene: 158312863

Following gene: 158312865

Centisome position: 13.28

GC content: 72.83

Gene sequence:

>1071_bases
ATGACCTCCCCGCTCGCGGGCATGATCGCCGAGCACGCCGACATCGAGAAGCGGCTCGCGGATCCGGACATCCACAACGA
TCCGGCGCGGGCCCGGGAGCTGACGCGCCGGTACGCCGAGCTCGCCACCCCCGTCGACCTCGCACAGCGGCTGGAGCGCG
TCGAGGGTGACCTGGAGACCGCCCGCGAGTTCGCCGAGCAGGACCCGTCGTTCCGCGACGAGGTCGTGGCCCTCGAGGCG
AGCCAGGCCGATCTCGCCGCCCGCCTGCGGGCCTACCTGGTGCCCGTCGACCCCGACGACAGCCGGGACGCCATCCTCGA
GGTCAAGGCCGGCGCGGGCGGTGAGGAGTCCGCGCTGTTCGCGGGCGACCTGCTGCGCATGTACCTGCGCTACGCCGAGC
GGCGCGGGTGGCGCACCCAGATCCTGGACGCGAACCCCAGCGACCTGGGCGGCTACCGGGACGTCAGTGTGGCCGTCAAG
TCCCGCGGGACGGCGGCCCCCGGCCAGGGGGTCTTCGGCCGGCTGCGGTTCGAGGGCGGTGTGCACCGGGTGCAGCGGGT
GCCGGTGACCGAGTCGGCGGGGCGGATCCACACCTCCGCGGCCGGGGTGCTGGTGCTGCCCGAGGCAGCCGACGTCGACA
TCGAAGTCGACCCGAACGATCTGCGGATCGACGTGTTCCGCTCGTCCGGGCCGGGCGGCCAGAGCGTCAACACCACCGAC
TCGGCGGTGCGCATCACGCACCTGCCCACAGGGGTGGTCGTCTCCTGCCAGAACGAGAAGAGCCAGCTCCAGAACAAGGA
GTCGGCGATGCGCATCCTGCGGGCCCGGCTGCTCGCCGCGGCGCGGGAGAAGGCCGAGACGGCGGCGGCGGCGGCGCGGG
CGAGCCAGGTGCGCACGGTCGACCGCTCGGAGAAGGTCCGCACCTACAACTTCCCCGAGAACCGGATCAGCGACCACCGC
ATCGGCTACAAGGCGCACAACCTGGAGGCCGTGCTGGACGGCGACCTCGACGCCGTGATCGAGGCCCTGACCGAGGCGGA
CCTGGAATCCCGGATGGCGGCCACGCCGTGA

Upstream 100 bases:

>100_bases
GGGCCAGAAGGTCGGCGGCGCGAAGTAGGTCGTCCTCGGCGCCCGTCCCGGATCCCCGGGCGGGCGCCGTTCGCGTCCCG
ACGACCTGAAGGAACCCCTG

Downstream 100 bases:

>100_bases
CAGGCCCGGCCGCGTCCACGACGCGGGCCGACCACGCGGACGCCACGCTCGCCGTCGAGCTCGCCGCCGCGACGGCCCGG
CTGGCCGCGGCCGGTGTCGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTSPLAGMIAEHADIEKRLADPDIHNDPARARELTRRYAELATPVDLAQRLERVEGDLETAREFAEQDPSFRDEVVALEA
SQADLAARLRAYLVPVDPDDSRDAILEVKAGAGGEESALFAGDLLRMYLRYAERRGWRTQILDANPSDLGGYRDVSVAVK
SRGTAAPGQGVFGRLRFEGGVHRVQRVPVTESAGRIHTSAAGVLVLPEAADVDIEVDPNDLRIDVFRSSGPGGQSVNTTD
SAVRITHLPTGVVVSCQNEKSQLQNKESAMRILRARLLAAAREKAETAAAAARASQVRTVDRSEKVRTYNFPENRISDHR
IGYKAHNLEAVLDGDLDAVIEALTEADLESRMAATP

Sequences:

>Translated_356_residues
MTSPLAGMIAEHADIEKRLADPDIHNDPARARELTRRYAELATPVDLAQRLERVEGDLETAREFAEQDPSFRDEVVALEA
SQADLAARLRAYLVPVDPDDSRDAILEVKAGAGGEESALFAGDLLRMYLRYAERRGWRTQILDANPSDLGGYRDVSVAVK
SRGTAAPGQGVFGRLRFEGGVHRVQRVPVTESAGRIHTSAAGVLVLPEAADVDIEVDPNDLRIDVFRSSGPGGQSVNTTD
SAVRITHLPTGVVVSCQNEKSQLQNKESAMRILRARLLAAAREKAETAAAAARASQVRTVDRSEKVRTYNFPENRISDHR
IGYKAHNLEAVLDGDLDAVIEALTEADLESRMAATP
>Mature_355_residues
TSPLAGMIAEHADIEKRLADPDIHNDPARARELTRRYAELATPVDLAQRLERVEGDLETAREFAEQDPSFRDEVVALEAS
QADLAARLRAYLVPVDPDDSRDAILEVKAGAGGEESALFAGDLLRMYLRYAERRGWRTQILDANPSDLGGYRDVSVAVKS
RGTAAPGQGVFGRLRFEGGVHRVQRVPVTESAGRIHTSAAGVLVLPEAADVDIEVDPNDLRIDVFRSSGPGGQSVNTTDS
AVRITHLPTGVVVSCQNEKSQLQNKESAMRILRARLLAAAREKAETAAAAARASQVRTVDRSEKVRTYNFPENRISDHRI
GYKAHNLEAVLDGDLDAVIEALTEADLESRMAATP

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=281, Percent_Identity=46.6192170818505, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI34577120, Length=362, Percent_Identity=38.121546961326, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=44.9438202247191, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=45.6824512534819, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=40, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=296, Percent_Identity=37.1621621621622, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=367, Percent_Identity=40.3269754768392, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921226, Length=321, Percent_Identity=41.1214953271028, Blast_Score=231, Evalue=5e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_FRASN (A8L3V1)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505372.1
- GeneID:   5669424
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1010
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   FSP1855:FRANEAN1_1010-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38751; Mature: 38620

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPLAGMIAEHADIEKRLADPDIHNDPARARELTRRYAELATPVDLAQRLERVEGDLET
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHH
AREFAEQDPSFRDEVVALEASQADLAARLRAYLVPVDPDDSRDAILEVKAGAGGEESALF
HHHHHHCCCCCCHHEEEEECCHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCCCHHH
AGDLLRMYLRYAERRGWRTQILDANPSDLGGYRDVSVAVKSRGTAAPGQGVFGRLRFEGG
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCC
VHRVQRVPVTESAGRIHTSAAGVLVLPEAADVDIEVDPNDLRIDVFRSSGPGGQSVNTTD
HHHHEECCCCCCCCCEEECCCCEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGVVVSCQNEKSQLQNKESAMRILRARLLAAAREKAETAAAAARASQVRTV
CEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DRSEKVRTYNFPENRISDHRIGYKAHNLEAVLDGDLDAVIEALTEADLESRMAATP
CCCCCCEEECCCHHHCCCCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSPLAGMIAEHADIEKRLADPDIHNDPARARELTRRYAELATPVDLAQRLERVEGDLET
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHH
AREFAEQDPSFRDEVVALEASQADLAARLRAYLVPVDPDDSRDAILEVKAGAGGEESALF
HHHHHHCCCCCCHHEEEEECCHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCCCHHH
AGDLLRMYLRYAERRGWRTQILDANPSDLGGYRDVSVAVKSRGTAAPGQGVFGRLRFEGG
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCC
VHRVQRVPVTESAGRIHTSAAGVLVLPEAADVDIEVDPNDLRIDVFRSSGPGGQSVNTTD
HHHHEECCCCCCCCCEEECCCCEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGVVVSCQNEKSQLQNKESAMRILRARLLAAAREKAETAAAAARASQVRTV
CEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DRSEKVRTYNFPENRISDHRIGYKAHNLEAVLDGDLDAVIEALTEADLESRMAATP
CCCCCCEEECCCHHHCCCCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA