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The map label for this gene is fadH [C]

Identifier: 157961967

GI number: 157961967

Start: 2598332

End: 2600452

Strand: Reverse

Name: fadH [C]

Synonym: Spea_2146

Alternate gene names: 157961967

Gene position: 2600452-2598332 (Counterclockwise)

Preceding gene: 157961968

Following gene: 157961957

Centisome position: 50.25

GC content: 46.86

Gene sequence:

>2121_bases
ATGGCGAACTTCGAACATTTACTAAAACCCGGAAAAATAGGCAGTTTGACGCTTAAAAACCGCATGGTAATGGCTCCTAT
GGGATCCAATTTTGCGGAGTCTAATGGCCATTGTGGTGAACGCATTCAAGCTTTCTACGAAGAAAGGGCTAAAGGCGGCG
CTGCACTGCTAACCATGGGGGTGGGTGCTATTGCTTACCCTGCGGGTACTGCAGAGCCTTACCAAGTTGGTATTTCATCT
GATGAGTTTATTCCCGGTCTAAAGCAGTTAACCGACCGCGTACATAAGCATGGTGCCAAAATCGCTATCCAGTTACAGCA
TGCAGGTAAAACTGCGGTAAGGGATTTAGCCGAAGGCAGGGATTTATGGGTGCCATCAATGCCACCCGCGACCAATAGCG
ATATGATGAAGACCTTAACCCATAGTGAACTGGCCTCTTTCATTCGCCCAAAAGCAGGCGGGCAGATTAAAATTCGGGTT
ATGGACCAAGCAGATATCAAGCAAATGGTAGCTTGGTTCGCATCTGCCGCACTTAGAGCTAAACAAGCGGGCTTTGATGG
AGTCGAGATTCATGCTGCTCACAGCTACATTATTGCAGGCTTTTTATCGGCTTATTACAACAAACGTGATGATAAGTATG
GCGGCAGCCTCGAAAACAGAGCGCGGTTGTTGCTCGAAGTGATAGCGGCTGTGCGGCAACAAGTTGGTAATGATTACCCA
GTTTGGTTAAGGCTCGATGCGGCAGAATTACGCACTCCTGGCGGGATCACAATTGAAGACTGCATCGAGGTCGCCAAGCT
GGTTGAAGCTGCAGGCCTCAATGCCTTGAGTATTTCAGCCTATGCCAATACTAATCGAGGCGATGCATTTACCGAAGCGC
CCTTAGTACAAAAGCCTGCAGGTTTTCTTCATTGGGCCGCAGAGGTGAGTAAACATGTTTCAATCCCTGTTACTGCGGTT
GGTCGTTTAGAGCCCGATGTTGCCGATAAAGCGATTGGCAGTGGCAAAATAGCTTTTGTTGCAATGGCGAGAAAACTGCT
AGCGGACCCAGAGCTGCCAAACAAAATCATCAATAACCGCGCTGAGGAGATCCGTCCCTGCATATATTGTTATGTCTGCG
TTAGCCAAATTTTTATTAATCAGCGAGTGAAGTGCGCGGTAAACCCGAGAACAGGACATGAATCAACACTGAACATCATT
GCCACTGACAAAGTAAAACATATCGTTATTGTCGGAGCTGGCCCTAGTGGCATGGAAGCTGCAAAAGTTGCCAGTGACCG
TGGTCATAGAGTGACGCTGATTGAACGAGGGTCACGCCTAGGTGGCACTCTGTTTTTTGCCTCAATGACCTACCCTGAAA
ATGGTAAATTACTCGAATACCAAATTAATCAGCTGAAGCAATCTAAGGTGGATATTCGCCTTAATACCGAAGCATCGGTT
GAATTACTTAGCCAATTGCAACCTGATGAAATTCTACTTGCGGTGGGCGCCCGCCGCACGAGTCCAAATATCGTCGGTTC
GCAACTGCAACACGTATGGTCTGGAGATGAATTGCGTCTACTCATGACCGGCGAAGGCAAAGATATCGCTAAGCAAAAGC
TGTCACTTTCACAACGAGCGATGTTCAATGCCGGATCAGCAAGTGGCGTCACATCCAGTTTAGATGCCTGCCGTCAGCTG
TCTAAGTTATGGATGCCTCTGGGTAAAAAGATTACAGTTATTGGTGGGGGTTTAGTCGGATTAGAATTGGCAGAATTCTT
AGCGCTTCGAGGACGGGATGTGACCATCATCGAACCAACAAGAGACTTAGGCAGCGAACTGTCGATTGTACGTCGTTGGC
GGATCTTGCAAGAGCTCAATGAATTAGGAGTAAAAATTCTTACAAATTCAACTGTGAATAAAATTGATGCCAATAATGTA
ACCTTCACAGATAAAGAAGATGCAGCACAAACATTAGCAAGTGATAGCGTCATTATGGCTCAAGGCGCAGCGCCTAATAG
CGACTTTGAGCAGATGTTGGTCGCGGAAGGCTTCAAAATCAGCTCAATCGGAGACGGTAGTGCTGTTAATTATATTGAAG
GCGCTATTAGTTCTGGTATGAAAGCAGCGATTGAACTATAG

Upstream 100 bases:

>100_bases
TAGGTATTATGGGTTAATCGTGTTCCGAACAGCCTGCTACCTAATGACGAATAATTAAAAATAATAACGATCACAAAGAC
AAAAATTAGAGAGAGTATCA

Downstream 100 bases:

>100_bases
CGCATAACTCAGATGTAGTAGCTCAGATGTAGTAGCTCAGACGAGTATCAAATACGCGACACGCTATGCACTCCTCAGTG
ACAACATAAAGGCCGCAATC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MANFEHLLKPGKIGSLTLKNRMVMAPMGSNFAESNGHCGERIQAFYEERAKGGAALLTMGVGAIAYPAGTAEPYQVGISS
DEFIPGLKQLTDRVHKHGAKIAIQLQHAGKTAVRDLAEGRDLWVPSMPPATNSDMMKTLTHSELASFIRPKAGGQIKIRV
MDQADIKQMVAWFASAALRAKQAGFDGVEIHAAHSYIIAGFLSAYYNKRDDKYGGSLENRARLLLEVIAAVRQQVGNDYP
VWLRLDAAELRTPGGITIEDCIEVAKLVEAAGLNALSISAYANTNRGDAFTEAPLVQKPAGFLHWAAEVSKHVSIPVTAV
GRLEPDVADKAIGSGKIAFVAMARKLLADPELPNKIINNRAEEIRPCIYCYVCVSQIFINQRVKCAVNPRTGHESTLNII
ATDKVKHIVIVGAGPSGMEAAKVASDRGHRVTLIERGSRLGGTLFFASMTYPENGKLLEYQINQLKQSKVDIRLNTEASV
ELLSQLQPDEILLAVGARRTSPNIVGSQLQHVWSGDELRLLMTGEGKDIAKQKLSLSQRAMFNAGSASGVTSSLDACRQL
SKLWMPLGKKITVIGGGLVGLELAEFLALRGRDVTIIEPTRDLGSELSIVRRWRILQELNELGVKILTNSTVNKIDANNV
TFTDKEDAAQTLASDSVIMAQGAAPNSDFEQMLVAEGFKISSIGDGSAVNYIEGAISSGMKAAIEL

Sequences:

>Translated_706_residues
MANFEHLLKPGKIGSLTLKNRMVMAPMGSNFAESNGHCGERIQAFYEERAKGGAALLTMGVGAIAYPAGTAEPYQVGISS
DEFIPGLKQLTDRVHKHGAKIAIQLQHAGKTAVRDLAEGRDLWVPSMPPATNSDMMKTLTHSELASFIRPKAGGQIKIRV
MDQADIKQMVAWFASAALRAKQAGFDGVEIHAAHSYIIAGFLSAYYNKRDDKYGGSLENRARLLLEVIAAVRQQVGNDYP
VWLRLDAAELRTPGGITIEDCIEVAKLVEAAGLNALSISAYANTNRGDAFTEAPLVQKPAGFLHWAAEVSKHVSIPVTAV
GRLEPDVADKAIGSGKIAFVAMARKLLADPELPNKIINNRAEEIRPCIYCYVCVSQIFINQRVKCAVNPRTGHESTLNII
ATDKVKHIVIVGAGPSGMEAAKVASDRGHRVTLIERGSRLGGTLFFASMTYPENGKLLEYQINQLKQSKVDIRLNTEASV
ELLSQLQPDEILLAVGARRTSPNIVGSQLQHVWSGDELRLLMTGEGKDIAKQKLSLSQRAMFNAGSASGVTSSLDACRQL
SKLWMPLGKKITVIGGGLVGLELAEFLALRGRDVTIIEPTRDLGSELSIVRRWRILQELNELGVKILTNSTVNKIDANNV
TFTDKEDAAQTLASDSVIMAQGAAPNSDFEQMLVAEGFKISSIGDGSAVNYIEGAISSGMKAAIEL
>Mature_705_residues
ANFEHLLKPGKIGSLTLKNRMVMAPMGSNFAESNGHCGERIQAFYEERAKGGAALLTMGVGAIAYPAGTAEPYQVGISSD
EFIPGLKQLTDRVHKHGAKIAIQLQHAGKTAVRDLAEGRDLWVPSMPPATNSDMMKTLTHSELASFIRPKAGGQIKIRVM
DQADIKQMVAWFASAALRAKQAGFDGVEIHAAHSYIIAGFLSAYYNKRDDKYGGSLENRARLLLEVIAAVRQQVGNDYPV
WLRLDAAELRTPGGITIEDCIEVAKLVEAAGLNALSISAYANTNRGDAFTEAPLVQKPAGFLHWAAEVSKHVSIPVTAVG
RLEPDVADKAIGSGKIAFVAMARKLLADPELPNKIINNRAEEIRPCIYCYVCVSQIFINQRVKCAVNPRTGHESTLNIIA
TDKVKHIVIVGAGPSGMEAAKVASDRGHRVTLIERGSRLGGTLFFASMTYPENGKLLEYQINQLKQSKVDIRLNTEASVE
LLSQLQPDEILLAVGARRTSPNIVGSQLQHVWSGDELRLLMTGEGKDIAKQKLSLSQRAMFNAGSASGVTSSLDACRQLS
KLWMPLGKKITVIGGGLVGLELAEFLALRGRDVTIIEPTRDLGSELSIVRRWRILQELNELGVKILTNSTVNKIDANNVT
FTDKEDAAQTLASDSVIMAQGAAPNSDFEQMLVAEGFKISSIGDGSAVNYIEGAISSGMKAAIEL

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=730, Percent_Identity=29.4520547945205, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1787939, Length=371, Percent_Identity=29.3800539083558, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17565138, Length=199, Percent_Identity=30.6532663316583, Blast_Score=102, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=274, Percent_Identity=27.3722627737226, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=278, Percent_Identity=27.3381294964029, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=276, Percent_Identity=28.6231884057971, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=366, Percent_Identity=27.3224043715847, Blast_Score=100, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=271, Percent_Identity=25.0922509225092, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=371, Percent_Identity=26.1455525606469, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321973, Length=236, Percent_Identity=31.3559322033898, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=238, Percent_Identity=30.2521008403361, Blast_Score=87, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 76189; Mature: 76057

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFEHLLKPGKIGSLTLKNRMVMAPMGSNFAESNGHCGERIQAFYEERAKGGAALLTMG
CCCHHHHCCCCCCCEEEECCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
VGAIAYPAGTAEPYQVGISSDEFIPGLKQLTDRVHKHGAKIAIQLQHAGKTAVRDLAEGR
CCCEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCC
DLWVPSMPPATNSDMMKTLTHSELASFIRPKAGGQIKIRVMDQADIKQMVAWFASAALRA
CEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
KQAGFDGVEIHAAHSYIIAGFLSAYYNKRDDKYGGSLENRARLLLEVIAAVRQQVGNDYP
HHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VWLRLDAAELRTPGGITIEDCIEVAKLVEAAGLNALSISAYANTNRGDAFTEAPLVQKPA
EEEEEEHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
GFLHWAAEVSKHVSIPVTAVGRLEPDVADKAIGSGKIAFVAMARKLLADPELPNKIINNR
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCC
AEEIRPCIYCYVCVSQIFINQRVKCAVNPRTGHESTLNIIATDKVKHIVIVGAGPSGMEA
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCEEEEEEEECCCCCCHH
AKVASDRGHRVTLIERGSRLGGTLFFASMTYPENGKLLEYQINQLKQSKVDIRLNTEASV
HHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHCCEEEEECCHHHH
ELLSQLQPDEILLAVGARRTSPNIVGSQLQHVWSGDELRLLMTGEGKDIAKQKLSLSQRA
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
MFNAGSASGVTSSLDACRQLSKLWMPLGKKITVIGGGLVGLELAEFLALRGRDVTIIEPT
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCC
RDLGSELSIVRRWRILQELNELGVKILTNSTVNKIDANNVTFTDKEDAAQTLASDSVIMA
HHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEECCCHHHHHHHHCCCEEEE
QGAAPNSDFEQMLVAEGFKISSIGDGSAVNYIEGAISSGMKAAIEL
ECCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
ANFEHLLKPGKIGSLTLKNRMVMAPMGSNFAESNGHCGERIQAFYEERAKGGAALLTMG
CCHHHHCCCCCCCEEEECCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
VGAIAYPAGTAEPYQVGISSDEFIPGLKQLTDRVHKHGAKIAIQLQHAGKTAVRDLAEGR
CCCEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCC
DLWVPSMPPATNSDMMKTLTHSELASFIRPKAGGQIKIRVMDQADIKQMVAWFASAALRA
CEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
KQAGFDGVEIHAAHSYIIAGFLSAYYNKRDDKYGGSLENRARLLLEVIAAVRQQVGNDYP
HHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VWLRLDAAELRTPGGITIEDCIEVAKLVEAAGLNALSISAYANTNRGDAFTEAPLVQKPA
EEEEEEHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
GFLHWAAEVSKHVSIPVTAVGRLEPDVADKAIGSGKIAFVAMARKLLADPELPNKIINNR
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCC
AEEIRPCIYCYVCVSQIFINQRVKCAVNPRTGHESTLNIIATDKVKHIVIVGAGPSGMEA
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCEEEEEEEECCCCCCHH
AKVASDRGHRVTLIERGSRLGGTLFFASMTYPENGKLLEYQINQLKQSKVDIRLNTEASV
HHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHCCEEEEECCHHHH
ELLSQLQPDEILLAVGARRTSPNIVGSQLQHVWSGDELRLLMTGEGKDIAKQKLSLSQRA
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
MFNAGSASGVTSSLDACRQLSKLWMPLGKKITVIGGGLVGLELAEFLALRGRDVTIIEPT
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCC
RDLGSELSIVRRWRILQELNELGVKILTNSTVNKIDANNVTFTDKEDAAQTLASDSVIMA
HHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEECCCHHHHHHHHCCCEEEE
QGAAPNSDFEQMLVAEGFKISSIGDGSAVNYIEGAISSGMKAAIEL
ECCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]