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The map label for this gene is gltS [H]

Identifier: 157962766

GI number: 157962766

Start: 3607988

End: 3609217

Strand: Reverse

Name: gltS [H]

Synonym: Spea_2948

Alternate gene names: 157962766

Gene position: 3609217-3607988 (Counterclockwise)

Preceding gene: 157962768

Following gene: 157962765

Centisome position: 69.75

GC content: 45.04

Gene sequence:

>1230_bases
ATGGAACCACAGTTAATTGAAATAAAAGACTTTGTCTCTTTTACCTTAGCGATAATAGCGCTATTTATCGGTAAATATAT
TATCTCTCGATACGAGTTACTCAGAAAGTACAGTATCCCAGAGCCTGTTGTTGGCGGCTTTGCTTGCGCCGCGATTGTAG
GCATTCTTTATTATGCCTTCGATATTCAAATCGAGTTTAACCTCGAGATCCGCGATGTGCTATTGCTCTATTTCTTTGCA
GGTATTGGCTTGAAAGCCGACTTTGCCACCCTATTAAAGGGCGGAAAGCCGCTACTTATCTTAATTGCCCTAGCGAGTGT
GTTCATCTTCTTGCAAAACTTTACCGGCGTCACCATTGCCACCTTGTTTGGTTTAGATCCCAAGGCTGGCTTGATGTCTG
GCTCCATTTCACTCATCGGTGGGGTTGGCACTGCCATGGCGTGGACCCCTACTTTTGTTGAAGAATTGGGTATCGTCAAC
GCCAGTGAGTTGGGTATAGCATCTAACACCCTAGGACTTATTTCTGCCTGTGTGATTGGTGGCCCTATTGCGGCTTACTT
GATGAAACGTCATAAGGTGCAAGCTAATCATGACTCAGAGCTGGATATTGGTACTAGCCATCAGGCGGGCTCATCACATA
TTAAGGTCGACTACTTTGGCGTATTACGAGCTTGGTTATGGTTAAACGTCACCCTGATTATTGGCTACTTTATCGATCTG
GCCCTACAAGAGGCAGGGGTTAAACTGCCTATGTTCGTCGCCTGCCTATTAGCCGGTATTATTATCGGTAATGTAGGAAG
AGCCATCATCAATCGCAGAGAGGTCAAAGATAGAGCCTACTTAGATGATGCCTCACAGGGCTTGTCTCTTATTCAAGATA
TCTGTTTGGGTATGTTCCTGACCATGGCTTTGATGAGTCTTAAGGTGTGGGAATTAGAGGGCAGCTTACTCTATATATCG
GTGGTCATGTCGGTGCAGGTATTCTTGTCGGTGGTATTTACCATTTTTGTGGTATTTAGAATGATGGGCAAAGACTATGA
GGCGGCAGTGATTTGCTCAGGTTTTGGTGGCGTGACCTTAGGTTCGACGGCAACGGCGATTGTGAATATGACAGCTGTGA
CGCAGCAGTACGGTGCGGCTCACAGGGCCTTTATTATCGTGCCGCTAGTCTGTGGCTTCTTTATCGATATTATCAATGCG
ATGATCATTAATTTATTCGTCTATTTTTAA

Upstream 100 bases:

>100_bases
ACCCCCAGCGGGCTTCAGCTTGTGCGGGCTTGGTCGTTAGCGAGGAGGGGCATTTCAGCTGTTTCTTATTTTATAGATAT
AAAGGGATTGAACGATTGTC

Downstream 100 bases:

>100_bases
GATTTAATCTTTCAGACTTAGCGGTAATCGTGGTTAGTAGAAGGGAGCAAATGCTCCCTTTTTACTTAGATGGAAAAGAA
ATAAATAAAAGGTAGTGAGC

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MEPQLIEIKDFVSFTLAIIALFIGKYIISRYELLRKYSIPEPVVGGFACAAIVGILYYAFDIQIEFNLEIRDVLLLYFFA
GIGLKADFATLLKGGKPLLILIALASVFIFLQNFTGVTIATLFGLDPKAGLMSGSISLIGGVGTAMAWTPTFVEELGIVN
ASELGIASNTLGLISACVIGGPIAAYLMKRHKVQANHDSELDIGTSHQAGSSHIKVDYFGVLRAWLWLNVTLIIGYFIDL
ALQEAGVKLPMFVACLLAGIIIGNVGRAIINRREVKDRAYLDDASQGLSLIQDICLGMFLTMALMSLKVWELEGSLLYIS
VVMSVQVFLSVVFTIFVVFRMMGKDYEAAVICSGFGGVTLGSTATAIVNMTAVTQQYGAAHRAFIIVPLVCGFFIDIINA
MIINLFVYF

Sequences:

>Translated_409_residues
MEPQLIEIKDFVSFTLAIIALFIGKYIISRYELLRKYSIPEPVVGGFACAAIVGILYYAFDIQIEFNLEIRDVLLLYFFA
GIGLKADFATLLKGGKPLLILIALASVFIFLQNFTGVTIATLFGLDPKAGLMSGSISLIGGVGTAMAWTPTFVEELGIVN
ASELGIASNTLGLISACVIGGPIAAYLMKRHKVQANHDSELDIGTSHQAGSSHIKVDYFGVLRAWLWLNVTLIIGYFIDL
ALQEAGVKLPMFVACLLAGIIIGNVGRAIINRREVKDRAYLDDASQGLSLIQDICLGMFLTMALMSLKVWELEGSLLYIS
VVMSVQVFLSVVFTIFVVFRMMGKDYEAAVICSGFGGVTLGSTATAIVNMTAVTQQYGAAHRAFIIVPLVCGFFIDIINA
MIINLFVYF
>Mature_409_residues
MEPQLIEIKDFVSFTLAIIALFIGKYIISRYELLRKYSIPEPVVGGFACAAIVGILYYAFDIQIEFNLEIRDVLLLYFFA
GIGLKADFATLLKGGKPLLILIALASVFIFLQNFTGVTIATLFGLDPKAGLMSGSISLIGGVGTAMAWTPTFVEELGIVN
ASELGIASNTLGLISACVIGGPIAAYLMKRHKVQANHDSELDIGTSHQAGSSHIKVDYFGVLRAWLWLNVTLIIGYFIDL
ALQEAGVKLPMFVACLLAGIIIGNVGRAIINRREVKDRAYLDDASQGLSLIQDICLGMFLTMALMSLKVWELEGSLLYIS
VVMSVQVFLSVVFTIFVVFRMMGKDYEAAVICSGFGGVTLGSTATAIVNMTAVTQQYGAAHRAFIIVPLVCGFFIDIINA
MIINLFVYF

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=384, Percent_Identity=36.9791666666667, Blast_Score=238, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 44399; Mature: 44399

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPQLIEIKDFVSFTLAIIALFIGKYIISRYELLRKYSIPEPVVGGFACAAIVGILYYAF
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHE
DIQIEFNLEIRDVLLLYFFAGIGLKADFATLLKGGKPLLILIALASVFIFLQNFTGVTIA
EEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHH
TLFGLDPKAGLMSGSISLIGGVGTAMAWTPTFVEELGIVNASELGIASNTLGLISACVIG
HHHCCCCCCCCCCCCHHHCCCCCHHHHCCHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHH
GPIAAYLMKRHKVQANHDSELDIGTSHQAGSSHIKVDYFGVLRAWLWLNVTLIIGYFIDL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
ALQEAGVKLPMFVACLLAGIIIGNVGRAIINRREVKDRAYLDDASQGLSLIQDICLGMFL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TMALMSLKVWELEGSLLYISVVMSVQVFLSVVFTIFVVFRMMGKDYEAAVICSGFGGVTL
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
GSTATAIVNMTAVTQQYGAAHRAFIIVPLVCGFFIDIINAMIINLFVYF
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEPQLIEIKDFVSFTLAIIALFIGKYIISRYELLRKYSIPEPVVGGFACAAIVGILYYAF
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHE
DIQIEFNLEIRDVLLLYFFAGIGLKADFATLLKGGKPLLILIALASVFIFLQNFTGVTIA
EEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHH
TLFGLDPKAGLMSGSISLIGGVGTAMAWTPTFVEELGIVNASELGIASNTLGLISACVIG
HHHCCCCCCCCCCCCHHHCCCCCHHHHCCHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHH
GPIAAYLMKRHKVQANHDSELDIGTSHQAGSSHIKVDYFGVLRAWLWLNVTLIIGYFIDL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
ALQEAGVKLPMFVACLLAGIIIGNVGRAIINRREVKDRAYLDDASQGLSLIQDICLGMFL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TMALMSLKVWELEGSLLYISVVMSVQVFLSVVFTIFVVFRMMGKDYEAAVICSGFGGVTL
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
GSTATAIVNMTAVTQQYGAAHRAFIIVPLVCGFFIDIINAMIINLFVYF
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]