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The map label for this gene is metG [H]

Identifier: 157961740

GI number: 157961740

Start: 2327899

End: 2329938

Strand: Reverse

Name: metG [H]

Synonym: Spea_1917

Alternate gene names: 157961740

Gene position: 2329938-2327899 (Counterclockwise)

Preceding gene: 157961750

Following gene: 157961736

Centisome position: 45.03

GC content: 45.2

Gene sequence:

>2040_bases
ATGGCAAATTCACAACGTAAAATCCTAGTCACAAGTGCACTTCCGTACGCTAACGGTCCAATCCATTTGGGTCATATGCT
TGAGTATATTCAAACTGATATTTGGTCACGTTTCCAAAAACTACGTGGGCATGAATGTCACTATATCTGCGCTGACGATG
CTCACGGCACGCCAATCATGCTTAAAGCACAGCAGCTGGGTATGACTCCTGAAGATATGATCGCTCAAGTTCAAAAAGAA
CATGAGAAGGATTTTGCTGACTTCAATATCCAGTTTGATAATTTCCACAGCACTCACAGTGATGAAAACCGTGAGCTAGC
GAGCGAGATCTATATCAAGCTACGCGATGCTGGCTATATCAAGACCAAGACGATTTCTCAGCTATTCGACCCAGAAAAGT
CGATGTTCCTTCCTGACCGTTTTGTTAAGGGCACTTGCCCACGTTGTAAGTCTGAAGATCAATATGGTGATAACTGCGAC
AACTGCGGTGCAACCTACAGCACGACCGATCTGATTGATCCAAAATCAGCGGTATCTGGCGCAACACCGGTAATGAAAGA
TACAGAGCATTTCTTCTTTGACCTTCCTTCTTTCGAAGGCATGCTTAAAGAGTGGATTAACTCAGGTTCTTTGCAGCAAG
AAATGGCTAACAAGCTAGGCGAGTGGTTCGAGCAAGGCCTACAGCAGTGGGATATCTCACGCGATGCGCCATACTTTGGT
TTTGAGATCCCAGATGCACCAGGTAAGTTCTTCTACGTTTGGTTAGACGCGCCTATCGGCTACATGGGCTCATTTAAGAA
CCTATGTAACAAGCGTGACGATCTAAACTTTGATGATTTCTGGAGCAAAGATTCAACTGCCGAAGTTTATCACTTCATCG
GTAAAGATATCGTTTACTTCCACAGTCTATTCTGGCCAGCAATGCTTGAAGGCGCTGGCTTACGTAAGCCAACCAGTGTT
TATGCACACGGTTATGTCACGGTTAACGGCGCTAAGATGTCAAAGTCTAAAGGCACCTTTATTAAGGCTCGTACATACCT
TGATAACCTAGATCCTGAATACCTACGTTATTACTACGCTGCCAAGCTGAGTAGCAGAATTGATGACCTTGACTTAAACC
TTGAAGACTTCGCTCAGCGTGTAAACTCTGATCTTGTTGGTAAGTTAGTCAACCTAGCCTCACGTACAGCTGGCTTTATC
AGCAAACGTTTTGATGGCAAGTTAGCAAAAATCGCAGACACTAGCCTGACAGAAAGCTTCTTAGCTAAACAAGAAACTAT
TGCTAACTTCTACGAGACTCGTGAATTCGGCAAAGCAATGCGTGAAGTGATGACACTTGCAGACATCGCTAACGCCTATG
TTGCAGACTCGGCACCTTGGCAGTTAATTAAAGATGACGCTAAGCAAGAAGAAGCGCACCAAGTATGCACTAATGCACTT
AACCTGTTCCGCATCTTGGTGACCTATCTTAAGCCAGTACTACCTAAGCTTGCACAAGATGTTGAAGCCTTCCTACAGAT
GGAACTAACCTGGGACAACCTAGACGTTGATCTAGCAGGCCATGAAATCGCTAAATTCAAGGCGTTAATGCAGCGTGTTG
AAATGAAGAGCATCGAAGCCATTATTGAAGCCTCTAAAGAGAACCTTCAAGTGACTGCTGAGCCTGAAGTTAAAAAGCAA
GATAAGACACCGCTTGAGCAAGACCCGATTAGCGAGGAGATCAGTTTCGAAGATTTCGCTAAGATCGATCTGCGCATCGC
ACGTATCGCCAAGGCTGAACACGTTAAAGAAGCCAATAAACTTCTACGTCTTGAGCTTGATTTAGGCGGCGAAACCAAGC
AAGTGTTTGCAGGTATTAAGTCGGCCTATGCTCCTGAAGATCTCGAAGGTAAACTTACTGTTATGGTAGCAAACCTAGCA
CCGCGCCAGATGCGCTTCGGTGTATCAGAAGGCATGGTATTGGCCGCAGGCCCTGGTGGAAAAGATCTGTGGATCATGGA
GCCACACGAAGGTGCCCAGCCTGGTATGAAAGTAAAATAA

Upstream 100 bases:

>100_bases
CTAGATTTAATCCACTTGGGCGAGCTTTTGATGAAACTATGCATTGGATCGGTTAGTATCTATGCCATTCCAATTAGGTT
CCCAGCGATTTTGAGAAAAG

Downstream 100 bases:

>100_bases
ACTTTTTTATTTATACTGATTGGTATTAAACGCTCTGTCCCGTCCTGAAATAGGTTGACGGTTTTTACTAGACCAGTAGC
TTCTGCTGCTGGTCTTTTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIMLKAQQLGMTPEDMIAQVQKE
HEKDFADFNIQFDNFHSTHSDENRELASEIYIKLRDAGYIKTKTISQLFDPEKSMFLPDRFVKGTCPRCKSEDQYGDNCD
NCGATYSTTDLIDPKSAVSGATPVMKDTEHFFFDLPSFEGMLKEWINSGSLQQEMANKLGEWFEQGLQQWDISRDAPYFG
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDDFWSKDSTAEVYHFIGKDIVYFHSLFWPAMLEGAGLRKPTSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDNLDPEYLRYYYAAKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFI
SKRFDGKLAKIADTSLTESFLAKQETIANFYETREFGKAMREVMTLADIANAYVADSAPWQLIKDDAKQEEAHQVCTNAL
NLFRILVTYLKPVLPKLAQDVEAFLQMELTWDNLDVDLAGHEIAKFKALMQRVEMKSIEAIIEASKENLQVTAEPEVKKQ
DKTPLEQDPISEEISFEDFAKIDLRIARIAKAEHVKEANKLLRLELDLGGETKQVFAGIKSAYAPEDLEGKLTVMVANLA
PRQMRFGVSEGMVLAAGPGGKDLWIMEPHEGAQPGMKVK

Sequences:

>Translated_679_residues
MANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIMLKAQQLGMTPEDMIAQVQKE
HEKDFADFNIQFDNFHSTHSDENRELASEIYIKLRDAGYIKTKTISQLFDPEKSMFLPDRFVKGTCPRCKSEDQYGDNCD
NCGATYSTTDLIDPKSAVSGATPVMKDTEHFFFDLPSFEGMLKEWINSGSLQQEMANKLGEWFEQGLQQWDISRDAPYFG
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDDFWSKDSTAEVYHFIGKDIVYFHSLFWPAMLEGAGLRKPTSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDNLDPEYLRYYYAAKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFI
SKRFDGKLAKIADTSLTESFLAKQETIANFYETREFGKAMREVMTLADIANAYVADSAPWQLIKDDAKQEEAHQVCTNAL
NLFRILVTYLKPVLPKLAQDVEAFLQMELTWDNLDVDLAGHEIAKFKALMQRVEMKSIEAIIEASKENLQVTAEPEVKKQ
DKTPLEQDPISEEISFEDFAKIDLRIARIAKAEHVKEANKLLRLELDLGGETKQVFAGIKSAYAPEDLEGKLTVMVANLA
PRQMRFGVSEGMVLAAGPGGKDLWIMEPHEGAQPGMKVK
>Mature_678_residues
ANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIMLKAQQLGMTPEDMIAQVQKEH
EKDFADFNIQFDNFHSTHSDENRELASEIYIKLRDAGYIKTKTISQLFDPEKSMFLPDRFVKGTCPRCKSEDQYGDNCDN
CGATYSTTDLIDPKSAVSGATPVMKDTEHFFFDLPSFEGMLKEWINSGSLQQEMANKLGEWFEQGLQQWDISRDAPYFGF
EIPDAPGKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDDFWSKDSTAEVYHFIGKDIVYFHSLFWPAMLEGAGLRKPTSVY
AHGYVTVNGAKMSKSKGTFIKARTYLDNLDPEYLRYYYAAKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFIS
KRFDGKLAKIADTSLTESFLAKQETIANFYETREFGKAMREVMTLADIANAYVADSAPWQLIKDDAKQEEAHQVCTNALN
LFRILVTYLKPVLPKLAQDVEAFLQMELTWDNLDVDLAGHEIAKFKALMQRVEMKSIEAIIEASKENLQVTAEPEVKKQD
KTPLEQDPISEEISFEDFAKIDLRIARIAKAEHVKEANKLLRLELDLGGETKQVFAGIKSAYAPEDLEGKLTVMVANLAP
RQMRFGVSEGMVLAAGPGGKDLWIMEPHEGAQPGMKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=632, Percent_Identity=28.7974683544304, Blast_Score=265, Evalue=9e-71,
Organism=Homo sapiens, GI24308436, Length=518, Percent_Identity=24.7104247104247, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI1788432, Length=682, Percent_Identity=65.6891495601173, Blast_Score=981, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=660, Percent_Identity=27.1212121212121, Blast_Score=247, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI212645096, Length=519, Percent_Identity=26.3969171483622, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=414, Percent_Identity=26.5700483091787, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=28.5204991087344, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321610, Length=542, Percent_Identity=23.0627306273063, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24655311, Length=563, Percent_Identity=29.8401420959147, Blast_Score=256, Evalue=4e-68,
Organism=Drosophila melanogaster, GI21358483, Length=511, Percent_Identity=25.440313111546, Blast_Score=136, Evalue=5e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76845; Mature: 76714

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIM
CCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGMTPEDMIAQVQKEHEKDFADFNIQFDNFHSTHSDENRELASEIYIKLRDAGYI
EEHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHEEEECCCCE
KTKTISQLFDPEKSMFLPDRFVKGTCPRCKSEDQYGDNCDNCGATYSTTDLIDPKSAVSG
EHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
ATPVMKDTEHFFFDLPSFEGMLKEWINSGSLQQEMANKLGEWFEQGLQQWDISRDAPYFG
CCCCCCCCCHHEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDDFWSKDSTAEVYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGAGLRKPTSVYAHGYVTVNGAKMSKSKGTFIKARTYLDNLDPEYLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEEEEHHHCCCCHHHHHHHHH
AKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFISKRFDGKLAKIADTSLTESF
HHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAKQETIANFYETREFGKAMREVMTLADIANAYVADSAPWQLIKDDAKQEEAHQVCTNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
NLFRILVTYLKPVLPKLAQDVEAFLQMELTWDNLDVDLAGHEIAKFKALMQRVEMKSIEA
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHH
IIEASKENLQVTAEPEVKKQDKTPLEQDPISEEISFEDFAKIDLRIARIAKAEHVKEANK
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLRLELDLGGETKQVFAGIKSAYAPEDLEGKLTVMVANLAPRQMRFGVSEGMVLAAGPGG
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCC
KDLWIMEPHEGAQPGMKVK
CEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ANSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRFQKLRGHECHYICADDAHGTPIM
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGMTPEDMIAQVQKEHEKDFADFNIQFDNFHSTHSDENRELASEIYIKLRDAGYI
EEHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHEEEECCCCE
KTKTISQLFDPEKSMFLPDRFVKGTCPRCKSEDQYGDNCDNCGATYSTTDLIDPKSAVSG
EHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
ATPVMKDTEHFFFDLPSFEGMLKEWINSGSLQQEMANKLGEWFEQGLQQWDISRDAPYFG
CCCCCCCCCHHEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCNKRDDLNFDDFWSKDSTAEVYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGAGLRKPTSVYAHGYVTVNGAKMSKSKGTFIKARTYLDNLDPEYLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEEEEHHHCCCCHHHHHHHHH
AKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFISKRFDGKLAKIADTSLTESF
HHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAKQETIANFYETREFGKAMREVMTLADIANAYVADSAPWQLIKDDAKQEEAHQVCTNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
NLFRILVTYLKPVLPKLAQDVEAFLQMELTWDNLDVDLAGHEIAKFKALMQRVEMKSIEA
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHH
IIEASKENLQVTAEPEVKKQDKTPLEQDPISEEISFEDFAKIDLRIARIAKAEHVKEANK
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLRLELDLGGETKQVFAGIKSAYAPEDLEGKLTVMVANLAPRQMRFGVSEGMVLAAGPGG
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCC
KDLWIMEPHEGAQPGMKVK
CEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA