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The map label for this gene is ykoW [H]

Identifier: 157963764

GI number: 157963764

Start: 4806122

End: 4808266

Strand: Direct

Name: ykoW [H]

Synonym: Spea_3953

Alternate gene names: 157963764

Gene position: 4806122-4808266 (Clockwise)

Preceding gene: 157963760

Following gene: 157963766

Centisome position: 92.88

GC content: 45.73

Gene sequence:

>2145_bases
ATGAGTTTGTTAACCTGGTCTGAATTGTACCCCCGTGTATCCTCTACAGATCCGGCTCATTCCGAGCAATTCTTGCTCTG
GCAAAATGAATCTGAATCAACCGCTCTGGCCATATTACAGGGGCACCACTTTCTTGCTGCCACCCCCGCAGCGCTCAATT
ATTTTGAAAGCCAAGAGCAGAACTTTATTCATGCCACCCCCTTCGACTTCTCTCCCAGACTGCAGTCTTCAGGAAGAAAT
AGTGTCGAGTATGGCCAGCAAATGATAAAAAAGGCGGCCAAGGGCGAGGTCGTCTCATTGAGTTGGTTGCATATGAGTCA
GCAAGGTAAAGAGCTGCCTACCGTTTTAACCCTTTACCCGGCTCAACTCGATAACATTAAGGTGATGATAGTCTCCTTCA
AGCCTTTAGACAGGCGAAAGCGCAGTAGAGAGAGTATCAGTAATGGCTTTGAAACCTTGCCTAAAGAGCTGATGTCGATA
ACCCTAGAAGACAGCGCCGAAGCCGTTTATATCACAGATGAAGATGATAGAATTTTGGCGGTCAATAAGGCCATGTGCCG
GATCTGTGGCTATAGTGCCGAACATTTGCTCAGTAAGACTCCGAGCGATATCAAGCTAGAATTACAACACCAAAATCAAG
CACCCGATAACCCTCTTAAAGGCAAGGGAAGCTGGCAAGGGGAAGTTTGGAAGTGTCGCTCAGATGGTTCAACATTCCCG
GCATGGCAAAGTTGCCGACGGATCTACGCCGATAACACAGTATTTTACGTTAACTTGTTTAGCGATATTAGTGAGAAGAA
GGCGCTCGAAAACAAGCTGACTCAACAAGCCATGTATGACAAGCTAACTGGGTTGCCAAATCGTTTCCATCTGATCCAAT
TGCTCAATAAGGCGATCAACAACATCAAGCAATCTCCCGACTCGATCGGCGCGCTGATGTTTTTAGATCTCAATGGTTTT
AAGAATATTAACGACAGCTTTGGTCACTCGACCGGAGATAAAGTCTTACAACTCGTTGCAGCTCGTCTCGAAGCTTGTTG
TATCGAGAATGCTGAAATCGCCCGCCTCGGTGGTGATGAGTTCACCTTAGTCTTACCTCAGTGCCACAGCAGGCAAGAAA
TTGAAGCATTTTCTGAGCTGATATTGTCATTGTTTGAGGCCCCCTTCGAGATTAATGGTCAGAAATTCTATCTTGGCACC
AGTATTGGGATCTCGCTATTTCCGCAGCAAAGTGACGAGCCCAATCAGCTCTTAAGCATGGCCGACACCGCTATGTATTC
GGCTAAAAATAGCCCTAAGCATATCCGTTTCTATAACGCGACCATTCGCGAAGCGGCCGAGAAAAAACTACATACTCTAA
ATGACCTACGCCACGCTCAAAGTCTTGGCCAATTCAGTTTAGTTTATCAAAGCATTGTCGATTTAGAATCCAATAGAACA
GTGGCAATTGAAGCTCTATTGCGCTGGAATCAGAGTAATGGTGAGGTTATTGAAGCCCATGATTTTATTCCTCTACTAGA
GGAAGCTGGCCTGATGGTCAACGTTGGCCAATGGGCGCTAGAACAAGCTTGCATTCAAATGCTGAAATGGCAACAAGACT
ACGACTCAGAACTTAAAATCAGCGTGAATATCTCAAGATCTCAGCTAGAACACCCAGACTTTATCTCCATGCTTGAAGGG
TTACTGACGCGGACTTGCCTGCCTGCTAGCGCATTGATTATTGAGATCACCGAGAATGCTTTTATACACAGGCCTAAATT
GATGACAAAAGTCTTAGCTCAGTTAAAAGAGATGGGTATTTGCATCACGATTGACGACTTTGGCGCTGGATTGTCCTCAT
TAAGTCGGCTCAGCAGCTTGCCTATCGACAGCCTGAAAATTGATGCAGGCTTTGCCCAACGCTTAGATTGCAATCAAGGT
CGAAAGCTTTGCAAAGCCATTATGCAACTAGCGCAAACCTTAGAGATCAATTTTGTTGCTGAAGGGGTAGAAACTCAAGC
CCAAAGAGATATGCTGTTGAGTCTAGGAAAAGGCATGGGCCAAGGGTATTTCTATAATAGGCCAACAGCGGCTGAAAACT
TTACCCATGAGGTACTCGACCCAATCTACGCGACAACGCAGACTAAGAATCTAGCGGCAAGATGA

Upstream 100 bases:

>100_bases
TTTTTGACGCCAAATCGACTAAGCTATATCAAAATATAATTTTATTGTCGGAATAATCAGTAATTTGCCTCTAAAATGTC
ATGCCAAAAGGGAAGAGTGT

Downstream 100 bases:

>100_bases
AAGGTAGCTACGCCAGCACTCTGAATAAGTTATTCAGTGTCGCCCCCTGCAAAAGCTGTTGCCGACGTAGCCCCTCATTA
TCACCATAAGATGATGATGT

Product: PAS/PAC sensor-containing diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSLLTWSELYPRVSSTDPAHSEQFLLWQNESESTALAILQGHHFLAATPAALNYFESQEQNFIHATPFDFSPRLQSSGRN
SVEYGQQMIKKAAKGEVVSLSWLHMSQQGKELPTVLTLYPAQLDNIKVMIVSFKPLDRRKRSRESISNGFETLPKELMSI
TLEDSAEAVYITDEDDRILAVNKAMCRICGYSAEHLLSKTPSDIKLELQHQNQAPDNPLKGKGSWQGEVWKCRSDGSTFP
AWQSCRRIYADNTVFYVNLFSDISEKKALENKLTQQAMYDKLTGLPNRFHLIQLLNKAINNIKQSPDSIGALMFLDLNGF
KNINDSFGHSTGDKVLQLVAARLEACCIENAEIARLGGDEFTLVLPQCHSRQEIEAFSELILSLFEAPFEINGQKFYLGT
SIGISLFPQQSDEPNQLLSMADTAMYSAKNSPKHIRFYNATIREAAEKKLHTLNDLRHAQSLGQFSLVYQSIVDLESNRT
VAIEALLRWNQSNGEVIEAHDFIPLLEEAGLMVNVGQWALEQACIQMLKWQQDYDSELKISVNISRSQLEHPDFISMLEG
LLTRTCLPASALIIEITENAFIHRPKLMTKVLAQLKEMGICITIDDFGAGLSSLSRLSSLPIDSLKIDAGFAQRLDCNQG
RKLCKAIMQLAQTLEINFVAEGVETQAQRDMLLSLGKGMGQGYFYNRPTAAENFTHEVLDPIYATTQTKNLAAR

Sequences:

>Translated_714_residues
MSLLTWSELYPRVSSTDPAHSEQFLLWQNESESTALAILQGHHFLAATPAALNYFESQEQNFIHATPFDFSPRLQSSGRN
SVEYGQQMIKKAAKGEVVSLSWLHMSQQGKELPTVLTLYPAQLDNIKVMIVSFKPLDRRKRSRESISNGFETLPKELMSI
TLEDSAEAVYITDEDDRILAVNKAMCRICGYSAEHLLSKTPSDIKLELQHQNQAPDNPLKGKGSWQGEVWKCRSDGSTFP
AWQSCRRIYADNTVFYVNLFSDISEKKALENKLTQQAMYDKLTGLPNRFHLIQLLNKAINNIKQSPDSIGALMFLDLNGF
KNINDSFGHSTGDKVLQLVAARLEACCIENAEIARLGGDEFTLVLPQCHSRQEIEAFSELILSLFEAPFEINGQKFYLGT
SIGISLFPQQSDEPNQLLSMADTAMYSAKNSPKHIRFYNATIREAAEKKLHTLNDLRHAQSLGQFSLVYQSIVDLESNRT
VAIEALLRWNQSNGEVIEAHDFIPLLEEAGLMVNVGQWALEQACIQMLKWQQDYDSELKISVNISRSQLEHPDFISMLEG
LLTRTCLPASALIIEITENAFIHRPKLMTKVLAQLKEMGICITIDDFGAGLSSLSRLSSLPIDSLKIDAGFAQRLDCNQG
RKLCKAIMQLAQTLEINFVAEGVETQAQRDMLLSLGKGMGQGYFYNRPTAAENFTHEVLDPIYATTQTKNLAAR
>Mature_713_residues
SLLTWSELYPRVSSTDPAHSEQFLLWQNESESTALAILQGHHFLAATPAALNYFESQEQNFIHATPFDFSPRLQSSGRNS
VEYGQQMIKKAAKGEVVSLSWLHMSQQGKELPTVLTLYPAQLDNIKVMIVSFKPLDRRKRSRESISNGFETLPKELMSIT
LEDSAEAVYITDEDDRILAVNKAMCRICGYSAEHLLSKTPSDIKLELQHQNQAPDNPLKGKGSWQGEVWKCRSDGSTFPA
WQSCRRIYADNTVFYVNLFSDISEKKALENKLTQQAMYDKLTGLPNRFHLIQLLNKAINNIKQSPDSIGALMFLDLNGFK
NINDSFGHSTGDKVLQLVAARLEACCIENAEIARLGGDEFTLVLPQCHSRQEIEAFSELILSLFEAPFEINGQKFYLGTS
IGISLFPQQSDEPNQLLSMADTAMYSAKNSPKHIRFYNATIREAAEKKLHTLNDLRHAQSLGQFSLVYQSIVDLESNRTV
AIEALLRWNQSNGEVIEAHDFIPLLEEAGLMVNVGQWALEQACIQMLKWQQDYDSELKISVNISRSQLEHPDFISMLEGL
LTRTCLPASALIIEITENAFIHRPKLMTKVLAQLKEMGICITIDDFGAGLSSLSRLSSLPIDSLKIDAGFAQRLDCNQGR
KLCKAIMQLAQTLEINFVAEGVETQAQRDMLLSLGKGMGQGYFYNRPTAAENFTHEVLDPIYATTQTKNLAAR

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=445, Percent_Identity=32.1348314606742, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI226510982, Length=430, Percent_Identity=30.9302325581395, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI87081921, Length=450, Percent_Identity=30.4444444444444, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787055, Length=444, Percent_Identity=26.1261261261261, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1790496, Length=240, Percent_Identity=29.5833333333333, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081845, Length=248, Percent_Identity=32.258064516129, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081743, Length=226, Percent_Identity=31.858407079646, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1788849, Length=455, Percent_Identity=25.2747252747253, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788381, Length=543, Percent_Identity=24.3093922651934, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1788502, Length=241, Percent_Identity=31.5352697095436, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786507, Length=235, Percent_Identity=26.3829787234043, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI87081980, Length=235, Percent_Identity=31.063829787234, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788956, Length=160, Percent_Identity=36.875, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87082096, Length=434, Percent_Identity=26.036866359447, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI87081881, Length=203, Percent_Identity=30.5418719211823, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87081977, Length=191, Percent_Identity=30.8900523560209, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1787802, Length=220, Percent_Identity=27.7272727272727, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1786584, Length=188, Percent_Identity=28.1914893617021, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=32.484076433121, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI87082007, Length=177, Percent_Identity=29.9435028248588, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1787410, Length=229, Percent_Identity=26.6375545851528, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1787262, Length=187, Percent_Identity=25.668449197861, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 80120; Mature: 79989

