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The map label for this gene is spoT [H]

Identifier: 157963690

GI number: 157963690

Start: 4724143

End: 4726248

Strand: Direct

Name: spoT [H]

Synonym: Spea_3879

Alternate gene names: 157963690

Gene position: 4724143-4726248 (Clockwise)

Preceding gene: 157963689

Following gene: 157963691

Centisome position: 91.3

GC content: 49.1

Gene sequence:

>2106_bases
TTGTATCTGTTTGAAGGTCTCAAGGAGTCTGCCTCAGGTTATTTAGAACCTGAGCAGGTAGAATTACTCAAGCAGGCCTA
TCAGGTGGCGCGTGATGCCCATGAAGGTCAGATGCGCACGAGTGGCGAACCTTATATTACCCACCCGGTTGCGGTTGCCC
GCATCCTGGCCGATATGCGTCTCGATCATGAGACGCTTATGGCCGCTCTCTTGCACGACACTATCGAAGACACCCCAGTA
ACCAAAGAAGAGCTGGCTGAAATATTCAGCGAGTCTATTGCTGAATTGGTTGAAGGCGTCTCCAAGCTCGATAAACTCAA
ATTTCGCGATAAGAAAGAAGCTCAAGCCGAAAACTTCCGCAAAATGATGATGGCGATGACCCAAGATATTCGAGTCATCT
TAATCAAGCTTGCCGATAGAACCCACAATATGCGCACCTTAGGTGCTCTACGGCCTGATAAGCGCCGTCGCATCGCCCGA
GAAACCCTCGAAATTTACGCCCCGATCGCCAACCGCCTTGGTATTCATAATATTAAGAGTGAGTTAGAGGAATTAGGTTT
TCAAGCCTACTACCCAATGCGATATCGTGTGCTTAGAGAGGTGGTCAAAGCCGCTCGTGGTAATCGTAAAGAGCTTATCC
TAAGCATTGAAGGCGCGATCAATACTCGCCTTGAAGATACTGGCCTAGAGGGCACAGTAAAGGGACGCGAGAAGAACCTT
TATTCCATCTACAACAAGATGCGTAATAAAGAGCTGCAGTTCCAAGAAGTCATGGATATCTATGCCTTTAGAGTCATCGT
TGACTCCATCGATACCTGTTATCGCGTCATGGGCGCCATGCATGGCCTTTATAAGCCACATCCTGGTCGCTTCAAAGATT
ATATCGCTATCCCTAAAGCTAACGGCTATCAGTCACTGCATACTTCGCTTTTTGGCCCTCACGGTGTACCGGTTGAGATC
CAAATTCGTACCGAAGATATGGACCAGATGGCAGATAAAGGGGTTGCGGCTCACTGGCTGTACAAAAAAGGCAGCTCAGC
CGAGCAAGGCACCACCACTCAGGTACGTGCTCGTAAGTGGATGCAAAGCTTGTTGGAACTCCAACAAAGCGCGAGTACCT
CTTTCGAATTTGTTGAAAACTTTAAGACTGAGCTCTTCCCTGAAGAGATCTACGTGTTCACCCCTGAAGGCCGTATTTTA
GAGCTACCAGTGGGCGCTACCCCGGTCGACTTCGCTTACGAAGTCCACACCGACGTGGGCAATACCTGCGTAGGTGCCAA
GGTAAATCGTCAAGCTTACCCGCTCAGTCAACCACTGATCTCCGGTCAGACCGTAGAGATCATTACCGCAAATGGCGCCC
GTCCAAATGCGGCCTGGCTCAACTTTGTAGTGACAGGTAAGGCACGAGCTAAAATCCGTCAGCTACTGAAAAGCCTGAAA
GAAGATGATGCCGTACTACTCGGTAAGCGACTGCTTAATCATGCGCTCGGTGAAACTAAGTTAGATGGCTTATCTCAAGA
GCAGATTGATAAGGTCGTTCGTGATACCAAGCATGACTCACTCAACTCGCTACTTGCCGATATTGGCCTAGGCAATGCCA
TGAGCATTGTTATCGCACAGCGACTGCTTGGGGATCAGCTCCATACAGAAGAGCACAAAGAAGTGCCTACCATGTCTATT
CGTGGCGCAGAAGGCATGCTAGTGACCTTCGCTAACTGCTGTCGTCCAATTCCAGGTGACGCGGTGATTGCCCATGTAAG
CCCAGGTAAAGGCCTAGTGGTGCACATGGAAAGCTGCGCTAACATTCGTGGCTATCAGGGTGAGCCAGACAAGTACATAC
CTGTACAGTGGGATAGTGCTGAAGGCGTCGAGTACCAAGCTAACCTTAGGGTTGAAATTGTTAACCACCAAGGTGCATTA
GCCAAGATCACCTCGATTGTAGCCTCTGCAGGTTCAAACATTCATAACTTAACGACTGAAGAGCGTGATGGGCGAGTTTT
CCTCATCAACCTACGTATTTCAGTGCGAGACCGCATTCATTTGGCTAACGTGATGCGTCGGATCAGAGTACTTCCAGAGG
TACTTCGTACATCGAGAAACCGTTAA

Upstream 100 bases:

>100_bases
GATGAGCGCCAAACAGTTCGTGAACGTGAAGCAGCTGAAATCGCTGCTGTTGCTGCCATCGCTGAAGGCCGCAACAACGT
CATATAAGGAGTAGAGTCAC

Downstream 100 bases:

>100_bases
TTTACTCAATAAAGACAAGGAAAGATCATGGCAGAGAAAATAATCATCGCGACCGCTAAAGCGCCACAAGCAATTGGCAC
TTACTCTCAAGCGGTTAAAG

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQVARDAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTPV
TKEELAEIFSESIAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKSELEELGFQAYYPMRYRVLREVVKAARGNRKELILSIEGAINTRLEDTGLEGTVKGREKNL
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKWMQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRIL
ELPVGATPVDFAYEVHTDVGNTCVGAKVNRQAYPLSQPLISGQTVEIITANGARPNAAWLNFVVTGKARAKIRQLLKSLK
EDDAVLLGKRLLNHALGETKLDGLSQEQIDKVVRDTKHDSLNSLLADIGLGNAMSIVIAQRLLGDQLHTEEHKEVPTMSI
RGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCANIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGAL
AKITSIVASAGSNIHNLTTEERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR

Sequences:

>Translated_701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQVARDAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTPV
TKEELAEIFSESIAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKSELEELGFQAYYPMRYRVLREVVKAARGNRKELILSIEGAINTRLEDTGLEGTVKGREKNL
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKWMQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRIL
ELPVGATPVDFAYEVHTDVGNTCVGAKVNRQAYPLSQPLISGQTVEIITANGARPNAAWLNFVVTGKARAKIRQLLKSLK
EDDAVLLGKRLLNHALGETKLDGLSQEQIDKVVRDTKHDSLNSLLADIGLGNAMSIVIAQRLLGDQLHTEEHKEVPTMSI
RGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCANIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGAL
AKITSIVASAGSNIHNLTTEERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
>Mature_701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQVARDAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTPV
TKEELAEIFSESIAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKSELEELGFQAYYPMRYRVLREVVKAARGNRKELILSIEGAINTRLEDTGLEGTVKGREKNL
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKWMQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRIL
ELPVGATPVDFAYEVHTDVGNTCVGAKVNRQAYPLSQPLISGQTVEIITANGARPNAAWLNFVVTGKARAKIRQLLKSLK
EDDAVLLGKRLLNHALGETKLDGLSQEQIDKVVRDTKHDSLNSLLADIGLGNAMSIVIAQRLLGDQLHTEEHKEVPTMSI
RGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCANIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGAL
AKITSIVASAGSNIHNLTTEERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=706, Percent_Identity=68.5552407932011, Blast_Score=1017, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=701, Percent_Identity=30.9557774607703, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 78901; Mature: 78901

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFEGLKESASGYLEPEQVELLKQAYQVARDAHEGQMRTSGEPYITHPVAVARILADMR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTPVTKEELAEIFSESIAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELEELGFQAYYPMRYRVLREVVKAARGNRKELILSIEGAINTRLEDTGLEGTVKGREKNL
HHHHCCCCEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEECCCHHHH
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEHCCCCCCCCCCCHHHHHHHHH
MQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRILELPVGATPVDFAYEVHTDVG
HHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCEEEEECCCC
NTCVGAKVNRQAYPLSQPLISGQTVEIITANGARPNAAWLNFVVTGKARAKIRQLLKSLK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHC
EDDAVLLGKRLLNHALGETKLDGLSQEQIDKVVRDTKHDSLNSLLADIGLGNAMSIVIAQ
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
RLLGDQLHTEEHKEVPTMSIRGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCA
HHHHHHHCCHHHHCCCCEEECCCCCCEEEHHHHCCCCCCCEEEEEECCCCCEEEEEHHHC
NIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGALAKITSIVASAGSNIHNLTTE
CCCCCCCCCCCEEEEEECCCCCCEEEECEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLFEGLKESASGYLEPEQVELLKQAYQVARDAHEGQMRTSGEPYITHPVAVARILADMR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTPVTKEELAEIFSESIAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELEELGFQAYYPMRYRVLREVVKAARGNRKELILSIEGAINTRLEDTGLEGTVKGREKNL
HHHHCCCCEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEECCCHHHH
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEHCCCCCCCCCCCHHHHHHHHH
MQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRILELPVGATPVDFAYEVHTDVG
HHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCEEEEECCCC
NTCVGAKVNRQAYPLSQPLISGQTVEIITANGARPNAAWLNFVVTGKARAKIRQLLKSLK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHC
EDDAVLLGKRLLNHALGETKLDGLSQEQIDKVVRDTKHDSLNSLLADIGLGNAMSIVIAQ
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
RLLGDQLHTEEHKEVPTMSIRGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCA
HHHHHHHCCHHHHCCCCEEECCCCCCEEEHHHHCCCCCCCEEEEEECCCCCEEEEEHHHC
NIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGALAKITSIVASAGSNIHNLTTE
CCCCCCCCCCCEEEEEECCCCCCEEEECEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]