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The map label for this gene is yafC [C]

Identifier: 157963214

GI number: 157963214

Start: 4154263

End: 4155177

Strand: Reverse

Name: yafC [C]

Synonym: Spea_3400

Alternate gene names: 157963214

Gene position: 4155177-4154263 (Counterclockwise)

Preceding gene: 157963222

Following gene: 157963210

Centisome position: 80.3

GC content: 41.64

Gene sequence:

>915_bases
ATGAAGCCGAGTTTTAATCTAAATTTGCTAAGTAGCTTTTATGCGGTTGCTCGTCATGGCTCGTTTGCAGCTGCAGCCGA
TGAGTTGTGCTTAACTCATTCTGTGATCAGTAAACATATCAAGCAACTGGAAACGAGTTTACAGAGTCAGTTGATTATCC
GTACCACGCGCGCACTGGAATTAACCGAAGAGGGAGAGTTTCTCTATTCAAAGTGCCAGTCGATTTTTAACGAAGTCTTG
CAAGTTAAAGACTTCATTGATGAAAAGAAGCGGGTTGTCAGTGGGCGTTTAGTCATTAAGATGCCAAGCATTTTAAAGCA
CGATGATGGTATCGCTAAAAGCCTTGAATTTATGCATTTAACCTATCCAGGTTTAATGCTGGATATTGTATTTGACGATA
ATCTTGGTGATTTAGTTAAAGAAGGGGTCGATATTGCCTTTCGTATCGGCAAGCTTGAAGACAGCAGTTATCTATGTAGA
AAAGTCCGCGATATTGACACCTATGTTGTGGCGTCTCCGGAATACTTAAAAGTAGCGGGTACGCCGCTTCATCCTAATGA
GTTAACTCAGCACAAGTGTCTGCACTATAGCCATTGCCTCACAGGCCTCAATTGGTCGTTTAAACAAAATGGTGAGCAAT
TTAATGTGCCGATAACTCCTGCATTATCTTCAATGAGTGAATCCATGTTGGCGCAATATGCTTGTAAAGGATTTGGGGTG
ACCACCACCCTAGACTTTGTGACGGCTAAAGATATTGAGTCGGCGCGATTAGTTAGCCTATTAAAAGATTTTACTTGGAA
AACTGAGTTATATCTTGTCTTTCCCTATGCGGCGGTTGTGCCACTAAAGACACGAAGCTTTATCAATAGTATTTTATCAA
CAAGGGATAATAGCCCAGAAAGCAACCTGGGCTAG

Upstream 100 bases:

>100_bases
ACAATTTCACATATTGAACAATATTCTTTCCAATAAAGCATGAATGCCTAAGTAACGGAGTTAGAATGTAAAATGACTGA
CTGGCACAGAAGAGAAAAAG

Downstream 100 bases:

>100_bases
AAGTGGCTGCTTACTTTGGCTAGTGATATTGGATTAAAGTGGAGGTGATCGCGATATTAAGGTCGATTACCTCCACGGTA
TATTTGTTAACTTGTGTCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKPSFNLNLLSSFYAVARHGSFAAAADELCLTHSVISKHIKQLETSLQSQLIIRTTRALELTEEGEFLYSKCQSIFNEVL
QVKDFIDEKKRVVSGRLVIKMPSILKHDDGIAKSLEFMHLTYPGLMLDIVFDDNLGDLVKEGVDIAFRIGKLEDSSYLCR
KVRDIDTYVVASPEYLKVAGTPLHPNELTQHKCLHYSHCLTGLNWSFKQNGEQFNVPITPALSSMSESMLAQYACKGFGV
TTTLDFVTAKDIESARLVSLLKDFTWKTELYLVFPYAAVVPLKTRSFINSILSTRDNSPESNLG

Sequences:

>Translated_304_residues
MKPSFNLNLLSSFYAVARHGSFAAAADELCLTHSVISKHIKQLETSLQSQLIIRTTRALELTEEGEFLYSKCQSIFNEVL
QVKDFIDEKKRVVSGRLVIKMPSILKHDDGIAKSLEFMHLTYPGLMLDIVFDDNLGDLVKEGVDIAFRIGKLEDSSYLCR
KVRDIDTYVVASPEYLKVAGTPLHPNELTQHKCLHYSHCLTGLNWSFKQNGEQFNVPITPALSSMSESMLAQYACKGFGV
TTTLDFVTAKDIESARLVSLLKDFTWKTELYLVFPYAAVVPLKTRSFINSILSTRDNSPESNLG
>Mature_304_residues
MKPSFNLNLLSSFYAVARHGSFAAAADELCLTHSVISKHIKQLETSLQSQLIIRTTRALELTEEGEFLYSKCQSIFNEVL
QVKDFIDEKKRVVSGRLVIKMPSILKHDDGIAKSLEFMHLTYPGLMLDIVFDDNLGDLVKEGVDIAFRIGKLEDSSYLCR
KVRDIDTYVVASPEYLKVAGTPLHPNELTQHKCLHYSHCLTGLNWSFKQNGEQFNVPITPALSSMSESMLAQYACKGFGV
TTTLDFVTAKDIESARLVSLLKDFTWKTELYLVFPYAAVVPLKTRSFINSILSTRDNSPESNLG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=30.3754266211604, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI87081978, Length=271, Percent_Identity=31.7343173431734, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=27.8350515463918, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=28.0141843971631, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789639, Length=192, Percent_Identity=33.8541666666667, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=25.2525252525253, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=30, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788706, Length=266, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786508, Length=123, Percent_Identity=34.9593495934959, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787589, Length=276, Percent_Identity=23.9130434782609, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=29.0657439446367, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786713, Length=159, Percent_Identity=30.8176100628931, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34128; Mature: 34128

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSFNLNLLSSFYAVARHGSFAAAADELCLTHSVISKHIKQLETSLQSQLIIRTTRALE
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LTEEGEFLYSKCQSIFNEVLQVKDFIDEKKRVVSGRLVIKMPSILKHDDGIAKSLEFMHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCCHHHHHHHHEE
TYPGLMLDIVFDDNLGDLVKEGVDIAFRIGKLEDSSYLCRKVRDIDTYVVASPEYLKVAG
ECCCEEEEEEECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCEEEEEC
TPLHPNELTQHKCLHYSHCLTGLNWSFKQNGEQFNVPITPALSSMSESMLAQYACKGFGV
CCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCC
TTTLDFVTAKDIESARLVSLLKDFTWKTELYLVFPYAAVVPLKTRSFINSILSTRDNSPE
EEEEHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECHHHHCCHHHHHHHHHHHHCCCCCCC
SNLG
CCCC
>Mature Secondary Structure
MKPSFNLNLLSSFYAVARHGSFAAAADELCLTHSVISKHIKQLETSLQSQLIIRTTRALE
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LTEEGEFLYSKCQSIFNEVLQVKDFIDEKKRVVSGRLVIKMPSILKHDDGIAKSLEFMHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCCHHHHHHHHEE
TYPGLMLDIVFDDNLGDLVKEGVDIAFRIGKLEDSSYLCRKVRDIDTYVVASPEYLKVAG
ECCCEEEEEEECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCEEEEEC
TPLHPNELTQHKCLHYSHCLTGLNWSFKQNGEQFNVPITPALSSMSESMLAQYACKGFGV
CCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCC
TTTLDFVTAKDIESARLVSLLKDFTWKTELYLVFPYAAVVPLKTRSFINSILSTRDNSPE
EEEEHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECHHHHCCHHHHHHHHHHHHCCCCCCC
SNLG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]