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The map label for this gene is parE [H]

Identifier: 157963372

GI number: 157963372

Start: 4345741

End: 4347636

Strand: Reverse

Name: parE [H]

Synonym: Spea_3558

Alternate gene names: 157963372

Gene position: 4347636-4345741 (Counterclockwise)

Preceding gene: 157963373

Following gene: 157963371

Centisome position: 84.02

GC content: 47.26

Gene sequence:

>1896_bases
ATGTGTGCCATGACAAACCAATACACCTCAGATGCTATTGAAGTCCTAAATGGACTCGACCCTGTTAAACGTCGCCCCGG
TATGTATACCGATACTTCGCGCCCAAACCATTTAGGACAGGAAGTCATAGATAATAGTGTCGACGAGGCATTGGCGGGCC
ACGCCAGTCGTATCGACGTTATCTTGCATGCCGATAATTCATTGGAAGTCATCGATGACGGCCGTGGTATGCCTGTCGAT
ATTCACCCTGAAGAGGGGATCCCAGGTGTCGAGCTTATTCTAACTAAGCTGCACGCGGGTGGTAAATTCTCTAATAAAAA
CTATCAGTTTTCAGGTGGTCTTCACGGTGTGGGGATCTCGGTTGTAAACGCGCTATCAAGCCGAGTCGAAATTACCGTTA
GGCGTAATGCTGAAGTGTATGAGATGGCCTTTGAGCACGGTGAAAAAGTCGAAGACTTACATGTGACTGGCACCTGTGGT
CGTCGTAACACAGGCACTAGCGTGCATTTTTGGCCTGATGCGAGTTATTTCGACTCGGGTAATTTCTCGATCTCCAAGCT
GACCTATTTACTTAAAGCTAAGGCCGTACTTTGTCCGGGTCTACGGATTAAATTTGTTAATAAGCAGACCAATGAAACCC
AAGAGTGGTTTTATGAAAGTGGCTTAGAAGACTACTTAAGATCATCCTTCAAAGGTGTTCCAACACTGCCTGAAGTGCCA
TTTGTTGGCAGTTTTGCCGGTAATTTAGAAGCCGCAGATTGGGCGATTACTTGGTTGCCTGAAGGTGGTGAGTATCTCAA
CGAAAGTTATGTAAACCTTATTCCTACGCCGCTTGGTGGTACTCACGTTAACGGTTTTAGACAGGGCTTGCTCGAGTCTA
TGCGTGAATTCTGTGAATTTAGAAACTTGATGCCGCGAGGCATTAAGCTAACCCCAGAAGATATCTGGGATCGCAGCACC
TTTATCTTATCGATCAAGATGCAAGATCCGCAATTTGCAGGCCAAACCAAAGAGAAGCTTTCAAGCAGACAGTGCGCTGC
GTTTGTATCGGGCATTGTACGCGATGCCTTCAGCCTTTGGTTAAACACCAACACCGAACAGGCTGAAGCCTTAGCCGATA
TGTGTATTAATAATGCACAGAAGCGTTTAAAAGCGGCCAAGAAAGTGGCCCGTAAGCGTATTACCTCAGGCCCAGCCCTG
CCGGGTAAGCTGACGGACTGTAGCGGACAAGATCCAATGCGTGGCGAGCTATTTCTAGTGGAGGGTGACTCTGCGGGTGG
TAGTGCTAAGCAGGCTCGCGATCGTGAGTTCCAGGCGATTATGCCGCTACGTGGTAAGATTTTGAATACTTGGGAGGTCG
ATGCCTCTCAGGTGTTAGCTTCACAAGAAGTTCATGATATCTCGGTAGCCATTGGTTGCGACCCAGATAGTGCCGATATT
TCTGAACTGAGATACGGTAAAATCTGTATCCTCGCCGATGCTGACTCGGATGGTTTGCATATTGCGACCTTGCTATGCGC
TTTGTTTATGAAGCATTACCGAGTGCTGGTAGAGCAGGGGCATATCTATGTTGCTATGCCGCCACTGTTCCGTATCGATA
TAGGTAAAGATGTATTTTACGCCCTCGATGAGGCTGAAAAAAACGGTATTCTTGATAGAATAACCGCAGAAAAACGCAAA
GGAAAAGTCCAAGTTACACGATTTAAGGGGCTGGGTGAGATGAACCCATTGCAACTACGTGAAACGACGATGGACCCCAA
TACGCGTCGACTAGTGCAACTTACCATAGATGACGCCGAGGATACTTTGGCGCTTATGGATATGCTTTTAGCCAAGAAAC
GTTCTGGTGACAGAAAAATTTGGCTAGAGACTAAGGGTGATCTCGCCCAGATCTAG

Upstream 100 bases:

>100_bases
TTTATCGGCTATGAGGACCAGCTTGCTTCTATCTGTGAATATCTGTTTTCTTGACAAATAGACCCGTAGGGCCGCAACAT
GGCCTGAATAACAAAATAAT

Downstream 100 bases:

>100_bases
TCGGCTAAAAAGATTATCATCAGACAAAAGAATAATAATGACGTTTGAAAACTATCGAAATCAATTCGCCGTTGGGATCT
CTGCACTTTGCGCTAACTTT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MCAMTNQYTSDAIEVLNGLDPVKRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHASRIDVILHADNSLEVIDDGRGMPVD
IHPEEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSSRVEITVRRNAEVYEMAFEHGEKVEDLHVTGTCG
RRNTGTSVHFWPDASYFDSGNFSISKLTYLLKAKAVLCPGLRIKFVNKQTNETQEWFYESGLEDYLRSSFKGVPTLPEVP
FVGSFAGNLEAADWAITWLPEGGEYLNESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNLMPRGIKLTPEDIWDRST
FILSIKMQDPQFAGQTKEKLSSRQCAAFVSGIVRDAFSLWLNTNTEQAEALADMCINNAQKRLKAAKKVARKRITSGPAL
PGKLTDCSGQDPMRGELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSADI
SELRYGKICILADADSDGLHIATLLCALFMKHYRVLVEQGHIYVAMPPLFRIDIGKDVFYALDEAEKNGILDRITAEKRK
GKVQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEDTLALMDMLLAKKRSGDRKIWLETKGDLAQI

Sequences:

>Translated_631_residues
MCAMTNQYTSDAIEVLNGLDPVKRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHASRIDVILHADNSLEVIDDGRGMPVD
IHPEEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSSRVEITVRRNAEVYEMAFEHGEKVEDLHVTGTCG
RRNTGTSVHFWPDASYFDSGNFSISKLTYLLKAKAVLCPGLRIKFVNKQTNETQEWFYESGLEDYLRSSFKGVPTLPEVP
FVGSFAGNLEAADWAITWLPEGGEYLNESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNLMPRGIKLTPEDIWDRST
FILSIKMQDPQFAGQTKEKLSSRQCAAFVSGIVRDAFSLWLNTNTEQAEALADMCINNAQKRLKAAKKVARKRITSGPAL
PGKLTDCSGQDPMRGELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSADI
SELRYGKICILADADSDGLHIATLLCALFMKHYRVLVEQGHIYVAMPPLFRIDIGKDVFYALDEAEKNGILDRITAEKRK
GKVQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEDTLALMDMLLAKKRSGDRKIWLETKGDLAQI
>Mature_631_residues
MCAMTNQYTSDAIEVLNGLDPVKRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHASRIDVILHADNSLEVIDDGRGMPVD
IHPEEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSSRVEITVRRNAEVYEMAFEHGEKVEDLHVTGTCG
RRNTGTSVHFWPDASYFDSGNFSISKLTYLLKAKAVLCPGLRIKFVNKQTNETQEWFYESGLEDYLRSSFKGVPTLPEVP
FVGSFAGNLEAADWAITWLPEGGEYLNESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNLMPRGIKLTPEDIWDRST
FILSIKMQDPQFAGQTKEKLSSRQCAAFVSGIVRDAFSLWLNTNTEQAEALADMCINNAQKRLKAAKKVARKRITSGPAL
PGKLTDCSGQDPMRGELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSADI
SELRYGKICILADADSDGLHIATLLCALFMKHYRVLVEQGHIYVAMPPLFRIDIGKDVFYALDEAEKNGILDRITAEKRK
GKVQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEDTLALMDMLLAKKRSGDRKIWLETKGDLAQI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=583, Percent_Identity=25.3859348198971, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI19913406, Length=615, Percent_Identity=23.5772357723577, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=74.6031746031746, Blast_Score=1017, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=553, Percent_Identity=38.878842676311, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=644, Percent_Identity=24.0683229813665, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645845, Length=597, Percent_Identity=23.785594639866, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324241, Length=618, Percent_Identity=23.7864077669903, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17136538, Length=596, Percent_Identity=23.1543624161074, Blast_Score=125, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69962; Mature: 69962

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAMTNQYTSDAIEVLNGLDPVKRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHASRIDV
CCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
ILHADNSLEVIDDGRGMPVDIHPEEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGIS
EEECCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSSRVEITVRRNAEVYEMAFEHGEKVEDLHVTGTCGRRNTGTSVHFWPDASYFDSG
HHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCC
NFSISKLTYLLKAKAVLCPGLRIKFVNKQTNETQEWFYESGLEDYLRSSFKGVPTLPEVP
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
FVGSFAGNLEAADWAITWLPEGGEYLNESYVNLIPTPLGGTHVNGFRQGLLESMREFCEF
CCCCCCCCCCCCCEEEEEECCCHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RNLMPRGIKLTPEDIWDRSTFILSIKMQDPQFAGQTKEKLSSRQCAAFVSGIVRDAFSLW
HHCCCCCCEECHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNTNTEQAEALADMCINNAQKRLKAAKKVARKRITSGPALPGKLTDCSGQDPMRGELFLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
EGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSADI
ECCCCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCH
SELRYGKICILADADSDGLHIATLLCALFMKHYRVLVEQGHIYVAMPPLFRIDIGKDVFY
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCHHHH
ALDEAEKNGILDRITAEKRKGKVQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAE
HHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEEECCCH
DTLALMDMLLAKKRSGDRKIWLETKGDLAQI
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MCAMTNQYTSDAIEVLNGLDPVKRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHASRIDV
CCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
ILHADNSLEVIDDGRGMPVDIHPEEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGIS
EEECCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSSRVEITVRRNAEVYEMAFEHGEKVEDLHVTGTCGRRNTGTSVHFWPDASYFDSG
HHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCC
NFSISKLTYLLKAKAVLCPGLRIKFVNKQTNETQEWFYESGLEDYLRSSFKGVPTLPEVP
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
FVGSFAGNLEAADWAITWLPEGGEYLNESYVNLIPTPLGGTHVNGFRQGLLESMREFCEF
CCCCCCCCCCCCCEEEEEECCCHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RNLMPRGIKLTPEDIWDRSTFILSIKMQDPQFAGQTKEKLSSRQCAAFVSGIVRDAFSLW
HHCCCCCCEECHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNTNTEQAEALADMCINNAQKRLKAAKKVARKRITSGPALPGKLTDCSGQDPMRGELFLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
EGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSADI
ECCCCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCH
SELRYGKICILADADSDGLHIATLLCALFMKHYRVLVEQGHIYVAMPPLFRIDIGKDVFY
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCHHHH
ALDEAEKNGILDRITAEKRKGKVQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAE
HHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEEECCCH
DTLALMDMLLAKKRSGDRKIWLETKGDLAQI
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]