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The map label for this gene is yafJ [H]

Identifier: 157962213

GI number: 157962213

Start: 2914909

End: 2915694

Strand: Reverse

Name: yafJ [H]

Synonym: Spea_2392

Alternate gene names: 157962213

Gene position: 2915694-2914909 (Counterclockwise)

Preceding gene: 157962215

Following gene: 157962210

Centisome position: 56.35

GC content: 44.27

Gene sequence:

>786_bases
ATGTGCGAGTTGCTGGCCATGAGCGCCAATGTACCGACTGATATAGTATTTAGTTTTACTGGATTAGCACAAAGGGGCGG
AGTCACTGGGCCTCATGTCGACGGTTGGGGGATCACTTTCTACGAAGGTAAGGGCAATCGCACTTTTAAGGATGCTCGTC
CTAGCAGCGAATCTCATATTGCCCAGTTAATTAAATCTTATCCGATAAAGAGCGAAGTGGTAGTAAGCCATATTCGTCAA
GCCAACCGTGGTTGTGTCTCTCTTGAGAATACTCATCCGTTTACACGGGAGCTTTGGGGGCAATATTGGACTTATGCCCA
CAATGGCCAGTTGAGTGATTATGAAGACAAGTTTGTGTCCAAGCGTTTTACTGCGGTTGGAGATACAGATAGTGAAATGG
CTTTTTGCTGGATCATGGATCAAGTGGTAGAGAAGTTTGGTGATCAAGCTCCCGATGATAGGTTAACTGTTTATCAATAC
ATATCGACCCTCGCAGATGAAATTCGCGCCTTAGGCGTGTTCAATATGATCTTAAGTGATGGTGAGCATTTAATGAGTTA
TTGTAGTAATAACCTTTGTTACATCACTCGCCGTGCTCCTTTCGGCAAGGCTAAATTGATTGATACCGATGTGGTAATCG
ATTTTGATAAAGAGACGACACCTAATGATGTGGTCACAGTGATTGCCACGCGACCGCTCACCAATAATGAACAGTGGAAT
GTGCTTACACCAGGTGAGTGGAAGCTGTTTAAAAAAGGTGAGTTGGTGAGTTCATCACAGAGCTAA

Upstream 100 bases:

>100_bases
ATGTTTAATTTATTTGTACGCAGTCAGCATTCCACAATGGCTATCAAGTGGTTAGAATGGCGTTATTAATGATGTAGTAG
ATTGAATGGGATAGAGATTA

Downstream 100 bases:

>100_bases
ATAATTGCTAGAAAATAAAAAGCTCGCAAGGTTAAACCTAGCGAGCTTTTTTATTGGCATGTTTATGGTTAGTAAAGTCA
GCCTTCCTCGTTGCTAGCCT

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHIAQLIKSYPIKSEVVVSHIRQ
ANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEDKFVSKRFTAVGDTDSEMAFCWIMDQVVEKFGDQAPDDRLTVYQY
ISTLADEIRALGVFNMILSDGEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
VLTPGEWKLFKKGELVSSSQS

Sequences:

>Translated_261_residues
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHIAQLIKSYPIKSEVVVSHIRQ
ANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEDKFVSKRFTAVGDTDSEMAFCWIMDQVVEKFGDQAPDDRLTVYQY
ISTLADEIRALGVFNMILSDGEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
VLTPGEWKLFKKGELVSSSQS
>Mature_261_residues
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHIAQLIKSYPIKSEVVVSHIRQ
ANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEDKFVSKRFTAVGDTDSEMAFCWIMDQVVEKFGDQAPDDRLTVYQY
ISTLADEIRALGVFNMILSDGEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
VLTPGEWKLFKKGELVSSSQS

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=256, Percent_Identity=65.625, Blast_Score=355, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29373; Mature: 29373

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHI
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
AQLIKSYPIKSEVVVSHIRQANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEDKFVS
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCEEEECCCCCCHHHHHHH
KRFTAVGDTDSEMAFCWIMDQVVEKFGDQAPDDRLTVYQYISTLADEIRALGVFNMILSD
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
HHHHHHHHCCCEEEEEECCCCCCCEEEECEEEEECCCCCCCCCEEEEEEECCCCCCCCEE
VLTPGEWKLFKKGELVSSSQS
EECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGKGNRTFKDARPSSESHI
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
AQLIKSYPIKSEVVVSHIRQANRGCVSLENTHPFTRELWGQYWTYAHNGQLSDYEDKFVS
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCEEEECCCCCCHHHHHHH
KRFTAVGDTDSEMAFCWIMDQVVEKFGDQAPDDRLTVYQYISTLADEIRALGVFNMILSD
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEHLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTPNDVVTVIATRPLTNNEQWN
HHHHHHHHCCCEEEEEECCCCCCCEEEECEEEEECCCCCCCCCEEEEEEECCCCCCCCEE
VLTPGEWKLFKKGELVSSSQS
EECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]