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The map label for this gene is ptsI [H]

Identifier: 157961815

GI number: 157961815

Start: 2417516

End: 2419219

Strand: Reverse

Name: ptsI [H]

Synonym: Spea_1994

Alternate gene names: 157961815

Gene position: 2419219-2417516 (Counterclockwise)

Preceding gene: 157961816

Following gene: 157961814

Centisome position: 46.75

GC content: 42.9

Gene sequence:

>1704_bases
ATGTCGATTACGGGAATCGTTGTTTCATCGGGTATAGCTTTCGGACAAGCCCTACATCTTAACTTACAGCACCAGAAAAT
CGATTATCGTGTTATCCCATTAGCCCAAGTCCCCATTGAAATTGAAAGCCTCAAAACAGCTTTTGAGCAACAAAAGCAAC
ATTTAACTCAAGGCCTCGCAAAATTAGATCCAAGCACAAGCCATTTTGAACTCATTGAGGCAGACATCCTCATTCTCGAT
GATGAAGAATTGCTAGCCCAAATTAAAGACGCTATCTCAAAGTTACAGATCAGAGCAGCTGCGGCCATTGAGCGGATCTT
TACTTATCAAGCATCAGAATTAGAGCAGTTAGATGATCCCTATTTAGCCAATCGAGCCGAAGATATATTGTGCTTGAGTA
GCCGCCTTATCGCGCGCTTACACGGCGCCCAACATCTGGACTTAAGCCAGCTAAATCAAGATACCATTATCTTCGCTCAT
GACTTAACACCAGCAGAGTTTGCCATGTTGCCTCTTAAGCATATTAAGGGCTTAGTGCTCAATACTGGTGGCATAACGAG
TCATACAGCCATTCTAGCTCGAAGTGCAGGGATCCCTGCGTTGCTTAGTTGCCAATTTGACGGCGAGCAGATCAAAAATG
GTACCCAAGTTGTTCTTAACGCTATCGAAGGTGAGCTCGTCATTAAGCCAAGTCCTGAACATCTAGCTCAACTATATAGG
CTGCAGCAACAAGATATTGAACGTAAGCAACAGTTATTGACCCTTAAAGATCTACCTACTGAGACTTTAGACGCTCATCA
GGTTTCATTATTGGCAAACGTTGGTAGCTTAAATGAAATTAGCCATTTGGCCGATGTAGGTGCGGAGGGAATTGGTCTGT
TTCGTACTGAATTTATGTTAATGAACACCAGTATCCTTCCTGATGAGAAAACCCAGTATCACTTCTATTGTGATGCGCTG
CATTTAATGGCTGGAAAAACCTTTACCATACGAACCTTAGATGTCGGCGCAGATAAAGAGCTACCTTGCTTAACGCCACT
GGTAGAAGACAATCCCGCATTAGGCATGAGAGGTATTCGCTATAGTTTGGCCAATCCTCAACTTTTTATCACCCAAGTGA
AAGCTATTTTACGGGCAGCAAATCATGGCCCAATCCGACTGATGTTCCCAATGATCAATCAGGTCGAAGAGCTAGAAGCA
TTATTTGACTTAATTGAGGCCTGTAAGCTCCAGCTAATTGAGGAAGAAAAAGGCTTCGGACAGTTAAGCTATGGCATAGT
CGTAGAGACGCCGGCAGCAGTACTAAACCTTGAATCCATGCTACCGCAACTGGATTTTATCAGTATAGGCACCAACGATC
TCACCCAGTACACTATGGCTGCGGATCGGACAAATCCTTCACTCACAAGCGAGTACCCTTCGCTTTCACCAGCAGTATTG
CACCTTATTAAAACAACGATAGATCTTGCTCAGCAACACCAAGTCAAGGTTTCACTCTGCGGAGAATTAGCCAGCGAGCC
TAAAGTTGCTCCTTTACTAGTAGGTATGGGGATCGATGAGCTAAGCGTTAATTTAAGTTCATTGTTAGAGGTAAAATCTG
CAATCCGTGATATAAGTTATCAAACCTGTCTAGCACTTGCGAAACATGCAATTAAAATAAAAAGAATCGAAGAATTAAAT
ATTTACATTAATGGTCTCAACTGA

Upstream 100 bases:

>100_bases
TATGGCGTATCGGTACGAGTCATTGCCGAGGGGGAACACGCTGATCAAGCGGTTGAAGAAGTGTCTGAATTACTCATTAC
ATTAAGTTAGGAGACTTGTT

Downstream 100 bases:

>100_bases
TCTCGATATAATGGCTATAGCCTTTATCAAATACAAATCAACATACCTAAGAGGCACAAGATAGATGGGATTTTTAAGCC
GCATTAGACGCCTAATTTCA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSITGIVVSSGIAFGQALHLNLQHQKIDYRVIPLAQVPIEIESLKTAFEQQKQHLTQGLAKLDPSTSHFELIEADILILD
DEELLAQIKDAISKLQIRAAAAIERIFTYQASELEQLDDPYLANRAEDILCLSSRLIARLHGAQHLDLSQLNQDTIIFAH
DLTPAEFAMLPLKHIKGLVLNTGGITSHTAILARSAGIPALLSCQFDGEQIKNGTQVVLNAIEGELVIKPSPEHLAQLYR
LQQQDIERKQQLLTLKDLPTETLDAHQVSLLANVGSLNEISHLADVGAEGIGLFRTEFMLMNTSILPDEKTQYHFYCDAL
HLMAGKTFTIRTLDVGADKELPCLTPLVEDNPALGMRGIRYSLANPQLFITQVKAILRAANHGPIRLMFPMINQVEELEA
LFDLIEACKLQLIEEEKGFGQLSYGIVVETPAAVLNLESMLPQLDFISIGTNDLTQYTMAADRTNPSLTSEYPSLSPAVL
HLIKTTIDLAQQHQVKVSLCGELASEPKVAPLLVGMGIDELSVNLSSLLEVKSAIRDISYQTCLALAKHAIKIKRIEELN
IYINGLN

Sequences:

>Translated_567_residues
MSITGIVVSSGIAFGQALHLNLQHQKIDYRVIPLAQVPIEIESLKTAFEQQKQHLTQGLAKLDPSTSHFELIEADILILD
DEELLAQIKDAISKLQIRAAAAIERIFTYQASELEQLDDPYLANRAEDILCLSSRLIARLHGAQHLDLSQLNQDTIIFAH
DLTPAEFAMLPLKHIKGLVLNTGGITSHTAILARSAGIPALLSCQFDGEQIKNGTQVVLNAIEGELVIKPSPEHLAQLYR
LQQQDIERKQQLLTLKDLPTETLDAHQVSLLANVGSLNEISHLADVGAEGIGLFRTEFMLMNTSILPDEKTQYHFYCDAL
HLMAGKTFTIRTLDVGADKELPCLTPLVEDNPALGMRGIRYSLANPQLFITQVKAILRAANHGPIRLMFPMINQVEELEA
LFDLIEACKLQLIEEEKGFGQLSYGIVVETPAAVLNLESMLPQLDFISIGTNDLTQYTMAADRTNPSLTSEYPSLSPAVL
HLIKTTIDLAQQHQVKVSLCGELASEPKVAPLLVGMGIDELSVNLSSLLEVKSAIRDISYQTCLALAKHAIKIKRIEELN
IYINGLN
>Mature_566_residues
SITGIVVSSGIAFGQALHLNLQHQKIDYRVIPLAQVPIEIESLKTAFEQQKQHLTQGLAKLDPSTSHFELIEADILILDD
EELLAQIKDAISKLQIRAAAAIERIFTYQASELEQLDDPYLANRAEDILCLSSRLIARLHGAQHLDLSQLNQDTIIFAHD
LTPAEFAMLPLKHIKGLVLNTGGITSHTAILARSAGIPALLSCQFDGEQIKNGTQVVLNAIEGELVIKPSPEHLAQLYRL
QQQDIERKQQLLTLKDLPTETLDAHQVSLLANVGSLNEISHLADVGAEGIGLFRTEFMLMNTSILPDEKTQYHFYCDALH
LMAGKTFTIRTLDVGADKELPCLTPLVEDNPALGMRGIRYSLANPQLFITQVKAILRAANHGPIRLMFPMINQVEELEAL
FDLIEACKLQLIEEEKGFGQLSYGIVVETPAAVLNLESMLPQLDFISIGTNDLTQYTMAADRTNPSLTSEYPSLSPAVLH
LIKTTIDLAQQHQVKVSLCGELASEPKVAPLLVGMGIDELSVNLSSLLEVKSAIRDISYQTCLALAKHAIKIKRIEELNI
YINGLN

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=565, Percent_Identity=38.2300884955752, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI48994992, Length=477, Percent_Identity=34.1719077568134, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1789193, Length=480, Percent_Identity=33.3333333333333, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1788726, Length=568, Percent_Identity=32.3943661971831, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1787994, Length=452, Percent_Identity=25.2212389380531, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 62490; Mature: 62359

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITGIVVSSGIAFGQALHLNLQHQKIDYRVIPLAQVPIEIESLKTAFEQQKQHLTQGLA
CCEEEEEEECCCCCCEEEEEEEEECCCCEEEEEHEECCCHHHHHHHHHHHHHHHHHHHHH
KLDPSTSHFELIEADILILDDEELLAQIKDAISKLQIRAAAAIERIFTYQASELEQLDDP
HCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
YLANRAEDILCLSSRLIARLHGAQHLDLSQLNQDTIIFAHDLTPAEFAMLPLKHIKGLVL
HHHCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEE
NTGGITSHTAILARSAGIPALLSCQFDGEQIKNGTQVVLNAIEGELVIKPSPEHLAQLYR
ECCCCCHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHEECCCCEEECCCHHHHHHHHH
LQQQDIERKQQLLTLKDLPTETLDAHQVSLLANVGSLNEISHLADVGAEGIGLFRTEFML
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHEEEE
MNTSILPDEKTQYHFYCDALHLMAGKTFTIRTLDVGADKELPCLTPLVEDNPALGMRGIR
EECCCCCCCCCCEEEEEHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
YSLANPQLFITQVKAILRAANHGPIRLMFPMINQVEELEALFDLIEACKLQLIEEEKGFG
EECCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QLSYGIVVETPAAVLNLESMLPQLDFISIGTNDLTQYTMAADRTNPSLTSEYPSLSPAVL
EEEECEEEECCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHH
HLIKTTIDLAQQHQVKVSLCGELASEPKVAPLLVGMGIDELSVNLSSLLEVKSAIRDISY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCH
QTCLALAKHAIKIKRIEELNIYINGLN
HHHHHHHHHHHHHEEEEEEEEEEECCC
>Mature Secondary Structure 
SITGIVVSSGIAFGQALHLNLQHQKIDYRVIPLAQVPIEIESLKTAFEQQKQHLTQGLA
CEEEEEEECCCCCCEEEEEEEEECCCCEEEEEHEECCCHHHHHHHHHHHHHHHHHHHHH
KLDPSTSHFELIEADILILDDEELLAQIKDAISKLQIRAAAAIERIFTYQASELEQLDDP
HCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
YLANRAEDILCLSSRLIARLHGAQHLDLSQLNQDTIIFAHDLTPAEFAMLPLKHIKGLVL
HHHCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEE
NTGGITSHTAILARSAGIPALLSCQFDGEQIKNGTQVVLNAIEGELVIKPSPEHLAQLYR
ECCCCCHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHEECCCCEEECCCHHHHHHHHH
LQQQDIERKQQLLTLKDLPTETLDAHQVSLLANVGSLNEISHLADVGAEGIGLFRTEFML
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHEEEE
MNTSILPDEKTQYHFYCDALHLMAGKTFTIRTLDVGADKELPCLTPLVEDNPALGMRGIR
EECCCCCCCCCCEEEEEHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
YSLANPQLFITQVKAILRAANHGPIRLMFPMINQVEELEALFDLIEACKLQLIEEEKGFG
EECCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QLSYGIVVETPAAVLNLESMLPQLDFISIGTNDLTQYTMAADRTNPSLTSEYPSLSPAVL
EEEECEEEECCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHH
HLIKTTIDLAQQHQVKVSLCGELASEPKVAPLLVGMGIDELSVNLSSLLEVKSAIRDISY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCH
QTCLALAKHAIKIKRIEELNIYINGLN
HHHHHHHHHHHHHEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]