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The map label for this gene is mlaE [H]

Identifier: 157963422

GI number: 157963422

Start: 4400813

End: 4401598

Strand: Reverse

Name: mlaE [H]

Synonym: Spea_3608

Alternate gene names: 157963422

Gene position: 4401598-4400813 (Counterclockwise)

Preceding gene: 157963423

Following gene: 157963421

Centisome position: 85.06

GC content: 49.75

Gene sequence:

>786_bases
GTGAGCTTGATTGATCATATTGCTCGACTAGGTCGGGCTGCAATTGAGTTAGTTATTGGGCTAGGACAAGCCGGTCTAAT
GCTGTGGGGCGCCATTGTGCATCGCCCAAGATTTCGTAAAGGTACGCCGTTACTGCTAAAGCAGCTGTATGTTATTGGAG
TACAGTCTATGGTGATCATCATAGTCTCTGGGCTGTTTATCGGCATGGTGCTGGCGCTACAAGGCTATAATATCTTAGTA
GGCTTTGGCACTGAGGAGAGCCTGGGCCCCATGGTTGCCCTGAGCTTATTGCGAGAGTTAGGCCCTGTGGTCACCGCACT
GCTATTCGCCGGCCGCGCAGGTTCTGCGTTAACGGCTGAGCTCGGCCTAATGAAGAGTACCGAGCAGTTATCTAGTCTAG
AGATGATGGCTATTGACCCATTGAGGCAGATTATTGCGCCACGCTTTTGGGCTGGTGTTATCAGTTTGCCACTGCTGGCG
CTGATGTTTACCGCAGTCGGGATCTACGGTGGTCACCTTGTTGGTGTCGAGTGGAAAGGCATTGATAGCGGTAGCTTCTG
GTCAATACTTCAGGCATCTGTTGAGTGGCGAGAAGATATCGTTAACTGCATTATCAAGAGTTTGTTATTTGCTGTGGTGG
TGACATGGATTGCGCTTTATCGAGGCTATCATGTGATCCCGAACCCTGAAGGGATTAGCAGGGCAACGACCCAGACGGTT
GTGCAGTCAAGTTTAGCCGTTTTAGCATTAGACTTCTTATTGACAGCGGTAATGTTTGGTAGTTAG

Upstream 100 bases:

>100_bases
TTATCAGATAATCCGCAATTAAAGCAATTTATTGGTGGTGAGCCTGACGGTCCAGTACCGTTTCATTATCCTGCACAAGA
TTACAAGAAGGAACTTATCG

Downstream 100 bases:

>100_bases
TCGATATGACGAGTGTACATACCTAGTCGTTAATTATTATTTAGATTTATTGAATAGAGTGGTAAATAAGTTATGTTGAC
AAGGAAAATTGAGATTCTAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSLIDHIARLGRAAIELVIGLGQAGLMLWGAIVHRPRFRKGTPLLLKQLYVIGVQSMVIIIVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAELGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISLPLLA
LMFTAVGIYGGHLVGVEWKGIDSGSFWSILQASVEWREDIVNCIIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
VQSSLAVLALDFLLTAVMFGS

Sequences:

>Translated_261_residues
MSLIDHIARLGRAAIELVIGLGQAGLMLWGAIVHRPRFRKGTPLLLKQLYVIGVQSMVIIIVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAELGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISLPLLA
LMFTAVGIYGGHLVGVEWKGIDSGSFWSILQASVEWREDIVNCIIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
VQSSLAVLALDFLLTAVMFGS
>Mature_260_residues
SLIDHIARLGRAAIELVIGLGQAGLMLWGAIVHRPRFRKGTPLLLKQLYVIGVQSMVIIIVSGLFIGMVLALQGYNILVG
FGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAELGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISLPLLAL
MFTAVGIYGGHLVGVEWKGIDSGSFWSILQASVEWREDIVNCIIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTVV
QSSLAVLALDFLLTAVMFGS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=67.1814671814672, Blast_Score=356, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28114; Mature: 27983

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIDHIARLGRAAIELVIGLGQAGLMLWGAIVHRPRFRKGTPLLLKQLYVIGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISLPLLALMFTAVGIYGGHLVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
IDSGSFWSILQASVEWREDIVNCIIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VQSSLAVLALDFLLTAVMFGS
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLIDHIARLGRAAIELVIGLGQAGLMLWGAIVHRPRFRKGTPLLLKQLYVIGVQSMVII
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISLPLLALMFTAVGIYGGHLVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
IDSGSFWSILQASVEWREDIVNCIIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VQSSLAVLALDFLLTAVMFGS
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]