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The map label for this gene is azoB [H]

Identifier: 158312343

GI number: 158312343

Start: 568079

End: 568966

Strand: Direct

Name: azoB [H]

Synonym: Franean1_0483

Alternate gene names: 158312343

Gene position: 568079-568966 (Clockwise)

Preceding gene: 158312342

Following gene: 158312344

Centisome position: 6.32

GC content: 72.18

Gene sequence:

>888_bases
GTGACCGTCCTCGTTGTCGGCGCTACCGGCCGAACCGCGTCGTACCTCGTCATGTGCCTGCTGCGGGACGGCGTGCCGGT
GCGGGCGCTGGTGCGGGACCGGGACAAGGCCGAGACCGTGTTCCGCGCCACCCGGCGTCCTGCCACCGGGCACCTCGAAA
TCGTCAGCGGTCCCTTCGACGACCCAGCGCTGCTCCTGTCTGCGACGAAGGGGGTCGACGCAGCCTTTCTCGCGCTGTCC
ACCAGCCCGCAGCAGATTGCGCTGGAGCGGAACGTCGTCGACGCCGCCGTCCGGGCGGAGCTGCCCCACTTGGTCCGGCT
GTCCGTGTTCGACGCGGACCTCGACGCCGGCTACGAGATCGGCCGACGCCACGGCGAACTCGACGCCTACCTGGCCGCGT
CCGGGGTGCCGCACACCCTGCTCCGCCCCACCTATTTCAGTAGCAACCTGCTGGTCGCCGCGCCCTCGATCGCGGCGACC
AGCCAGTGGGCCGGGCTGGCACCCGACGGCCGGGTCGCCCTCATCGACACCCGCGACGTCGCCGAGGCCGCCGCGGTCGT
GCTGCGGGACCCCGCTGGCCGCGGGGCGGCCTACCAGCTGACCGGGCCCGAGGCGCTGACATTCACCGAGATCGCCGAAT
GCTTCAGCCGGGTGCTGGGCCGGCGAATCAGCTACGACGCCGCCGACGAAGCCCATGTGCGCGCAGCCATGGCTGCCCGG
GGCGTACCGGGCTGGCTGGCCGACATCGCCGTCGGAATCGACCTGGCGATGCAGGCCGGCCGACACTCCCAGGTGACCAA
CGAGATTCGCGCGCTCACCGGCGCGGCGCCTCGCCCGTTGGAGGCGTTCATCGAATCCAACCGCGCCGCCTACACCCCGG
CGGGCTAA

Upstream 100 bases:

>100_bases
TCCCGGTCGTCATCCTCGACCGCACCCCGGCTCCGCTGCCGGCACCGGAGCCGGAGCCTACGCCGGTGCCGGCCTCGAGC
AAGGACGGGGACGACCGGCG

Downstream 100 bases:

>100_bases
CCGTGTGGGGCTCCAGAACCCCGATTTGGGCAGTGACCTGCATGTTTGTCTGCCGGACGGGGTCTGCGTCGGGCAGGCTG
GATGGTCATGGTGTGGTCGC

Product: NmrA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTVLVVGATGRTASYLVMCLLRDGVPVRALVRDRDKAETVFRATRRPATGHLEIVSGPFDDPALLLSATKGVDAAFLALS
TSPQQIALERNVVDAAVRAELPHLVRLSVFDADLDAGYEIGRRHGELDAYLAASGVPHTLLRPTYFSSNLLVAAPSIAAT
SQWAGLAPDGRVALIDTRDVAEAAAVVLRDPAGRGAAYQLTGPEALTFTEIAECFSRVLGRRISYDAADEAHVRAAMAAR
GVPGWLADIAVGIDLAMQAGRHSQVTNEIRALTGAAPRPLEAFIESNRAAYTPAG

Sequences:

>Translated_295_residues
MTVLVVGATGRTASYLVMCLLRDGVPVRALVRDRDKAETVFRATRRPATGHLEIVSGPFDDPALLLSATKGVDAAFLALS
TSPQQIALERNVVDAAVRAELPHLVRLSVFDADLDAGYEIGRRHGELDAYLAASGVPHTLLRPTYFSSNLLVAAPSIAAT
SQWAGLAPDGRVALIDTRDVAEAAAVVLRDPAGRGAAYQLTGPEALTFTEIAECFSRVLGRRISYDAADEAHVRAAMAAR
GVPGWLADIAVGIDLAMQAGRHSQVTNEIRALTGAAPRPLEAFIESNRAAYTPAG
>Mature_294_residues
TVLVVGATGRTASYLVMCLLRDGVPVRALVRDRDKAETVFRATRRPATGHLEIVSGPFDDPALLLSATKGVDAAFLALST
SPQQIALERNVVDAAVRAELPHLVRLSVFDADLDAGYEIGRRHGELDAYLAASGVPHTLLRPTYFSSNLLVAAPSIAATS
QWAGLAPDGRVALIDTRDVAEAAAVVLRDPAGRGAAYQLTGPEALTFTEIAECFSRVLGRRISYDAADEAHVRAAMAARG
VPGWLADIAVGIDLAMQAGRHSQVTNEIRALTGAAPRPLEAFIESNRAAYTPAG

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=262, Percent_Identity=30.9160305343511, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 31174; Mature: 31043

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLVVGATGRTASYLVMCLLRDGVPVRALVRDRDKAETVFRATRRPATGHLEIVSGPFD
CEEEEEECCCCHHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCC
DPALLLSATKGVDAAFLALSTSPQQIALERNVVDAAVRAELPHLVRLSVFDADLDAGYEI
CCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
GRRHGELDAYLAASGVPHTLLRPTYFSSNLLVAAPSIAATSQWAGLAPDGRVALIDTRDV
HHHCCCCCEEEECCCCCHHHCCCCEECCCEEEECCCCCCCCCCCCCCCCCCEEEEECHHH
AEAAAVVLRDPAGRGAAYQLTGPEALTFTEIAECFSRVLGRRISYDAADEAHVRAAMAAR
HHHHHHEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
GVPGWLADIAVGIDLAMQAGRHSQVTNEIRALTGAAPRPLEAFIESNRAAYTPAG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TVLVVGATGRTASYLVMCLLRDGVPVRALVRDRDKAETVFRATRRPATGHLEIVSGPFD
EEEEEECCCCHHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCC
DPALLLSATKGVDAAFLALSTSPQQIALERNVVDAAVRAELPHLVRLSVFDADLDAGYEI
CCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
GRRHGELDAYLAASGVPHTLLRPTYFSSNLLVAAPSIAATSQWAGLAPDGRVALIDTRDV
HHHCCCCCEEEECCCCCHHHCCCCEECCCEEEECCCCCCCCCCCCCCCCCCEEEEECHHH
AEAAAVVLRDPAGRGAAYQLTGPEALTFTEIAECFSRVLGRRISYDAADEAHVRAAMAAR
HHHHHHEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
GVPGWLADIAVGIDLAMQAGRHSQVTNEIRALTGAAPRPLEAFIESNRAAYTPAG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]