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The map label for this gene is yebU

Identifier: 157961750

GI number: 157961750

Start: 2336976

End: 2338442

Strand: Reverse

Name: yebU

Synonym: Spea_1927

Alternate gene names: 157961750

Gene position: 2338442-2336976 (Counterclockwise)

Preceding gene: 157961752

Following gene: 157961740

Centisome position: 45.19

GC content: 44.38

Gene sequence:

>1467_bases
ATGGCTCATTTTAATCAGAATTTTCTCGATAGTATCGAACGCGATCTCCCTTCTCATCTTTCTATGGAAGACTTTATCGC
CTACAGCAATAAACCGTTACGACTTTCTATTCGAGTAAATACGCTCAAAATTAGTCACGATAACTTTATCGATTTAATGA
CGCCAAAAGGCTGGCACTTCGAGCCTATTCCTTGGTGTAAAGACGGCTTTTGGGTAACGCTCACTCAAGAGATCCAGCTA
GGTAATACCATCGAGCATTTGCAAGGATTGTTCTACATTCAGGAAGCCAGCTCCATGCTGCCTCCAACGGCACTATTTGA
GCCCCTAGAGGTTGCCGTGGCCGCAGAAAATACTGAGGATGATGGTTCCGATGACTTTTCCAATAAAATAGTCCTCGACA
TGGCTTCTGCACCAGGGTCAAAAACCACCCAGATTGCGGCACTGATGAAAAACCAAGGGCTGTTAATAGCCAATGAATAC
TCTGCTAGCCGAGTAAAAGTGCTGCACGCCAATGTGGCGAGGATGGGCGTGGCTAACTGCGCGTTAACCCATTTTGATGC
ACGAGTTTTCGGTGAGTATATGTTCGAAACATTTGATTCAATTCTACTCGATGCCCCTTGCAGCGGTGAAGGCACCATCC
GCAAAGATCCTGACGCACTAAAGAATTGGGATAATAATGATGACAAGGGCATTGTCGAGACCCAAAAAGCACTGATTGAA
TCTGCTTTTTTAGCATTAAAAGTCGGCGGCAGCCTTGTTTACTCGACCTGTGCGTTAAGCCGACAAGAAAACCAAGATGT
ATGTCACCACCTGAAATCTCTCTATGGTGATGCCGTTGAATTCGGTTCTTTGGCTACACTATTCCCACAGGCGGATAAGG
CTTGTACCGAAGAAGGCTTTTTACACGTATGGCCTCAGATCTACGACAGTGAAGGCTTCTTCGTAGCAAAAATATACAAA
ACCTCAGCAGTAGAACGTCAGCTGCCTGAGCCGAGAAAACAGAAGAACTTTCCATTTACATTGGCAAAACCTAAGCAAGT
CGAAGAGTTAGCACAATACTTTAGAGACAGCTTTTCTATTAGCCTACCCGATAATGCACAGGTGATGGTGCGCGATTTGG
AGTTTTGGCTGTTTCCAAGCCCTGTTATTCCATTAATAGGTAAGATGCGTTATCAACGGATAGGAATAAAACTCGCCGAT
GCGTTAAAGAAAGGCTACAAGGTTCGCCATGAAGCCGTTTTAGCTTTGTCATCCCCTACTCGTTTCGAACTCAGTGATGA
GCAAGCTAAAGAGTTTTTAATGGGTCGAGACATCAGCTTACTAGAAAAGGGGAAACCTCAAGGGGAAGTTATCGTCAGCT
ATGCTAGCAACCCGCTCGGTGTTGCTAAGCACTTAGGCAATAAATTAAAGAACAATCTTCCAAGAGACTTAGTCAAAGAT
AAGATTGCCCTGTATTCACATAATTAG

Upstream 100 bases:

>100_bases
TTATTTAGCACTCAAGTTCCCTCTAATCATCTATTTAAACCCATCTCATTAAATATAAACCTGTTATAATCTCGATACTT
TACCGCATTATCTTCGACAT

Downstream 100 bases:

>100_bases
TTACATTTGGGTGGCACTTCTAAATAAATAACCACCCACAATCCCTAAAGGTGGTCTACACTCTATATCATTAGTCTTTA
GCAATGATGTGATTAAACCT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MAHFNQNFLDSIERDLPSHLSMEDFIAYSNKPLRLSIRVNTLKISHDNFIDLMTPKGWHFEPIPWCKDGFWVTLTQEIQL
GNTIEHLQGLFYIQEASSMLPPTALFEPLEVAVAAENTEDDGSDDFSNKIVLDMASAPGSKTTQIAALMKNQGLLIANEY
SASRVKVLHANVARMGVANCALTHFDARVFGEYMFETFDSILLDAPCSGEGTIRKDPDALKNWDNNDDKGIVETQKALIE
SAFLALKVGGSLVYSTCALSRQENQDVCHHLKSLYGDAVEFGSLATLFPQADKACTEEGFLHVWPQIYDSEGFFVAKIYK
TSAVERQLPEPRKQKNFPFTLAKPKQVEELAQYFRDSFSISLPDNAQVMVRDLEFWLFPSPVIPLIGKMRYQRIGIKLAD
ALKKGYKVRHEAVLALSSPTRFELSDEQAKEFLMGRDISLLEKGKPQGEVIVSYASNPLGVAKHLGNKLKNNLPRDLVKD
KIALYSHN

Sequences:

>Translated_488_residues
MAHFNQNFLDSIERDLPSHLSMEDFIAYSNKPLRLSIRVNTLKISHDNFIDLMTPKGWHFEPIPWCKDGFWVTLTQEIQL
GNTIEHLQGLFYIQEASSMLPPTALFEPLEVAVAAENTEDDGSDDFSNKIVLDMASAPGSKTTQIAALMKNQGLLIANEY
SASRVKVLHANVARMGVANCALTHFDARVFGEYMFETFDSILLDAPCSGEGTIRKDPDALKNWDNNDDKGIVETQKALIE
SAFLALKVGGSLVYSTCALSRQENQDVCHHLKSLYGDAVEFGSLATLFPQADKACTEEGFLHVWPQIYDSEGFFVAKIYK
TSAVERQLPEPRKQKNFPFTLAKPKQVEELAQYFRDSFSISLPDNAQVMVRDLEFWLFPSPVIPLIGKMRYQRIGIKLAD
ALKKGYKVRHEAVLALSSPTRFELSDEQAKEFLMGRDISLLEKGKPQGEVIVSYASNPLGVAKHLGNKLKNNLPRDLVKD
KIALYSHN
>Mature_487_residues
AHFNQNFLDSIERDLPSHLSMEDFIAYSNKPLRLSIRVNTLKISHDNFIDLMTPKGWHFEPIPWCKDGFWVTLTQEIQLG
NTIEHLQGLFYIQEASSMLPPTALFEPLEVAVAAENTEDDGSDDFSNKIVLDMASAPGSKTTQIAALMKNQGLLIANEYS
ASRVKVLHANVARMGVANCALTHFDARVFGEYMFETFDSILLDAPCSGEGTIRKDPDALKNWDNNDDKGIVETQKALIES
AFLALKVGGSLVYSTCALSRQENQDVCHHLKSLYGDAVEFGSLATLFPQADKACTEEGFLHVWPQIYDSEGFFVAKIYKT
SAVERQLPEPRKQKNFPFTLAKPKQVEELAQYFRDSFSISLPDNAQVMVRDLEFWLFPSPVIPLIGKMRYQRIGIKLADA
LKKGYKVRHEAVLALSSPTRFELSDEQAKEFLMGRDISLLEKGKPQGEVIVSYASNPLGVAKHLGNKLKNNLPRDLVKDK
IALYSHN

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=310, Percent_Identity=34.1935483870968, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI76150623, Length=310, Percent_Identity=34.1935483870968, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI39995082, Length=397, Percent_Identity=23.6775818639798, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI301336155, Length=387, Percent_Identity=24.5478036175711, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI32698918, Length=148, Percent_Identity=35.8108108108108, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI11545785, Length=205, Percent_Identity=31.7073170731707, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI40316918, Length=189, Percent_Identity=30.6878306878307, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI87081985, Length=484, Percent_Identity=51.6528925619835, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI2367212, Length=191, Percent_Identity=29.8429319371728, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=318, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25143471, Length=280, Percent_Identity=30, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324268, Length=298, Percent_Identity=34.2281879194631, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319447, Length=252, Percent_Identity=30.5555555555556, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI22024126, Length=330, Percent_Identity=32.4242424242424, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21355201, Length=223, Percent_Identity=30.4932735426009, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24668781, Length=151, Percent_Identity=33.112582781457, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356513, Length=151, Percent_Identity=33.112582781457, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMF_SHEPA (A8H3W2)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501784.1
- ProteinModelPortal:   A8H3W2
- SMR:   A8H3W2
- STRING:   A8H3W2
- GeneID:   5662321
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1927
- NMPDR:   fig|398579.3.peg.1656
- eggNOG:   COG0144
- HOGENOM:   HBG726909
- OMA:   VANEYSA
- ProtClustDB:   PRK11933
- BioCyc:   SPEA398579:SPEA_1927-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01579
- InterPro:   IPR001678
- InterPro:   IPR011023
- TIGRFAMs:   TIGR00446

Pfam domain/function: PF01189 Nol1_Nop2_Fmu

EC number: =2.1.1.178

Molecular weight: Translated: 54754; Mature: 54623

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 257-257 BINDING 159-159 BINDING 186-186 BINDING 204-204

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHFNQNFLDSIERDLPSHLSMEDFIAYSNKPLRLSIRVNTLKISHDNFIDLMTPKGWHF
CCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEECCCCCCC
EPIPWCKDGFWVTLTQEIQLGNTIEHLQGLFYIQEASSMLPPTALFEPLEVAVAAENTED
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHEEEEHHHHCCCCHHHCCCCEEEEEECCCCC
DGSDDFSNKIVLDMASAPGSKTTQIAALMKNQGLLIANEYSASRVKVLHANVARMGVANC
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHH
ALTHFDARVFGEYMFETFDSILLDAPCSGEGTIRKDPDALKNWDNNDDKGIVETQKALIE
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHH
SAFLALKVGGSLVYSTCALSRQENQDVCHHLKSLYGDAVEFGSLATLFPQADKACTEEGF
HHHHHEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCHHHHCCCHHHHHHHCCC
LHVWPQIYDSEGFFVAKIYKTSAVERQLPEPRKQKNFPFTLAKPKQVEELAQYFRDSFSI
EEECCEEECCCCEEEEEEHHHHHHHHHCCCCHHHCCCCEEECCCHHHHHHHHHHHHCCEE
SLPDNAQVMVRDLEFWLFPSPVIPLIGKMRYQRIGIKLADALKKGYKVRHEAVLALSSPT
ECCCCCEEEEEEEHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCC
RFELSDEQAKEFLMGRDISLLEKGKPQGEVIVSYASNPLGVAKHLGNKLKNNLPRDLVKD
CCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHH
KIALYSHN
HHHHCCCC
>Mature Secondary Structure 
AHFNQNFLDSIERDLPSHLSMEDFIAYSNKPLRLSIRVNTLKISHDNFIDLMTPKGWHF
CCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEECCCCCCC
EPIPWCKDGFWVTLTQEIQLGNTIEHLQGLFYIQEASSMLPPTALFEPLEVAVAAENTED
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHEEEEHHHHCCCCHHHCCCCEEEEEECCCCC
DGSDDFSNKIVLDMASAPGSKTTQIAALMKNQGLLIANEYSASRVKVLHANVARMGVANC
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHH
ALTHFDARVFGEYMFETFDSILLDAPCSGEGTIRKDPDALKNWDNNDDKGIVETQKALIE
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHH
SAFLALKVGGSLVYSTCALSRQENQDVCHHLKSLYGDAVEFGSLATLFPQADKACTEEGF
HHHHHEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCHHHHCCCHHHHHHHCCC
LHVWPQIYDSEGFFVAKIYKTSAVERQLPEPRKQKNFPFTLAKPKQVEELAQYFRDSFSI
EEECCEEECCCCEEEEEEHHHHHHHHHCCCCHHHCCCCEEECCCHHHHHHHHHHHHCCEE
SLPDNAQVMVRDLEFWLFPSPVIPLIGKMRYQRIGIKLADALKKGYKVRHEAVLALSSPT
ECCCCCEEEEEEEHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCC
RFELSDEQAKEFLMGRDISLLEKGKPQGEVIVSYASNPLGVAKHLGNKLKNNLPRDLVKD
CCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHH
KIALYSHN
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA