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The map label for this gene is pnp [H]

Identifier: 157962873

GI number: 157962873

Start: 3746741

End: 3748855

Strand: Reverse

Name: pnp [H]

Synonym: Spea_3055

Alternate gene names: 157962873

Gene position: 3748855-3746741 (Counterclockwise)

Preceding gene: 157962875

Following gene: 157962872

Centisome position: 72.45

GC content: 44.87

Gene sequence:

>2115_bases
ATGGTTCACGTGAATCCAATCGTAAAGAGTTTTCAATATGGTCAGCACACAGTCACATTAGAGACTGGGGTTATTGCACG
TCAAGCAGATGCAGCTGTTTTGGCAAGTATGGGTGATACAACAGTATTGGTTACTGTTGTTGGCAAGAAGTTTGAAGAGC
CAGGCCGTGACTTTTTCCCGCTAACCGTTAATTACCAGGAAAAAACATACGCAGCAGGTAAGATCCCTGGTGGATTCTTT
AAGCGTGAAGGTCGTCCATCTGAAGGCGAAACGCTTACAGCTCGTCTAATTGACCGTCCAATCCGTCCACTATTCCCGAA
TGGTTTCAAGAACGAAGTTCAGGTTATCATTACAGTAGTTTCTGTTGATCCAGAAATCAGCCCAGAAGTTATCTCTATGA
TCGGTACTTCAGCTGCACTTTCTATCTCTGGTATTCCATTTAATGGTCCACTAGGTTCAGCGCGTGTTGGTTATGTTAAT
GGTGAATATATCCTTAACCCAACAGTTTCTCAGCTAGTTGATAGCCAGCTAGAGCTTTCTGTTGCTGGTACTGAAAGTGC
AGTACTAATGGTTGAATCAGAAGCAAGCGCACTACCTGAAGAAGTGATGTTAGGTGCGGTTGTTTATGGTCACGATCAGC
AGCAAGTGGTTATTCAAGCAATTAAAGAGCTACAAGCTGAAGTTAACAAGCCTGTATGGGATTGGTCTGCACCTGCGCAA
GACGAAACGCTTGTTGCTAAGATCAAAGATTTGGCTGAAGCAGGTCTTACTGAAGCTTACCAAATTGAAGTTAAGCAAGA
TCGTTACGCTCAAGTTGGTGTAGTTAAGAATGCTGCTAAAGAAGCATTATTGGCTGAAAACCCAGATGCTAACACACGTG
AAATCGACGGTCTTCTAGGTAGCCTAGAGAAGAAAGTGGTTCGTGGCCGTATTATCTCTGGTCAGCCACGTATCGATGGT
CGTGAGCCAGATATGGTTCGTGCACTTAACGTTATGGCTGGTGTACTTCCACGTACTCACGGTAGCTCACTGTTCACTCG
TGGTGAAACTCAGGCGCTAGTAACTTGTACTCTTGGTACAGAGCGTGATGCACAGAAGATTGATAGCATCATGGGCGAGT
ACACTAACCGCTTTATGCTTCACTACAACTTCCCTCCATACTCTGTTGGTGAAACAGGCATGGTTGGTTCTCCTAAGCGT
CGTGAAATTGGTCACGGTAAGCTAGCATGGCGTGGTATTAACGCGGTTATGCCTACTGCTGAAGAGTTCCCATACAGCGT
TCGTATCGTATCTGAAATCACTGAATCTAACGGTTCAAGCTCTATGGCGTCAGTTTGTGGTACTTCTCTTGCTCTTATGG
ATGCAGGTGTACCAATCAAGACTTCTGTTGCGGGTATCGCGATGGGTCTAGTTAAAGAAGGCGACGATTTCGTTGTTCTT
TCTGACATCCTAGGCGATGAAGATCACCTTGGTGACATGGACTTTAAAGTTGCGGGTACTCGCGACGGTATTACTGCTCT
GCAGATGGATATCAAGATCGAAGGTATCACTAAAGAGATCATGCAGATTGCTCTTAAGCAAGCATACGGCGCTCGTGTTC
ATATCCTAGACGTAATGGATAGGGCTATCTCTGGCCACCGTGGTGATATTTCTGAGCATGCTCCACGTATCACAACTATC
AAGATCAACCCTGAGAAGATCCGTGATGTTATTGGTAAAGGTGGTGCAACAATTCGTGCACTTACTGAAGAAACCGGTAC
GACTATCGAACTTGATGACGACGGTACAGTGAAGATTGCTTCTTCAAATGGCGAAGCGACTAAAGAAGCTATCCGTCGTA
TCGAAGAGATCACTGCAGAAGTTGAAGTCGGTACTGTATACAACGGTAAAGTTGTTCGTATCGTAGATTTTGGTGCATTC
GTAACAATTCTTCCTGGTAAAGATGGTCTAGTACACATTTCTCAAATCGCTGAAGAGCGTGTTGCGAACGTGTCTGACTA
CTTAGAAGTAGGTCAAGAAGTTAAAGTTAAAGTGATGGAAGTTGACCGTCAAGGCCGTGTACGTCTTTCTATGAAAGAAG
CTGCACCTAAAGCCGAAGCGCCAGCTGCTGAATAA

Upstream 100 bases:

>100_bases
AGGGAAAAGATTCCGCTTTGTCATCAATCTATTCTCTTTATCTATTGAATAAACTTGCAGTATACTTACGCGCGTAATTA
AGGTAATTAAATATTAAGGA

Downstream 100 bases:

>100_bases
GCCCTAGCACTTTGAATTAAAAGGAGGCCATAGGCCTCCTTTTTTATGCCTGCAATAAAGTCAGCTGCTGAATTAATCAA
CGATAAGTGGATTATTTTCA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MVHVNPIVKSFQYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVVSVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVN
GEYILNPTVSQLVDSQLELSVAGTESAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWSAPAQ
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLGSLEKKVVRGRIISGQPRIDG
REPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGTERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPTAEEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALKQAYGARVHILDVMDRAISGHRGDISEHAPRITTI
KINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIASSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAF
VTILPGKDGLVHISQIAEERVANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKAEAPAAE

Sequences:

>Translated_704_residues
MVHVNPIVKSFQYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVVSVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVN
GEYILNPTVSQLVDSQLELSVAGTESAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWSAPAQ
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLGSLEKKVVRGRIISGQPRIDG
REPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGTERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPTAEEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALKQAYGARVHILDVMDRAISGHRGDISEHAPRITTI
KINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIASSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAF
VTILPGKDGLVHISQIAEERVANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKAEAPAAE
>Mature_704_residues
MVHVNPIVKSFQYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVVSVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVN
GEYILNPTVSQLVDSQLELSVAGTESAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWSAPAQ
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLGSLEKKVVRGRIISGQPRIDG
REPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGTERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPTAEEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALKQAYGARVHILDVMDRAISGHRGDISEHAPRITTI
KINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIASSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAF
VTILPGKDGLVHISQIAEERVANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKAEAPAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=707, Percent_Identity=38.048090523338, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=72.2701149425287, Blast_Score=1043, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=709, Percent_Identity=32.4400564174894, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17535281, Length=75, Percent_Identity=49.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281362905, Length=708, Percent_Identity=37.8531073446328, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=708, Percent_Identity=37.8531073446328, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=708, Percent_Identity=37.8531073446328, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=654, Percent_Identity=37.4617737003058, Blast_Score=417, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75917; Mature: 75917

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHVNPIVKSFQYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFP
CEECCHHHHHHHCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCHHCCCCCCEEE
LTVNYQEKTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVV
EEEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCEEEEEEEE
SVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVNGEYILNPTVSQLVDSQLELS
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCCHHHHCCCCEEEE
VAGTESAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWSAPAQ
EECCCEEEEEEECCHHHCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLG
CCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SLEKKVVRGRIISGQPRIDGREPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGT
HHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCC
ERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPTA
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
EEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
HHCCEEEEEEHHHHCCCCCCHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALKQAYGARVHILDVMD
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHH
RAISGHRGDISEHAPRITTIKINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIA
HHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
SSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAFVTILPGKDGLVHISQIAEER
CCCCHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEECCEEEEEECCCCCCEEHHHHHHHH
VANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKAEAPAAE
HHCHHHHHHCCCCEEEEEEEECCCCCEEEEHHHCCCCCCCCCCC
>Mature Secondary Structure
MVHVNPIVKSFQYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFP
CEECCHHHHHHHCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCHHCCCCCCEEE
LTVNYQEKTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVV
EEEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCEEEEEEEE
SVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVNGEYILNPTVSQLVDSQLELS
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCCHHHHCCCCEEEE
VAGTESAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWSAPAQ
EECCCEEEEEEECCHHHCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLG
CCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SLEKKVVRGRIISGQPRIDGREPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGT
HHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCC
ERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPTA
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
EEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
HHCCEEEEEEHHHHCCCCCCHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALKQAYGARVHILDVMD
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHH
RAISGHRGDISEHAPRITTIKINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIA
HHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
SSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAFVTILPGKDGLVHISQIAEER
CCCCHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEECCEEEEEECCCCCCEEHHHHHHHH
VANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKAEAPAAE
HHCHHHHHHCCCCEEEEEEEECCCCCEEEEHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA