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The map label for this gene is dagK [H]

Identifier: 158312748

GI number: 158312748

Start: 1043618

End: 1044568

Strand: Direct

Name: dagK [H]

Synonym: Franean1_0894

Alternate gene names: 158312748

Gene position: 1043618-1044568 (Clockwise)

Preceding gene: 158312747

Following gene: 158312752

Centisome position: 11.62

GC content: 74.45

Gene sequence:

>951_bases
ATGACCGAGCCGGGCACGGCCAGCAGCAGCACCGCGGCGCGGGCCGAGGTGCCCGACGCGGCGGTGGACCGTTCGAGGCT
CACCGTCGTGGTCAACCCGAAGGCTGGCGGCGGCCGGGCGGCGAAGGTCCTCGACGGCGTCCGCGCGGCGCTCGCCCGCT
GGGCCGAGGACGTCTCCGTCGAGACCACGAAAAGCCTCGAGCACGCCGAGGAACTAGCCCGCTCGGCCGTCGCGGCGGGG
CGGGTGACCGTCGCGCTGGGTGGTGACGGCCTGGTCGGCAGGGTGGCGGGCGCCGTCGCCCGGTCGGGCGGCGTGCTCGC
GGTGCTGCCCGGCGGCCGGGGCAACGACTTCGCGCGAGGACTGGGCATCCCGCGTGATCCGGCACTCGCCGCGACCGCGC
TCGTCGCGGCCGTGGAGCGCAGGGTGGACCTGCCGGAGGCGAACGGGGTGCCGTTCGTCGGGATTGCCAGCCTCGGGTTC
GACTCCGACGTCCAGGTGATCGCGAACCGGACGACCTGGCTGTCCGGCCAGAGCGTCTACACCTACGCGGCGTTGCGCGG
GGTGGCGGCCTGGAAGCCGGCCCGGTTCACCGTGACCATCGACGGCGAGCCGCCACTGGAGCACGTCGGGTGGACGGTCG
GCGCGGCGAACGGTCCGTACTACGGCGGCGGGATGAAGTTCGCCCCGGACGCCGACATCGCCGATGGCCGGTTGGAGATC
GTCCTGGTCGCGCGCACCGGGCGGTTCACCTTCCTTCGGTTGTTCCCGCGCATCTTCTCCGGCCGGCACGTCGAGGTCCC
CTACGTCCAGGTGCGGCGGGGCGAGCGGCTCGTCGTGGACGCGGACCGCCCGTTCCAGGTCTACGCGGACGGCGACCCGA
TCGCCGACCTCCCGGCTGAGATCGTCGTCCGGCCCGGGGCCCTGCGCCTGCTCACGCCGCCCCAGGCCTAA

Upstream 100 bases:

>100_bases
GCCGCCACGGATCAGCCGGGGCCGGCGGTGGGGTCGCGGACGGCTGGTGGCCGTAGGCTTTGCCGCGGCCGGCAGGGCGT
CGGCGGGGAGGGAACGACGG

Downstream 100 bases:

>100_bases
TGACCTGACCTGCCCGTGCCCCGCGGGCGGCCGGCGGGCGAGGGCTAGCGCAGTTCGGGGGTGGCGTGGTAGTCGGTGAC
GCCGGCGACCTCCGGGTAGT

Product: diacylglycerol kinase catalytic protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTEPGTASSSTAARAEVPDAAVDRSRLTVVVNPKAGGGRAAKVLDGVRAALARWAEDVSVETTKSLEHAEELARSAVAAG
RVTVALGGDGLVGRVAGAVARSGGVLAVLPGGRGNDFARGLGIPRDPALAATALVAAVERRVDLPEANGVPFVGIASLGF
DSDVQVIANRTTWLSGQSVYTYAALRGVAAWKPARFTVTIDGEPPLEHVGWTVGAANGPYYGGGMKFAPDADIADGRLEI
VLVARTGRFTFLRLFPRIFSGRHVEVPYVQVRRGERLVVDADRPFQVYADGDPIADLPAEIVVRPGALRLLTPPQA

Sequences:

>Translated_316_residues
MTEPGTASSSTAARAEVPDAAVDRSRLTVVVNPKAGGGRAAKVLDGVRAALARWAEDVSVETTKSLEHAEELARSAVAAG
RVTVALGGDGLVGRVAGAVARSGGVLAVLPGGRGNDFARGLGIPRDPALAATALVAAVERRVDLPEANGVPFVGIASLGF
DSDVQVIANRTTWLSGQSVYTYAALRGVAAWKPARFTVTIDGEPPLEHVGWTVGAANGPYYGGGMKFAPDADIADGRLEI
VLVARTGRFTFLRLFPRIFSGRHVEVPYVQVRRGERLVVDADRPFQVYADGDPIADLPAEIVVRPGALRLLTPPQA
>Mature_315_residues
TEPGTASSSTAARAEVPDAAVDRSRLTVVVNPKAGGGRAAKVLDGVRAALARWAEDVSVETTKSLEHAEELARSAVAAGR
VTVALGGDGLVGRVAGAVARSGGVLAVLPGGRGNDFARGLGIPRDPALAATALVAAVERRVDLPEANGVPFVGIASLGFD
SDVQVIANRTTWLSGQSVYTYAALRGVAAWKPARFTVTIDGEPPLEHVGWTVGAANGPYYGGGMKFAPDADIADGRLEIV
LVARTGRFTFLRLFPRIFSGRHVEVPYVQVRRGERLVVDADRPFQVYADGDPIADLPAEIVVRPGALRLLTPPQA

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=272, Percent_Identity=26.1029411764706, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32973; Mature: 32842

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPGTASSSTAARAEVPDAAVDRSRLTVVVNPKAGGGRAAKVLDGVRAALARWAEDVSV
CCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ETTKSLEHAEELARSAVAAGRVTVALGGDGLVGRVAGAVARSGGVLAVLPGGRGNDFARG
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHC
LGIPRDPALAATALVAAVERRVDLPEANGVPFVGIASLGFDSDVQVIANRTTWLSGQSVY
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEECEEEECCCCEE
TYAALRGVAAWKPARFTVTIDGEPPLEHVGWTVGAANGPYYGGGMKFAPDADIADGRLEI
EHHHHHHHHCCCCEEEEEEECCCCCHHHCCEEEECCCCCEECCCEEECCCCCCCCCEEEE
VLVARTGRFTFLRLFPRIFSGRHVEVPYVQVRRGERLVVDADRPFQVYADGDPIADLPAE
EEEEECCCCHHHHHHHHHHCCCEEECCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCE
IVVRPGALRLLTPPQA
EEECCCEEEEECCCCC
>Mature Secondary Structure 
TEPGTASSSTAARAEVPDAAVDRSRLTVVVNPKAGGGRAAKVLDGVRAALARWAEDVSV
CCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ETTKSLEHAEELARSAVAAGRVTVALGGDGLVGRVAGAVARSGGVLAVLPGGRGNDFARG
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHC
LGIPRDPALAATALVAAVERRVDLPEANGVPFVGIASLGFDSDVQVIANRTTWLSGQSVY
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEECEEEECCCCEE
TYAALRGVAAWKPARFTVTIDGEPPLEHVGWTVGAANGPYYGGGMKFAPDADIADGRLEI
EHHHHHHHHCCCCEEEEEEECCCCCHHHCCEEEECCCCCEECCCEEECCCCCCCCCEEEE
VLVARTGRFTFLRLFPRIFSGRHVEVPYVQVRRGERLVVDADRPFQVYADGDPIADLPAE
EEEEECCCCHHHHHHHHHHCCCEEECCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCE
IVVRPGALRLLTPPQA
EEECCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]