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The map label for this gene is gadA [H]

Identifier: 157962771

GI number: 157962771

Start: 3615440

End: 3616834

Strand: Reverse

Name: gadA [H]

Synonym: Spea_2953

Alternate gene names: 157962771

Gene position: 3616834-3615440 (Counterclockwise)

Preceding gene: 157962773

Following gene: 157962770

Centisome position: 69.9

GC content: 46.09

Gene sequence:

>1395_bases
ATGCCTCTTCACTCAAAAGATACTGTGCGAGATGACCTCTTAGATGATATCTACTCATCGTCTGACTTAGCTTTATCCAT
GCCTAAATATAAGATGCCTGAGCAGGAAAATAATCCACGTCATGCATATCAAATCGTACACGACGAGCTGATGATGGACG
GAAACTCCCGTCAAAATCTAGCGACATTTTGCCAAACTTGGGTTGAGGATGAAGTTCATCAATTGATGGACGAATGTATC
GATAAGAATATGATCGATAAAGATGAGTACCCTCAAACTGCAGAGCTTGAAGCCCGTTGCGTGCATATGCTTGCCGACCT
TTGGAACTCTCCCGACGCTGAAAATACCTTAGGCTGCTCAACCACTGGCTCGAGTGAGGCTGCGATGCTCGGTGGTATGG
CATTGAAATGGGCTTGGCGTAAGAAGATGAAAGCGTTAGGTAAACCAACGGATAAACCCAATATGGTTTGTGGACCGGTG
CAAGTTTGCTGGCATAAATTCGCGCGCTATTGGGATATAGAGTTACGTGAGATCCCGATGGAAGGCGATCGTTTAATCAT
GAATGCTGAAGAAGTGATTAAGCGCTGCGATGAAAATACCATAGGTGTTGTACCGACTTTAGGGGTGACGTTCACCTGTC
AGTACGAGCCTGTAAAGGCGGTACATGATGCGCTCGATAAGTTGCAAAAAGAGACTGGACTGGATATTCCAATGCATGTT
GATGCCGCCAGTGGAGGCTTCTTAGCGCCATTTTGCCAACCAGAACTAGAATGGGACTTTAAATTACCAAGGGTAAAATC
AATTAATGCGTCTGGGCATAAATTTGGCTTGAGCCCTCTTGGTGTGGGTTGGGTTATTTGGCGCGATGCGTCGGCCTTAG
ATGAAGACCTTATTTTTAATGTGAACTACCTTGGCGGTAACATGCCGACCTTTGCGCTGAACTTCTCTCGACCTGGTGGA
CAGATAGTTGCGCAATACTACAACTTTTTGCGCTTAGGTAAAGAAGGCTACAGAAAAATCCATCAGGCTTGTTACGACAC
CGCGCAATACCTGTCGAGTGAGATTGAGAAACTGGGGATGTTCGAGATCATTTATGATGGTCATGACGGGATCCCTGCGA
TGAGTTGGTCGTTAAAAGAGGGCGTTGACCCAGGGTTTAACTTGTTTGATCTCTCTGATCGTATCCGTTCTCGAGGCTGG
CAGATTGCGGCCTATGCCATGCCGCCGAAGAGAGAGGATTTAGTCATTATGCGTATTCTGGTACGGCATGGGTTTAGCCG
AGATCAAGCGGACTTATTAGTAGCTGACTTGAAGCACTGTGTTGAATTTTTTGCCGAACACCCAATTTCACATGGTAGTG
ATGCCAAAGAGTCGTCTGGCTTTAATCATGGTTAA

Upstream 100 bases:

>100_bases
TGGGTGTTGCGGCGATGGGCGCTATCTTCTATGTTGGTTACGTCAGCTGACCATCGCCAGCTAGTAGGAAATTTTATTGA
ATTCATTGGAGTAAAATATA

Downstream 100 bases:

>100_bases
TTAGTCGAGAGGCCGTCCATAAAGACGGCCTTTTTCTATATAACTTAGTGAGAAATGATTATGAATATTTCTGAGTTATT
CAGTTTTGTGTTTAGTTATA

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPLHSKDTVRDDLLDDIYSSSDLALSMPKYKMPEQENNPRHAYQIVHDELMMDGNSRQNLATFCQTWVEDEVHQLMDECI
DKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMVCGPV
QVCWHKFARYWDIELREIPMEGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDKLQKETGLDIPMHV
DAASGGFLAPFCQPELEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASALDEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGMFEIIYDGHDGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVEFFAEHPISHGSDAKESSGFNHG

Sequences:

>Translated_464_residues
MPLHSKDTVRDDLLDDIYSSSDLALSMPKYKMPEQENNPRHAYQIVHDELMMDGNSRQNLATFCQTWVEDEVHQLMDECI
DKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMVCGPV
QVCWHKFARYWDIELREIPMEGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDKLQKETGLDIPMHV
DAASGGFLAPFCQPELEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASALDEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGMFEIIYDGHDGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVEFFAEHPISHGSDAKESSGFNHG
>Mature_463_residues
PLHSKDTVRDDLLDDIYSSSDLALSMPKYKMPEQENNPRHAYQIVHDELMMDGNSRQNLATFCQTWVEDEVHQLMDECID
KNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMVCGPVQ
VCWHKFARYWDIELREIPMEGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDKLQKETGLDIPMHVD
AASGGFLAPFCQPELEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASALDEDLIFNVNYLGGNMPTFALNFSRPGGQ
IVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGMFEIIYDGHDGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQ
IAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVEFFAEHPISHGSDAKESSGFNHG

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=340, Percent_Identity=26.4705882352941, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1787769, Length=459, Percent_Identity=57.0806100217865, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789934, Length=458, Percent_Identity=57.2052401746725, Blast_Score=566, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI17557272, Length=374, Percent_Identity=25.668449197861, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17543922, Length=331, Percent_Identity=24.1691842900302, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25148342, Length=321, Percent_Identity=22.4299065420561, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323905, Length=457, Percent_Identity=39.6061269146608, Blast_Score=317, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6320500, Length=340, Percent_Identity=25, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355963, Length=376, Percent_Identity=26.063829787234, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654344, Length=376, Percent_Identity=26.063829787234, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52490; Mature: 52359

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHSKDTVRDDLLDDIYSSSDLALSMPKYKMPEQENNPRHAYQIVHDELMMDGNSRQNL
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
ATFCQTWVEDEVHQLMDECIDKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMVCGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDKLQKETGLDIPMHV
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEE
DAASGGFLAPFCQPELEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASALDEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCCCCCCCCCEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FEIIYDGHDGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEEECCCCCCCCCCCHHHCCCCCCCEECHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCVEFFAEHPISHGSDAKESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
PLHSKDTVRDDLLDDIYSSSDLALSMPKYKMPEQENNPRHAYQIVHDELMMDGNSRQNL
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
ATFCQTWVEDEVHQLMDECIDKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWRKKMKALGKPTDKPNMVCGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMNAEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHDALDKLQKETGLDIPMHV
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEE
DAASGGFLAPFCQPELEWDFKLPRVKSINASGHKFGLSPLGVGWVIWRDASALDEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCCCCCCCCCEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAQYLSSEIEKLGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FEIIYDGHDGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEEECCCCCCCCCCCHHHCCCCCCCEECHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCVEFFAEHPISHGSDAKESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]