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The map label for this gene is kinB [H]

Identifier: 157963175

GI number: 157963175

Start: 4102730

End: 4104094

Strand: Reverse

Name: kinB [H]

Synonym: Spea_3360

Alternate gene names: 157963175

Gene position: 4104094-4102730 (Counterclockwise)

Preceding gene: 157963176

Following gene: 157963172

Centisome position: 79.31

GC content: 44.69

Gene sequence:

>1365_bases
ATGAGCAATACAGTGGTTTCTCTTAGAGTTAAGCTTATTATCAGCGCAGTGCTCGCTTCAGCTACGGGCTTGTTGCTGGC
ATTCACAGGCCTGTTATGGCTGTCACCTGTAGCGGTGCAGCAAAATGTTGTAGAGCAATCCTGGCTCTACTTGCTGTTAT
TTACTCTGTCTTTGCTGTTAGTAACCAGTATTAGTTACATCGGCACTCGCGACTTAGCGACGAGTTTAAGAGCGTTAGAG
GTCGGCTTACTTAACTTTAAAGATAATGACTTTAGCGTGAGTATACCTGTCAGTGGTGACAGCCAATTAAGAGCATTATC
GACTCTGTTTAATGAGTCGGCGCAGACATTGCGTAAAGAGCGGCAGTACATTTATCAACGTGAATTGCTGCTCGACAAAG
TCATACAAAGCTCGCCTAACGTGATGCTGCTACTCGATGATGAACAGCGGGTCATCTATGCCAATGATGCGGCGAGACAT
TTGTTTAATAGCGGTCAACGTGTTGAAGGTATGAGCCTATCGCAATTGTTGACAGATGCCCCTGAATCTTTGAGTGAGGC
ACTTAATAATCCTAAAGATGGTCTGTTTTCGCTAAAGCATGATGCTGGTGAGAGTGATCTAGAAACTTGGCACTTGTCCC
GAGGCCAGTTTCTGTTAAACGGTCAGTTTCATCACTTGGTGTTACTAAAGCAGTTGACTAGAGAGCTAAATAGGCAGGAG
GTGGCAGTATGGAAGAAGGTGATTCGCATCATCAGTCATGAGCTGAATAACTCGGTTGCACCAATAGCTTCTATGGTTAA
CTCTGGGCGAATGATCACCAAAGAGCTCAACGATCCAAAACTGAAGCTTATTTTCGATACCATCGAAGATAGAAGCAGTC
ATCTGAGCCAGTTTATCTTCAACTATGCTCGTTTTGCTAAATTGCCATTGCCGCAAAAGTTGCCTGTCGATTGGCATTCA
CTCATTCAGCAACTAAAACAGCATTATCGGTTTGAGCTCGTGGGTGAGCTACCTCAAAATCAGGTTAGTCTGGACTTGAT
TCAGATGGAGCAAGTACTGCTTAATCTGCTGAAAAACGCCCATGAATCGGGCTCAGATTTGACAAAGATCACCATAGAGA
TCAGAGAGGTAAATCAAGTGGACCCCAGCAGCGGGATCTATATTGAAGTAAAAGATGAAGGTCTAGGAATGTCGGCAGAT
GTACTGCAACAAGCGTTATTGCCTTTTTACTCAACAAAGCAATCCGGTACAGGCTTAGGCTTGCCTCTTTGTCGAGAGAT
AGTTGAGGCGCACGATGGACGAATTAGCCTACATCATCGGGATGATAAAGGTCTATGTGTGAGCCTCTGGTTACCCTATG
CATAG

Upstream 100 bases:

>100_bases
CCAGCATAGAGGCGTGATAGCTCGTGTCGCTAAGGCTCTGGGCTTAAGTCGACAAGCCTTATATCGGCGCATGGACAAGT
ATGGGATTGATAAGTAATTC

Downstream 100 bases:

>100_bases
ACAGAGCTACGATAGAGAGAGCATGCCCCCTCTACTAAACTGATAAATTCAAGTTTTGAATCTAGCAAGAGTTAGCTAAA
GCGAGGATTTTTAATTTAGA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSNTVVSLRVKLIISAVLASATGLLLAFTGLLWLSPVAVQQNVVEQSWLYLLLFTLSLLLVTSISYIGTRDLATSLRALE
VGLLNFKDNDFSVSIPVSGDSQLRALSTLFNESAQTLRKERQYIYQRELLLDKVIQSSPNVMLLLDDEQRVIYANDAARH
LFNSGQRVEGMSLSQLLTDAPESLSEALNNPKDGLFSLKHDAGESDLETWHLSRGQFLLNGQFHHLVLLKQLTRELNRQE
VAVWKKVIRIISHELNNSVAPIASMVNSGRMITKELNDPKLKLIFDTIEDRSSHLSQFIFNYARFAKLPLPQKLPVDWHS
LIQQLKQHYRFELVGELPQNQVSLDLIQMEQVLLNLLKNAHESGSDLTKITIEIREVNQVDPSSGIYIEVKDEGLGMSAD
VLQQALLPFYSTKQSGTGLGLPLCREIVEAHDGRISLHHRDDKGLCVSLWLPYA

Sequences:

>Translated_454_residues
MSNTVVSLRVKLIISAVLASATGLLLAFTGLLWLSPVAVQQNVVEQSWLYLLLFTLSLLLVTSISYIGTRDLATSLRALE
VGLLNFKDNDFSVSIPVSGDSQLRALSTLFNESAQTLRKERQYIYQRELLLDKVIQSSPNVMLLLDDEQRVIYANDAARH
LFNSGQRVEGMSLSQLLTDAPESLSEALNNPKDGLFSLKHDAGESDLETWHLSRGQFLLNGQFHHLVLLKQLTRELNRQE
VAVWKKVIRIISHELNNSVAPIASMVNSGRMITKELNDPKLKLIFDTIEDRSSHLSQFIFNYARFAKLPLPQKLPVDWHS
LIQQLKQHYRFELVGELPQNQVSLDLIQMEQVLLNLLKNAHESGSDLTKITIEIREVNQVDPSSGIYIEVKDEGLGMSAD
VLQQALLPFYSTKQSGTGLGLPLCREIVEAHDGRISLHHRDDKGLCVSLWLPYA
>Mature_453_residues
SNTVVSLRVKLIISAVLASATGLLLAFTGLLWLSPVAVQQNVVEQSWLYLLLFTLSLLLVTSISYIGTRDLATSLRALEV
GLLNFKDNDFSVSIPVSGDSQLRALSTLFNESAQTLRKERQYIYQRELLLDKVIQSSPNVMLLLDDEQRVIYANDAARHL
FNSGQRVEGMSLSQLLTDAPESLSEALNNPKDGLFSLKHDAGESDLETWHLSRGQFLLNGQFHHLVLLKQLTRELNRQEV
AVWKKVIRIISHELNNSVAPIASMVNSGRMITKELNDPKLKLIFDTIEDRSSHLSQFIFNYARFAKLPLPQKLPVDWHSL
IQQLKQHYRFELVGELPQNQVSLDLIQMEQVLLNLLKNAHESGSDLTKITIEIREVNQVDPSSGIYIEVKDEGLGMSADV
LQQALLPFYSTKQSGTGLGLPLCREIVEAHDGRISLHHRDDKGLCVSLWLPYA

Specific function: Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals [H]

COG id: COG5000

COG function: function code T; Signal transduction histidine kinase involved in nitrogen fixation and metabolism regulation

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=123, Percent_Identity=37.3983739837398, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790300, Length=335, Percent_Identity=25.3731343283582, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788549, Length=375, Percent_Identity=24.5333333333333, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF08448 PAS_4 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51066; Mature: 50935

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTVVSLRVKLIISAVLASATGLLLAFTGLLWLSPVAVQQNVVEQSWLYLLLFTLSLLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VTSISYIGTRDLATSLRALEVGLLNFKDNDFSVSIPVSGDSQLRALSTLFNESAQTLRKE
HHHHHHHCHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RQYIYQRELLLDKVIQSSPNVMLLLDDEQRVIYANDAARHLFNSGQRVEGMSLSQLLTDA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECHHHHHHHCCCCCCCCCCHHHHHHCC
PESLSEALNNPKDGLFSLKHDAGESDLETWHLSRGQFLLNGQFHHLVLLKQLTRELNRQE
HHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
VAVWKKVIRIISHELNNSVAPIASMVNSGRMITKELNDPKLKLIFDTIEDRSSHLSQFIF
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHH
NYARFAKLPLPQKLPVDWHSLIQQLKQHYRFELVGELPQNQVSLDLIQMEQVLLNLLKNA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
HESGSDLTKITIEIREVNQVDPSSGIYIEVKDEGLGMSADVLQQALLPFYSTKQSGTGLG
CCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
LPLCREIVEAHDGRISLHHRDDKGLCVSLWLPYA
HHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCC
>Mature Secondary Structure 
SNTVVSLRVKLIISAVLASATGLLLAFTGLLWLSPVAVQQNVVEQSWLYLLLFTLSLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VTSISYIGTRDLATSLRALEVGLLNFKDNDFSVSIPVSGDSQLRALSTLFNESAQTLRKE
HHHHHHHCHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RQYIYQRELLLDKVIQSSPNVMLLLDDEQRVIYANDAARHLFNSGQRVEGMSLSQLLTDA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECHHHHHHHCCCCCCCCCCHHHHHHCC
PESLSEALNNPKDGLFSLKHDAGESDLETWHLSRGQFLLNGQFHHLVLLKQLTRELNRQE
HHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
VAVWKKVIRIISHELNNSVAPIASMVNSGRMITKELNDPKLKLIFDTIEDRSSHLSQFIF
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHH
NYARFAKLPLPQKLPVDWHSLIQQLKQHYRFELVGELPQNQVSLDLIQMEQVLLNLLKNA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
HESGSDLTKITIEIREVNQVDPSSGIYIEVKDEGLGMSADVLQQALLPFYSTKQSGTGLG
CCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
LPLCREIVEAHDGRISLHHRDDKGLCVSLWLPYA
HHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9218420 [H]