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The map label for this gene is btuB [H]

Identifier: 157963023

GI number: 157963023

Start: 3930329

End: 3932182

Strand: Reverse

Name: btuB [H]

Synonym: Spea_3206

Alternate gene names: 157963023

Gene position: 3932182-3930329 (Counterclockwise)

Preceding gene: 157963024

Following gene: 157963022

Centisome position: 75.99

GC content: 45.63

Gene sequence:

>1854_bases
ATGGGAACACAACCCTCTAAAATTGCACTGCTAATTAGCAGTCTTATCAGCTTTACTGCATTCACAGCGACCGCCGAAGA
TCTCGGAGCAAGCTCAGCGACAATCGATGAAACGATTACTGTAACAGGATCACGTTTTGACAGAACGGTAGATCAGCAAC
TGACAGTTATCAATACGATTGAGCGTGAAGAAATCGCGCGTCTTAACCCTAAATCGGTTGCCGATGTACTAGAAACGCTT
CCTGGCGTCAGTGTATCTCGCAATGGTGGTGCGGGTCAGGCTACTTCTATTAGTGTTCGCGGTAGTAATTCAAATCATGT
TCTAGTGCTAGTTGACGGCGTAAAGGTTGGCTCTGCAACCTTAGGTACTGTCAGTTTTAATACCCTATCTCCTGAGAACA
TCGAGCGTATAGAAGTGCTTAAAGGTCCGCGGGCGTCTGTATGGGGCAGCGATGCTATAGGCGGCGTTATTCAAATATTT
ACCCGTGAGCTTAAAGGTGGCGAGTGGTTTGCTGGTGCCGAATATGGCAGCAATGACTATGTTCGAGGCTCATTTGGCGC
AGGCATGAATCATGGCGACGGAAACACCACACTTGCCGTTAACCATGAACAGTCTGACGGCTATGATGTATATAATGGCG
CCGAAGTCGAGAACGATGACGACGGTTACAAGCGTAATGCGCTTTCATTGAAAGGGTCGCAGCAAATTAATCAAAACTGG
CAAGCACTTTGGAATGGCCAGTACGATAAGGGCAATACCCAATACGATGACATCTACGCCTCAGGTTCGGCCGATGAGTC
AGATTATGAAAACTATCTTTGGAGTCTTGCTACCCAGTACAGTCGTGAGCAGTTCACTAGCAAGTTAGCCGTGAGTCAAT
CACAAGATTCAAATGAAAATTTCCGCGGTGATGATGCGAGTGTTGCCATTGCAGAGTTTGAAACTAAGCGCGATCAAGTT
AACTGGAGCAATCAGTATTTAGCGGCAGATGATCTGACTATTACCGGCGGTATTGACTGGTCAAGAGAGCAGGTCAGAGG
CGACTACGCCCAAGACGAGCGAGACCTTTTCGGCGCATACGCATTAATCCAGAAGCAATGGAGCAAGTTGCTAGCCGAAG
TGGCTGTACGCTACGATGATGTGGAAAATATCGATAGCGAAACTTCCTATAATGCCAGTTTAGCCTATCAGTTTAATGAA
CAATGGCGAATAGCAGCGACCACGGGCACGGCTTTTAAGGCGCCAACCTTTAACGATCTATACTGGCCTGACTCTGGTAA
CTCTGACTTGATCTCAGAAACGGCAGAGAGTTATGAGCTGACGCTGCATTTTAATAATCAAGATCTTCGCGCCTATATCA
GTGTGTTCCAGAATAAGATTGATAACCTTATTGCCTGGGCACCAACTGGTGAGCAAGATGATTTTGGTTGGGATATCTGG
AAGCCTGCGAACATCAATGAAGCTAAGATCACAGGTGTAGAGCTTTCGGCAAATTATCAACTGTTAGGGCTAGAACATCA
GCTGGGTTACACCTACCTAAATGCAGAAAACAAGCAAACTGATGAACAACTTATCGGTCGCAGTGAGAATGAGTTCAACT
ATTTACTGAGCTACACTTGGCAGCAGTTTGATCTACTTGCTAACTATCATTATCAAGGTAAGCGTTATGCTGGCTATGAG
CAGTACTTAGATGCCTATCATAAGCTCGATTTGAGTTTGGGTTTCCAGATTGATGATGCATGGAGCCTACGCTTAAAGGC
GAACAACCTGTTTGATGAAGAGATTATCTCAGATCAGAACTACTTTAGCCCTGGAAGAGAGTTATTCTTCAGCGTTAGCT
ACCAAGCGTTTTAA

Upstream 100 bases:

>100_bases
ATGCGGCAATTAACGAGCAAAACAAACAATAGCACTTGCCATTTATTGCTCGTATCACCGTGACGTTTTTGCCCCGTTTT
CCTTTAGGAGTCAAAGGTAA

Downstream 100 bases:

>100_bases
TTACCTATAGCTAATACTATTGTTATAGGAAGAAAGCCAGACATTGTGTCTGGCTTTTTTATGTTATAAATTGGCAAACT
CATAAGTAGGCGTACCAATG

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MGTQPSKIALLISSLISFTAFTATAEDLGASSATIDETITVTGSRFDRTVDQQLTVINTIEREEIARLNPKSVADVLETL
PGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSATLGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIF
TRELKGGEWFAGAEYGSNDYVRGSFGAGMNHGDGNTTLAVNHEQSDGYDVYNGAEVENDDDGYKRNALSLKGSQQINQNW
QALWNGQYDKGNTQYDDIYASGSADESDYENYLWSLATQYSREQFTSKLAVSQSQDSNENFRGDDASVAIAEFETKRDQV
NWSNQYLAADDLTITGGIDWSREQVRGDYAQDERDLFGAYALIQKQWSKLLAEVAVRYDDVENIDSETSYNASLAYQFNE
QWRIAATTGTAFKAPTFNDLYWPDSGNSDLISETAESYELTLHFNNQDLRAYISVFQNKIDNLIAWAPTGEQDDFGWDIW
KPANINEAKITGVELSANYQLLGLEHQLGYTYLNAENKQTDEQLIGRSENEFNYLLSYTWQQFDLLANYHYQGKRYAGYE
QYLDAYHKLDLSLGFQIDDAWSLRLKANNLFDEEIISDQNYFSPGRELFFSVSYQAF

Sequences:

>Translated_617_residues
MGTQPSKIALLISSLISFTAFTATAEDLGASSATIDETITVTGSRFDRTVDQQLTVINTIEREEIARLNPKSVADVLETL
PGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSATLGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIF
TRELKGGEWFAGAEYGSNDYVRGSFGAGMNHGDGNTTLAVNHEQSDGYDVYNGAEVENDDDGYKRNALSLKGSQQINQNW
QALWNGQYDKGNTQYDDIYASGSADESDYENYLWSLATQYSREQFTSKLAVSQSQDSNENFRGDDASVAIAEFETKRDQV
NWSNQYLAADDLTITGGIDWSREQVRGDYAQDERDLFGAYALIQKQWSKLLAEVAVRYDDVENIDSETSYNASLAYQFNE
QWRIAATTGTAFKAPTFNDLYWPDSGNSDLISETAESYELTLHFNNQDLRAYISVFQNKIDNLIAWAPTGEQDDFGWDIW
KPANINEAKITGVELSANYQLLGLEHQLGYTYLNAENKQTDEQLIGRSENEFNYLLSYTWQQFDLLANYHYQGKRYAGYE
QYLDAYHKLDLSLGFQIDDAWSLRLKANNLFDEEIISDQNYFSPGRELFFSVSYQAF
>Mature_616_residues
GTQPSKIALLISSLISFTAFTATAEDLGASSATIDETITVTGSRFDRTVDQQLTVINTIEREEIARLNPKSVADVLETLP
GVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSATLGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIFT
RELKGGEWFAGAEYGSNDYVRGSFGAGMNHGDGNTTLAVNHEQSDGYDVYNGAEVENDDDGYKRNALSLKGSQQINQNWQ
ALWNGQYDKGNTQYDDIYASGSADESDYENYLWSLATQYSREQFTSKLAVSQSQDSNENFRGDDASVAIAEFETKRDQVN
WSNQYLAADDLTITGGIDWSREQVRGDYAQDERDLFGAYALIQKQWSKLLAEVAVRYDDVENIDSETSYNASLAYQFNEQ
WRIAATTGTAFKAPTFNDLYWPDSGNSDLISETAESYELTLHFNNQDLRAYISVFQNKIDNLIAWAPTGEQDDFGWDIWK
PANINEAKITGVELSANYQLLGLEHQLGYTYLNAENKQTDEQLIGRSENEFNYLLSYTWQQFDLLANYHYQGKRYAGYEQ
YLDAYHKLDLSLGFQIDDAWSLRLKANNLFDEEIISDQNYFSPGRELFFSVSYQAF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=632, Percent_Identity=30.8544303797468, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1788478, Length=720, Percent_Identity=23.4722222222222, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786798, Length=188, Percent_Identity=33.5106382978723, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68929; Mature: 68797

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQPSKIALLISSLISFTAFTATAEDLGASSATIDETITVTGSRFDRTVDQQLTVINTI
CCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EREEIARLNPKSVADVLETLPGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSAT
HHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCEECCEE
LGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIFTRELKGGEWFAGAEYGSNDY
EEEEEECCCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
VRGSFGAGMNHGDGNTTLAVNHEQSDGYDVYNGAEVENDDDGYKRNALSLKGSQQINQNW
EEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHCCCH
QALWNGQYDKGNTQYDDIYASGSADESDYENYLWSLATQYSREQFTSKLAVSQSQDSNEN
HHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FRGDDASVAIAEFETKRDQVNWSNQYLAADDLTITGGIDWSREQVRGDYAQDERDLFGAY
CCCCCCEEEEEEECCCCCCCCCCCCEEEECCEEEECCCCCCHHHHCCCCCCCHHHHHHHH
ALIQKQWSKLLAEVAVRYDDVENIDSETSYNASLAYQFNEQWRIAATTGTAFKAPTFNDL
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEECCCEEEEEECCCEEECCCCCCC
YWPDSGNSDLISETAESYELTLHFNNQDLRAYISVFQNKIDNLIAWAPTGEQDDFGWDIW
CCCCCCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCC
KPANINEAKITGVELSANYQLLGLEHQLGYTYLNAENKQTDEQLIGRSENEFNYLLSYTW
CCCCCCCEEEEEEEEECCEEEEEEEHHCCEEEECCCCCCCHHHHHCCCCCCCEEEEEHHH
QQFDLLANYHYQGKRYAGYEQYLDAYHKLDLSLGFQIDDAWSLRLKANNLFDEEIISDQN
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECEEECCCEEEEEECCCCCHHHHHCCCC
YFSPGRELFFSVSYQAF
CCCCCCEEEEEEEEECC
>Mature Secondary Structure 
GTQPSKIALLISSLISFTAFTATAEDLGASSATIDETITVTGSRFDRTVDQQLTVINTI
CCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EREEIARLNPKSVADVLETLPGVSVSRNGGAGQATSISVRGSNSNHVLVLVDGVKVGSAT
HHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEECCEECCEE
LGTVSFNTLSPENIERIEVLKGPRASVWGSDAIGGVIQIFTRELKGGEWFAGAEYGSNDY
EEEEEECCCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
VRGSFGAGMNHGDGNTTLAVNHEQSDGYDVYNGAEVENDDDGYKRNALSLKGSQQINQNW
EEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHCCCH
QALWNGQYDKGNTQYDDIYASGSADESDYENYLWSLATQYSREQFTSKLAVSQSQDSNEN
HHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FRGDDASVAIAEFETKRDQVNWSNQYLAADDLTITGGIDWSREQVRGDYAQDERDLFGAY
CCCCCCEEEEEEECCCCCCCCCCCCEEEECCEEEECCCCCCHHHHCCCCCCCHHHHHHHH
ALIQKQWSKLLAEVAVRYDDVENIDSETSYNASLAYQFNEQWRIAATTGTAFKAPTFNDL
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEECCCEEEEEECCCEEECCCCCCC
YWPDSGNSDLISETAESYELTLHFNNQDLRAYISVFQNKIDNLIAWAPTGEQDDFGWDIW
CCCCCCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCC
KPANINEAKITGVELSANYQLLGLEHQLGYTYLNAENKQTDEQLIGRSENEFNYLLSYTW
CCCCCCCEEEEEEEEECCEEEEEEEHHCCEEEECCCCCCCHHHHHCCCCCCCEEEEEHHH
QQFDLLANYHYQGKRYAGYEQYLDAYHKLDLSLGFQIDDAWSLRLKANNLFDEEIISDQN
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECEEECCCEEEEEECCCCCHHHHHCCCC
YFSPGRELFFSVSYQAF
CCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA