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The map label for this gene is rrmJ [H]

Identifier: 157962886

GI number: 157962886

Start: 3764031

End: 3764660

Strand: Reverse

Name: rrmJ [H]

Synonym: Spea_3068

Alternate gene names: 157962886

Gene position: 3764660-3764031 (Counterclockwise)

Preceding gene: 157962888

Following gene: 157962885

Centisome position: 72.75

GC content: 47.3

Gene sequence:

>630_bases
ATGTCAGGAAAAAAACGAACGGCCAGTTCTTCTCGCTGGATGCAGGAACATTTCGACGATCACTATGTCAAACTGTCGCA
AAAGCGCGGTTTAAGGTCGCGTGCGGCCTTTAAGATTGAAGAGATCCAAGAAAAAGATAAACTCATTCGTCCGGGAATGA
CTGTTGTCGATTTAGGTGCCGCGCCGGGTGGCTGGTCTCAAATTGCCGTTAAGTTGGCAGGAGACAAGGGTAAAGTTATC
GCTTGTGATATTTTACCTATGGACCCGATTGTTGGTGTTGATTTCCTACAGGGCGATTTCCGCGAAGAGAAAGTACTGGA
CGCATTGTTAACCCGAGTGGGTGATGCCAAGGTCGATGTGGTGCTGTCAGATATGGCACCTAATATGAGTGGAACAGGTG
GTGTTGATCAGCCTCGAGCCATGTATTTAGTAGAACTAGCCTTAGATATGTGTCATCAAGTTTTGGCACCTAATGGCAGT
TTTGCGGTTAAAGTCTTTCAGGGGGAGGGCTTTGATGAATACATGAAAGCGGTTAAAGAGGCGTTCAAAACCGTTAAAAC
ACGTAAGCCCGATTCTTCACGGCCGCGATCGCGTGAAGTCTATCTTGTGGCGACGGGCTACAAGTTATAG

Upstream 100 bases:

>100_bases
AGTTCATTTATACCAACTTTATGCTGAGTTACTGTTGAAAACCCGCTATTCTACCCTTATATAGGCTTTGTGTAACTCTC
AATTTTGTTGCGGATTTTAA

Downstream 100 bases:

>100_bases
TAATCTATCGTAAGTACTCTAACATTAGTTTTCGGAAGACTTTAAAAGAGGTCGAGTAATTTGAGTGACATGGCAAAAAA
TTTAATTCTCTGGGTTGTCA

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSGKKRTASSSRWMQEHFDDHYVKLSQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGAAPGGWSQIAVKLAGDKGKVI
ACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDVVLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGS
FAVKVFQGEGFDEYMKAVKEAFKTVKTRKPDSSRPRSREVYLVATGYKL

Sequences:

>Translated_209_residues
MSGKKRTASSSRWMQEHFDDHYVKLSQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGAAPGGWSQIAVKLAGDKGKVI
ACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDVVLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGS
FAVKVFQGEGFDEYMKAVKEAFKTVKTRKPDSSRPRSREVYLVATGYKL
>Mature_208_residues
SGKKRTASSSRWMQEHFDDHYVKLSQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGAAPGGWSQIAVKLAGDKGKVIA
CDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDVVLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGSF
AVKVFQGEGFDEYMKAVKEAFKTVKTRKPDSSRPRSREVYLVATGYKL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=213, Percent_Identity=34.7417840375587, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=32.9787234042553, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=33.1578947368421, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=33.1578947368421, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=33.1578947368421, Blast_Score=108, Evalue=3e-24,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=74.0384615384615, Blast_Score=325, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=33.1818181818182, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=32.2751322751323, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=31.9371727748691, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=32.2751322751323, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=32.2751322751323, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=35.0785340314136, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=32.6633165829146, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=282, Percent_Identity=26.241134751773, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=35.6382978723404, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356387, Length=221, Percent_Identity=33.9366515837104, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648639, Length=198, Percent_Identity=32.8282828282828, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24647580, Length=200, Percent_Identity=31.5, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23096; Mature: 22965

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKKRTASSSRWMQEHFDDHYVKLSQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGA
CCCCCCCCHHHHHHHHHCCHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQIAVKLAGDKGKVIACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDV
CCCCHHEEEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEE
VLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGSFAVKVFQGEGFDEYMKAVKE
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFKTVKTRKPDSSRPRSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SGKKRTASSSRWMQEHFDDHYVKLSQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGA
CCCCCCCHHHHHHHHHCCHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQIAVKLAGDKGKVIACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDV
CCCCHHEEEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEE
VLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGSFAVKVFQGEGFDEYMKAVKE
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFKTVKTRKPDSSRPRSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA