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The map label for this gene is rpoE [H]

Identifier: 158312815

GI number: 158312815

Start: 1125172

End: 1125984

Strand: Direct

Name: rpoE [H]

Synonym: Franean1_0961

Alternate gene names: 158312815

Gene position: 1125172-1125984 (Clockwise)

Preceding gene: 158312814

Following gene: 158312816

Centisome position: 12.53

GC content: 69.5

Gene sequence:

>813_bases
ATGACGTTCCAGCACAGCACACCAGGCCCGCGCTCGCAGGATCGCCGGGTGACGGGGCCGGGCCGCGGGCGGCCGGTCGC
CGCGCCGGTGCGCACCAGGGCGGTGGCGGGGGTATCGTCCGGCGCGGAGAGCGGTCCAGTCAGGACGGCAGCCGGCGGGC
CGGCGATCGGTTCCCGGCGGGGCGAAGGAGTGATCCTGGTCGACGAGACCAATGACGAGTCCACCCAGGAGCGCGGCGCC
CGGTTCGAGCGGGATGCCCTGCCGTTCCTCGACCAGCTCTACGCTGCCGCGCTGCGGATGACCCGCAACCCCGCGGACGC
GGAGGATCTCGTTCAGGAGACCTTCGTCAAGGCATTCGCGGCCTTCCACCAGTTCCAGGAGGGAACCAACCTCAAGGCCT
GGTTGTACCGCATCCTGACGAACACGTTCATCAACACGTACCGCAAGCGCCAGCGGCAGCCGCTGCAGAGCCCGACCGAG
CAGGTCGAGGACTGGCAGCTCGCCGAGGCGGAGTCGCACACGTCCACCGGCCTGAAGAGCGCCGAGGCCGAGGCGTTGGA
GCACCTGCCCGACAGCGACATCACCGAGGCGCTCCAGGCGCTGCCCGAGGACTTCCGGATGGCGGTCTATCTGGCCGACG
TCGAGGGGTTCTCGTACAAGGAGATCGCTGAGATCATGGGCACTCCCATCGGTACCGTGATGTCACGGCTGCACCGTGGG
CGGCGTGGTCTGCGAAAGTCGCTGGAGCAGTACGCGCTGGAGCGCGGGCTGGTTCCGGCCGGGTCTGCCGAGAACGAGGG
CGGTGGGTCGTGA

Upstream 100 bases:

>100_bases
CCGTGGTGGTTGGCGCCAGTGGGAAGCGCGGGAGGCCAGGCGTGGCCGGTGTCGCACGGAGGTGGTGCGACCGCCGGAGT
GCCCGCGTCACGGAGGTTCG

Downstream 100 bases:

>100_bases
GCTGCGGAGGACCTCACAACATCGATTGCCGCAAGGTGCTCGACGCGGTCTTTCTTTACCTCGACGGGGAGTGCAACGAC
CCGCAGCAGCACCTGATCCG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTFQHSTPGPRSQDRRVTGPGRGRPVAAPVRTRAVAGVSSGAESGPVRTAAGGPAIGSRRGEGVILVDETNDESTQERGA
RFERDALPFLDQLYAAALRMTRNPADAEDLVQETFVKAFAAFHQFQEGTNLKAWLYRILTNTFINTYRKRQRQPLQSPTE
QVEDWQLAEAESHTSTGLKSAEAEALEHLPDSDITEALQALPEDFRMAVYLADVEGFSYKEIAEIMGTPIGTVMSRLHRG
RRGLRKSLEQYALERGLVPAGSAENEGGGS

Sequences:

>Translated_270_residues
MTFQHSTPGPRSQDRRVTGPGRGRPVAAPVRTRAVAGVSSGAESGPVRTAAGGPAIGSRRGEGVILVDETNDESTQERGA
RFERDALPFLDQLYAAALRMTRNPADAEDLVQETFVKAFAAFHQFQEGTNLKAWLYRILTNTFINTYRKRQRQPLQSPTE
QVEDWQLAEAESHTSTGLKSAEAEALEHLPDSDITEALQALPEDFRMAVYLADVEGFSYKEIAEIMGTPIGTVMSRLHRG
RRGLRKSLEQYALERGLVPAGSAENEGGGS
>Mature_269_residues
TFQHSTPGPRSQDRRVTGPGRGRPVAAPVRTRAVAGVSSGAESGPVRTAAGGPAIGSRRGEGVILVDETNDESTQERGAR
FERDALPFLDQLYAAALRMTRNPADAEDLVQETFVKAFAAFHQFQEGTNLKAWLYRILTNTFINTYRKRQRQPLQSPTEQ
VEDWQLAEAESHTSTGLKSAEAEALEHLPDSDITEALQALPEDFRMAVYLADVEGFSYKEIAEIMGTPIGTVMSRLHRGR
RGLRKSLEQYALERGLVPAGSAENEGGGS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=148, Percent_Identity=35.1351351351351, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 29541; Mature: 29410

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQHSTPGPRSQDRRVTGPGRGRPVAAPVRTRAVAGVSSGAESGPVRTAAGGPAIGSRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GEGVILVDETNDESTQERGARFERDALPFLDQLYAAALRMTRNPADAEDLVQETFVKAFA
CCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AFHQFQEGTNLKAWLYRILTNTFINTYRKRQRQPLQSPTEQVEDWQLAEAESHTSTGLKS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCHH
AEAEALEHLPDSDITEALQALPEDFRMAVYLADVEGFSYKEIAEIMGTPIGTVMSRLHRG
HHHHHHHHCCCCHHHHHHHHCCHHHHEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHH
RRGLRKSLEQYALERGLVPAGSAENEGGGS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFQHSTPGPRSQDRRVTGPGRGRPVAAPVRTRAVAGVSSGAESGPVRTAAGGPAIGSRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GEGVILVDETNDESTQERGARFERDALPFLDQLYAAALRMTRNPADAEDLVQETFVKAFA
CCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AFHQFQEGTNLKAWLYRILTNTFINTYRKRQRQPLQSPTEQVEDWQLAEAESHTSTGLKS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCHH
AEAEALEHLPDSDITEALQALPEDFRMAVYLADVEGFSYKEIAEIMGTPIGTVMSRLHRG
HHHHHHHHCCCCHHHHHHHHCCHHHHEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHH
RRGLRKSLEQYALERGLVPAGSAENEGGGS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]