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The map label for this gene is dusA [H]

Identifier: 157963384

GI number: 157963384

Start: 4356098

End: 4357084

Strand: Reverse

Name: dusA [H]

Synonym: Spea_3570

Alternate gene names: 157963384

Gene position: 4357084-4356098 (Counterclockwise)

Preceding gene: 157963385

Following gene: 157963383

Centisome position: 84.2

GC content: 47.42

Gene sequence:

>987_bases
ATGTCAGTTAGACCAATCAATCGCACCTTCTCAATCGCTCCCATGCTGGATTGGACCGACAGGCATTACCGCTATTTTGC
GCGTCTGATGTCTTCTCAGACCTTGCTATATACCGAGATGGTAACAACCGGGGCGATTTTGCATGGACGAGGTGATTACC
TAGCTTATAACCAAGAAGAGCACCCGTTAGCATTGCAGCTTGGCGGTTCGAATCCAGTAGATCTTGCAGCCTGTGCCAAG
CTCGCAGCCGAGCGTGGTTACGATGAGATTAATCTTAACGTCGGCTGCCCATCGGATAGAGTGCAGAATGGCCGCTTTGG
CGCCTGCCTGATGGCTGAGCCTGAGTTGGTGGCTGAATGTGTGACGGCAATGAAGCAAGTGGTGGATATTCCGGTTACGG
TTAAGACTCGTATTGGTATCGATGAGCAAGATAGCTATGAGTTTTTAACTCAATTTATCGATACAGTAAAAGCTGCTGGC
TGTGAAGAATTTATTATCCATGCACGTAAGGCTTGGCTTCAAGGTTTGAGTCCGAAAGAGAATCGCGAGATCCCGCCATT
AGATTATGAGCGTGTATATCAACTTAAACGAGATTACCCAGAGTTAAATATCTCGATTAACGGCGGTGTTAAAACGCTAG
AGGAGTGTCAGACTCACTTGCAGCACCTTGACGGAGTAATGGTGGGTCGTGAAGCCTATCAAAATCCATATATTCTTGCA
GAAGTCGATCAGAAGTTATGCGGTATCGATGCGCCGGTGATGAGTCGAGATGCTGTTTTGGAGAAACTACTTCCTTATAT
AGAGAAACATCTGCAGCAAGGCGGACGCCTTAACCATATTTCTCGCCATATTATTGGTCTTTTCCAAGGTGAGGTAGGCT
CCCGTGTATGGCGCCGATATATAAGCGAAAATGCTCATAAGCAAGGCGCTGGTATTGAGGTGATAACTCAGGCGAGAGCC
GAGATGGACTCGGTTGTGAGCCTATAG

Upstream 100 bases:

>100_bases
GCCCTATTTGGGTTAGGCTGAAGTCGGGCATTTTTTCAGATAGGCACGTCACCCTGAATAGGGCAAAAACTGTCTTTATT
TAAAGTTGGATAATTAGTTT

Downstream 100 bases:

>100_bases
TATTAGTTATTTGCATTAGTGATTCCATATTAGAGAATCTATATTAGTGAAATTGGCCAGAGCATGGTGAGTTTCACTAA
TTTCTTTTTATGTTTAAATA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSVRPINRTFSIAPMLDWTDRHYRYFARLMSSQTLLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPVDLAACAK
LAAERGYDEINLNVGCPSDRVQNGRFGACLMAEPELVAECVTAMKQVVDIPVTVKTRIGIDEQDSYEFLTQFIDTVKAAG
CEEFIIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPELNISINGGVKTLEECQTHLQHLDGVMVGREAYQNPYILA
EVDQKLCGIDAPVMSRDAVLEKLLPYIEKHLQQGGRLNHISRHIIGLFQGEVGSRVWRRYISENAHKQGAGIEVITQARA
EMDSVVSL

Sequences:

>Translated_328_residues
MSVRPINRTFSIAPMLDWTDRHYRYFARLMSSQTLLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPVDLAACAK
LAAERGYDEINLNVGCPSDRVQNGRFGACLMAEPELVAECVTAMKQVVDIPVTVKTRIGIDEQDSYEFLTQFIDTVKAAG
CEEFIIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPELNISINGGVKTLEECQTHLQHLDGVMVGREAYQNPYILA
EVDQKLCGIDAPVMSRDAVLEKLLPYIEKHLQQGGRLNHISRHIIGLFQGEVGSRVWRRYISENAHKQGAGIEVITQARA
EMDSVVSL
>Mature_327_residues
SVRPINRTFSIAPMLDWTDRHYRYFARLMSSQTLLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPVDLAACAKL
AAERGYDEINLNVGCPSDRVQNGRFGACLMAEPELVAECVTAMKQVVDIPVTVKTRIGIDEQDSYEFLTQFIDTVKAAGC
EEFIIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPELNISINGGVKTLEECQTHLQHLDGVMVGREAYQNPYILAE
VDQKLCGIDAPVMSRDAVLEKLLPYIEKHLQQGGRLNHISRHIIGLFQGEVGSRVWRRYISENAHKQGAGIEVITQARAE
MDSVVSL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=26.271186440678, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI239788483, Length=212, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=239, Percent_Identity=23.4309623430962, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI40807366, Length=238, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=71.6129032258065, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1788462, Length=224, Percent_Identity=29.0178571428571, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=26.4227642276423, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=172, Percent_Identity=30.8139534883721, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=268, Percent_Identity=24.6268656716418, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=24.8945147679325, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24580595, Length=293, Percent_Identity=25.2559726962457, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19920448, Length=293, Percent_Identity=25.2559726962457, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921524, Length=234, Percent_Identity=24.3589743589744, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36918; Mature: 36786

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRPINRTFSIAPMLDWTDRHYRYFARLMSSQTLLYTEMVTTGAILHGRGDYLAYNQEE
CCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCEEEECCCC
HPLALQLGGSNPVDLAACAKLAAERGYDEINLNVGCPSDRVQNGRFGACLMAEPELVAEC
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHHH
VTAMKQVVDIPVTVKTRIGIDEQDSYEFLTQFIDTVKAAGCEEFIIHARKAWLQGLSPKE
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
NREIPPLDYERVYQLKRDYPELNISINGGVKTLEECQTHLQHLDGVMVGREAYQNPYILA
CCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
EVDQKLCGIDAPVMSRDAVLEKLLPYIEKHLQQGGRLNHISRHIIGLFQGEVGSRVWRRY
EHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHH
ISENAHKQGAGIEVITQARAEMDSVVSL
HHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVRPINRTFSIAPMLDWTDRHYRYFARLMSSQTLLYTEMVTTGAILHGRGDYLAYNQEE
CCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCEEEECCCC
HPLALQLGGSNPVDLAACAKLAAERGYDEINLNVGCPSDRVQNGRFGACLMAEPELVAEC
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHHH
VTAMKQVVDIPVTVKTRIGIDEQDSYEFLTQFIDTVKAAGCEEFIIHARKAWLQGLSPKE
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
NREIPPLDYERVYQLKRDYPELNISINGGVKTLEECQTHLQHLDGVMVGREAYQNPYILA
CCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
EVDQKLCGIDAPVMSRDAVLEKLLPYIEKHLQQGGRLNHISRHIIGLFQGEVGSRVWRRY
EHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHH
ISENAHKQGAGIEVITQARAEMDSVVSL
HHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]