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The map label for this gene is rpoH [H]

Identifier: 157963788

GI number: 157963788

Start: 4832882

End: 4833739

Strand: Reverse

Name: rpoH [H]

Synonym: Spea_3977

Alternate gene names: 157963788

Gene position: 4833739-4832882 (Counterclockwise)

Preceding gene: 157963789

Following gene: 157963786

Centisome position: 93.41

GC content: 44.99

Gene sequence:

>858_bases
ATGACAGTTCAAACGCAATCAATGGCACTAATGGTTCCCCAAAGTAGTGGAAGCCTCGAGTCTTATGTTCATTCAGCACA
CAACATCCCAATGTTGGAAGCGGATAAGGAATATGAGCTAGCGAAGCGTCTGCAAGAAACTGGTGACCTACAGGCTGCGA
AACAGTTGATTATGTCGCACTTACGTTTTGTGGTACATGTTGCAAAAGGTTACTCTGGCTATGGTCTTCCACAAGCTGAC
TTGATCCAAGAAGGTAATATCGGCCTGATGAAGGCGGTTAAGCGTTTCGATCCAGATGTGGGCGTTCGCTTAGTATCGTT
TGCCGTGCACTGGATTAAAGCTGAAATTCACGAATATGTGCTTAAAAACTGGCGCATAGTTAAAGTGGCGACCACTAAAG
CACAACGTAAGTTGTTCTTTAACCTGCGTAAGGCTAAAAAGCGTCTAGGCTGGTTTAGCGATAGTGAAGTGGGCATGGTA
GCTGAGAGTCTCGGTGTGTCGAAAGCCGATGTGACCGAGATGGAGTCACGCATGGCGGCGCAAGATCCTGCATTTGATCT
GGCTAGCGACAACGATGATGAACAAGATTATGCTCCTATGCATTACCTTGAAGATCATTCATCAGATTTAGCGTCTCAAG
TTGAAAATGACAACTGGGAACAGAATAACCAAGCGCGTTTGTTATCGGCTATTAAGACTCTCGATGAGCGTAGCCAACAT
ATTCTAAAAGCGCGTTGGTTAGATGAAGACAAGACCACATTGCAAGATTTAGCGGCGACTTATCAAGTGTCGGCTGAACG
TATTAGGCAATTGGAAAAGAATGCAATGAATAAGCTTAAAGGCTGTATGGAAGCCTAG

Upstream 100 bases:

>100_bases
ATATTTTGTCAATTAGTCGATAGTTCATTGTCCATTTCAGCAAGGAATGGCCAGAAAGTTGATTTTAGCAAGACATTTGT
AAGAAGTAGTAGGAGCGTAA

Downstream 100 bases:

>100_bases
TTTCCATTGTGTGCTCAATGGCTTTAGCAATAAATGAAACCTGCTTACCCAAGCAGGTTTTTTTATGTCTGGAACATTTA
TGCCAAAAGCCCATTTAAGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTVQTQSMALMVPQSSGSLESYVHSAHNIPMLEADKEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMV
AESLGVSKADVTEMESRMAAQDPAFDLASDNDDEQDYAPMHYLEDHSSDLASQVENDNWEQNNQARLLSAIKTLDERSQH
ILKARWLDEDKTTLQDLAATYQVSAERIRQLEKNAMNKLKGCMEA

Sequences:

>Translated_285_residues
MTVQTQSMALMVPQSSGSLESYVHSAHNIPMLEADKEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMV
AESLGVSKADVTEMESRMAAQDPAFDLASDNDDEQDYAPMHYLEDHSSDLASQVENDNWEQNNQARLLSAIKTLDERSQH
ILKARWLDEDKTTLQDLAATYQVSAERIRQLEKNAMNKLKGCMEA
>Mature_284_residues
TVQTQSMALMVPQSSGSLESYVHSAHNIPMLEADKEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQADL
IQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMVA
ESLGVSKADVTEMESRMAAQDPAFDLASDNDDEQDYAPMHYLEDHSSDLASQVENDNWEQNNQARLLSAIKTLDERSQHI
LKARWLDEDKTTLQDLAATYQVSAERIRQLEKNAMNKLKGCMEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=66.8989547038328, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1789098, Length=268, Percent_Identity=26.865671641791, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32285; Mature: 32154

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQTQSMALMVPQSSGSLESYVHSAHNIPMLEADKEYELAKRLQETGDLQAAKQLIMSH
CCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LRFVVHVAKGYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMVAESLGVSKADVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
QDPAFDLASDNDDEQDYAPMHYLEDHSSDLASQVENDNWEQNNQARLLSAIKTLDERSQH
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ILKARWLDEDKTTLQDLAATYQVSAERIRQLEKNAMNKLKGCMEA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVQTQSMALMVPQSSGSLESYVHSAHNIPMLEADKEYELAKRLQETGDLQAAKQLIMSH
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LRFVVHVAKGYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNLRKAKKRLGWFSDSEVGMVAESLGVSKADVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
QDPAFDLASDNDDEQDYAPMHYLEDHSSDLASQVENDNWEQNNQARLLSAIKTLDERSQH
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ILKARWLDEDKTTLQDLAATYQVSAERIRQLEKNAMNKLKGCMEA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]