Click here to switch to the map view.

The map label for this gene is caiA [H]

Identifier: 157963848

GI number: 157963848

Start: 4902556

End: 4903692

Strand: Reverse

Name: caiA [H]

Synonym: Spea_4037

Alternate gene names: 157963848

Gene position: 4903692-4902556 (Counterclockwise)

Preceding gene: 157963849

Following gene: 157963847

Centisome position: 94.77

GC content: 48.11

Gene sequence:

>1137_bases
ATGGATTTTAAATTAACCGACGAACAAGAGCTTTTTGTTGCAGGTATTCGTGACCTGATGGCTAAAGAAAACTGGGAAAG
TTATTTCGCAGAGTGCGATGAGAACAGCCAGTACCCAGAGCGTTTTGTTAAAGAGCTTGCTGAAATGGGTATCGACAGCT
TGTTGTTGCCAGAAGAGCACGGCGGATTCAACGAAGGCATGGTAACGCTAGCGGTGATCTGGGAAGAGTTAGGCCGCTTA
GGTGCGCCAACTTACGTTCTATACCAACTACCTGGTATCACTACCATGCTAAGACACGGTAGCCCAGAGCAGATCGATAA
GATCATGACACTACGTGGCACAGGTAAGCAGATGTGGAACTCAGCGATCACTGAGCCAAGTGCAGGTTCTGACGTGGGTA
GCTTGTTAACTACTTATAAGCGTCGTGATGGCAAGGTGTATTTGACCGGTAGTAAGTGCTTCATTACCAGTGCGGCGGGT
GCGCCTTACATCGTGGTTATGGCTAAAGATGCAGACTCAGAGAAGCCAATCTTCACCGAGTGGTTTGTCGATATGTCTAA
GCCAGGTATCAAGATGAGCAAGCTACCAAAGCTAGGTTTGAAGATGGACAGCTGCTGTGAGCTTCAATTTGACGAGTTTG
AGCTAGAAGAGTCAGACATGTTCGGTATCGAAGGTAATGGCTTCATGCGTGTTAAAGAGGAGTTTGACTCTGAGCGCTTC
CTTGTGGCCCTAACTAACTACGGTGCGGCTTACTGTGCATTCGAAGATGCGGCCAAGTACGCTAACCAACGTGTTCAGTT
CGGTGAGGCTATTGGCCGTTACCAGTTGATCCAAGAGAAGTTTGCCCATATGGCTATCAAGCTAAACAGCATGAAGAACA
TGGTCTATGAAGCAGCGTGGAAGTGTGACAACGGCACTATGACATCGGGCGACTCGGCAATGTGTAAGTTCTACTGCGCC
AATGCAGGTTTTGAAGTCATCGATTCCGCTATGCAAGTGCTAGGTGGTTACGCGATTGCAGGCGAGCACCGTATCAGCCG
TATGTGGCGTGATATTCGTGTTGACCGTGTCTCTGGTGGTTCTGATGAAATGCAGATCTTAACTTTAGGCCGTGAAGTGT
TAAAGCGCTATCGCTAA

Upstream 100 bases:

>100_bases
TCTCTTTCTTGAAAGATGCTCGTAAAAATAATTGGAAACCTGTCGGTGAAGAGGCTTAGCCACACCTGGGATCCACAGTT
GAAATTTAGAGGAATAAATA

Downstream 100 bases:

>100_bases
TCAACCGTGGTGATAAACAGACTAGGTACTGGGTGAGTAAGTACTTAGTCTGAACGGTGTTTAGTGTGGGATTTGAGAGC
CTTTTAATATTAAGCAGATC

Product: crotonobetainyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA reductase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEHGGFNEGMVTLAVIWEELGRL
GAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWNSAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAG
APYIVVMAKDADSEKPIFTEWFVDMSKPGIKMSKLPKLGLKMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAWKCDNGTMTSGDSAMCKFYCA
NAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGGSDEMQILTLGREVLKRYR

Sequences:

>Translated_378_residues
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEHGGFNEGMVTLAVIWEELGRL
GAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWNSAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAG
APYIVVMAKDADSEKPIFTEWFVDMSKPGIKMSKLPKLGLKMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAWKCDNGTMTSGDSAMCKFYCA
NAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGGSDEMQILTLGREVLKRYR
>Mature_378_residues
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEHGGFNEGMVTLAVIWEELGRL
GAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWNSAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAG
APYIVVMAKDADSEKPIFTEWFVDMSKPGIKMSKLPKLGLKMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAWKCDNGTMTSGDSAMCKFYCA
NAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGGSDEMQILTLGREVLKRYR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma- butyrobetainyl-CoA [H]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=382, Percent_Identity=29.0575916230367, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI226958412, Length=368, Percent_Identity=27.7173913043478, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI226958414, Length=339, Percent_Identity=28.023598820059, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4557231, Length=383, Percent_Identity=26.8929503916449, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI187960098, Length=383, Percent_Identity=26.8929503916449, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4501857, Length=376, Percent_Identity=25.531914893617, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4501859, Length=378, Percent_Identity=25.1322751322751, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI21361497, Length=357, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI7656849, Length=375, Percent_Identity=25.6, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI76496475, Length=332, Percent_Identity=28.9156626506024, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4557235, Length=332, Percent_Identity=28.9156626506024, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI38505218, Length=343, Percent_Identity=23.6151603498542, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4503943, Length=376, Percent_Identity=22.0744680851064, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786223, Length=380, Percent_Identity=80.2631578947368, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI87081958, Length=382, Percent_Identity=47.3821989528796, Blast_Score=354, Evalue=6e-99,
Organism=Escherichia coli, GI87082384, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538396, Length=382, Percent_Identity=31.413612565445, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17569725, Length=357, Percent_Identity=27.4509803921569, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17570075, Length=383, Percent_Identity=26.3707571801567, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17534899, Length=385, Percent_Identity=25.974025974026, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17533517, Length=362, Percent_Identity=27.3480662983425, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17508101, Length=381, Percent_Identity=24.6719160104987, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17506239, Length=380, Percent_Identity=25.2631578947368, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71985184, Length=377, Percent_Identity=27.8514588859416, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17551932, Length=365, Percent_Identity=24.3835616438356, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17505929, Length=365, Percent_Identity=24.9315068493151, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71985192, Length=317, Percent_Identity=28.0757097791798, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990804, Length=294, Percent_Identity=24.8299319727891, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32563615, Length=230, Percent_Identity=28.695652173913, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534353, Length=284, Percent_Identity=24.6478873239437, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355753, Length=344, Percent_Identity=29.6511627906977, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21356377, Length=378, Percent_Identity=27.7777777777778, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24646207, Length=347, Percent_Identity=27.3775216138329, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI281363737, Length=351, Percent_Identity=29.9145299145299, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24666513, Length=380, Percent_Identity=26.5789473684211, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24660351, Length=378, Percent_Identity=25.9259259259259, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19920834, Length=279, Percent_Identity=26.1648745519713, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42588; Mature: 42588

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00072 ACYL_COA_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEH
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGFNEGMVTLAVIWEELGRLGAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWN
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHH
SAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAGAPYIVVMAKDADSEKPIFTE
HHCCCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHH
WFVDMSKPGIKMSKLPKLGLKMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
HHHHCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEHHHCCCCEE
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAW
EEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KCDNGTMTSGDSAMCKFYCANAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGG
ECCCCCCCCCCHHEEEEEECCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHEECCCC
SDEMQILTLGREVLKRYR
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEH
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGFNEGMVTLAVIWEELGRLGAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWN
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHH
SAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAGAPYIVVMAKDADSEKPIFTE
HHCCCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHH
WFVDMSKPGIKMSKLPKLGLKMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
HHHHCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEHHHCCCCEE
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAW
EEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KCDNGTMTSGDSAMCKFYCANAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGG
ECCCCCCCCCCHHEEEEEECCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHEECCCC
SDEMQILTLGREVLKRYR
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA