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The map label for this gene is dadA [H]

Identifier: 157961531

GI number: 157961531

Start: 2072218

End: 2073480

Strand: Direct

Name: dadA [H]

Synonym: Spea_1706

Alternate gene names: 157961531

Gene position: 2072218-2073480 (Clockwise)

Preceding gene: 157961530

Following gene: 157961534

Centisome position: 40.05

GC content: 47.74

Gene sequence:

>1263_bases
ATGAATAATGCTCAACCTGACACACTGACCGTTGTAGGTGCTGGAATTGTGGGATTAGCCGCCGCAATTGAATTACAGCG
GGCAGGTTTTAACGTTACAGTGGTGGATAAAGAGGGGGCCGGCACGGGAGCCTCTAAGGGCAATGCGGGTCATTTTGCAA
CCGAACAAGTATTCCCTTTAGCTGATCCGGCCATGTTACCTAAACTACCGGGCATGCTGCTGGACCCACTAGGGCCGTTT
CGCATTCAACCTAGATACTTTTTAAAGGCGTTACCTTGGTTTTTGCGTTTTTTACACAATATGCTGCCTTCACGAAGAGT
TCAGAATAGCCGAGCGATTAAAGCACTTAATCAAGATTCAATCGCTGCGATGAAGTCCCTTACTGCGTTTTGTGGCTGTG
AAGAGCTGCTCACCTTGAACGGTAGCTTGCTGGTATTTGAAGGTACTCCGTTGCAGGAAGTACAGAAAGAGTTTCGCGCT
TATGCCGATGCAGGGGTGGAACTGCAGTTACTTTCTGGAGACGAAGTCAGAGCGCTAGAGCCATCCTTGACGACTAACAT
CACCAATGCCCTGTACTTTACTCATGTTGGTCATACAGAAGATCCCTATCGTTTATGTTTGGCACTTGAATCCAAATTTA
AAGAGATGGGCGGGAAGGTTGTTACCGAAGAGCTAGTTAAGATCAATACCTCTAATACAGATCCGAGCATTACCTTAGAG
ACTAACTCGGGTAGCCATAAAACCGAATGCCTGATAATCGCATCGGGGGCTTGGTCGAAACCTTTTGCTAAGCAGCTGGG
GTACTCGGTACCTTTGGAATCAGAGCGGGGCTACCATTTGATGATGCCACAGCTTAGCCGTTTGTCACGTCCGGTAGCTT
CTTATAACCGTAAGTTCATCATCACTCCTATGCGTGATGGCACTCGGCTAGCGGGCACCGTTGAGTTTGGTGGATTAAAA
GCACCATTAGTCGAGGCGAGAGCAAATTGTTTGTTTCCTCATGCTAAAGCACTGCTGCCAGAGCTGTTTGCTGATGCGAC
AGTTGAGGACGGTGTGCGTTGGATGGGCTTTAGACCTTCTATGCCTGATAGCCTGCCAGTTCTAGGGCGTAGTCAAAAGC
AATCTAATGTGTTCTTCTCATTCGGTCATCAACACTTAGGGCTGACTTGGTCGGCGATTACGGCAAAGTTATTGGCCCAA
GAGGTGCTAGGTAAACAAGCCGATATTGACTTAAGCCCCTATAGAATCGATAGGTTTAGTTAG

Upstream 100 bases:

>100_bases
ACCGGTAAGCACCAGTTCTTTATCGATCCTGACGATGTGTTCCAAAATGGCTTTATGTTGCGTTAATCGTGTCAAAGATA
ATTGAGTGGAGAAAAAGCTT

Downstream 100 bases:

>100_bases
AGCCTGCTATATGCTGTTTTGTAGACGTTGATTTAAAAATGGAGTTTTAAATGAATAAGTTTTAAAAGTTAATCTTTATC
GAGAGTAAGCAGAACAACTA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNNAQPDTLTVVGAGIVGLAAAIELQRAGFNVTVVDKEGAGTGASKGNAGHFATEQVFPLADPAMLPKLPGMLLDPLGPF
RIQPRYFLKALPWFLRFLHNMLPSRRVQNSRAIKALNQDSIAAMKSLTAFCGCEELLTLNGSLLVFEGTPLQEVQKEFRA
YADAGVELQLLSGDEVRALEPSLTTNITNALYFTHVGHTEDPYRLCLALESKFKEMGGKVVTEELVKINTSNTDPSITLE
TNSGSHKTECLIIASGAWSKPFAKQLGYSVPLESERGYHLMMPQLSRLSRPVASYNRKFIITPMRDGTRLAGTVEFGGLK
APLVEARANCLFPHAKALLPELFADATVEDGVRWMGFRPSMPDSLPVLGRSQKQSNVFFSFGHQHLGLTWSAITAKLLAQ
EVLGKQADIDLSPYRIDRFS

Sequences:

>Translated_420_residues
MNNAQPDTLTVVGAGIVGLAAAIELQRAGFNVTVVDKEGAGTGASKGNAGHFATEQVFPLADPAMLPKLPGMLLDPLGPF
RIQPRYFLKALPWFLRFLHNMLPSRRVQNSRAIKALNQDSIAAMKSLTAFCGCEELLTLNGSLLVFEGTPLQEVQKEFRA
YADAGVELQLLSGDEVRALEPSLTTNITNALYFTHVGHTEDPYRLCLALESKFKEMGGKVVTEELVKINTSNTDPSITLE
TNSGSHKTECLIIASGAWSKPFAKQLGYSVPLESERGYHLMMPQLSRLSRPVASYNRKFIITPMRDGTRLAGTVEFGGLK
APLVEARANCLFPHAKALLPELFADATVEDGVRWMGFRPSMPDSLPVLGRSQKQSNVFFSFGHQHLGLTWSAITAKLLAQ
EVLGKQADIDLSPYRIDRFS
>Mature_420_residues
MNNAQPDTLTVVGAGIVGLAAAIELQRAGFNVTVVDKEGAGTGASKGNAGHFATEQVFPLADPAMLPKLPGMLLDPLGPF
RIQPRYFLKALPWFLRFLHNMLPSRRVQNSRAIKALNQDSIAAMKSLTAFCGCEELLTLNGSLLVFEGTPLQEVQKEFRA
YADAGVELQLLSGDEVRALEPSLTTNITNALYFTHVGHTEDPYRLCLALESKFKEMGGKVVTEELVKINTSNTDPSITLE
TNSGSHKTECLIIASGAWSKPFAKQLGYSVPLESERGYHLMMPQLSRLSRPVASYNRKFIITPMRDGTRLAGTVEFGGLK
APLVEARANCLFPHAKALLPELFADATVEDGVRWMGFRPSMPDSLPVLGRSQKQSNVFFSFGHQHLGLTWSAITAKLLAQ
EVLGKQADIDLSPYRIDRFS

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=420, Percent_Identity=25.2380952380952, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45947; Mature: 45947

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAQPDTLTVVGAGIVGLAAAIELQRAGFNVTVVDKEGAGTGASKGNAGHFATEQVFPL
CCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ADPAMLPKLPGMLLDPLGPFRIQPRYFLKALPWFLRFLHNMLPSRRVQNSRAIKALNQDS
CCCHHHHCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHCCHHH
IAAMKSLTAFCGCEELLTLNGSLLVFEGTPLQEVQKEFRAYADAGVELQLLSGDEVRALE
HHHHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEC
PSLTTNITNALYFTHVGHTEDPYRLCLALESKFKEMGGKVVTEELVKINTSNTDPSITLE
CCHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCEEEEE
TNSGSHKTECLIIASGAWSKPFAKQLGYSVPLESERGYHLMMPQLSRLSRPVASYNRKFI
ECCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEECHHHHHHHCCHHHCCCEEE
ITPMRDGTRLAGTVEFGGLKAPLVEARANCLFPHAKALLPELFADATVEDGVRWMGFRPS
EEECCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCEEECCCCC
MPDSLPVLGRSQKQSNVFFSFGHQHLGLTWSAITAKLLAQEVLGKQADIDLSPYRIDRFS
CCCCCCCCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNAQPDTLTVVGAGIVGLAAAIELQRAGFNVTVVDKEGAGTGASKGNAGHFATEQVFPL
CCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ADPAMLPKLPGMLLDPLGPFRIQPRYFLKALPWFLRFLHNMLPSRRVQNSRAIKALNQDS
CCCHHHHCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHCCHHH
IAAMKSLTAFCGCEELLTLNGSLLVFEGTPLQEVQKEFRAYADAGVELQLLSGDEVRALE
HHHHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEC
PSLTTNITNALYFTHVGHTEDPYRLCLALESKFKEMGGKVVTEELVKINTSNTDPSITLE
CCHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCEEEEE
TNSGSHKTECLIIASGAWSKPFAKQLGYSVPLESERGYHLMMPQLSRLSRPVASYNRKFI
ECCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEECHHHHHHHCCHHHCCCEEE
ITPMRDGTRLAGTVEFGGLKAPLVEARANCLFPHAKALLPELFADATVEDGVRWMGFRPS
EEECCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCEEECCCCC
MPDSLPVLGRSQKQSNVFFSFGHQHLGLTWSAITAKLLAQEVLGKQADIDLSPYRIDRFS
CCCCCCCCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA