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The map label for this gene is nitA [H]

Identifier: 157962682

GI number: 157962682

Start: 3495733

End: 3496677

Strand: Reverse

Name: nitA [H]

Synonym: Spea_2863

Alternate gene names: 157962682

Gene position: 3496677-3495733 (Counterclockwise)

Preceding gene: 157962685

Following gene: 157962674

Centisome position: 67.57

GC content: 47.51

Gene sequence:

>945_bases
ATGAAAAAAGTCGCAATTATTCAAGAGGCGCCCTGTGTGCTAGATAAGAAAGCAACGATTCAAAAAGCCGTAGAAATTGT
TCATTCGGTATCAGAAAAAGGTGCTGAACTTATTGTTTTCCCTGAGGCATTTATTCCAGGATACCCAGCCTGGATATGGC
GCTTAAGACCTGGGGCGGACTGGGGCGTGAGTGAAGCCTTACATACGCGTTTGCTACAAAACTCGGTGGATATGTCATCG
GATGATCTTAAGCCGCTATTAGAGGCGGCCAAACAGAGAGCTGTGACTCTAGTTTGTGGGTTAAACGAGCGAGATAATGC
TAATAGCCAGTCGACCATCTATAACACCGCCATCACTATCGATCCCCAAGGGAAAATCGTTAACCATCACCGTAAGCTTA
TGCCGACGAATCCGGAGCGTATGGTTTGGGGCTTTGGTGATGGCCATGGGCTAAACGTGATTGCTACGCCAGTAGGAAAA
ATAGCGAGCCTCATATGCTGGGAAAATTATATGCCACTGGCGAGGTACGCCCTATATTCACAGGGGGTAGAAATATATAT
CGCACCAACCTATGACAGCGGTGAAGCTTGGATTGGCACTATGCAGCATATTGCTAGAGAGGGAAAGTGTTGGGTACTGT
GCTGTGGTGTCGCGCTGCAAAGAAAAGATCTACCAGAAGACTTTCCCGATCTCGATAGACTCTACCCGGCAGATGAGGAT
TGGATTAATCCCGGAGACTCGCTCATCGTCTCCCCAAATGGCGAAATCATGGCAGGCCCCATGTCTAAAGAGAAGGGCTA
TTTACTGTTAGATATTGATGTGGCAAAGGCGGCTAGCTCAAAGCGGGCGCTGGATGTTGCAGGGCATTATTCTAGGCCGG
ATGTGTTTAAATTAGAAGTCGATAAGTCTCGTCAGTCACCAGTCAGCTTTAAGAATCAGAGCTAA

Upstream 100 bases:

>100_bases
ATATGCTGGCTAGCCTCGAGTTAAAACTTTCTGGCGCCATCCTTTTAATACGTCAATGAACACGCCATTAACTAAACCGT
TCATTACAAGGGAGCAAATC

Downstream 100 bases:

>100_bases
CTAAGCGAAACTTACTCAGCAGTGTTAACTTTTGCGTGAGAGCACCTTTTATACCAGTTTCCCATTAGTGAACTAGGTGT
TAGATTAAGGCAGGATGTAG

Product: Nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGADWGVSEALHTRLLQNSVDMSS
DDLKPLLEAAKQRAVTLVCGLNERDNANSQSTIYNTAITIDPQGKIVNHHRKLMPTNPERMVWGFGDGHGLNVIATPVGK
IASLICWENYMPLARYALYSQGVEIYIAPTYDSGEAWIGTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
WINPGDSLIVSPNGEIMAGPMSKEKGYLLLDIDVAKAASSKRALDVAGHYSRPDVFKLEVDKSRQSPVSFKNQS

Sequences:

>Translated_314_residues
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGADWGVSEALHTRLLQNSVDMSS
DDLKPLLEAAKQRAVTLVCGLNERDNANSQSTIYNTAITIDPQGKIVNHHRKLMPTNPERMVWGFGDGHGLNVIATPVGK
IASLICWENYMPLARYALYSQGVEIYIAPTYDSGEAWIGTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
WINPGDSLIVSPNGEIMAGPMSKEKGYLLLDIDVAKAASSKRALDVAGHYSRPDVFKLEVDKSRQSPVSFKNQS
>Mature_314_residues
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGADWGVSEALHTRLLQNSVDMSS
DDLKPLLEAAKQRAVTLVCGLNERDNANSQSTIYNTAITIDPQGKIVNHHRKLMPTNPERMVWGFGDGHGLNVIATPVGK
IASLICWENYMPLARYALYSQGVEIYIAPTYDSGEAWIGTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
WINPGDSLIVSPNGEIMAGPMSKEKGYLLLDIDVAKAASSKRALDVAGHYSRPDVFKLEVDKSRQSPVSFKNQS

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=316, Percent_Identity=39.2405063291139, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322027, Length=202, Percent_Identity=38.1188118811881, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 34787; Mature: 34787

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGAD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEEECCCCC
WGVSEALHTRLLQNSVDMSSDDLKPLLEAAKQRAVTLVCGLNERDNANSQSTIYNTAITI
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEEEEEE
DPQGKIVNHHRKLMPTNPERMVWGFGDGHGLNVIATPVGKIASLICWENYMPLARYALYS
CCCCCEECCCHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHH
QGVEIYIAPTYDSGEAWIGTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
CCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCHHHCCCCCCC
WINPGDSLIVSPNGEIMAGPMSKEKGYLLLDIDVAKAASSKRALDVAGHYSRPDVFKLEV
CCCCCCEEEECCCCCEEECCCCCCCCEEEEEEHHHHCCCCCCEEEECCCCCCCCEEEEEE
DKSRQSPVSFKNQS
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGAD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEEECCCCC
WGVSEALHTRLLQNSVDMSSDDLKPLLEAAKQRAVTLVCGLNERDNANSQSTIYNTAITI
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEEEEEE
DPQGKIVNHHRKLMPTNPERMVWGFGDGHGLNVIATPVGKIASLICWENYMPLARYALYS
CCCCCEECCCHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHH
QGVEIYIAPTYDSGEAWIGTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
CCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCHHHCCCCCCC
WINPGDSLIVSPNGEIMAGPMSKEKGYLLLDIDVAKAASSKRALDVAGHYSRPDVFKLEV
CCCCCCEEEECCCCCEEECCCCCCCCEEEEEEHHHHCCCCCCEEEECCCCCCCCEEEEEE
DKSRQSPVSFKNQS
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]