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The map label for this gene is lolA

Identifier: 157962053

GI number: 157962053

Start: 2704167

End: 2704793

Strand: Reverse

Name: lolA

Synonym: Spea_2232

Alternate gene names: 157962053

Gene position: 2704793-2704167 (Counterclockwise)

Preceding gene: 157962054

Following gene: 157962052

Centisome position: 52.27

GC content: 40.03

Gene sequence:

>627_bases
ATGAAAAAGATTTTCACGATAGCAGCCTTGAGTCTGCCATTGTTTTGCCACCTTCCAGCTATGGCTGGAGGGCAGGATGA
TTTAAAAGTAAAGCTAATGGAAATTAACACCTTAAAAGCGAACTTTAATCAAACTGTGACAGATATAAATCAAAAAGTTA
TTCAAACAGGCGAGGGCGTATTCGCACTTTCACACCCTAACCAGTTTTATTGGCATCTGACTGCACCCGATGAGTCATTG
ATCGTTGCCGATGGCACGGATGTGTGGATCTATAATCCATTTGCAGAAGAAGTCTCTGTTATGGATATAAATCAGGCCAT
CAATGCATCTCCTATCGCTTTATTAGTTCATTCCGATGACGCCACATGGTCGCAATATGATGTGGTAAATAACGGCGACT
GTTTTGATATTAGCCCCAGAGATAAAGATAGCGGCGTATCGGAAGTTGAGGTTTGCTTCAACCAACAGCAATTGACTAAA
ATGGTCTTAAAAGATCAACAGGGTAATACCAGTGATTTTACCCTGACGAATCAAACTCCTATTGCAGAGAATGACAAAGA
TCTGTTTAAGTTTATCGTACCTGATGATGTCGATATCGATGATCAGCGTTTAAAATCGCAAAATTAA

Upstream 100 bases:

>100_bases
ACTAGCGCCACCACCGCCAAAAGATTACGTATAAAACAGTTTGAATACGACTAATTAGATTTAGCGTTAAGCCTAAATAT
GGCTTTTCTCGGAGAGTATA

Downstream 100 bases:

>100_bases
GAGAAGTCAGTGTCTAGTTTCAGTTTTGATTTCGCACCGGATTTTAGGCCCTTAGCCGCTAGGATGAGGCCGGAAACATT
AGAGCAATATATAGGTCAGA

Product: outer membrane lipoprotein carrier protein LolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKKIFTIAALSLPLFCHLPAMAGGQDDLKVKLMEINTLKANFNQTVTDINQKVIQTGEGVFALSHPNQFYWHLTAPDESL
IVADGTDVWIYNPFAEEVSVMDINQAINASPIALLVHSDDATWSQYDVVNNGDCFDISPRDKDSGVSEVEVCFNQQQLTK
MVLKDQQGNTSDFTLTNQTPIAENDKDLFKFIVPDDVDIDDQRLKSQN

Sequences:

>Translated_208_residues
MKKIFTIAALSLPLFCHLPAMAGGQDDLKVKLMEINTLKANFNQTVTDINQKVIQTGEGVFALSHPNQFYWHLTAPDESL
IVADGTDVWIYNPFAEEVSVMDINQAINASPIALLVHSDDATWSQYDVVNNGDCFDISPRDKDSGVSEVEVCFNQQQLTK
MVLKDQQGNTSDFTLTNQTPIAENDKDLFKFIVPDDVDIDDQRLKSQN
>Mature_208_residues
MKKIFTIAALSLPLFCHLPAMAGGQDDLKVKLMEINTLKANFNQTVTDINQKVIQTGEGVFALSHPNQFYWHLTAPDESL
IVADGTDVWIYNPFAEEVSVMDINQAINASPIALLVHSDDATWSQYDVVNNGDCFDISPRDKDSGVSEVEVCFNQQQLTK
MVLKDQQGNTSDFTLTNQTPIAENDKDLFKFIVPDDVDIDDQRLKSQN

Specific function: Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the l

COG id: COG2834

COG function: function code M; Outer membrane lipoprotein-sorting protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolA family

Homologues:

Organism=Escherichia coli, GI145693108, Length=207, Percent_Identity=32.3671497584541, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): LOLA_SHEPA (A8H4R5)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001502087.1
- ProteinModelPortal:   A8H4R5
- SMR:   A8H4R5
- STRING:   A8H4R5
- GeneID:   5662625
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_2232
- NMPDR:   fig|398579.3.peg.1911
- eggNOG:   COG2834
- HOGENOM:   HBG688940
- OMA:   VDIDDQR
- ProtClustDB:   CLSK906679
- BioCyc:   SPEA398579:SPEA_2232-MONOMER
- HAMAP:   MF_00240
- InterPro:   IPR004564
- InterPro:   IPR018323
- TIGRFAMs:   TIGR00547

Pfam domain/function: PF03548 LolA

EC number: NA

Molecular weight: Translated: 23241; Mature: 23241

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFTIAALSLPLFCHLPAMAGGQDDLKVKLMEINTLKANFNQTVTDINQKVIQTGEGV
CCCEEEEHHHHCCCEEECCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCE
FALSHPNQFYWHLTAPDESLIVADGTDVWIYNPFAEEVSVMDINQAINASPIALLVHSDD
EEEECCCEEEEEEECCCCCEEEECCCEEEEECCHHHCEEEEECCCCCCCCCEEEEEECCC
ATWSQYDVVNNGDCFDISPRDKDSGVSEVEVCFNQQQLTKMVLKDQQGNTSDFTLTNQTP
CCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCC
IAENDKDLFKFIVPDDVDIDDQRLKSQN
CCCCCCHHEEEECCCCCCCCHHHHCCCC
>Mature Secondary Structure
MKKIFTIAALSLPLFCHLPAMAGGQDDLKVKLMEINTLKANFNQTVTDINQKVIQTGEGV
CCCEEEEHHHHCCCEEECCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCE
FALSHPNQFYWHLTAPDESLIVADGTDVWIYNPFAEEVSVMDINQAINASPIALLVHSDD
EEEECCCEEEEEEECCCCCEEEECCCEEEEECCHHHCEEEEECCCCCCCCCEEEEEECCC
ATWSQYDVVNNGDCFDISPRDKDSGVSEVEVCFNQQQLTKMVLKDQQGNTSDFTLTNQTP
CCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCC
IAENDKDLFKFIVPDDVDIDDQRLKSQN
CCCCCCHHEEEECCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA