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The map label for this gene is btuF [H]

Identifier: 157963189

GI number: 157963189

Start: 4121391

End: 4122227

Strand: Reverse

Name: btuF [H]

Synonym: Spea_3374

Alternate gene names: 157963189

Gene position: 4122227-4121391 (Counterclockwise)

Preceding gene: 157963190

Following gene: 157963187

Centisome position: 79.66

GC content: 51.73

Gene sequence:

>837_bases
ATGAACAGAGTTACCAGATTATTTATGTTACTGACGGCTTGTGCTTTACCATCAGCTTTTGCGCATGCTGATATCGCTGC
GCCCGCTAAACGTATTATCGCGCTTTCACCACACTCGGTTGAGATGCTGTATGCCATTGGCGCGGGGGAGGCGATTGTGG
CGACCACAGATCATGCTGATTTCCCCGAGGCAGCTCTCGCCATTCCGCGTATTGGCGGATATCACGGGGTGCAAGTCGAG
CGCATACTGGAGCTAGAGCCCGATCTCGTGGTGGTATGGGGCAGTGGCAATAAAGTCGAAGACATTACCCGTATTAAAGC
GCTAGGTTTTCGGGTGTTCGATAGTAACCCTGACACGCTCGATGAGGTGGCCGATGAGCTTGAGTCTCTGGGAGAGCTAA
CTGGGCATCAAGTCCTGGCTGCAAAAGTGGCTGCCGATTACCGTCAGCAACTTAAGCAATTACGTAGCGAGAATCTTGCC
AAGTCAGGGGTGAATGTCTTCTATCAACTCTGGTCGACCCCGCTTATGACCGTCGCCAAGGATAGCTGGATCCAGCAAAT
CATTTCAGTGTGTCACGGTGATAACGTCTTTGCTGATGCGGCTAACGATTACCCACAAGTCAGCTTAGAAACTGTGGTGC
TTAAGCAGCCCGAAGTGATCTTACAGAGCCAAGATGAAGGCAACATCTTAGGTGTAGACTGGAGCGCGTGGCCTGAGATC
CCTGCGGTTAAAAATAAGCATATTTATCAGCTTAACGCCGACCTACTCCACCGCGCGGCCCCTAGAGCCTTGCTTGGCGT
AGAGGCGCTATGTGATGCGTTAGATAAGGCGCGTTAA

Upstream 100 bases:

>100_bases
TTTCGTATTTGCTGTTATCGGCTTTTCCGTCTTTTCCGTCTTATCCGGATCCGTCTCAGCTTTTACCGTCTTAGATTTAA
CCGACCTTACAAGGATTTTA

Downstream 100 bases:

>100_bases
GAAAATATAGCTAGTGCTTAATCTGTATTTCTGAAAGCGAAGCCTAGCTTCCAATCATTTTAATAGAGCTGGGGTAAGCT
TTTCCTTTCGTTTGACTGTT

Product: periplasmic-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNRVTRLFMLLTACALPSAFAHADIAAPAKRIIALSPHSVEMLYAIGAGEAIVATTDHADFPEAALAIPRIGGYHGVQVE
RILELEPDLVVVWGSGNKVEDITRIKALGFRVFDSNPDTLDEVADELESLGELTGHQVLAAKVAADYRQQLKQLRSENLA
KSGVNVFYQLWSTPLMTVAKDSWIQQIISVCHGDNVFADAANDYPQVSLETVVLKQPEVILQSQDEGNILGVDWSAWPEI
PAVKNKHIYQLNADLLHRAAPRALLGVEALCDALDKAR

Sequences:

>Translated_278_residues
MNRVTRLFMLLTACALPSAFAHADIAAPAKRIIALSPHSVEMLYAIGAGEAIVATTDHADFPEAALAIPRIGGYHGVQVE
RILELEPDLVVVWGSGNKVEDITRIKALGFRVFDSNPDTLDEVADELESLGELTGHQVLAAKVAADYRQQLKQLRSENLA
KSGVNVFYQLWSTPLMTVAKDSWIQQIISVCHGDNVFADAANDYPQVSLETVVLKQPEVILQSQDEGNILGVDWSAWPEI
PAVKNKHIYQLNADLLHRAAPRALLGVEALCDALDKAR
>Mature_278_residues
MNRVTRLFMLLTACALPSAFAHADIAAPAKRIIALSPHSVEMLYAIGAGEAIVATTDHADFPEAALAIPRIGGYHGVQVE
RILELEPDLVVVWGSGNKVEDITRIKALGFRVFDSNPDTLDEVADELESLGELTGHQVLAAKVAADYRQQLKQLRSENLA
KSGVNVFYQLWSTPLMTVAKDSWIQQIISVCHGDNVFADAANDYPQVSLETVVLKQPEVILQSQDEGNILGVDWSAWPEI
PAVKNKHIYQLNADLLHRAAPRALLGVEALCDALDKAR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=250, Percent_Identity=30.4, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30364; Mature: 30364

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVTRLFMLLTACALPSAFAHADIAAPAKRIIALSPHSVEMLYAIGAGEAIVATTDHAD
CCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHEEEECCCCEEEEEEECCCCEEEEECCCCC
FPEAALAIPRIGGYHGVQVERILELEPDLVVVWGSGNKVEDITRIKALGFRVFDSNPDTL
CCHHHHHCCCCCCCCCCHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHEEEECCCCCHH
DEVADELESLGELTGHQVLAAKVAADYRQQLKQLRSENLAKSGVNVFYQLWSTPLMTVAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DSWIQQIISVCHGDNVFADAANDYPQVSLETVVLKQPEVILQSQDEGNILGVDWSAWPEI
HHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCHHEEECCCCCCEEEECCCCCCCC
PAVKNKHIYQLNADLLHRAAPRALLGVEALCDALDKAR
CCCCCCEEEEECHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRVTRLFMLLTACALPSAFAHADIAAPAKRIIALSPHSVEMLYAIGAGEAIVATTDHAD
CCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHEEEECCCCEEEEEEECCCCEEEEECCCCC
FPEAALAIPRIGGYHGVQVERILELEPDLVVVWGSGNKVEDITRIKALGFRVFDSNPDTL
CCHHHHHCCCCCCCCCCHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHEEEECCCCCHH
DEVADELESLGELTGHQVLAAKVAADYRQQLKQLRSENLAKSGVNVFYQLWSTPLMTVAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DSWIQQIISVCHGDNVFADAANDYPQVSLETVVLKQPEVILQSQDEGNILGVDWSAWPEI
HHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCHHEEECCCCCCEEEECCCCCCCC
PAVKNKHIYQLNADLLHRAAPRALLGVEALCDALDKAR
CCCCCCEEEEECHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA