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The map label for this gene is silB [H]

Identifier: 157963799

GI number: 157963799

Start: 4842755

End: 4844677

Strand: Direct

Name: silB [H]

Synonym: Spea_3988

Alternate gene names: 157963799

Gene position: 4842755-4844677 (Clockwise)

Preceding gene: 157963798

Following gene: 157963800

Centisome position: 93.59

GC content: 50.65

Gene sequence:

>1923_bases
ATGAGTTTTTTTAAGCCAAACAAAGCGGCGACGCCGCTGATCTGCGCATTCAGTTTTATGCTTGTGGCTACGCCACAACA
GGCATATAGCCAAACTGCAGAAACAACATATCAACACGCTCAGGCCCACCAGCATGAGTCAACTTACTACTGCCCCATGC
ATCCGGAAGTGACCAGCCATGAGCCCGGTCGCTGCCCTGAGTGCAAGATGTTCTTAGTTAAGGACGAAGAGCAGGACAGT
TCGACTCACGACAATCATGCAACTGTAACTAAGAGTCAAGATTACTACTGCCCCATGCATCCGGAAGTGACCAGCCACGA
GCCCGGTCGCTGCCCTGAGTGCAAGATGTTCTTAGTTAAGGATGAAGAACAGGACAGTTCAAATCACGACAATCATGCGG
CTGTAAGCCAGAATTACTACTGCCCGATGCATCCAGAAGTGACCAGCCATGAGCCCGGTCGCTGCCCTGAGTGCAAGATG
TTCTTAGTTAAGGATGAAGAACAGGACAGTTCAAATCACGACAATCATGCAGCTGTCACTAATGAGTACTACTGCCCGAT
GCATCCGGAAGTGACCAGCCATGAGCCCGGTCGCTGCCCTGAGTGCAAGATGTTCTTGGTGAAAGAGGAGGAAGAGGAGT
CTGTGCCTGATGAGCACGCTGGCCATAACCACACGACTCAATCGACCTCCTCAACTCAGCAGTCTCAAGCGGATAAGATC
CTAAGCCAACCTAAGCCGACACTCTCGCCTGCAGCCCAGAGCAGCGGTGATGGCAACGTAAAATATGTTTGCCCCATGCA
TCCTCACGTCGTCTCCGATGTTCCTGGCTCCTGCCCGATATGCGGCATGGACCTAGAGAAAGTCACCATAGGCGGCGTAG
GTGAAGAAGTGGTCGTTGGTGTATCTGGCGGTATGCAGCAAGCATTAGGCATGCGCAGCGAGCAAATCACCGAAGGCACC
CTTTGGAAGCTGGTAAAAACCATAGGCACGGTGGAGTATAACGAAAACGCCATCGGCCATAGCCATACCCGCGTTAACGG
CTGGATTGAAACCTTAATGGTGCATAACGTCGGCCAGCGGGTGAAGAAAGGCCAATTACTTTATGAGCTTTACTCTCCAG
AGCTGATTAATGCCCAAGACGATTATATGCAAGCGGTGGACTATCTAACCCAAGACAAAAGCCGCGGCCAAGGCTTACTA
CGCAAGGCACGCTTACGTCTTGAGTTACTGGGTGTGAGCTCCGACACTATCAAGCAATTAGAACGCACAGGTAAAACCAT
CTACCGTGTGCCCTTTTATGCCGAGCAAGATGGCTTTATCAGCAAGCTAACCGTACGTCATGGCATGTATGTGCAGCCCG
GCGATACCTTGTTTGAAATCGTCGATTTAAGCAGTGTATGGGTGATTGCCGACGTATTTGAAAATGAACAGAGCTGGCTC
GAACAGGGTCGTCCTGTAGAAGTCACCTCTGCTGCTCAAGGGCTATTCGATCTCGAGTCGACTATCGACTATATCTACCC
TGAGCTTGACCCTGTATCCCGTGCAATGCGCGTGCGGATTAAACTAGACAATCCCGATAAACTGCTCAAACCCGGTACCT
TGGTCGATGTAAAACTCTTCGGTGGCCCTAAACGTGAAGTTCTGGCGATCCCAACTGAAGCGCTTATTCTTACTGGCCGC
GAAAACCGAGTCGTAGTGCAGCGTGATGATAACCGCTTTGCTTCTGTGCCCGTAAAAGTGGGCATGATTGCCCAAGGCAA
GGCTGAGATTATCGAAGGCCTAAAGGTTGGCGACAAGGTAATTGTTTCTGGTCAGTTCTTATTAGACTCGGAAGCCAGTA
TCCAGGGTAGCTTACAGCGCTTAAGCGGTAGCAATAGTAGCGAAAACAATAACGCTGCTAGCGATCCACACGCAAACCAT
TAA

Upstream 100 bases:

>100_bases
CCCTAAGTGTGGGATGGATCTAGAGCCTATCGCGACAAGCGACAAGGCCACTAAATCTACTCACATGCATCACTAAGACC
TAGGGTGGAGATAAGGCATT

Downstream 100 bases:

>100_bases
TCGGAGACTGACTATGTTAGAAAAAATTATCAGTGCCTCGATTAAACAGCGGGCGATGGTGTTAGTGCTTACTGCGGTGA
TCGCCTTAATCGGCTATCAA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MSFFKPNKAATPLICAFSFMLVATPQQAYSQTAETTYQHAQAHQHESTYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDS
STHDNHATVTKSQDYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDSSNHDNHAAVSQNYYCPMHPEVTSHEPGRCPECKM
FLVKDEEQDSSNHDNHAAVTNEYYCPMHPEVTSHEPGRCPECKMFLVKEEEEESVPDEHAGHNHTTQSTSSTQQSQADKI
LSQPKPTLSPAAQSSGDGNVKYVCPMHPHVVSDVPGSCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEQITEGT
LWKLVKTIGTVEYNENAIGHSHTRVNGWIETLMVHNVGQRVKKGQLLYELYSPELINAQDDYMQAVDYLTQDKSRGQGLL
RKARLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWL
EQGRPVEVTSAAQGLFDLESTIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKREVLAIPTEALILTGR
ENRVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLKVGDKVIVSGQFLLDSEASIQGSLQRLSGSNSSENNNAASDPHANH

Sequences:

>Translated_640_residues
MSFFKPNKAATPLICAFSFMLVATPQQAYSQTAETTYQHAQAHQHESTYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDS
STHDNHATVTKSQDYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDSSNHDNHAAVSQNYYCPMHPEVTSHEPGRCPECKM
FLVKDEEQDSSNHDNHAAVTNEYYCPMHPEVTSHEPGRCPECKMFLVKEEEEESVPDEHAGHNHTTQSTSSTQQSQADKI
LSQPKPTLSPAAQSSGDGNVKYVCPMHPHVVSDVPGSCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEQITEGT
LWKLVKTIGTVEYNENAIGHSHTRVNGWIETLMVHNVGQRVKKGQLLYELYSPELINAQDDYMQAVDYLTQDKSRGQGLL
RKARLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWL
EQGRPVEVTSAAQGLFDLESTIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKREVLAIPTEALILTGR
ENRVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLKVGDKVIVSGQFLLDSEASIQGSLQRLSGSNSSENNNAASDPHANH
>Mature_639_residues
SFFKPNKAATPLICAFSFMLVATPQQAYSQTAETTYQHAQAHQHESTYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDSS
THDNHATVTKSQDYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDSSNHDNHAAVSQNYYCPMHPEVTSHEPGRCPECKMF
LVKDEEQDSSNHDNHAAVTNEYYCPMHPEVTSHEPGRCPECKMFLVKEEEEESVPDEHAGHNHTTQSTSSTQQSQADKIL
SQPKPTLSPAAQSSGDGNVKYVCPMHPHVVSDVPGSCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEQITEGTL
WKLVKTIGTVEYNENAIGHSHTRVNGWIETLMVHNVGQRVKKGQLLYELYSPELINAQDDYMQAVDYLTQDKSRGQGLLR
KARLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWLE
QGRPVEVTSAAQGLFDLESTIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKREVLAIPTEALILTGRE
NRVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLKVGDKVIVSGQFLLDSEASIQGSLQRLSGSNSSENNNAASDPHANH

Specific function: Component of the sil cation efflux system that confers resistance to silver. May be part of a three-component cation/proton antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=369, Percent_Identity=30.0813008130081, Blast_Score=149, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 71171; Mature: 71039

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFKPNKAATPLICAFSFMLVATPQQAYSQTAETTYQHAQAHQHESTYYCPMHPEVTSH
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
EPGRCPECKMFLVKDEEQDSSTHDNHATVTKSQDYYCPMHPEVTSHEPGRCPECKMFLVK
CCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCEEEEEE
DEEQDSSNHDNHAAVSQNYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDSSNHDNHAAVT
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEC
NEYYCPMHPEVTSHEPGRCPECKMFLVKEEEEESVPDEHAGHNHTTQSTSSTQQSQADKI
CCEECCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCHHCCCCCCCCCCCHHHHHHHHHH
LSQPKPTLSPAAQSSGDGNVKYVCPMHPHVVSDVPGSCPICGMDLEKVTIGGVGEEVVVG
HHCCCCCCCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCEECCCCCEEEECCCCCEEEEE
VSGGMQQALGMRSEQITEGTLWKLVKTIGTVEYNENAIGHSHTRVNGWIETLMVHNVGQR
CCCCHHHHHCCCHHHCCHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VKKGQLLYELYSPELINAQDDYMQAVDYLTQDKSRGQGLLRKARLRLELLGVSSDTIKQL
HHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEECCCHHHHHHH
ERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWL
HHCCCEEEEECEEECCCCCEEEEECCCCCEECCCCCEEHHHCCCCEEEEEHHHHCHHHHH
EQGRPVEVTSAAQGLFDLESTIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLF
HCCCCEEEEHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHCCCCCEEEEEEC
GGPKREVLAIPTEALILTGRENRVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLKVGDKV
CCCCCCEEECCCEEEEEECCCCEEEEEECCCCEEECCEEEEEEECCHHHHHHHHCCCCEE
IVSGQFLLDSEASIQGSLQRLSGSNSSENNNAASDPHANH
EEECEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFFKPNKAATPLICAFSFMLVATPQQAYSQTAETTYQHAQAHQHESTYYCPMHPEVTSH
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
EPGRCPECKMFLVKDEEQDSSTHDNHATVTKSQDYYCPMHPEVTSHEPGRCPECKMFLVK
CCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCEEEEEE
DEEQDSSNHDNHAAVSQNYYCPMHPEVTSHEPGRCPECKMFLVKDEEQDSSNHDNHAAVT
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEC
NEYYCPMHPEVTSHEPGRCPECKMFLVKEEEEESVPDEHAGHNHTTQSTSSTQQSQADKI
CCEECCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCHHCCCCCCCCCCCHHHHHHHHHH
LSQPKPTLSPAAQSSGDGNVKYVCPMHPHVVSDVPGSCPICGMDLEKVTIGGVGEEVVVG
HHCCCCCCCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCEECCCCCEEEECCCCCEEEEE
VSGGMQQALGMRSEQITEGTLWKLVKTIGTVEYNENAIGHSHTRVNGWIETLMVHNVGQR
CCCCHHHHHCCCHHHCCHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VKKGQLLYELYSPELINAQDDYMQAVDYLTQDKSRGQGLLRKARLRLELLGVSSDTIKQL
HHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEECCCHHHHHHH
ERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWL
HHCCCEEEEECEEECCCCCEEEEECCCCCEECCCCCEEHHHCCCCEEEEEHHHHCHHHHH
EQGRPVEVTSAAQGLFDLESTIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLF
HCCCCEEEEHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHCCCCCEEEEEEC
GGPKREVLAIPTEALILTGRENRVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLKVGDKV
CCCCCCEEECCCEEEEEECCCCEEEEEECCCCEEECCEEEEEEECCHHHHHHHHCCCCEE
IVSGQFLLDSEASIQGSLQRLSGSNSSENNNAASDPHANH
EEECEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9930866 [H]