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The map label for this gene is norW [H]

Identifier: 157963463

GI number: 157963463

Start: 4440929

End: 4442098

Strand: Reverse

Name: norW [H]

Synonym: Spea_3651

Alternate gene names: 157963463

Gene position: 4442098-4440929 (Counterclockwise)

Preceding gene: 157963464

Following gene: 157963462

Centisome position: 85.84

GC content: 47.69

Gene sequence:

>1170_bases
ATGATGAGTGAGCAAATTGATGCGCCAATCGTCATCATTGGTAGTGGCTTTGCCGCTTACCAACTTATTAGAGCCATTCG
ACGCAGCGATCAAACCATTAAGATCCAGGTTTTTAGTGCTGATAATGGTGATGATTACAATAAACCAGACTTGAGCCATG
TGGTGTCTAAGCAGCAGTTGGCAAGCGATCTTGTGCGTGCATCAGGCGATGAGCTAGCGCGGGAGCTTAACTTTGAGCTG
CACGCCAATACCTATGTAGACTCGATTGAGCCAGATCAACATAAGATCATGGTTGCAGGGAAGGTGTATCCATACGCAAA
GCTAGTTATTGCAACCGGAGCTAGGGCTTTTGTGCCGCCGCTCTCAGGCAACGCAGTGGATCGCATTGTGACCCTCAATA
GTTTGCAAGAGTATCAAGTTGCCGAGCAAGAATTGGCATTGGCCAAGCGAGTGCTGGTAATAGGCGGCGGTTTGATCGGC
ACCGAGTTATCTATGGATCTCGCAACAGCAGGCAAGCGAGTTATCACTGTCGATCCTTGCGATCAACTGATGGCTAATTT
ACTCCCCGAATACATTGCCACTCAGCTAAGCAAGCAGATGCAGCAAATAGGCGTACAGTTAGCGCTGAATAACAAAGTGA
GTAGCATTAGCTCGAGTGAAGATTGTGGAGCTTTTCAGGTAGAGCTGGCAAATGGTGAGTCGCTTATTGTCGATACTGTC
ATTTCTGCCGCAGGACTTCAGGCTAACACTCAGCTAGCACTGCAAGCGGGTTTGGCGGTTAAGCGAGGCATAGTGGTGGA
TAAGCAAATGCAAACCACGGCAATGGATGTCTATGCCTTAGGCGATTGCGCTGAGATTGGCGGTAAGGTGCTAGCCTATC
TGCAGCCAGCTTTAGTTTGTGCCAATGCATTAGCTAAAACCTTGTTAGGCCAATTAACGGCAGTCGTATTGCCTGCCATG
TTGGTTAAAGTTAAAACGCCGCTTTATCCTATTCAGTTGGCAGGGCAAGCCGTGCAACAGGTTAATCGCTGGCAATTAGA
TATAGATGCACAGGGGACTTGTGTACAAGCATTTGATACTGATGAACAGTTACAAGGTTTTGTGGTTGCAGAGCAGCACA
TGAGTAAGGCTTTTGGTTTGCTTAAGCGTTTAGGAAAAGCGCCTGCCTAA

Upstream 100 bases:

>100_bases
GTGGCCCCCGGCACCACTTGGTCAGATGTAGCGGAAGATTTTCTGTGCCCAGAATGCGGTATTGGTAAACAAGTGTTTCA
ACCCATAGCAGGAGCATAAT

Downstream 100 bases:

>100_bases
GATTGAACGAGTTAACTTAAGCATTAATTAGCTTTGTATCAAATAATCTACATTGCGCTTTTGTGCTATCGAAAACCAGA
AAGTAGAGAGCAAAAATTAG

Product: nitric oxide reductase

Products: NAD; reduced flavorubredoxin; H2O [C]

Alternate protein names: Flavorubredoxin reductase; FlRd-reductase; FlavoRb reductase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MMSEQIDAPIVIIGSGFAAYQLIRAIRRSDQTIKIQVFSADNGDDYNKPDLSHVVSKQQLASDLVRASGDELARELNFEL
HANTYVDSIEPDQHKIMVAGKVYPYAKLVIATGARAFVPPLSGNAVDRIVTLNSLQEYQVAEQELALAKRVLVIGGGLIG
TELSMDLATAGKRVITVDPCDQLMANLLPEYIATQLSKQMQQIGVQLALNNKVSSISSSEDCGAFQVELANGESLIVDTV
ISAAGLQANTQLALQAGLAVKRGIVVDKQMQTTAMDVYALGDCAEIGGKVLAYLQPALVCANALAKTLLGQLTAVVLPAM
LVKVKTPLYPIQLAGQAVQQVNRWQLDIDAQGTCVQAFDTDEQLQGFVVAEQHMSKAFGLLKRLGKAPA

Sequences:

>Translated_389_residues
MMSEQIDAPIVIIGSGFAAYQLIRAIRRSDQTIKIQVFSADNGDDYNKPDLSHVVSKQQLASDLVRASGDELARELNFEL
HANTYVDSIEPDQHKIMVAGKVYPYAKLVIATGARAFVPPLSGNAVDRIVTLNSLQEYQVAEQELALAKRVLVIGGGLIG
TELSMDLATAGKRVITVDPCDQLMANLLPEYIATQLSKQMQQIGVQLALNNKVSSISSSEDCGAFQVELANGESLIVDTV
ISAAGLQANTQLALQAGLAVKRGIVVDKQMQTTAMDVYALGDCAEIGGKVLAYLQPALVCANALAKTLLGQLTAVVLPAM
LVKVKTPLYPIQLAGQAVQQVNRWQLDIDAQGTCVQAFDTDEQLQGFVVAEQHMSKAFGLLKRLGKAPA
>Mature_389_residues
MMSEQIDAPIVIIGSGFAAYQLIRAIRRSDQTIKIQVFSADNGDDYNKPDLSHVVSKQQLASDLVRASGDELARELNFEL
HANTYVDSIEPDQHKIMVAGKVYPYAKLVIATGARAFVPPLSGNAVDRIVTLNSLQEYQVAEQELALAKRVLVIGGGLIG
TELSMDLATAGKRVITVDPCDQLMANLLPEYIATQLSKQMQQIGVQLALNNKVSSISSSEDCGAFQVELANGESLIVDTV
ISAAGLQANTQLALQAGLAVKRGIVVDKQMQTTAMDVYALGDCAEIGGKVLAYLQPALVCANALAKTLLGQLTAVVLPAM
LVKVKTPLYPIQLAGQAVQQVNRWQLDIDAQGTCVQAFDTDEQLQGFVVAEQHMSKAFGLLKRLGKAPA

Specific function: One of at least two accessory proteins for anaerobic nitric oxide (NO) reductase. Reduces the rubredoxin moiety of NO reductase [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI4757732, Length=226, Percent_Identity=25.6637168141593, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI22202629, Length=226, Percent_Identity=25.6637168141593, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI65787454, Length=279, Percent_Identity=24.3727598566308, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI226437568, Length=279, Percent_Identity=24.3727598566308, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI21389617, Length=279, Percent_Identity=24.3727598566308, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1789065, Length=375, Percent_Identity=45.8666666666667, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=29.0322580645161, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789765, Length=326, Percent_Identity=26.0736196319018, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=286, Percent_Identity=24.1258741258741, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639257, Length=278, Percent_Identity=22.6618705035971, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281359715, Length=278, Percent_Identity=22.6618705035971, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281359713, Length=278, Percent_Identity=22.6618705035971, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24639250, Length=278, Percent_Identity=22.6618705035971, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI18543267, Length=278, Percent_Identity=22.6618705035971, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24639252, Length=278, Percent_Identity=22.6618705035971, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 41675; Mature: 41675

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSEQIDAPIVIIGSGFAAYQLIRAIRRSDQTIKIQVFSADNGDDYNKPDLSHVVSKQQL
CCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHH
ASDLVRASGDELARELNFELHANTYVDSIEPDQHKIMVAGKVYPYAKLVIATGARAFVPP
HHHHHHCCHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEECCCCHHEEEEECCCCCCCCC
LSGNAVDRIVTLNSLQEYQVAEQELALAKRVLVIGGGLIGTELSMDLATAGKRVITVDPC
CCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHCCCEEEEECCH
DQLMANLLPEYIATQLSKQMQQIGVQLALNNKVSSISSSEDCGAFQVELANGESLIVDTV
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHCCCCCCCCCEEEEEECCCCEEHHHHH
ISAAGLQANTQLALQAGLAVKRGIVVDKQMQTTAMDVYALGDCAEIGGKVLAYLQPALVC
HHHCCCCCCCHHHHHHCHHHHCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ANALAKTLLGQLTAVVLPAMLVKVKTPLYPIQLAGQAVQQVNRWQLDIDAQGTCVQAFDT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEECCC
DEQLQGFVVAEQHMSKAFGLLKRLGKAPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMSEQIDAPIVIIGSGFAAYQLIRAIRRSDQTIKIQVFSADNGDDYNKPDLSHVVSKQQL
CCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHH
ASDLVRASGDELARELNFELHANTYVDSIEPDQHKIMVAGKVYPYAKLVIATGARAFVPP
HHHHHHCCHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEECCCCHHEEEEECCCCCCCCC
LSGNAVDRIVTLNSLQEYQVAEQELALAKRVLVIGGGLIGTELSMDLATAGKRVITVDPC
CCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHCCCEEEEECCH
DQLMANLLPEYIATQLSKQMQQIGVQLALNNKVSSISSSEDCGAFQVELANGESLIVDTV
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHCCCCCCCCCEEEEEECCCCEEHHHHH
ISAAGLQANTQLALQAGLAVKRGIVVDKQMQTTAMDVYALGDCAEIGGKVLAYLQPALVC
HHHCCCCCCCHHHHHHCHHHHCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ANALAKTLLGQLTAVVLPAMLVKVKTPLYPIQLAGQAVQQVNRWQLDIDAQGTCVQAFDT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEECCC
DEQLQGFVVAEQHMSKAFGLLKRLGKAPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; oxidized flavorubredoxin; O2 [C]

Specific reaction: NADH + oxidized flavorubredoxin + O2 = NAD + reduced flavorubredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA