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The map label for this gene is rsuA [H]

Identifier: 157963031

GI number: 157963031

Start: 3940661

End: 3941380

Strand: Reverse

Name: rsuA [H]

Synonym: Spea_3214

Alternate gene names: 157963031

Gene position: 3941380-3940661 (Counterclockwise)

Preceding gene: 157963034

Following gene: 157963028

Centisome position: 76.17

GC content: 48.75

Gene sequence:

>720_bases
ATGCGTTTAGATAAATTTATCTGTAAGAGTACCGAGCTTAATCGCAAGCACGCTAGCGATTGTATCGAGCGCGCGGAAGT
GAGCGTTAACGGCGAGGTCATTACTGATGTGCGCTTTCAGGTACATGAAAGTAACGAGGTGCGCTATAAGGGGCAAAGGT
TAGTTGCGAGGCCGTCGCGCTACATCATGCTGCATAAGCCGATACATACCTTAAGCTCAAATGTCGATGGTGATTACCCA
AGCCTATTTCGCTGTATCGATATTGACAGGGTTGAGGATTTACATATTGTGGGTAGGCTCGATGCCGACACCACGGGCTT
AGTGTTACTGACCGATGATGGTCGCTGGTCATTCGATATTATTCGCCCCGACAAACAGTGTGCAAAAACCTACCGGGTGA
TGCTCAGAGATCCCATTGCTGGAGATGTAGCAGCCGATGTTATCGCAAGGTTTGAGGCTGGCCTACAACTGCAAAATGAG
CAGGCGTTAACCCTACCTGCGAAACTTGAGATTGTTGCGCCTAGAGAGGTGTTACTCACCATCACCGAGGGCAAGTATCA
CCAGGTAAAGCGTATGTTTGCCGCGGTGGGCAATCGCGTTATCGGTTTACACAGAGAACAAATTGGTGATTTAAGCTTAG
ATTTAAAGGTCGGTGAGTGGCGTTATCTTTCTGATACCGAGGTGAAGCGATTGTCGACTCCTACGCCTAGCGGTGACTGA

Upstream 100 bases:

>100_bases
TCTATTAGAGCGGATAATATAGGGCTTTTTAAAATGAGGGCTTGTGCAATTTTTACCGCTTGCGATAATTCCGCCATATA
AGAAGTGATAACAGAGTGAC

Downstream 100 bases:

>100_bases
CTGCATTCCCGCTAAAAATATAAAAGCCCTTATATCGATATCGATTAAGGGCTTTTTAGTTAGTTCATTATCTTGGCTAA
GGCTCCATTAGCGGGCTGTG

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRLDKFICKSTELNRKHASDCIERAEVSVNGEVITDVRFQVHESNEVRYKGQRLVARPSRYIMLHKPIHTLSSNVDGDYP
SLFRCIDIDRVEDLHIVGRLDADTTGLVLLTDDGRWSFDIIRPDKQCAKTYRVMLRDPIAGDVAADVIARFEAGLQLQNE
QALTLPAKLEIVAPREVLLTITEGKYHQVKRMFAAVGNRVIGLHREQIGDLSLDLKVGEWRYLSDTEVKRLSTPTPSGD

Sequences:

>Translated_239_residues
MRLDKFICKSTELNRKHASDCIERAEVSVNGEVITDVRFQVHESNEVRYKGQRLVARPSRYIMLHKPIHTLSSNVDGDYP
SLFRCIDIDRVEDLHIVGRLDADTTGLVLLTDDGRWSFDIIRPDKQCAKTYRVMLRDPIAGDVAADVIARFEAGLQLQNE
QALTLPAKLEIVAPREVLLTITEGKYHQVKRMFAAVGNRVIGLHREQIGDLSLDLKVGEWRYLSDTEVKRLSTPTPSGD
>Mature_239_residues
MRLDKFICKSTELNRKHASDCIERAEVSVNGEVITDVRFQVHESNEVRYKGQRLVARPSRYIMLHKPIHTLSSNVDGDYP
SLFRCIDIDRVEDLHIVGRLDADTTGLVLLTDDGRWSFDIIRPDKQCAKTYRVMLRDPIAGDVAADVIARFEAGLQLQNE
QALTLPAKLEIVAPREVLLTITEGKYHQVKRMFAAVGNRVIGLHREQIGDLSLDLKVGEWRYLSDTEVKRLSTPTPSGD

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=46.7532467532467, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1790453, Length=232, Percent_Identity=29.7413793103448, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87081838, Length=213, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787524, Length=233, Percent_Identity=27.4678111587983, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27094; Mature: 27094

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFICKSTELNRKHASDCIERAEVSVNGEVITDVRFQVHESNEVRYKGQRLVARPSR
CCCHHHHHCCCCCCHHHHHHHHHHHEEEECCEEEEEEEEEEECCCCEEECCCEEEECCCE
YIMLHKPIHTLSSNVDGDYPSLFRCIDIDRVEDLHIVGRLDADTTGLVLLTDDGRWSFDI
EEEEECCHHHHHCCCCCCCCHHEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEE
IRPDKQCAKTYRVMLRDPIAGDVAADVIARFEAGLQLQNEQALTLPAKLEIVAPREVLLT
ECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCEEECCEEEEEECCCEEEEE
ITEGKYHQVKRMFAAVGNRVIGLHREQIGDLSLDLKVGEWRYLSDTEVKRLSTPTPSGD
EECCHHHHHHHHHHHHCCEEEEEEHHHCCCEEEEEEECCEEECCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MRLDKFICKSTELNRKHASDCIERAEVSVNGEVITDVRFQVHESNEVRYKGQRLVARPSR
CCCHHHHHCCCCCCHHHHHHHHHHHEEEECCEEEEEEEEEEECCCCEEECCCEEEECCCE
YIMLHKPIHTLSSNVDGDYPSLFRCIDIDRVEDLHIVGRLDADTTGLVLLTDDGRWSFDI
EEEEECCHHHHHCCCCCCCCHHEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEE
IRPDKQCAKTYRVMLRDPIAGDVAADVIARFEAGLQLQNEQALTLPAKLEIVAPREVLLT
ECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCEEECCEEEEEECCCEEEEE
ITEGKYHQVKRMFAAVGNRVIGLHREQIGDLSLDLKVGEWRYLSDTEVKRLSTPTPSGD
EECCHHHHHHHHHHHHCCEEEEEEHHHCCCEEEEEEECCEEECCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]