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The map label for this gene is codB [H]

Identifier: 157962009

GI number: 157962009

Start: 2652703

End: 2653968

Strand: Reverse

Name: codB [H]

Synonym: Spea_2188

Alternate gene names: 157962009

Gene position: 2653968-2652703 (Counterclockwise)

Preceding gene: 157962011

Following gene: 157962008

Centisome position: 51.29

GC content: 45.73

Gene sequence:

>1266_bases
ATGGCAAGCGATAATAATTACAGTTTAAGTCCGGTTCCTCAATCGGGCCGTAAAGGTGTTTTCTCTCTGACGATGGTGAT
GCTGGGATTAACCTTTTTTTCAGCAAGTATGTGGACGGGAGGAACGCTCGGCACAGGCCTCTCTTTTAATGATTTTGTTC
TAGCAGTTCTTATTGGTAATTTAATTCTCGGTATCTATACTTCATTTCTTGGTTACATCGGCGCTCAAACAGGCCTCTCT
ACTCATCTCCTCGCGCGTTACTCATTCGGTTTAAAAGGTTCTTGGCTACCCTCGCTACTTCTCGGTGGCACGCAAGTTGG
TTGGTTTGGTGTTGGCGTGGCAATGTTTGCTATTCCGGTACAGAAAGCCACAGGTATAGATACCAACCTGCTGATTATTG
TCTCGGGTCTACTGATGACCTTTACCGTGTACTTCGGTATTGGCGCGATTATGATTTTGTCAGCCATCGCGGTTCCAGCC
ATCGCCCTATTTGGCGGATTCTCGGTACTACAAGCGGTCGACACTATGGGCGGCATGCAAGGCTTAATGGCGGTCGAGCC
TGAAAATCCAATCGATTTTAATACCGCTCTGGTATTGGTTGTCGGCTCATTTATCTCAGCCGGTACACTGACCGCAGATT
TTGTTCGCTTCGGTAAAAATCCTAAAACTGCCGTGTTTGTCACCATGTTTGCCTTCTTCGTCGGTAATTCGTTGATGTTT
ATCTTCGGCGCAGCGGGCGCTGCGGCAACGGGGCAAGCTGATATCTCTGAGGTTATGATTGCTCAGGGACTATTAATCCC
AGCCATTATCATCTTGGGTCTTAATATTTGGACCACCAATGATAATGCGCTGTATGCTTCGGGCTTAGGCTTTTCAAACA
TTACCGGCTTGCCGAGTAAGTACCTGTCAATTTTTAACGGCGTGATCGGCACTCTGTGCGCGCTATGGCTATATAATAAC
TTTGTCGGTTGGTTAACCTTCCTATCGGCAGCTATCCCACCTATTGGTGGCATTATCATCGCCGATTACTTACGTAACCG
TGAAAAATATAAAGACTTCGCCAATGCTAAGTTTTCAACGGTAAATTGGGCTGCGATTAGCGGTGTCGTGATAGGCGTGC
TAGCAGGCCACCTATTACCAGGCATAGTGCCAATTAATGCCGTACTTGCGGGTGCCATCAGCTATTTATTAATAGATGAA
GCGCTAAAACGCATGAATAAACAAAAATTAGTTAAATCAGATTGTAAAAATGCTACCAATGCATAA

Upstream 100 bases:

>100_bases
GTTTGCCTGATGCGCACAGACTCGAGCTTGCTTTCTATGCTTGAGTGATTTTGCAATTTCGGTAGGCCGTGAATAATAAT
TACATGACCAGAGGCAATAA

Downstream 100 bases:

>100_bases
GGAAAACACCATGTCATCAGCAAGTATGTTGATCAAAAATGTGACTCTAAAAGGTCAAGAAGGCCTTCAACAATTGTTGA
TTGAAGACGGTAAGTTCAAA

Product: cytosine permease

Products: Proton [Cytoplasm]; cytosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MASDNNYSLSPVPQSGRKGVFSLTMVMLGLTFFSASMWTGGTLGTGLSFNDFVLAVLIGNLILGIYTSFLGYIGAQTGLS
THLLARYSFGLKGSWLPSLLLGGTQVGWFGVGVAMFAIPVQKATGIDTNLLIIVSGLLMTFTVYFGIGAIMILSAIAVPA
IALFGGFSVLQAVDTMGGMQGLMAVEPENPIDFNTALVLVVGSFISAGTLTADFVRFGKNPKTAVFVTMFAFFVGNSLMF
IFGAAGAAATGQADISEVMIAQGLLIPAIIILGLNIWTTNDNALYASGLGFSNITGLPSKYLSIFNGVIGTLCALWLYNN
FVGWLTFLSAAIPPIGGIIIADYLRNREKYKDFANAKFSTVNWAAISGVVIGVLAGHLLPGIVPINAVLAGAISYLLIDE
ALKRMNKQKLVKSDCKNATNA

Sequences:

>Translated_421_residues
MASDNNYSLSPVPQSGRKGVFSLTMVMLGLTFFSASMWTGGTLGTGLSFNDFVLAVLIGNLILGIYTSFLGYIGAQTGLS
THLLARYSFGLKGSWLPSLLLGGTQVGWFGVGVAMFAIPVQKATGIDTNLLIIVSGLLMTFTVYFGIGAIMILSAIAVPA
IALFGGFSVLQAVDTMGGMQGLMAVEPENPIDFNTALVLVVGSFISAGTLTADFVRFGKNPKTAVFVTMFAFFVGNSLMF
IFGAAGAAATGQADISEVMIAQGLLIPAIIILGLNIWTTNDNALYASGLGFSNITGLPSKYLSIFNGVIGTLCALWLYNN
FVGWLTFLSAAIPPIGGIIIADYLRNREKYKDFANAKFSTVNWAAISGVVIGVLAGHLLPGIVPINAVLAGAISYLLIDE
ALKRMNKQKLVKSDCKNATNA
>Mature_420_residues
ASDNNYSLSPVPQSGRKGVFSLTMVMLGLTFFSASMWTGGTLGTGLSFNDFVLAVLIGNLILGIYTSFLGYIGAQTGLST
HLLARYSFGLKGSWLPSLLLGGTQVGWFGVGVAMFAIPVQKATGIDTNLLIIVSGLLMTFTVYFGIGAIMILSAIAVPAI
ALFGGFSVLQAVDTMGGMQGLMAVEPENPIDFNTALVLVVGSFISAGTLTADFVRFGKNPKTAVFVTMFAFFVGNSLMFI
FGAAGAAATGQADISEVMIAQGLLIPAIIILGLNIWTTNDNALYASGLGFSNITGLPSKYLSIFNGVIGTLCALWLYNNF
VGWLTFLSAAIPPIGGIIIADYLRNREKYKDFANAKFSTVNWAAISGVVIGVLAGHLLPGIVPINAVLAGAISYLLIDEA
LKRMNKQKLVKSDCKNATNA

Specific function: Required for cytosine transport into the cell [H]

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI1786530, Length=417, Percent_Identity=73.8609112709832, Blast_Score=601, Evalue=1e-173,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 44261; Mature: 44129

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure 
ASDNNYSLSPVPQSGRKGVFSLTMVMLGLTFFSASMWTGGTLGTGLSFNDFVLAVLIGN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LILGIYTSFLGYIGAQTGLSTHLLARYSFGLKGSWLPSLLLGGTQVGWFGVGVAMFAIPV
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
QKATGIDTNLLIIVSGLLMTFTVYFGIGAIMILSAIAVPAIALFGGFSVLQAVDTMGGMQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLMAVEPENPIDFNTALVLVVGSFISAGTLTADFVRFGKNPKTAVFVTMFAFFVGNSLMF
CCEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
IFGAAGAAATGQADISEVMIAQGLLIPAIIILGLNIWTTNDNALYASGLGFSNITGLPSK
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCCCCCCCCHHH
YLSIFNGVIGTLCALWLYNNFVGWLTFLSAAIPPIGGIIIADYLRNREKYKDFANAKFST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHCCCCCC
VNWAAISGVVIGVLAGHLLPGIVPINAVLAGAISYLLIDEALKRMNKQKLVKSDCKNATN
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; cytosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + cytosine [Periplasm] = Proton [Cytoplasm] + cytosine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]