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The map label for this gene is stp [H]

Identifier: 158312527

GI number: 158312527

Start: 792850

End: 794304

Strand: Direct

Name: stp [H]

Synonym: Franean1_0670

Alternate gene names: 158312527

Gene position: 792850-794304 (Clockwise)

Preceding gene: 158312526

Following gene: 158312529

Centisome position: 8.83

GC content: 75.4

Gene sequence:

>1455_bases
GTGGAGATCGCGAGCCGGGCAGGACGGATGTCCGGTGCGGCCGGTGCCCTGCTGCGGGAGCCCGCCCGGCCGGCCAGGAT
CCGTGAGTCGCCGCTGGCGACCGTGCTCGCGGTCGGCACCGTCTGCATCGGCAGCTTCCTGGGCCAGCTCGACGCCAGCA
TCGTCACGATCGCGCTCGCCGACCTGCGTGCCGACTTCGGCATGTCCCTCGGCCTGGCGACCTGGGTCAGCCTTTCCTAC
CTGGTGACGCTGGTCGCGACCATCGCCGCGCTCGGCCGGCTCGCCGACATGTACGGCCGCAAGCTGCTGACCCTGTACGG
ATTCGCCCTGTTCACGGTGGCCTCGGTCGGCGCCGCCCTGGCCCCCGGCATCGGGGTGCTCATCGTCTGCCGGGTCGCGC
AGGCCCTCGGCGCGGCCATGCTGCAGGCGAACAGCGTCGCCCTGATCGCGACGACCGTCGAGCCGAGGCGTCTCACCGCG
GCGCTCGGCGCCCAGGGCGCCGCCCAGGCGTTCGGGCTCTCGCTCGGGCCGTTGGCCGGCGGTCTGCTGCTCGAGCTCGG
CGGGTGGCGGTGGGTCTTCTGGGCCCACATACCCGCGGGCGTGATCGGGTTCGTGCTCGGCGTCCTGTTGCTGCCCCGCA
CGAGGGCGCACGCGCCGCGGACGCCGTTCGACTGGCGGGGCCTGGCCCTCCTGGTGCCCACGGTGTCGGCCGTGCTGCTC
GCGCTGTCGGTCGGCGAGTCCGCCCGCTCGGTCGGTCCGCTCGCCGCCGTCGGCGGGCTGGTGCTGGTCGCGGCGCTCGG
CGGGGTGCTGTTCGTCCGCCGCGAGTCGTCCGCCGCGAGCCCGATGGTCGACCTGCCGCTCGTGCTGCACCGGGCGGTCG
GGCCGCGGCTGGCCGCGGGGACGTTCTCCTTCCTCGTCCTGTTCGGCCTGCTGCTCACGATCCCGCAGAACTCCGACGAC
GGCGCCGTGGCGGTGGGCCTGCTGCTGGCGACCCTGCCGATCGCGATCGGGGTCGCGGCGCCGGTCGCGGGCACGCTCGT
CACCCGGCTCGGCGTCCGCGCGGTGACGTCCGGCGCGATGGTGTGCGTGGCCGGCGGCCTCGGCCTGCTGGCGGCGGGGG
GGAGCGGCCCGGCTGTCGGCGTGCCCTCGCTGGCGCTGATCGGCTGCGGGCTCGGCCTGTTCACCCCGTCCAACAACGGC
GCCGTCATGGCTGCCGTGCCGGCACAGCAGAGCGGGATGGCCAGCGGCGTGGTGAACATGACCCGAGGGCTCGGCACAGC
CCTGGGTGTCTCGGTGTCGGCTCTCGCCTACGCGGCGGGTGGCTTCGTCGCGACGATGCTGCTGCTCGCCGCGATCGCGC
TCGGCGGGGCACTGCTCGCCGCCACAGCCGCCGCGCCCACGGTGCCGGGCAGCGCGCTGCCGCGTCAGCGCGAGAACGAC
CGCGCGGCGCCGTAA

Upstream 100 bases:

>100_bases
GGTCCCGCCCGCCTTCCGTGAGCGGCGCTGCCCGGTGTGTCCGTGTGACTTCGTCCGGTTGCGGTCGACAGATACCTCTG
TTGCAGAGGAATATAAGGGC

Downstream 100 bases:

>100_bases
CCGGGCCCGATCTCCGGCCGGCCGGCGCGGGACGTCCACCGCCACTGCACCGAGACGCGCCCGCCCACGTACCCGGGGAC
CCGGGGCACCGCGTGCAGCC

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MEIASRAGRMSGAAGALLREPARPARIRESPLATVLAVGTVCIGSFLGQLDASIVTIALADLRADFGMSLGLATWVSLSY
LVTLVATIAALGRLADMYGRKLLTLYGFALFTVASVGAALAPGIGVLIVCRVAQALGAAMLQANSVALIATTVEPRRLTA
ALGAQGAAQAFGLSLGPLAGGLLLELGGWRWVFWAHIPAGVIGFVLGVLLLPRTRAHAPRTPFDWRGLALLVPTVSAVLL
ALSVGESARSVGPLAAVGGLVLVAALGGVLFVRRESSAASPMVDLPLVLHRAVGPRLAAGTFSFLVLFGLLLTIPQNSDD
GAVAVGLLLATLPIAIGVAAPVAGTLVTRLGVRAVTSGAMVCVAGGLGLLAAGGSGPAVGVPSLALIGCGLGLFTPSNNG
AVMAAVPAQQSGMASGVVNMTRGLGTALGVSVSALAYAAGGFVATMLLLAAIALGGALLAATAAAPTVPGSALPRQREND
RAAP

Sequences:

>Translated_484_residues
MEIASRAGRMSGAAGALLREPARPARIRESPLATVLAVGTVCIGSFLGQLDASIVTIALADLRADFGMSLGLATWVSLSY
LVTLVATIAALGRLADMYGRKLLTLYGFALFTVASVGAALAPGIGVLIVCRVAQALGAAMLQANSVALIATTVEPRRLTA
ALGAQGAAQAFGLSLGPLAGGLLLELGGWRWVFWAHIPAGVIGFVLGVLLLPRTRAHAPRTPFDWRGLALLVPTVSAVLL
ALSVGESARSVGPLAAVGGLVLVAALGGVLFVRRESSAASPMVDLPLVLHRAVGPRLAAGTFSFLVLFGLLLTIPQNSDD
GAVAVGLLLATLPIAIGVAAPVAGTLVTRLGVRAVTSGAMVCVAGGLGLLAAGGSGPAVGVPSLALIGCGLGLFTPSNNG
AVMAAVPAQQSGMASGVVNMTRGLGTALGVSVSALAYAAGGFVATMLLLAAIALGGALLAATAAAPTVPGSALPRQREND
RAAP
>Mature_484_residues
MEIASRAGRMSGAAGALLREPARPARIRESPLATVLAVGTVCIGSFLGQLDASIVTIALADLRADFGMSLGLATWVSLSY
LVTLVATIAALGRLADMYGRKLLTLYGFALFTVASVGAALAPGIGVLIVCRVAQALGAAMLQANSVALIATTVEPRRLTA
ALGAQGAAQAFGLSLGPLAGGLLLELGGWRWVFWAHIPAGVIGFVLGVLLLPRTRAHAPRTPFDWRGLALLVPTVSAVLL
ALSVGESARSVGPLAAVGGLVLVAALGGVLFVRRESSAASPMVDLPLVLHRAVGPRLAAGTFSFLVLFGLLLTIPQNSDD
GAVAVGLLLATLPIAIGVAAPVAGTLVTRLGVRAVTSGAMVCVAGGLGLLAAGGSGPAVGVPSLALIGCGLGLFTPSNNG
AVMAAVPAQQSGMASGVVNMTRGLGTALGVSVSALAYAAGGFVATMLLLAAIALGGALLAATAAAPTVPGSALPRQREND
RAAP

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=27.7372262773723, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789042, Length=403, Percent_Identity=23.8213399503722, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788710, Length=326, Percent_Identity=25.4601226993865, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790195, Length=411, Percent_Identity=26.2773722627737, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788392, Length=227, Percent_Identity=30.8370044052863, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=391, Percent_Identity=24.8081841432225, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47998; Mature: 47998

Theoretical pI: Translated: 11.46; Mature: 11.46

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIASRAGRMSGAAGALLREPARPARIRESPLATVLAVGTVCIGSFLGQLDASIVTIALA
CCCHHHCCCCCCHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLRADFGMSLGLATWVSLSYLVTLVATIAALGRLADMYGRKLLTLYGFALFTVASVGAAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APGIGVLIVCRVAQALGAAMLQANSVALIATTVEPRRLTAALGAQGAAQAFGLSLGPLAG
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHCCCCCHHHC
GLLLELGGWRWVFWAHIPAGVIGFVLGVLLLPRTRAHAPRTPFDWRGLALLVPTVSAVLL
CHHEEECCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALSVGESARSVGPLAAVGGLVLVAALGGVLFVRRESSAASPMVDLPLVLHRAVGPRLAAG
HHHCCCHHHHCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHH
TFSFLVLFGLLLTIPQNSDDGAVAVGLLLATLPIAIGVAAPVAGTLVTRLGVRAVTSGAM
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VCVAGGLGLLAAGGSGPAVGVPSLALIGCGLGLFTPSNNGAVMAAVPAQQSGMASGVVNM
EEEECCCCEEEECCCCCCCCCCHHHHHHCCCCEECCCCCCEEEEECCCCCCCHHHHHHHH
TRGLGTALGVSVSALAYAAGGFVATMLLLAAIALGGALLAATAAAPTVPGSALPRQREND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
RAAP
CCCC
>Mature Secondary Structure
MEIASRAGRMSGAAGALLREPARPARIRESPLATVLAVGTVCIGSFLGQLDASIVTIALA
CCCHHHCCCCCCHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLRADFGMSLGLATWVSLSYLVTLVATIAALGRLADMYGRKLLTLYGFALFTVASVGAAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APGIGVLIVCRVAQALGAAMLQANSVALIATTVEPRRLTAALGAQGAAQAFGLSLGPLAG
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHCCCCCHHHC
GLLLELGGWRWVFWAHIPAGVIGFVLGVLLLPRTRAHAPRTPFDWRGLALLVPTVSAVLL
CHHEEECCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALSVGESARSVGPLAAVGGLVLVAALGGVLFVRRESSAASPMVDLPLVLHRAVGPRLAAG
HHHCCCHHHHCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHH
TFSFLVLFGLLLTIPQNSDDGAVAVGLLLATLPIAIGVAAPVAGTLVTRLGVRAVTSGAM
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VCVAGGLGLLAAGGSGPAVGVPSLALIGCGLGLFTPSNNGAVMAAVPAQQSGMASGVVNM
EEEECCCCEEEECCCCCCCCCCHHHHHHCCCCEECCCCCCEEEEECCCCCCCHHHHHHHH
TRGLGTALGVSVSALAYAAGGFVATMLLLAAIALGGALLAATAAAPTVPGSALPRQREND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
RAAP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]