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The map label for this gene is tpiA [H]

Identifier: 157962882

GI number: 157962882

Start: 3758701

End: 3759483

Strand: Reverse

Name: tpiA [H]

Synonym: Spea_3064

Alternate gene names: 157962882

Gene position: 3759483-3758701 (Counterclockwise)

Preceding gene: 157962883

Following gene: 157962881

Centisome position: 72.65

GC content: 44.57

Gene sequence:

>783_bases
ATGGCACTTAGACGTCCCATGGTTGCTGGTAATTGGAAAATGAACGGCAGTGCGCAGTTGGCGCAAGAGCTATTCAATAA
GTTCGCTACCAAGCTCCAAGATGATTCAGTGGAAGTGGTTTTGTGTCCACCTTCTATTTACTTAGAGAGTGTTCGACAAC
TACTAGATGAAAATAAAGAAACCTTAAATGGTTGCTTAGTCAGAATGGGAACTCAAAACCTAAGTCAACATGACTTTGGA
GCCTATACTGGTGAAATATCTGGTCAGATGTTAAAGGATTCAGGATGTCGATATGTTATTATCGGGCATTCAGAACGTCG
CCGTATGTACGGAGAGACGAGTAACATAGTGGCAGAGAAATTTGCTGCAGCTCAGAAACATGGTTTGACCCCAATATTAT
GTGTTGGTGAGTCGGGTCCAGCTCGTGAAGCGAGACGCACTTTTGAAGTGATCGCCGAAGAGTTAGATGTGGTCATTGAA
AAGAATGGCACCATGGCTTTTGACAACGCTATTATCGCTTACGAGCCATTATGGGCAGTAGGGACAGGTAAGAGTGCTAC
GCCAGAGCAGGCTCAAGAAGTTCATGCGTTTATCCGCAAACGCCTCTCTGAAGTATCTCCATTTATCGGAGAAAATATCA
GGATTTTGTACGGTGGTAGCGTAACACCGAGTAATGCAGCAGACTTGTTTGCTCAACCTGATGTAGACGGTGGATTGATT
GGCGGTGTAAGCCTCAATGCTACCGAATTTCTCAGTTTATGTACGATAGCGATGAGCGCATAA

Upstream 100 bases:

>100_bases
AATACGCGTTCTCTCCTGAATTCGTAAAAATAACGCTTTTAGCTATTGTAACTTAACAAGTGGTTCGCTATTATTCACGC
CGCTTTCAAAGGAGACTGTA

Downstream 100 bases:

>100_bases
TATGTATGAAGTTCTAATGGTTGTTTACTTGTTGGTGTCGATTGGTCTAGTAGGCTTGATCTTAATCCAGCAAGGTAAAG
GTGCTGACATGGGTGCTTCT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MALRRPMVAGNWKMNGSAQLAQELFNKFATKLQDDSVEVVLCPPSIYLESVRQLLDENKETLNGCLVRMGTQNLSQHDFG
AYTGEISGQMLKDSGCRYVIIGHSERRRMYGETSNIVAEKFAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIE
KNGTMAFDNAIIAYEPLWAVGTGKSATPEQAQEVHAFIRKRLSEVSPFIGENIRILYGGSVTPSNAADLFAQPDVDGGLI
GGVSLNATEFLSLCTIAMSA

Sequences:

>Translated_260_residues
MALRRPMVAGNWKMNGSAQLAQELFNKFATKLQDDSVEVVLCPPSIYLESVRQLLDENKETLNGCLVRMGTQNLSQHDFG
AYTGEISGQMLKDSGCRYVIIGHSERRRMYGETSNIVAEKFAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIE
KNGTMAFDNAIIAYEPLWAVGTGKSATPEQAQEVHAFIRKRLSEVSPFIGENIRILYGGSVTPSNAADLFAQPDVDGGLI
GGVSLNATEFLSLCTIAMSA
>Mature_259_residues
ALRRPMVAGNWKMNGSAQLAQELFNKFATKLQDDSVEVVLCPPSIYLESVRQLLDENKETLNGCLVRMGTQNLSQHDFGA
YTGEISGQMLKDSGCRYVIIGHSERRRMYGETSNIVAEKFAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIEK
NGTMAFDNAIIAYEPLWAVGTGKSATPEQAQEVHAFIRKRLSEVSPFIGENIRILYGGSVTPSNAADLFAQPDVDGGLIG
GVSLNATEFLSLCTIAMSA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=252, Percent_Identity=43.2539682539683, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI226529917, Length=252, Percent_Identity=43.2539682539683, Blast_Score=194, Evalue=5e-50,
Organism=Escherichia coli, GI1790353, Length=259, Percent_Identity=46.7181467181467, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=43.8524590163934, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=251, Percent_Identity=40.6374501992032, Blast_Score=185, Evalue=7e-48,
Organism=Drosophila melanogaster, GI28572008, Length=251, Percent_Identity=44.2231075697211, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI28572006, Length=251, Percent_Identity=44.2231075697211, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI28572004, Length=251, Percent_Identity=44.2231075697211, Blast_Score=205, Evalue=2e-53,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 28276; Mature: 28144

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRRPMVAGNWKMNGSAQLAQELFNKFATKLQDDSVEVVLCPPSIYLESVRQLLDENKE
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCHH
TLNGCLVRMGTQNLSQHDFGAYTGEISGQMLKDSGCRYVIIGHSERRRMYGETSNIVAEK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHCCCHHHHHHHH
FAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIEKNGTMAFDNAIIAYEPLWAV
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCEEECCEEEEECCEEEE
GTGKSATPEQAQEVHAFIRKRLSEVSPFIGENIRILYGGSVTPSNAADLFAQPDVDGGLI
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCEEEEECCCCCCCCCHHCCCCCCCCCCEE
GGVSLNATEFLSLCTIAMSA
CCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALRRPMVAGNWKMNGSAQLAQELFNKFATKLQDDSVEVVLCPPSIYLESVRQLLDENKE
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCHH
TLNGCLVRMGTQNLSQHDFGAYTGEISGQMLKDSGCRYVIIGHSERRRMYGETSNIVAEK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHCCCHHHHHHHH
FAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIEKNGTMAFDNAIIAYEPLWAV
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCEEECCEEEEECCEEEE
GTGKSATPEQAQEVHAFIRKRLSEVSPFIGENIRILYGGSVTPSNAADLFAQPDVDGGLI
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCEEEEECCCCCCCCCHHCCCCCCCCCCEE
GGVSLNATEFLSLCTIAMSA
CCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA