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The map label for this gene is gacS [H]

Identifier: 157962196

GI number: 157962196

Start: 2896373

End: 2898793

Strand: Reverse

Name: gacS [H]

Synonym: Spea_2375

Alternate gene names: 157962196

Gene position: 2898793-2896373 (Counterclockwise)

Preceding gene: 157962197

Following gene: 157962188

Centisome position: 56.02

GC content: 36.51

Gene sequence:

>2421_bases
ATGCTTAAACAGGGAAGTTGGTCCATAAAGACATTTTTGCTAGCAGGAAGTGTCGCAATTGTTATATTGCTCATTGTTAA
TGTATCTATAACTCAGTACGTGTCGAATACTAAGTTGGATTTATCAAGGCTAGAAAATGAGGTTGTTGTATCAGCCAATA
CATTACTGATGATACGGCGCAGCGAAAAGGATTTTATTAGTCGAATAGACCCGAAGTATACTTTTCAAGTACTGGATTTT
AAAGCGAAATTGGTACAGCAATTAACTCAGATTGATCGGTTGCTGACTCAAAGTAATTTAGATATAAGCTATAATAATCA
ACGAGTATTAGAAGAGCTTGATGATTATATAGACACTTTTTCTATGTTAATTAATGAAATATACAGCATCAATGGTACCA
GTGCTGAGCAAGGTTTACTCGCACGGTTTGAACACAACTCTAATGAGCTCCAATTACTCACTAATCAGTTAAAAGATACA
GAACTATTAGAACTTATTATTGCCAATCAGCTAACAGTATTTGATTTTTTTAAAAGTTTTGACTCCAGGTTACTCACAAG
GATTACTCATCAGTTTAAAATAATAGAACAGCACATTATAGATAAACCTCAATCTACATTACCGTTGCAACAAGCATTTT
TGTCATTTGAGCATGCTTTTTTTCAGTTACAGTCATCGATGAAAAGGAGAGGTTACTCGGAGTCGTTAGGCTATCATGGA
ACGCTCAGGCAAAATGTTCACAGTGTGGAACGTAGTCTAGAAACTTTGTTTAATACGATCCCGCTTAAAGTTGATAAAAG
TGTTTCAGGGTTAGTCGTTACCCAGTCTATAGTAGAGCTGCTATTAGTGCTCTTAATCATTGGGATCCTATATTATATTT
TTTATGCCATCACTAGGATGGAGAAAGGGCTTATTTTCGCAAGAGAAAAAGAGCAACAGGCGAACAGTGCTAAAAGCTCA
TTTCTGGCTAATATGTCTCATGAAATTAGAACGCCGCTCAATGGTATCATTGGTATGACAGAGATTCTAACGGACTCAAA
ACTTACAGCGATACAGAAAGATTACCTTGCAACAATTAACTCGTCTTCTCAAACCTTATTAATGTTAATAAATGATATAT
TAGATTTATCAAAAATTGAGTCTGGCCACCTTGAGTTTTGTCCGCATACTTGCAATATAAAAGAGGTTATATATGACACT
GCTGCTTTAATTACACCTAAGGCTCAGCAAAAGAATATAGTACTCAGAATTATAATCGGCGATGAAGTGCCAACATATAT
CTGCGCCGATGAACAAAAGCTGCGTCAGATTTTAATGAATTTAGCTTCGAATGCCATTAAGTTTACACGTTCAGGTTCGG
TGTCATTTAAATTAGTGGTAAAAGCGGAGGAAGGTGAGAATATTCGTTTGCTCTTTTCAGTTAAAGATACTGGAGTCGGT
ATAGATAAGAATAAGCATGCGAAAATATTCGAGGAGTTTCAACAAGAGGAAAGTAATACTTCCATTGATTATGGTGGAAC
GGGGTTGGGCCTCTCTATTGCCACCAAAATGGTAAAAATAATGGGGGGCGAAATAATGATTAGTTCTGATAAAGGTGTTG
GGAGTGAATTTCATTTTATCTTAGCCTTTCCAAAACAAGAAGAAAGAGTCTGCGAACGACTCGACTCACAACATTTGATT
TATTGTGCGATTAAGCATAGCCCTCTTCTAGTTCAAAATATTATTGATTCAAATCATCAACTCGATAAAATAACTAAAGT
GTCTGAAATCAGGCCGCTATTAAAGCCTTCAAGTGTAATCGTGTTAAATGTTGATGATATTTCCGATAAACAGGTACTGG
TTGATATTGCGAAAGAGTGTTCATTGACTCCAATTATCATTGCTAGAGCAAATAATTCGATTAATACTGATTTTGGTGAG
CTGGTCGCTGGATACATTACATTGCCATTGTTAGGTGAGCGTTTGAATACCTTAATTAAGTCTGTATCCCATTGTCAAAA
GCAAGAAGAGGATCTTGTCCCGTTAAACAGTGACTCCCAAAAGAGTAAGAAAATAGTGCTGGTAGTTGAAGATAATAAAG
TGAACCAACAGGTTGTCTCTATTAACTTAAAAAAGCTAAACCTTCCGTATCTTATCGCCAATGACGGCAGGGAAGCATTG
GAGAATTATAAACGTCATATCGGAGGCGTTTCTGTCATTTTAATGGATTGTATGATGCCAATCATGGATGGTTTTGAGGC
AACGAGAGCAATTCGTATTTTTGAAAAAGAAGAAGGCGAACCGCGAGTTACAATTATAGCCTTAACTGCATCAATTTTAG
ACGATGATATTCAAAAGTGTTTTGAGTCAGGGATGGATGATTACTTGCCAAAGCCTTTCAAGCGAGATGTATTGGTCCGC
AAACTTGCGAAGCAGCATTAA

Upstream 100 bases:

>100_bases
GTATTTTCCTTCATATTTTCATTCCATTTTACAAATTGATTATTGGTGCTAACTGTTTATATAGCCTATTGTTTATGGAC
TGTGTGTAACAGGGGGTATC

Downstream 100 bases:

>100_bases
CAATGATTATTTGTATCGCTATATTCACAATATTGCTTATAAAATGAATGGTTTAACATGCTTAATGAGACTAGATAGAG
CTCAGGTTTGAGCCCTATCT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MLKQGSWSIKTFLLAGSVAIVILLIVNVSITQYVSNTKLDLSRLENEVVVSANTLLMIRRSEKDFISRIDPKYTFQVLDF
KAKLVQQLTQIDRLLTQSNLDISYNNQRVLEELDDYIDTFSMLINEIYSINGTSAEQGLLARFEHNSNELQLLTNQLKDT
ELLELIIANQLTVFDFFKSFDSRLLTRITHQFKIIEQHIIDKPQSTLPLQQAFLSFEHAFFQLQSSMKRRGYSESLGYHG
TLRQNVHSVERSLETLFNTIPLKVDKSVSGLVVTQSIVELLLVLLIIGILYYIFYAITRMEKGLIFAREKEQQANSAKSS
FLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEFCPHTCNIKEVIYDT
AALITPKAQQKNIVLRIIIGDEVPTYICADEQKLRQILMNLASNAIKFTRSGSVSFKLVVKAEEGENIRLLFSVKDTGVG
IDKNKHAKIFEEFQQEESNTSIDYGGTGLGLSIATKMVKIMGGEIMISSDKGVGSEFHFILAFPKQEERVCERLDSQHLI
YCAIKHSPLLVQNIIDSNHQLDKITKVSEIRPLLKPSSVIVLNVDDISDKQVLVDIAKECSLTPIIIARANNSINTDFGE
LVAGYITLPLLGERLNTLIKSVSHCQKQEEDLVPLNSDSQKSKKIVLVVEDNKVNQQVVSINLKKLNLPYLIANDGREAL
ENYKRHIGGVSVILMDCMMPIMDGFEATRAIRIFEKEEGEPRVTIIALTASILDDDIQKCFESGMDDYLPKPFKRDVLVR
KLAKQH

Sequences:

>Translated_806_residues
MLKQGSWSIKTFLLAGSVAIVILLIVNVSITQYVSNTKLDLSRLENEVVVSANTLLMIRRSEKDFISRIDPKYTFQVLDF
KAKLVQQLTQIDRLLTQSNLDISYNNQRVLEELDDYIDTFSMLINEIYSINGTSAEQGLLARFEHNSNELQLLTNQLKDT
ELLELIIANQLTVFDFFKSFDSRLLTRITHQFKIIEQHIIDKPQSTLPLQQAFLSFEHAFFQLQSSMKRRGYSESLGYHG
TLRQNVHSVERSLETLFNTIPLKVDKSVSGLVVTQSIVELLLVLLIIGILYYIFYAITRMEKGLIFAREKEQQANSAKSS
FLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEFCPHTCNIKEVIYDT
AALITPKAQQKNIVLRIIIGDEVPTYICADEQKLRQILMNLASNAIKFTRSGSVSFKLVVKAEEGENIRLLFSVKDTGVG
IDKNKHAKIFEEFQQEESNTSIDYGGTGLGLSIATKMVKIMGGEIMISSDKGVGSEFHFILAFPKQEERVCERLDSQHLI
YCAIKHSPLLVQNIIDSNHQLDKITKVSEIRPLLKPSSVIVLNVDDISDKQVLVDIAKECSLTPIIIARANNSINTDFGE
LVAGYITLPLLGERLNTLIKSVSHCQKQEEDLVPLNSDSQKSKKIVLVVEDNKVNQQVVSINLKKLNLPYLIANDGREAL
ENYKRHIGGVSVILMDCMMPIMDGFEATRAIRIFEKEEGEPRVTIIALTASILDDDIQKCFESGMDDYLPKPFKRDVLVR
KLAKQH
>Mature_806_residues
MLKQGSWSIKTFLLAGSVAIVILLIVNVSITQYVSNTKLDLSRLENEVVVSANTLLMIRRSEKDFISRIDPKYTFQVLDF
KAKLVQQLTQIDRLLTQSNLDISYNNQRVLEELDDYIDTFSMLINEIYSINGTSAEQGLLARFEHNSNELQLLTNQLKDT
ELLELIIANQLTVFDFFKSFDSRLLTRITHQFKIIEQHIIDKPQSTLPLQQAFLSFEHAFFQLQSSMKRRGYSESLGYHG
TLRQNVHSVERSLETLFNTIPLKVDKSVSGLVVTQSIVELLLVLLIIGILYYIFYAITRMEKGLIFAREKEQQANSAKSS
FLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEFCPHTCNIKEVIYDT
AALITPKAQQKNIVLRIIIGDEVPTYICADEQKLRQILMNLASNAIKFTRSGSVSFKLVVKAEEGENIRLLFSVKDTGVG
IDKNKHAKIFEEFQQEESNTSIDYGGTGLGLSIATKMVKIMGGEIMISSDKGVGSEFHFILAFPKQEERVCERLDSQHLI
YCAIKHSPLLVQNIIDSNHQLDKITKVSEIRPLLKPSSVIVLNVDDISDKQVLVDIAKECSLTPIIIARANNSINTDFGE
LVAGYITLPLLGERLNTLIKSVSHCQKQEEDLVPLNSDSQKSKKIVLVVEDNKVNQQVVSINLKKLNLPYLIANDGREAL
ENYKRHIGGVSVILMDCMMPIMDGFEATRAIRIFEKEEGEPRVTIIALTASILDDDIQKCFESGMDDYLPKPFKRDVLVR
KLAKQH

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=511, Percent_Identity=30.1369863013699, Blast_Score=216, Evalue=5e-57,
Organism=Escherichia coli, GI145693157, Length=521, Percent_Identity=30.9021113243762, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI48994928, Length=394, Percent_Identity=30.2030456852792, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI87081816, Length=233, Percent_Identity=38.1974248927039, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1788713, Length=233, Percent_Identity=32.618025751073, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=29.4354838709677, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790436, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=306, Percent_Identity=27.1241830065359, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788393, Length=220, Percent_Identity=25.9090909090909, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=291, Percent_Identity=27.1477663230241, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=25.2212389380531, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=24.7826086956522, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790551, Length=214, Percent_Identity=26.1682242990654, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323034, Length=142, Percent_Identity=34.5070422535211, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322044, Length=128, Percent_Identity=38.28125, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322000, Length=124, Percent_Identity=37.0967741935484, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 90966; Mature: 90966

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQGSWSIKTFLLAGSVAIVILLIVNVSITQYVSNTKLDLSRLENEVVVSANTLLMIRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECEEEEEEE
SEKDFISRIDPKYTFQVLDFKAKLVQQLTQIDRLLTQSNLDISYNNQRVLEELDDYIDTF
CCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHH
SMLINEIYSINGTSAEQGLLARFEHNSNELQLLTNQLKDTELLELIIANQLTVFDFFKSF
HHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
DSRLLTRITHQFKIIEQHIIDKPQSTLPLQQAFLSFEHAFFQLQSSMKRRGYSESLGYHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TLRQNVHSVERSLETLFNTIPLKVDKSVSGLVVTQSIVELLLVLLIIGILYYIFYAITRM
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKGLIFAREKEQQANSAKSSFLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATIN
HCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SSSQTLLMLINDILDLSKIESGHLEFCPHTCNIKEVIYDTAALITPKAQQKNIVLRIIIG
CCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEC
DEVPTYICADEQKLRQILMNLASNAIKFTRSGSVSFKLVVKAEEGENIRLLFSVKDTGVG
CCCCCEEECCHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEECCCCCEEEEEEEECCCCC
IDKNKHAKIFEEFQQEESNTSIDYGGTGLGLSIATKMVKIMGGEIMISSDKGVGSEFHFI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEE
LAFPKQEERVCERLDSQHLIYCAIKHSPLLVQNIIDSNHQLDKITKVSEIRPLLKPSSVI
EECCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEE
VLNVDDISDKQVLVDIAKECSLTPIIIARANNSINTDFGELVAGYITLPLLGERLNTLIK
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVSHCQKQEEDLVPLNSDSQKSKKIVLVVEDNKVNQQVVSINLKKLNLPYLIANDGREAL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCEEEECCCHHHH
ENYKRHIGGVSVILMDCMMPIMDGFEATRAIRIFEKEEGEPRVTIIALTASILDDDIQKC
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCCCEEEEEEEHHHHHHHHHHH
FESGMDDYLPKPFKRDVLVRKLAKQH
HHCCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKQGSWSIKTFLLAGSVAIVILLIVNVSITQYVSNTKLDLSRLENEVVVSANTLLMIRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECEEEEEEE
SEKDFISRIDPKYTFQVLDFKAKLVQQLTQIDRLLTQSNLDISYNNQRVLEELDDYIDTF
CCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHH
SMLINEIYSINGTSAEQGLLARFEHNSNELQLLTNQLKDTELLELIIANQLTVFDFFKSF
HHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
DSRLLTRITHQFKIIEQHIIDKPQSTLPLQQAFLSFEHAFFQLQSSMKRRGYSESLGYHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TLRQNVHSVERSLETLFNTIPLKVDKSVSGLVVTQSIVELLLVLLIIGILYYIFYAITRM
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKGLIFAREKEQQANSAKSSFLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATIN
HCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SSSQTLLMLINDILDLSKIESGHLEFCPHTCNIKEVIYDTAALITPKAQQKNIVLRIIIG
CCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEC
DEVPTYICADEQKLRQILMNLASNAIKFTRSGSVSFKLVVKAEEGENIRLLFSVKDTGVG
CCCCCEEECCHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEECCCCCEEEEEEEECCCCC
IDKNKHAKIFEEFQQEESNTSIDYGGTGLGLSIATKMVKIMGGEIMISSDKGVGSEFHFI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEE
LAFPKQEERVCERLDSQHLIYCAIKHSPLLVQNIIDSNHQLDKITKVSEIRPLLKPSSVI
EECCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEE
VLNVDDISDKQVLVDIAKECSLTPIIIARANNSINTDFGELVAGYITLPLLGERLNTLIK
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVSHCQKQEEDLVPLNSDSQKSKKIVLVVEDNKVNQQVVSINLKKLNLPYLIANDGREAL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCEEEECCCHHHH
ENYKRHIGGVSVILMDCMMPIMDGFEATRAIRIFEKEEGEPRVTIIALTASILDDDIQKC
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCCCEEEEEEEHHHHHHHHHHH
FESGMDDYLPKPFKRDVLVRKLAKQH
HHCCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]