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The map label for this gene is hemN [H]

Identifier: 157962215

GI number: 157962215

Start: 2918541

End: 2919827

Strand: Reverse

Name: hemN [H]

Synonym: Spea_2394

Alternate gene names: 157962215

Gene position: 2919827-2918541 (Counterclockwise)

Preceding gene: 157962216

Following gene: 157962213

Centisome position: 56.43

GC content: 41.72

Gene sequence:

>1287_bases
ATGTCATTTGCCGATCTACGTTTAAGTTTAGCAACACAAAGCCTGGATTTTGTTATGCAGCATACAATTACGCATTCGCT
TAGCTTAGCGGATGCTACAGTGCTTCCAGCCTCGCTGTCATCAGTAGCTTTTACCCAAGAGCAACATATTGAAACCCTTT
ATATTCATATTCCTTTTTGCCATACACTTTGCCGCTTCTGCTCATTTCATAAAATTAAATATAACCAACAAAAAGCGCTC
GAATATTTCCAGGCTTTACGAAAAGAAATACGCCACGTGATTGAGAGCGGTCTTCGTTTTAACCGGGTATACATTGGTGG
CGGTACAACGACGATACTAGAAGATGAGTTAGTTAAGACCATCGAGTTAATTAAAAGCTTAACCACAGTGCGTGAAATTT
CTTGTGAGACTGACCCTAGCTACTTTAGAACGGGTCATCCTGAAGTACTTAATGGCTTAGTAGATCGAATGTCTATTGGT
TTACAGAGCTTTGACGATAGAATATTAAAAGCCAGTAGCCGGTTTGAAAAGTTCGGTTCAGGTGATGAACAAGAAATTGA
TATTAGACGCGGCATCGAACTGTTTCCTACGGTTAATGTGGACTTAATGTACGGGTTTAAAGGCCAGACTCCCGATAGTG
TCGTGACGGATATTAAGCGGTTGATCCAATTGGAACCCGACCAAATAACCACCTACCCGTTGACGCTAGGGATAGGTAAG
AGTCGTAAAAAGTCTCAATGTTTAGCGGGCGAGCCTAAAGATCTTTTTCCACAGTTTTTGGCCGTTAAGCAGGTGATGTC
AGATCGCTATATGATGGAGTTCCCATGGACTTACAGCCGTAATTTCGGCCAAGCTGTTGAGAACAAATACGTGTTAGATG
GTGAAAACTGCTTTGGTGTGGGATCGGGTGCGTTTGGTCGCTTTGGCGAGCAATTTCAGATATCAAGCTTTAGTATTGAT
AAATATATTCAATTGATTAATCAAGGCGGCGTAGGTACAAGTCATCATAAATTACTTAGCGTAAAATCGATAAATCAGCA
TCATTTAATGATAATGATGGGGAAAGGCTGCTTAGATAACGCGCTGTTTAAAGCCCATACGGGTAAGAGCTTGTGGCAAG
CCTTTCCTGTCGAACTCACTTACCTATTAGCTTCTGGCGCGCTAATTAAGCAAGGGGATAGCTATTTTACCACCGAGGCT
GGCCAATTTATTGCGCTAAAAATGTTTAGTGGTTTTTTAGCGGGAATGGATTATTTACGTGAGCAAGCAATTGAGTTGGC
CGCTTAA

Upstream 100 bases:

>100_bases
ACATCGGCTTTGTAACACACTTTTTAATGTAATCAAGGAGTAAGGTATTAGGTAATCACTTACCTGAGGTTTAGGTAAAC
TCAGCATCACAAGGTGAGTT

Downstream 100 bases:

>100_bases
TGTTTGCTAGCCGTTAAGCTCTTAGTTGTTTTAGTACTTAGTTGTTTTAGAGCTTAGTTTTAGTACTGGATTAGTTGAAA
GCTGATTACGAATGAAAAAT

Product: coproporphyrinogen dehydrogenase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSFADLRLSLATQSLDFVMQHTITHSLSLADATVLPASLSSVAFTQEQHIETLYIHIPFCHTLCRFCSFHKIKYNQQKAL
EYFQALRKEIRHVIESGLRFNRVYIGGGTTTILEDELVKTIELIKSLTTVREISCETDPSYFRTGHPEVLNGLVDRMSIG
LQSFDDRILKASSRFEKFGSGDEQEIDIRRGIELFPTVNVDLMYGFKGQTPDSVVTDIKRLIQLEPDQITTYPLTLGIGK
SRKKSQCLAGEPKDLFPQFLAVKQVMSDRYMMEFPWTYSRNFGQAVENKYVLDGENCFGVGSGAFGRFGEQFQISSFSID
KYIQLINQGGVGTSHHKLLSVKSINQHHLMIMMGKGCLDNALFKAHTGKSLWQAFPVELTYLLASGALIKQGDSYFTTEA
GQFIALKMFSGFLAGMDYLREQAIELAA

Sequences:

>Translated_428_residues
MSFADLRLSLATQSLDFVMQHTITHSLSLADATVLPASLSSVAFTQEQHIETLYIHIPFCHTLCRFCSFHKIKYNQQKAL
EYFQALRKEIRHVIESGLRFNRVYIGGGTTTILEDELVKTIELIKSLTTVREISCETDPSYFRTGHPEVLNGLVDRMSIG
LQSFDDRILKASSRFEKFGSGDEQEIDIRRGIELFPTVNVDLMYGFKGQTPDSVVTDIKRLIQLEPDQITTYPLTLGIGK
SRKKSQCLAGEPKDLFPQFLAVKQVMSDRYMMEFPWTYSRNFGQAVENKYVLDGENCFGVGSGAFGRFGEQFQISSFSID
KYIQLINQGGVGTSHHKLLSVKSINQHHLMIMMGKGCLDNALFKAHTGKSLWQAFPVELTYLLASGALIKQGDSYFTTEA
GQFIALKMFSGFLAGMDYLREQAIELAA
>Mature_427_residues
SFADLRLSLATQSLDFVMQHTITHSLSLADATVLPASLSSVAFTQEQHIETLYIHIPFCHTLCRFCSFHKIKYNQQKALE
YFQALRKEIRHVIESGLRFNRVYIGGGTTTILEDELVKTIELIKSLTTVREISCETDPSYFRTGHPEVLNGLVDRMSIGL
QSFDDRILKASSRFEKFGSGDEQEIDIRRGIELFPTVNVDLMYGFKGQTPDSVVTDIKRLIQLEPDQITTYPLTLGIGKS
RKKSQCLAGEPKDLFPQFLAVKQVMSDRYMMEFPWTYSRNFGQAVENKYVLDGENCFGVGSGAFGRFGEQFQISSFSIDK
YIQLINQGGVGTSHHKLLSVKSINQHHLMIMMGKGCLDNALFKAHTGKSLWQAFPVELTYLLASGALIKQGDSYFTTEAG
QFIALKMFSGFLAGMDYLREQAIELAA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=303, Percent_Identity=24.7524752475248, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87082341, Length=210, Percent_Identity=34.2857142857143, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789325, Length=288, Percent_Identity=25.3472222222222, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 48182; Mature: 48051

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFADLRLSLATQSLDFVMQHTITHSLSLADATVLPASLSSVAFTQEQHIETLYIHIPFC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECHH
HTLCRFCSFHKIKYNQQKALEYFQALRKEIRHVIESGLRFNRVYIGGGTTTILEDELVKT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
IELIKSLTTVREISCETDPSYFRTGHPEVLNGLVDRMSIGLQSFDDRILKASSRFEKFGS
HHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDEQEIDIRRGIELFPTVNVDLMYGFKGQTPDSVVTDIKRLIQLEPDQITTYPLTLGIGK
CCCCCCHHHCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCC
SRKKSQCLAGEPKDLFPQFLAVKQVMSDRYMMEFPWTYSRNFGQAVENKYVLDGENCFGV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHCCCHHHHHCCCEEECCCCCEEC
GSGAFGRFGEQFQISSFSIDKYIQLINQGGVGTSHHKLLSVKSINQHHLMIMMGKGCLDN
CCCCHHCCCCEEEECCCCHHHHHHHHHCCCCCCCHHHEEEEECCCCCEEEEEECCCCHHH
ALFKAHTGKSLWQAFPVELTYLLASGALIKQGDSYFTTEAGQFIALKMFSGFLAGMDYLR
HHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCEEECCCCCEEHHHHHHHHHHHHHHHH
EQAIELAA
HHHHHHCC
>Mature Secondary Structure 
SFADLRLSLATQSLDFVMQHTITHSLSLADATVLPASLSSVAFTQEQHIETLYIHIPFC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECHH
HTLCRFCSFHKIKYNQQKALEYFQALRKEIRHVIESGLRFNRVYIGGGTTTILEDELVKT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
IELIKSLTTVREISCETDPSYFRTGHPEVLNGLVDRMSIGLQSFDDRILKASSRFEKFGS
HHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDEQEIDIRRGIELFPTVNVDLMYGFKGQTPDSVVTDIKRLIQLEPDQITTYPLTLGIGK
CCCCCCHHHCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCC
SRKKSQCLAGEPKDLFPQFLAVKQVMSDRYMMEFPWTYSRNFGQAVENKYVLDGENCFGV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHCCCHHHHHCCCEEECCCCCEEC
GSGAFGRFGEQFQISSFSIDKYIQLINQGGVGTSHHKLLSVKSINQHHLMIMMGKGCLDN
CCCCHHCCCCEEEECCCCHHHHHHHHHCCCCCCCHHHEEEEECCCCCEEEEEECCCCHHH
ALFKAHTGKSLWQAFPVELTYLLASGALIKQGDSYFTTEAGQFIALKMFSGFLAGMDYLR
HHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCEEECCCCCEEHHHHHHHHHHHHHHHH
EQAIELAA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]