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The map label for this gene is yfbQ [H]

Identifier: 157961869

GI number: 157961869

Start: 2481285

End: 2482499

Strand: Reverse

Name: yfbQ [H]

Synonym: Spea_2048

Alternate gene names: 157961869

Gene position: 2482499-2481285 (Counterclockwise)

Preceding gene: 157961870

Following gene: 157961868

Centisome position: 47.97

GC content: 43.21

Gene sequence:

>1215_bases
ATGCGCCCTATTATCAAATCCAACAAATTAGATTCTGTATGTTACGACATTCGTGGTCCAGTACATAAAGAAGCCCGTCG
TCTTGAAGATGAAGGTCACCGTATTCTTAAACTGAATATTGGTAACCCAGCCCCATTTGGATTTGAGGCACCAGAAGAGA
TCGTGCGAGATGTAATTTTAAATCTACCGAGCGCGCAAGGCTACTGTGAGTCGAAAGGTCTGTTTTCTGCGCGTAAAGCG
ATCGTGCAACATTACCAGTCTCAGGGGATCTTCGGTGTCGATATTGAAGACATCTATATCGGTAATGGTGTATCTGAGCT
TATTGTTATGGCTATGCAAGGTCTACTGAATAGTGGTGATGAAGTTTTGGTTCCTTCACCTGACTACCCTTTATGGACTG
CAGCTGTGCACCTGTCTGGCGGTAAAGCACAGCATTACCGTTGTGATGAAGAATCAGATTGGTTCCCAGATCTTGAAGAT
ATTAAAAGCAAAATTACCCCAAGAACTCGCGGTCTTGTGTTGATTAACCCGAATAATCCAACAGGAGCAGTTTACTCCAA
GGAACTGATCCTTGAAGTGATCGAGCTTTGTCGTCAAAACGATATGATTCTGTTCGCCGACGAAATCTACGATAAGATCT
TGTATGATGAAGCTGTTCATATTCCTGCTGCAAGTCTATCGGATGACATCTTAACCGTTACCTTTAACGGCCTATCAAAG
GTTTACCGTGCGGCCGGCTTCCGCGTGGGATGGATGATGCTAACTGGTAACAAAAAAGCGGCAAAGAGTTATGTTGAAGG
CTTAGATATGCTGGCATCAATGCGTCTGTGCGCAAACGTGCCAAGTCAGCATGCTATTCAAACAGCATTGGGTGGCTATC
AGAGCATTCAAGAGTTAGTGCAGCCTGGCGGACGTTTACGCGTGCAGCGTGATGCCTGTGTTGAAATGCTCAACTCTATT
CCTGGTGTCAGTGTTAAAACGCCGAAAGGTGCTTTATATGCGTTTGCTAAGTTAGATCAAAAGAAATTTAACCTGCGTGA
CGATGAACGCTTAGTGCTTGATTTACTAAAAGACCGTAAGATCTTATTAGTTCAAGGCTCGGCATTTAATTATCCAGAAC
CTGATCATGTTCGTGTCGTATTTTTGCCTTACAAAGAAGAACTAACCAAAGCCTTATCTGAATTTGGTGATTTCTTAGCT
AATTATAAGCAGTAA

Upstream 100 bases:

>100_bases
AGTGAATTAATCCTTTATTTGTTCTTTGAATTTAGCTATTGTGCCAGCAACAAAGTCAAAGAAGTGTTTGACTCGTATTT
TCAGCATTTTCGAGGTGTAG

Downstream 100 bases:

>100_bases
CTCACTAATAAAACACAGAAAAATAAAACGACCTCAGGGTCGTTTTTTGTTTTCTAACTATGCTAATCTGAAAGGGTTCA
ACTCCTAACTTCAAAGGGAT

Product: aminotransferase AlaT

Products: oxaloacetate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MRPIIKSNKLDSVCYDIRGPVHKEARRLEDEGHRILKLNIGNPAPFGFEAPEEIVRDVILNLPSAQGYCESKGLFSARKA
IVQHYQSQGIFGVDIEDIYIGNGVSELIVMAMQGLLNSGDEVLVPSPDYPLWTAAVHLSGGKAQHYRCDEESDWFPDLED
IKSKITPRTRGLVLINPNNPTGAVYSKELILEVIELCRQNDMILFADEIYDKILYDEAVHIPAASLSDDILTVTFNGLSK
VYRAAGFRVGWMMLTGNKKAAKSYVEGLDMLASMRLCANVPSQHAIQTALGGYQSIQELVQPGGRLRVQRDACVEMLNSI
PGVSVKTPKGALYAFAKLDQKKFNLRDDERLVLDLLKDRKILLVQGSAFNYPEPDHVRVVFLPYKEELTKALSEFGDFLA
NYKQ

Sequences:

>Translated_404_residues
MRPIIKSNKLDSVCYDIRGPVHKEARRLEDEGHRILKLNIGNPAPFGFEAPEEIVRDVILNLPSAQGYCESKGLFSARKA
IVQHYQSQGIFGVDIEDIYIGNGVSELIVMAMQGLLNSGDEVLVPSPDYPLWTAAVHLSGGKAQHYRCDEESDWFPDLED
IKSKITPRTRGLVLINPNNPTGAVYSKELILEVIELCRQNDMILFADEIYDKILYDEAVHIPAASLSDDILTVTFNGLSK
VYRAAGFRVGWMMLTGNKKAAKSYVEGLDMLASMRLCANVPSQHAIQTALGGYQSIQELVQPGGRLRVQRDACVEMLNSI
PGVSVKTPKGALYAFAKLDQKKFNLRDDERLVLDLLKDRKILLVQGSAFNYPEPDHVRVVFLPYKEELTKALSEFGDFLA
NYKQ
>Mature_404_residues
MRPIIKSNKLDSVCYDIRGPVHKEARRLEDEGHRILKLNIGNPAPFGFEAPEEIVRDVILNLPSAQGYCESKGLFSARKA
IVQHYQSQGIFGVDIEDIYIGNGVSELIVMAMQGLLNSGDEVLVPSPDYPLWTAAVHLSGGKAQHYRCDEESDWFPDLED
IKSKITPRTRGLVLINPNNPTGAVYSKELILEVIELCRQNDMILFADEIYDKILYDEAVHIPAASLSDDILTVTFNGLSK
VYRAAGFRVGWMMLTGNKKAAKSYVEGLDMLASMRLCANVPSQHAIQTALGGYQSIQELVQPGGRLRVQRDACVEMLNSI
PGVSVKTPKGALYAFAKLDQKKFNLRDDERLVLDLLKDRKILLVQGSAFNYPEPDHVRVVFLPYKEELTKALSEFGDFLA
NYKQ

Specific function: Unknown

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4507369, Length=380, Percent_Identity=25, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19263340, Length=467, Percent_Identity=24.6252676659529, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI215599424, Length=376, Percent_Identity=25.2659574468085, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4885351, Length=443, Percent_Identity=24.6049661399549, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI95147551, Length=424, Percent_Identity=24.5283018867925, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI169881279, Length=424, Percent_Identity=24.5283018867925, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56713256, Length=335, Percent_Identity=24.4776119402985, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI56713254, Length=335, Percent_Identity=24.4776119402985, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI169881281, Length=275, Percent_Identity=26.9090909090909, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI149944424, Length=390, Percent_Identity=24.3589743589744, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788627, Length=404, Percent_Identity=69.3069306930693, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI1788722, Length=398, Percent_Identity=27.1356783919598, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786816, Length=267, Percent_Identity=24.3445692883895, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787909, Length=309, Percent_Identity=20.7119741100324, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17567663, Length=377, Percent_Identity=26.790450928382, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71981209, Length=466, Percent_Identity=25.3218884120172, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71994472, Length=239, Percent_Identity=25.5230125523013, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994476, Length=239, Percent_Identity=25.5230125523013, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71981215, Length=294, Percent_Identity=24.4897959183673, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567369, Length=174, Percent_Identity=28.1609195402299, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323118, Length=379, Percent_Identity=28.4960422163588, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320317, Length=473, Percent_Identity=26.215644820296, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322401, Length=331, Percent_Identity=22.9607250755287, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18859735, Length=381, Percent_Identity=25.1968503937008, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI45551451, Length=472, Percent_Identity=25, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24641770, Length=472, Percent_Identity=25, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24641760, Length=472, Percent_Identity=25, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24641768, Length=472, Percent_Identity=25, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24641766, Length=472, Percent_Identity=25, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24641764, Length=472, Percent_Identity=25, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI28573069, Length=341, Percent_Identity=24.3401759530792, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646114, Length=341, Percent_Identity=24.3401759530792, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28573067, Length=341, Percent_Identity=24.3401759530792, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28573065, Length=341, Percent_Identity=24.3401759530792, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.1

Molecular weight: Translated: 45132; Mature: 45132

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIIKSNKLDSVCYDIRGPVHKEARRLEDEGHRILKLNIGNPAPFGFEAPEEIVRDVIL
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPSAQGYCESKGLFSARKAIVQHYQSQGIFGVDIEDIYIGNGVSELIVMAMQGLLNSGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCHHEEEECCCHHHHHHHHHHHHHCCCC
EVLVPSPDYPLWTAAVHLSGGKAQHYRCDEESDWFPDLEDIKSKITPRTRGLVLINPNNP
EEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCC
TGAVYSKELILEVIELCRQNDMILFADEIYDKILYDEAVHIPAASLSDDILTVTFNGLSK
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHH
VYRAAGFRVGWMMLTGNKKAAKSYVEGLDMLASMRLCANVPSQHAIQTALGGYQSIQELV
HHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
QPGGRLRVQRDACVEMLNSIPGVSVKTPKGALYAFAKLDQKKFNLRDDERLVLDLLKDRK
CCCCCEEECHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCE
ILLVQGSAFNYPEPDHVRVVFLPYKEELTKALSEFGDFLANYKQ
EEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPIIKSNKLDSVCYDIRGPVHKEARRLEDEGHRILKLNIGNPAPFGFEAPEEIVRDVIL
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPSAQGYCESKGLFSARKAIVQHYQSQGIFGVDIEDIYIGNGVSELIVMAMQGLLNSGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCHHEEEECCCHHHHHHHHHHHHHCCCC
EVLVPSPDYPLWTAAVHLSGGKAQHYRCDEESDWFPDLEDIKSKITPRTRGLVLINPNNP
EEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCC
TGAVYSKELILEVIELCRQNDMILFADEIYDKILYDEAVHIPAASLSDDILTVTFNGLSK
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHH
VYRAAGFRVGWMMLTGNKKAAKSYVEGLDMLASMRLCANVPSQHAIQTALGGYQSIQELV
HHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
QPGGRLRVQRDACVEMLNSIPGVSVKTPKGALYAFAKLDQKKFNLRDDERLVLDLLKDRK
CCCCCEEECHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCE
ILLVQGSAFNYPEPDHVRVVFLPYKEELTKALSEFGDFLANYKQ
EEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate; 2-oxoglutarate

Specific reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]