Click here to switch to the map view.

The map label for this gene is hemL [H]

Identifier: 158312803

GI number: 158312803

Start: 1111392

End: 1112813

Strand: Direct

Name: hemL [H]

Synonym: Franean1_0949

Alternate gene names: 158312803

Gene position: 1111392-1112813 (Clockwise)

Preceding gene: 158312799

Following gene: 158312805

Centisome position: 12.37

GC content: 71.31

Gene sequence:

>1422_bases
GTGACGGTGTCCAACGCGACGACCACGACTCCTCGTCCCGGTGACCCGCCGCGGACTGGGCACGCCGGGCGGGTGACCGA
TCTCCTCGAGCGGGAGGAGCGCGCGCTGCAGGCCCGCACTCCGGCGTCCGAGGCCATGCACACCCGTGCCCTGCGGACGA
TGACCGGCGGGGTCCCGTCCTCCTACCAGCTGCGCGACCCCTGGCCGATCTACCTCACGCACGGCCGCGGCTCGCTGGTC
TGGGACGTCGACGGCAACGAGTACTCCGACTTCCACAACGGGTACGGCTCGATGGTTCAGGGCCACGCCCACCCCGCGAT
CGTGCGCGCGGTGACCGAGCGGATGGCGCTCGGCTCGCACTTCGCCATGCCCACCGAGGATTCGGTGCTGGTCAGCGAGG
AGCTGGCCCGCCGCTTCGGGCTGCCGCAGTGGCGTTACGTCAACTCCGGCTCCGAGGCGACCATGGACGCCATCCGCATC
GCCCGCGGGGTGACCGGCCGGGACACCATCGTCAAGATCTTCGGCTCGTACCACGGCCATCACGACTACGTGATGGTCTC
GATCGGCACCCCCTACGGGGACATCGGGCCGGCGGACCATATGAACTCCCTGGCCTACGGCGCGGGAATCCCGCAGGCGG
TGGTCGACCTGACGGTGCCCGTGCCGTTCAACGACGCGGCGGCGATGGAACGGCGGATCGCCGCGCTCGAGGCCGAGGGG
CGCAAGCCCGCCTGTGTGATCATGGAGGCGGCGATGATGAACCTCGGCGTCGTCCTGCCCGAGCCGGGCTACCTGGAGGC
CGTCCGGGAGATCACACGCAGGCACGGCATCGTGCTCATCTTCGACGAGGTCAAGACCGGGCTGTGCGTCGCGGCCGGCG
GCGCCGTCGAGCGGTTCGGGGTGCTGCCCGACATGGTCACCCTCGCCAAGGCGCTCGGCGGCGGACTGCCGGCCGGCGCG
ATCGGCGCCACCGCGGAGCTGATGGCCGCGGTCGCCGAGGACAGGGTGAAACAGGTGGGGACCTTCAACGGCAACCCGCT
GGTCATGGCGGCGGCGCGGGCCAGCCTGACCGAGGTCCTGACCCCGGACGCCTACGCCCACCTCGACCGCCTCAACGACC
GCCTGGTGGACGGCTGCACCGCGATCCTGGCCCGCCACGGCATCGCCGGCTACGCCGTCGGGATCAGCTCGAAGGGATGC
GTCCACTTCACCGACGCCCCGATCCGGGACTACACGTCCTTCATGGCCCACCAGAACGCGGTCCTGCCGGAGCTGGCCTG
GCTCTACAACGCCAACCGGCAGGTGCTGATGGCCCCCGGCCGGGAGGAGGAGTGGACGCTGTCCGTCCAGCACACCGACG
CCGACGTCGACCGCTACCTGGCGAGCCTCGACGCGATGGCCGCGGACCTCGCCCGCGGCTGA

Upstream 100 bases:

>100_bases
GCCTGGATATGGCATTTCTACCTCTCCGTCTCCACGGATTCCATCGCAAACAGCGCCCCGGGGCTACGCTTTCGATAGCC
GAGAGCAAGGGAAGGGTGCG

Downstream 100 bases:

>100_bases
CGGGTCCTGCGCCCGCCGGCCGTGAGCGCCCGCCGGCCGTGAGCGCCCGCCGGCCGTCGCGGCCGCTGACTGCCAATGGT
CGCTGACTGTCAGCATTCTC

Product: class III aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTVSNATTTTPRPGDPPRTGHAGRVTDLLEREERALQARTPASEAMHTRALRTMTGGVPSSYQLRDPWPIYLTHGRGSLV
WDVDGNEYSDFHNGYGSMVQGHAHPAIVRAVTERMALGSHFAMPTEDSVLVSEELARRFGLPQWRYVNSGSEATMDAIRI
ARGVTGRDTIVKIFGSYHGHHDYVMVSIGTPYGDIGPADHMNSLAYGAGIPQAVVDLTVPVPFNDAAAMERRIAALEAEG
RKPACVIMEAAMMNLGVVLPEPGYLEAVREITRRHGIVLIFDEVKTGLCVAAGGAVERFGVLPDMVTLAKALGGGLPAGA
IGATAELMAAVAEDRVKQVGTFNGNPLVMAAARASLTEVLTPDAYAHLDRLNDRLVDGCTAILARHGIAGYAVGISSKGC
VHFTDAPIRDYTSFMAHQNAVLPELAWLYNANRQVLMAPGREEEWTLSVQHTDADVDRYLASLDAMAADLARG

Sequences:

>Translated_473_residues
MTVSNATTTTPRPGDPPRTGHAGRVTDLLEREERALQARTPASEAMHTRALRTMTGGVPSSYQLRDPWPIYLTHGRGSLV
WDVDGNEYSDFHNGYGSMVQGHAHPAIVRAVTERMALGSHFAMPTEDSVLVSEELARRFGLPQWRYVNSGSEATMDAIRI
ARGVTGRDTIVKIFGSYHGHHDYVMVSIGTPYGDIGPADHMNSLAYGAGIPQAVVDLTVPVPFNDAAAMERRIAALEAEG
RKPACVIMEAAMMNLGVVLPEPGYLEAVREITRRHGIVLIFDEVKTGLCVAAGGAVERFGVLPDMVTLAKALGGGLPAGA
IGATAELMAAVAEDRVKQVGTFNGNPLVMAAARASLTEVLTPDAYAHLDRLNDRLVDGCTAILARHGIAGYAVGISSKGC
VHFTDAPIRDYTSFMAHQNAVLPELAWLYNANRQVLMAPGREEEWTLSVQHTDADVDRYLASLDAMAADLARG
>Mature_472_residues
TVSNATTTTPRPGDPPRTGHAGRVTDLLEREERALQARTPASEAMHTRALRTMTGGVPSSYQLRDPWPIYLTHGRGSLVW
DVDGNEYSDFHNGYGSMVQGHAHPAIVRAVTERMALGSHFAMPTEDSVLVSEELARRFGLPQWRYVNSGSEATMDAIRIA
RGVTGRDTIVKIFGSYHGHHDYVMVSIGTPYGDIGPADHMNSLAYGAGIPQAVVDLTVPVPFNDAAAMERRIAALEAEGR
KPACVIMEAAMMNLGVVLPEPGYLEAVREITRRHGIVLIFDEVKTGLCVAAGGAVERFGVLPDMVTLAKALGGGLPAGAI
GATAELMAAVAEDRVKQVGTFNGNPLVMAAARASLTEVLTPDAYAHLDRLNDRLVDGCTAILARHGIAGYAVGISSKGCV
HFTDAPIRDYTSFMAHQNAVLPELAWLYNANRQVLMAPGREEEWTLSVQHTDADVDRYLASLDAMAADLARG

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=312, Percent_Identity=31.4102564102564, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI37574042, Length=341, Percent_Identity=26.6862170087977, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI24119277, Length=371, Percent_Identity=25.8760107816712, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI226442709, Length=309, Percent_Identity=27.1844660194175, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI226442705, Length=341, Percent_Identity=26.099706744868, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI284507298, Length=149, Percent_Identity=36.9127516778523, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1786349, Length=422, Percent_Identity=35.0710900473934, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789016, Length=336, Percent_Identity=30.3571428571429, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693181, Length=404, Percent_Identity=28.960396039604, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788044, Length=352, Percent_Identity=30.6818181818182, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789759, Length=318, Percent_Identity=29.2452830188679, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787560, Length=352, Percent_Identity=28.6931818181818, Blast_Score=111, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI25144271, Length=310, Percent_Identity=29.3548387096774, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=366, Percent_Identity=25.6830601092896, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=33.5664335664336, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=311, Percent_Identity=29.2604501607717, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356575, Length=332, Percent_Identity=29.2168674698795, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357415, Length=322, Percent_Identity=28.2608695652174, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 50789; Mature: 50658

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSNATTTTPRPGDPPRTGHAGRVTDLLEREERALQARTPASEAMHTRALRTMTGGVPS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
SYQLRDPWPIYLTHGRGSLVWDVDGNEYSDFHNGYGSMVQGHAHPAIVRAVTERMALGSH
CCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCC
FAMPTEDSVLVSEELARRFGLPQWRYVNSGSEATMDAIRIARGVTGRDTIVKIFGSYHGH
CCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
HDYVMVSIGTPYGDIGPADHMNSLAYGAGIPQAVVDLTVPVPFNDAAAMERRIAALEAEG
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCCC
RKPACVIMEAAMMNLGVVLPEPGYLEAVREITRRHGIVLIFDEVKTGLCVAAGGAVERFG
CCCHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEECCCHHHHHC
VLPDMVTLAKALGGGLPAGAIGATAELMAAVAEDRVKQVGTFNGNPLVMAAARASLTEVL
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHH
TPDAYAHLDRLNDRLVDGCTAILARHGIAGYAVGISSKGCVHFTDAPIRDYTSFMAHQNA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHCCC
VLPELAWLYNANRQVLMAPGREEEWTLSVQHTDADVDRYLASLDAMAADLARG
HHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVSNATTTTPRPGDPPRTGHAGRVTDLLEREERALQARTPASEAMHTRALRTMTGGVPS
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
SYQLRDPWPIYLTHGRGSLVWDVDGNEYSDFHNGYGSMVQGHAHPAIVRAVTERMALGSH
CCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCC
FAMPTEDSVLVSEELARRFGLPQWRYVNSGSEATMDAIRIARGVTGRDTIVKIFGSYHGH
CCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
HDYVMVSIGTPYGDIGPADHMNSLAYGAGIPQAVVDLTVPVPFNDAAAMERRIAALEAEG
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCCC
RKPACVIMEAAMMNLGVVLPEPGYLEAVREITRRHGIVLIFDEVKTGLCVAAGGAVERFG
CCCHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEECCCHHHHHC
VLPDMVTLAKALGGGLPAGAIGATAELMAAVAEDRVKQVGTFNGNPLVMAAARASLTEVL
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHH
TPDAYAHLDRLNDRLVDGCTAILARHGIAGYAVGISSKGCVHFTDAPIRDYTSFMAHQNA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHCCC
VLPELAWLYNANRQVLMAPGREEEWTLSVQHTDADVDRYLASLDAMAADLARG
HHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]