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50112 PAS ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLTWSELYPRVSSTDPAHSEQFLLWQNESESTALAILQGHHFLAATPAALNYFESQEQ
CCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCEEEECHHHHHHHHHCCC
NFIHATPFDFSPRLQSSGRNSVEYGQQMIKKAAKGEVVSLSWLHMSQQGKELPTVLTLYP
CEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCEEEEEEC
AQLDNIKVMIVSFKPLDRRKRSRESISNGFETLPKELMSITLEDSAEAVYITDEDDRILA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCEEE
VNKAMCRICGYSAEHLLSKTPSDIKLELQHQNQAPDNPLKGKGSWQGEVWKCRSDGSTFP
HHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCH
AWQSCRRIYADNTVFYVNLFSDISEKKALENKLTQQAMYDKLTGLPNRFHLIQLLNKAIN
HHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
NIKQSPDSIGALMFLDLNGFKNINDSFGHSTGDKVLQLVAARLEACCIENAEIARLGGDE
HHHCCCCCCCEEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
FTLVLPQCHSRQEIEAFSELILSLFEAPFEINGQKFYLGTSIGISLFPQQSDEPNQLLSM
EEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCEEECCCCCCCHHHHHHH
ADTAMYSAKNSPKHIRFYNATIREAAEKKLHTLNDLRHAQSLGQFSLVYQSIVDLESNRT
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VAIEALLRWNQSNGEVIEAHDFIPLLEEAGLMVNVGQWALEQACIQMLKWQQDYDSELKI
EEEEHHHHHCCCCCCEEEECCCHHHHHHCCCEEECHHHHHHHHHHHHHHHCCCCCCCEEE
SVNISRSQLEHPDFISMLEGLLTRTCLPASALIIEITENAFIHRPKLMTKVLAQLKEMGI
EEEECHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECHHHHHHHHHHHHHCCE
CITIDDFGAGLSSLSRLSSLPIDSLKIDAGFAQRLDCNQGRKLCKAIMQLAQTLEINFVA
EEEECCCCCCHHHHHHHHCCCCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHEEEE
EGVETQAQRDMLLSLGKGMGQGYFYNRPTAAENFTHEVLDPIYATTQTKNLAAR
CCHHHHHHHHHHHHHHCCCCCCCEECCCCCHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLLTWSELYPRVSSTDPAHSEQFLLWQNESESTALAILQGHHFLAATPAALNYFESQEQ
CCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCEEEECHHHHHHHHHCCC
NFIHATPFDFSPRLQSSGRNSVEYGQQMIKKAAKGEVVSLSWLHMSQQGKELPTVLTLYP
CEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCEEEEEEC
AQLDNIKVMIVSFKPLDRRKRSRESISNGFETLPKELMSITLEDSAEAVYITDEDDRILA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCEEE
VNKAMCRICGYSAEHLLSKTPSDIKLELQHQNQAPDNPLKGKGSWQGEVWKCRSDGSTFP
HHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCH
AWQSCRRIYADNTVFYVNLFSDISEKKALENKLTQQAMYDKLTGLPNRFHLIQLLNKAIN
HHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
NIKQSPDSIGALMFLDLNGFKNINDSFGHSTGDKVLQLVAARLEACCIENAEIARLGGDE
HHHCCCCCCCEEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
FTLVLPQCHSRQEIEAFSELILSLFEAPFEINGQKFYLGTSIGISLFPQQSDEPNQLLSM
EEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCEEECCCCCCCHHHHHHH
ADTAMYSAKNSPKHIRFYNATIREAAEKKLHTLNDLRHAQSLGQFSLVYQSIVDLESNRT
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VAIEALLRWNQSNGEVIEAHDFIPLLEEAGLMVNVGQWALEQACIQMLKWQQDYDSELKI
EEEEHHHHHCCCCCCEEEECCCHHHHHHCCCEEECHHHHHHHHHHHHHHHCCCCCCCEEE
SVNISRSQLEHPDFISMLEGLLTRTCLPASALIIEITENAFIHRPKLMTKVLAQLKEMGI
EEEECHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECHHHHHHHHHHHHHCCE
CITIDDFGAGLSSLSRLSSLPIDSLKIDAGFAQRLDCNQGRKLCKAIMQLAQTLEINFVA
EEEECCCCCCHHHHHHHHCCCCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHEEEE
EGVETQAQRDMLLSLGKGMGQGYFYNRPTAAENFTHEVLDPIYATTQTKNLAAR
CCHHHHHHHHHHHHHHCCCCCCCEECCCCCHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]