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The map label for this gene is oprF [H]

Identifier: 157963679

GI number: 157963679

Start: 4702004

End: 4703218

Strand: Reverse

Name: oprF [H]

Synonym: Spea_3868

Alternate gene names: 157963679

Gene position: 4703218-4702004 (Counterclockwise)

Preceding gene: 157963680

Following gene: 157963677

Centisome position: 90.89

GC content: 46.5

Gene sequence:

>1215_bases
ATGAAAACTTATCCGTTTTGCTCGTCTTCAAGCGTTTTAATTACAGCACTACTTTGCCCTCTAGTGGTACAAGCCGCGGA
AAATGAGTCGCAACAAACCGAGCATATGTGGTCTCAGGGTTGGTATCTCGGTGGACAGTTTGGTTTAGCGACAACCAATG
TGTCTAACGCGGGATTAGATGAGCTATATGAGCAAGCAGGGATCGATGCGAGTAGCACTAAAGTTGATGATTCTGGAGCG
AGTTATGGTCTATTTCTAGGTTATAAGTTCAATCAATATTTTAGTGTGGAAGCGGGATACCTTGATTTGGGCGAGCGTAG
CGTCGAATTTTCTGGGCAAACAACCGATTTAGATGCTTATTATGATTTAGCCGAGCATGTATACCCTGAAACCGGTGATG
GCTGGTCCTTGAGTGTATTGGGGACCTATCCCCTCAGCGAACGCTTCAGTGTTACAGGCAAGCTGGGTTATTTTGCTTGG
GAGTTAGATGCCGTCACCTCTTCGATTGCAGATGAGGCCAGTCAAGTAGGCTCAGACTCCCACTCTGGCTCTGGAGTTTG
GTTGGGCGCCGAGCTAGGTTATCAAATTAATCATGATATGCAGGCCTATGTTAGCTATCAGCATATGCCACTTGATGCCG
ATGAAGTCGGGGTGTTTGCTTTAGGTCTTAGATACTGGTTTGGCTCTGATTCTCGTGATGCCGCGCCAGTTTTACCTGCC
GCAGTCGTGCCGACGCTTGCTAAAATTGGTAGTGATGGTGATAGTGATAGTGATGGCGTGTTTAACGCGCAAGATCAGTG
TCTAGATACGCCGAGCACTCATGCGGTAGATAGCCGAGGCTGCACTCTGTTTGCCCCGAGAGTGGTCGAGATGAAGCTAA
CAGTCTTGTATGAGAATGACAGCGATAAGATCGACCTAAGCAACACAGATAAAATCCAAAAGCTAGCCGACTTTATCGAG
CAATATGATATTAAACAGATCACAGTATTTGGTCATACATCGGCTGTAGGGTCTCAAGCATACAATCAAAAATTATCTGA
GCGCCGCGCCGCATCTGTGGCTGAAATGCTAGCGGCAGACTTTAATATCGCTACAGGTATCATCAAAGCCGTGGGTAAGG
GGGAGTCTGAACCTATCAGCCATATCCCAGAACAAAATCGACGCATTGAGGTTTATCTTAACGAGGAACTCAATCTACCA
GTGTTAAAGCAATGA

Upstream 100 bases:

>100_bases
ATCCTATTTATGTTTGTTTGCCTATATCGCCGTAACGGCTTTTTACTCAGTAGAGCAAAGCATCTCCTCTTAGTATCAGA
TAGTAAGGATTAGGATATTT

Downstream 100 bases:

>100_bases
TTTAAGTGAGAGCCTTGGGGCTGTTTAGGGCTTAATCATAAATAGCAGTGAACCTAATACGCCGGCGATACTCTCTCCGG
CTATGCAGCCTGCCGCTAGG

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKTYPFCSSSSVLITALLCPLVVQAAENESQQTEHMWSQGWYLGGQFGLATTNVSNAGLDELYEQAGIDASSTKVDDSGA
SYGLFLGYKFNQYFSVEAGYLDLGERSVEFSGQTTDLDAYYDLAEHVYPETGDGWSLSVLGTYPLSERFSVTGKLGYFAW
ELDAVTSSIADEASQVGSDSHSGSGVWLGAELGYQINHDMQAYVSYQHMPLDADEVGVFALGLRYWFGSDSRDAAPVLPA
AVVPTLAKIGSDGDSDSDGVFNAQDQCLDTPSTHAVDSRGCTLFAPRVVEMKLTVLYENDSDKIDLSNTDKIQKLADFIE
QYDIKQITVFGHTSAVGSQAYNQKLSERRAASVAEMLAADFNIATGIIKAVGKGESEPISHIPEQNRRIEVYLNEELNLP
VLKQ

Sequences:

>Translated_404_residues
MKTYPFCSSSSVLITALLCPLVVQAAENESQQTEHMWSQGWYLGGQFGLATTNVSNAGLDELYEQAGIDASSTKVDDSGA
SYGLFLGYKFNQYFSVEAGYLDLGERSVEFSGQTTDLDAYYDLAEHVYPETGDGWSLSVLGTYPLSERFSVTGKLGYFAW
ELDAVTSSIADEASQVGSDSHSGSGVWLGAELGYQINHDMQAYVSYQHMPLDADEVGVFALGLRYWFGSDSRDAAPVLPA
AVVPTLAKIGSDGDSDSDGVFNAQDQCLDTPSTHAVDSRGCTLFAPRVVEMKLTVLYENDSDKIDLSNTDKIQKLADFIE
QYDIKQITVFGHTSAVGSQAYNQKLSERRAASVAEMLAADFNIATGIIKAVGKGESEPISHIPEQNRRIEVYLNEELNLP
VLKQ
>Mature_404_residues
MKTYPFCSSSSVLITALLCPLVVQAAENESQQTEHMWSQGWYLGGQFGLATTNVSNAGLDELYEQAGIDASSTKVDDSGA
SYGLFLGYKFNQYFSVEAGYLDLGERSVEFSGQTTDLDAYYDLAEHVYPETGDGWSLSVLGTYPLSERFSVTGKLGYFAW
ELDAVTSSIADEASQVGSDSHSGSGVWLGAELGYQINHDMQAYVSYQHMPLDADEVGVFALGLRYWFGSDSRDAAPVLPA
AVVPTLAKIGSDGDSDSDGVFNAQDQCLDTPSTHAVDSRGCTLFAPRVVEMKLTVLYENDSDKIDLSNTDKIQKLADFIE
QYDIKQITVFGHTSAVGSQAYNQKLSERRAASVAEMLAADFNIATGIIKAVGKGESEPISHIPEQNRRIEVYLNEELNLP
VLKQ

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 43969; Mature: 43969

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYPFCSSSSVLITALLCPLVVQAAENESQQTEHMWSQGWYLGGQFGLATTNVSNAGLD
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCEECEEECCCCCCCHH
ELYEQAGIDASSTKVDDSGASYGLFLGYKFNQYFSVEAGYLDLGERSVEFSGQTTDLDAY
HHHHHHCCCCCCCCCCCCCCCEEEEEEEEECEEEEEECCEEECCCCEEECCCCCCCHHHH
YDLAEHVYPETGDGWSLSVLGTYPLSERFSVTGKLGYFAWELDAVTSSIADEASQVGSDS
HHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEECEEEEEEHHHHHHHHHHHHHHCCCCC
HSGSGVWLGAELGYQINHDMQAYVSYQHMPLDADEVGVFALGLRYWFGSDSRDAAPVLPA
CCCCEEEEEEECCEEECCCCEEEEEEECCCCCHHHHHHHEEEEHHHCCCCCCCCCCCCHH
AVVPTLAKIGSDGDSDSDGVFNAQDQCLDTPSTHAVDSRGCTLFAPRVVEMKLTVLYEND
HHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEECCEEEEEEEEEEEECC
SDKIDLSNTDKIQKLADFIEQYDIKQITVFGHTSAVGSQAYNQKLSERRAASVAEMLAAD
CCEEECCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHC
FNIATGIIKAVGKGESEPISHIPEQNRRIEVYLNEELNLPVLKQ
CHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKTYPFCSSSSVLITALLCPLVVQAAENESQQTEHMWSQGWYLGGQFGLATTNVSNAGLD
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCEECEEECCCCCCCHH
ELYEQAGIDASSTKVDDSGASYGLFLGYKFNQYFSVEAGYLDLGERSVEFSGQTTDLDAY
HHHHHHCCCCCCCCCCCCCCCEEEEEEEEECEEEEEECCEEECCCCEEECCCCCCCHHHH
YDLAEHVYPETGDGWSLSVLGTYPLSERFSVTGKLGYFAWELDAVTSSIADEASQVGSDS
HHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEECEEEEEEHHHHHHHHHHHHHHCCCCC
HSGSGVWLGAELGYQINHDMQAYVSYQHMPLDADEVGVFALGLRYWFGSDSRDAAPVLPA
CCCCEEEEEEECCEEECCCCEEEEEEECCCCCHHHHHHHEEEEHHHCCCCCCCCCCCCHH
AVVPTLAKIGSDGDSDSDGVFNAQDQCLDTPSTHAVDSRGCTLFAPRVVEMKLTVLYEND
HHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEECCEEEEEEEEEEEECC
SDKIDLSNTDKIQKLADFIEQYDIKQITVFGHTSAVGSQAYNQKLSERRAASVAEMLAAD
CCEEECCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHC
FNIATGIIKAVGKGESEPISHIPEQNRRIEVYLNEELNLPVLKQ
CHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]